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Mepiquat chloride increases the Cry1Ac protein content of Bt cotton under high temperature and drought stress by regulating carbon and amino acid metabolism

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Journal of Integrative Agriculture 2024年第12期23卷 4032-4045页

作者： Dian Jin Yuting Liu Zhenyu Liu Yuyang Dai Jianing Du Run He tianfan wu Yuan Chen Dehua Chen Xiang Zhang Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009China

The effects of mepiquat chloride(DPC)on the Cry1Ac protein content in Bacillus thuringiensis(Bt)cotton boll shells under high temperature and drought stress were investigated to provide a theoretical reference for Bt cotton breeding and high-yield and-efficiency cotton *** study was conducted using Bt cotton cultivar‘Sikang 3'during the 2020 and 2021 growing seasons at Yangzhou University Farm,Yangzhou,Jiangsu Province,*** cotton plants were exposed to high temperature and drought stress,and sprayed with either 20 mg L^(-1)DPC or water(CK).Seven days after treatment,the Cry1Ac protein content,α-ketoglutarate content,pyruvic acid content,glutamate synthase activity,glutamic oxaloacetic transaminase activity,soluble protein content,and amino acid content were measured,and transcriptome sequencing was *** was used for differential gene *** the DPC treatment,the Cry1Ac protein content increased by 4.7-11.9% compared to ***α-ketoglutarate content,pyruvic acid content,glutamate synthase activity,glutamic oxaloacetic transaminase activity,soluble protein content,and amino acid content all *** analysis revealed 7,542 upregulated genes and 10,449 downregulated genes for DPC *** ontology(GO)and Kyoto Encyclopedia of Gene and Genomes(KEGG)analyses showed that the differentially expressed genes were mainly involved in biological processes,such as carbon and amino acid *** example,genes encoding 6-phosphofructokinase,pyruvate kinase,glutamic pyruvate transaminase,pyruvate dehydrogenase,citrate synthase,isocitrate dehydrogenase,2-oxoglutarate dehydrogenase,glutamate synthase,1-pyrroline-5-carboxylate dehydrogenase,glutamic oxaloacetic transaminase,amino-acid N-acetyltransferase,and acetylornithine deacetylase were all significantly *** DPC treatment increased pyruvate,α-ketoglutarate,and oxaloacetate by increasing the operational rate of the glycolytic pathway of the citric acid cycl

关键词： amino acid metabolism Bt cotton carbon metabolism Cry1Ac protein mepiquat chloride

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高温干旱下缩节胺通过调节碳和氨基酸代谢提高Bt棉杀虫蛋白含量的生理机制

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中国农业科学 2023年第8期56卷 1471-1483页

作者：邢羽桐滕永康吴天凡刘媛媛陈源陈媛陈德华张祥扬州大学农学院/江苏省作物遗传生理国家重点实验室培育点

【目的】探讨高温干旱胁迫下缩节胺（mepiquat chloride,DPC）调控Bt(Bacillus thuringiensis)棉杀虫蛋白含量的生理机制，为高抗虫性Bt棉品种选育及高产高效栽培提供理论依据。【方法】2020—2021年以转Bt抗虫基因抗虫棉品种泗抗3号为... 详细信息

