Mepiquat chloride increases the Cry1Ac protein content of Bt cotton under high temperature and drought stress by regulating carbon and amino acid metabolism

作     者：Dian Jin Yuting Liu Zhenyu Liu Yuyang Dai Jianing Du Run He Tianfan Wu Yuan Chen Dehua Chen Xiang Zhang Dian Jin;Yuting Liu;Zhenyu Liu;Yuyang Dai;Jianing Du;Run He;Tianfan Wu;Yuan Chen;Dehua Chen;Xiang Zhang

作者机构：Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain CropsYangzhou UniversityYangzhou 225009China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2024年第23卷第12期

页      面：4032-4045页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China(31901462) the Natural Science Foundation of the Jiangsu Higher Education Institutions,China(22KJA210005) the Priority Academic Program Development of Jiangsu Higher Education Institutions,China(PAPD) the Brand Professional Construction Program of Jiangsu Higher Education Institutions,China 

主　　题：amino acid metabolism Bt cotton carbon metabolism Cry1Ac protein mepiquat chloride 

摘      要：The effects of mepiquat chloride(DPC)on the Cry1Ac protein content in Bacillus thuringiensis(Bt)cotton boll shells under high temperature and drought stress were investigated to provide a theoretical reference for Bt cotton breeding and high-yield and-efficiency cotton *** study was conducted using Bt cotton cultivar‘Sikang 3 during the 2020 and 2021 growing seasons at Yangzhou University Farm,Yangzhou,Jiangsu Province,*** cotton plants were exposed to high temperature and drought stress,and sprayed with either 20 mg L^(-1)DPC or water(CK).Seven days after treatment,the Cry1Ac protein content,α-ketoglutarate content,pyruvic acid content,glutamate synthase activity,glutamic oxaloacetic transaminase activity,soluble protein content,and amino acid content were measured,and transcriptome sequencing was *** was used for differential gene *** the DPC treatment,the Cry1Ac protein content increased by 4.7-11.9% compared to ***α-ketoglutarate content,pyruvic acid content,glutamate synthase activity,glutamic oxaloacetic transaminase activity,soluble protein content,and amino acid content all *** analysis revealed 7,542 upregulated genes and 10,449 downregulated genes for DPC *** ontology(GO)and Kyoto Encyclopedia of Gene and Genomes(KEGG)analyses showed that the differentially expressed genes were mainly involved in biological processes,such as carbon and amino acid *** example,genes encoding 6-phosphofructokinase,pyruvate kinase,glutamic pyruvate transaminase,pyruvate dehydrogenase,citrate synthase,isocitrate dehydrogenase,2-oxoglutarate dehydrogenase,glutamate synthase,1-pyrroline-5-carboxylate dehydrogenase,glutamic oxaloacetic transaminase,amino-acid N-acetyltransferase,and acetylornithine deacetylase were all significantly *** DPC treatment increased pyruvate,α-ketoglutarate,and oxaloacetate by increasing the operational rate of the glycolytic pathway of the citric acid cycl