【目的】探讨高温干旱胁迫下缩节胺（mepiquat chloride,DPC）调控Bt(Bacillus thuringiensis)棉杀虫蛋白含量的生理机制，为高抗虫性Bt棉品种选育及高产高效栽培提供理论依据。【方法】2020—2021年以转Bt抗虫基因抗虫棉品种泗抗3号为材料，采用盆栽法，在人工气候室进行高温干旱胁迫，胁迫开始后立即用20 mg·L-1 DPC和清水（对照）喷施。7 d后测定铃壳杀虫蛋白含量、α-酮戊二酸含量、丙酮酸含量以及谷氨酸合酶活性、谷氨酸草酰乙酸转氨酶活性、可溶性蛋白含量、游离氨基酸含量。并进行转录组测序，利用DESeq进行差异基因分析，通过GO富集和KEGG Pathway数据库注释参与DPC调节杀虫蛋白含量的差异表达基因。【结果】与清水对照相比，DPC可显著提高高温干旱条件下Bt棉铃壳中杀虫蛋白含量，提高幅度达4.7%—11.9%。在碳代谢方面，α-酮戊二酸含量、丙酮酸含量提高46%—57%和25%—29%；在氨基酸代谢方面，谷氨酸合酶活性、谷氨酸草酰乙酸转氨酶活性、可溶性蛋白含量、游离氨基酸含量分别提高32%—44%、30%—40%、28%和22%—27%。转录组分析结果表明，DPC处理后上调基因7 542条，下调基因10 449条。GO和KEGG结果显示，差异基因主要涉及氨基酸代谢、碳代谢等生物过程。其中编码6-磷酸果糖激酶和丙酮酸激酶、谷氨酸丙酮酸转氨酶、丙酮酸脱氢酶、柠檬酸合酶、异柠檬酸脱氢酶和α-酮戊二酸脱氢酶、谷氨酸合酶、吡咯啉-5-羧酸脱氢酶、谷氨酸草酰乙酸转氨酶、N-乙酰谷氨酸合成酶、乙酰鸟氨酸脱乙酰酶基因表达显著上调。【结论】高温干旱下，DPC通过调节碳、氨基酸代谢增加丙氨酸、α-酮戊二酸含量，提高天冬氨酸、谷氨酸、丙酮酸和精氨酸合成能力，进而提高Bt棉杀虫蛋白含量。

关键词： Bt棉缩节胺杀虫蛋白氨基酸代谢碳代谢

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昼夜变温下高温与干旱胁迫对Bt棉毒蛋白含量的影响及其生理机制

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中国农业科学 2022年第23期55卷 4614-4625页

作者：尹彦雨邢雨桐吴天凡王李妍赵子胥胡天然陈源陈媛陈德华张祥扬州大学/江苏省作物遗传生理国家重点实验室培育点

【目的】明确Bt棉叶片Cry1Ac毒蛋白含量对昼夜变温下高温干旱胁迫响应及其生理机制，为生产中Bt棉抗虫性的安全稳定利用提供参考。【方法】2019—2020年在扬州大学农学院，以常规种泗抗1号（SK-1）和杂交种泗抗3号（SK-3）为材料，以温... 详细信息

【目的】明确Bt棉叶片Cry1Ac毒蛋白含量对昼夜变温下高温干旱胁迫响应及其生理机制，为生产中Bt棉抗虫性的安全稳定利用提供参考。【方法】2019—2020年在扬州大学农学院，以常规种泗抗1号（SK-1）和杂交种泗抗3号（SK-3）为材料，以温度和土壤水分含量为因子，温度分别设为34℃（白天，7:00—19:00）/28℃（夜间，19:00—7:00）(A1)、38℃/28℃（A2）；土壤水分含量分别为田间土壤最大持水量的50%(B1)和60%(B2)，并以32℃/28℃、田间土壤最大持水量的75%为对照（CK）。各处理分别持续4、7、10 d(DAS)。【结果】不同处理导致叶片中Cry1Ac毒蛋白含量降低，且随着胁迫时间的延长，下降幅度增加。处理间相比，A1B2处理下降幅度最少，7 DAS后开始显著低于CK;A1B1处理下降幅度其次，4 DAS后显著低于CK;A2B1、A2B2处理在4 DAS显著下降。可溶性蛋白（SP）含量、硝酸还原酶（NR）、谷氨酸丙酮酸转氨酶（GPT）、谷氨酸草酰乙酸转氨酶（GOT）、谷氨酰胺合成酶（GS）、游离氨基酸（aa）和谷氨酸合成酶（GOGAT）等活性变化趋势与毒蛋白一致，且呈极显著正相关，而Bt基因表达量、单宁含量、蛋白酶、肽酶活性则呈上升趋势。逐步回归和通径分析筛选出NR、GPT、GS等3个关键指标可反映Bt棉Cry1Ac毒蛋白含量高低，且三者对Cry1Ac毒蛋白含量有较大的正效应。【结论】昼夜变温下高温和干旱互作导致Bt棉Cry1Ac毒蛋白含量降低，且随持续期延长下降幅度逐渐增大。其中34℃/28℃和田间土壤最大持水量60%胁迫7—10 d内与对照无显著差异。但与昼夜持续高温相比，昼夜变温的高温胁迫下Cry1Ac毒蛋白含量下降幅度减小和时期明显推迟。NR、GPT、GS是决定Cry1Ac毒蛋白含量高低的关键指标。

关键词： Bt棉 Cry1Ac毒蛋白高温干旱生理机制

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Do multiple cycles of aeolian deposit-pedogenesis exist in the reticulate red clay sections in southern China?

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Chinese Science Bulletin 2003年第2期48卷 1251-1258页

作者： Xuefeng Hu tianfan Cheng Hexin wu Shanghai Univ Dept Environm Sci & Engn Shanghai 200072 Peoples R China

The Xiangyang reticulate red clay section was newly sampled in high-resolution. The viewpoints different from the previous studies[17] are put forward after studying Munsell color values, weathering degrees, magnetic parame-ters and stable carbon isotopic compositions of the section. They are as follows: (1) The curves of hue, DCB extracted iron (Fed), DCB extracted iron/total iron ratio (Fed/Fet), weathering index (BA) of the section show that the redness is not significantly correlated with the weathering degree of the layers though the uppermost yellow-brown-colored layer is relatively weakly weathered. The variation in hues of the section is possibly attributed to the change of hematite and goethite contents. It is insufficient to determine the existence of the multiple deposit-pedogenesis cycles in the section, like the loess-paleosol sequence in Chinese Loess Plateau, only by the color variation. (2) Magnetic susceptibility (c) values in the non-reticulate and weakly reticulate layers are high; but are quite low in the reticulate red clay layer. The former are more than ten times higher than the latter because of the leaching and collapse of superparamagnetic (SP) and stable single domain (SSD) magnetic particles during the plinthitic processes. Hence, c values in the section are not controlled by weathering and pedogenic processes, and no longer have clear paleoclimatic implication. It is not correct to prove the existence of the multiple deposit-pedogenesis cycles in the section using c curves. (3) The organic d 13C curve of the section illustrates the reduction of forests and increasing of C4 plants in the study area since the late stage of the Qua-ternary period. It could not prove the existence of the multi-ple deposit-pedogenesis cycles in the section either. (4) As a matter of fact, it is difficult to prove the existence of the mul-tiple deposit-pedogenesis cycles in the Xiangyang section like the loess-paleosol sequence in Chinese Loess Plateau using t

关键词： reticulate red clay Xianyyang reticulate red clay section (Xiangyang section) c weathering degree.

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Plasmonic tandem heterojunctions enable high-efficiency charge transfer for broad spectrum photocatalytic hydrogen production

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Journal of Energy Chemistry 2025年第1期100卷 710-720页

作者： Wenxuan Chen Xiu-Qing Qiao Guo Hui Bojing Sun Dongfang Hou Meidi Wang Xueqian wu Tao wu Dong-Sheng Li College of Materials and Chemical Engineering Key Laboratory of Inorganic Nonmetallic Crystalline and Energy Conversion Materials China Three Gorges University Hubei Three Gorges Laboratory College of Chemistry and Materials Science and Guangdong Provincial Key Laboratory of Supramolecular Coordination Chemistry Jinan University

Rational engineering of semiconductor photocatalysts for efficient hydrogen production is of great significance but still challenging,primarily due to the limitations in charge transfer ***,a fascinating plasmonic tandem heterojunction with the hc-CdS/Mo2C@C heterostructure is aimfully prepared for effectively promoting the charge separation kinetics of the CdS photocatalyst via the synergistic strategy of phase junction,Schottky junction,and photothermal *** difference in atomic configuration between cubic-CdS （c-CdS） and hexagonal-CdS （h-CdS） leads to effective charge separation through a typical Ⅱ charge transfer mechanism,and plasmonic Schottky junction further extracts the electrons in the hc-CdS phase junction to realize gradient charge ***,the photothermal effect of Mo2C@C helps to expand the light absorption,accelerate charge transfer kinetics,and reduce the hydrogen evolution energy *** carbon layer provides a fast channel for charge transfer and protects the photocatalyst from *** a result,the optimized hc-CMC photocatalyst exhibits a significantly high photocatalytic H2production activity of 28.63 mmol/g/h and apparent quantum efficiency of 61.8%,surpassing most of the reported *** study provides a feasible strategy to enhance the charge transfer kinetics and photocatalytic activity of CdS by constructing plasmonic tandem heterogeneous junctions.

关键词： Heterojunctions LSPR Photocatalyst Phase junction Schottky junction Photothermal effect

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Sex-dependent alterations in extracellular vesicles linking chronic spinal cord injury to brain neuroinflammation and neurodegeneration

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Neural Regeneration Research 2025年第2期20卷 483-484页

作者： Yun Li Junfang wu Department of Anesthesiology&Shock Trauma and Anesthesiology Research(STAR)CenterUniversity of Maryland School of MedicineBaltimoreMDUSA

Traumatic spinal cord injury(SCI)is a devastating exogenous injury with long-lasting consequences and a leading cause of death and disability *** in assistive technology,rehabilitative interventions,and the ability to identify and intervene in secondary conditions have significantly increased the long-term survival rate of SCI patients,with some people even living well into their seventh or eighth *** survival changes have led neurotrauma researchers to examine how SCI interacts with brain *** health and epidemiological data showed that patients with long-term SCI can have a lower life expectancy and quality of life,along with a higher risk of comorbidities and complications.

关键词： alterations inflammation injury

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Ammonia pyrolysis oxidation excited by nanosecond pulsed discharge:Global/fluid models hybrid solution

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Journal of Energy Chemistry 2025年第1期100卷 385-396页

作者： Mingming Zhang Hanyu wu Qi Chen Jiaxin Yang Bonan Liu School of Mechanical Electronic and Control Engineering Beijing Jiaotong University China University of Petroleum

The kinetic characteristics of plasma-assisted oxidative pyrolysis of ammonia are studied by using the global/fluid models hybrid solution ***,the stable products of plasma-assisted oxidative pyrolysis of ammonia are *** results show that the consumption of NH3/O2and the production of N2/H2change linearly with the increase of voltage,which indicates the decoupling of nonequilibrium molecular excitation and oxidative pyrolysis of ammonia at low ***,the detailed reaction kinetics mechanism of ammonia oxidative pyrolysis stimulated by a nanosecond pulse voltage at low pressure and room temperature is *** on the reaction path analysis,the simplified mechanism is *** detailed and simplified mechanism simulation results are compared with experimental data to verify the accuracy of the simplified ***,based on the simplified mechanism,the fluid model of ammonia oxidative pyrolysis stimulated by the nanosecond pulse plasma is established to study the pre-sheath/sheath behavior and the resultant consumption and formation of key *** results show that the generation,development,and propagation of the pre-sheath have a great influence on the formation and consumption of *** consumption of NH3by the cathode pre-sheath is greater than that by the anode pre-sheath,but the opposite is true for OH and O（1S）.However,within the sheath,almost all reactions do not ***,by changing the parameters of nanosecond pulse power supply voltage,it is found that the electron number density,electron current density,and applied peak voltages are not the direct reasons for the structural changes of the sheath and ***,the discharge interval has little effect on the sheath structure and gas mixture *** research results of this paper not only help to understand the kinetic promotion of non-equilibrium excitation in the process of oxidative pyrolysis but also help to explore th

关键词： Nanosecond pulsed discharge Ammonia Oxidative pyrolysis Fluid model Global model

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缩节胺运筹对夏直播棉熟相的影响

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扬州大学学报（农业与生命科学版） 2024年第1期45卷 33-40页

作者：徐泽芮秋治刘震宇李涵佳冷鑫华吴天凡陈媛张祥陈源陈德华扬州大学江苏省作物遗传生理国家重点实验室培育点/粮食作物现代产业技术协同创新中心江苏扬州225009

为明确缩节胺运筹对夏直播棉熟相形成的影响,2018-2019年于扬州大学实验农场以中棉425为材料,设缩节胺用量0、180、360 g·hm^(-2)3个水平,施用时期分别设盛蕾期+初花期(施用比例1∶2)、盛蕾+初花期+盛花期(施用比例1∶2∶3) 2个运筹方... 详细信息

为明确缩节胺运筹对夏直播棉熟相形成的影响,2018-2019年于扬州大学实验农场以中棉425为材料,设缩节胺用量0、180、360 g·hm^(-2)3个水平,施用时期分别设盛蕾期+初花期(施用比例1∶2)、盛蕾+初花期+盛花期(施用比例1∶2∶3) 2个运筹方式,同时设常规施用方式(初花期,缩节胺120 g·hm^(-2))为对照,探讨缩节胺施用量和施用时期处理对此种植方式下熟相的调节。结果表明:缩节胺用量180 g·hm^(-2)并于盛蕾期+初花期或盛蕾+初花期+盛花期使用后再通过脱叶催熟吐絮率均能达到90%以上,且吐絮强度高,较对照显著提高46.6%~71.4%。进一步回归分析表明,黄叶率保持在9.0%~12.8%之间,主茎红茎比为52.8%~53.1%,倒1果枝红茎比为28.3%~34.3%,倒2果枝红茎比为27.5%~39.8%,倒3果枝红茎比为34.3%~8.5%,倒4果枝红茎比为49.1%~51.5%,吐絮倒序位为7.1~8.1台最有利于集中吐絮。因此,缩节胺合理运筹能形成集中吐絮的熟相,为实现机采提供技术支撑。

关键词：棉花夏直播棉缩节胺运筹集中吐絮熟相

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Brain-derived neurotrophic factor plays with TRiC:focus on synaptic dysfunction in Huntington’s disease

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Neural Regeneration Research 2025年第10期20卷 2919-2920页

作者： Yingli Gu Kijung Sung Chengbiao wu Department of Neurosciences University of California San DiegoLa JollaCAUSA

Brain-derived neurotrophic factor(BDNF)exerts pleiotropic effects on brain processes including psychiatric disorders,aging,neurodegeneration,and metabolic homeostasis.A simple PubMed search using the key word“BDNF,”to date,yields over 33,000 *** fundamental biology to potential therapeutic applications,BDNF has clearly garnered extensive and significant attention in the field of neurobiology research.

关键词： Huntington homeostasis therapeutic

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Neuronal regulated cell death in aging-related neurodegenerative diseases:key pathways and therapeutic potentials

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Neural Regeneration Research 2025年第8期20卷 2245-2263页

作者： Run Song Shiyi Yin Jiannan wu Junqiang Yan Department of Neurology The First Affiliated HospitalCollege of Clinical Medicine of Henan University of Science and TechnologyLuoyangHenan ProvinceChina Neuromolecular Biology Laboratory The First Affiliated HospitalCollege of Clinical Medicine of Henan University of Science and TechnologyLuoyangHenan ProvinceChina

Regulated cell death(such as apoptosis,necroptosis,pyroptosis,autophagy,cuproptosis,ferroptosis,disulfidptosis)involves complex signaling pathways and molecular effectors,and has been proven to be an important regulatory mechanism for regulating neuronal aging and ***,excessive activation of regulated cell death may lead to the progression of aging-related *** review summarizes recent advances in the understanding of seven forms of regulated cell death in age-related ***,the newly identified ferroptosis and cuproptosis have been implicated in the risk of cognitive impairment and neurodegenerative *** forms of cell death exacerbate disease progression by promoting inflammation,oxidative stress,and pathological protein *** review also provides an overview of key signaling pathways and crosstalk mechanisms among these regulated cell death forms,with a focus on ferroptosis,cuproptosis,and *** instance,FDX1 directly induces cuproptosis by regulating copper ion valency and dihydrolipoamide S-acetyltransferase aggregation,while copper mediates glutathione peroxidase 4 degradation,enhancing ferroptosis ***,inhibiting the Xc-transport system to prevent ferroptosis can increase disulfide formation and shift the NADP^(+)/NADPH ratio,transitioning ferroptosis to *** insights help to uncover the potential connections among these novel regulated cell death forms and differentiate them from traditional regulated cell death *** conclusion,identifying key targets and their crosstalk points among various regulated cell death pathways may aid in developing specific biomarkers to reverse the aging clock and treat age-related neurodegenerative conditions.

关键词： apoptosis autophagy cuproptosis disulfidptosis ferroptosis necroptosis neurodegenerative disease neurological aging diseases PANoptosis pyroptosis

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