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Melatonin ameliorates experimental hepatic fibrosis induced by carbon tetrachloride in rats

World Journal of Gastroenterology 2009年第12期15卷 1452-1458页

作者： Ru-Tao Hong,Department of Geriatrics Medicine,The First Affiliated Hospital of anhui Medical University,hefei 23.022,anhui province,china Ru-Tao Hong,Jian-Ming Xu,Qiao Mei,Department of Gastroenterology,The First Affiliated Hospital of anhui Medical University,hefei 23.022,anhui province,china the key laboratory of digestive diseases of anhui province,hefei 23.022,anhui province,china Department of Geriatrics Medicine The First Affiliated Hospital of Anhui Medical University Hefei 230022 Anhui Province China.

AIM:To investigate the protective effects of melatonin on carbon tetrachloride(CCl4)-induced hepatic fibrosis in experimental ***:All rats were randomly divided into normal control group,model control group treated with CCl4 for 12 wk,CCl4+NAC group treated with CCl4+NAC(100 mg/kg,i.p.)for 12 wk,CCl4+MEL-1 group treated with CCl4+melatonin(2.5 mg/kg)for 12 wk,CCl4+MEL-2 group treated with CCl4+ melatonin(5.0 mg/kg)for 12 wk,and CCl4+MEL-3 group treated with CCl4+melatonin(10 mg/kg).Rats in the treatment groups were injected subcutaneously with sterile CCl4(3 mL/kg,body weight)in a ratio of 2:3 with olive oil twice a *** in normal control group received hypodermic injection of olive oil at the same dose and frequency as those in treatment *** the end of experiment,rats in each group were anesthetized and *** and eosin(HE)staining and Van Gieson staining were used to examine changes in liver *** activities of alanine aminotransferase(ALT),aspartate aminotransferase(AST)and protein concentration weremeasured with routine laboratory methods using an ***(HYP)content in liver and malondialdehyde(MDA)and glutathione peroxidase(GPx)levels in liver homogenates were assayed by *** hyaluronic acid(HA),laminin(LN),and procollagenⅢN-terminal peptide(PⅢNP)were determined by ***:Pathologic grading showed that the fibrogenesis was much less severe in CCl4+MEL3 group than in model control group(u=2.172,P<0.05),indicating that melatonin(10 mg/kg)can significantly ameliorate CCl4-induced hepatic fibrotic *** serum levels of ALT and AST were markedly lower in CCl4+MEL treatment groups(5,10 mg/kg)than in model control group(ALT:286.23 ±121.91 U/L vs 201.15±101.16 U/L and 178.67 ±103.14 U/L,P=0.028,P=0.007;AST:431.00 ±166.35 U/L vs 321.23±162.48 U/L and 292.42 ±126.23 U/L,P=0.043,P=0.013).Similarly,the serum laminin(LN)and hyaluronic acid(HA)levels and hydroxyproli

关键词： Melatonin Hepatic fibrosis Oxidativestress Hyaluronic acid Laminin Malondialdehyde Glutathione peroxidase

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Upregulation of TNF-αand IL-6 mRNA in mouse liver induced by bacille Calmette-Guerin plus lipopolysaccharide

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Acta Pharmacologica Sinica 2006年第4期27卷 460-468页

作者： Dao-fang LIU~(1,2,3) Wei WEI~(1,4) Li-hua SONG~(1,2,3,4) ~1Institute of Clinical Pharmacology,key laboratory of Anti-inflammatory and Immunopharmacology in anhui province.key laboratory of Research and Development of Chinese Medicine in anhui province.anhui Medical University,hefei 230032,china:~2Molecular Biology laboratory,anhui Biology Institute,hefei 230088,china ~3Center of Research and Development,anhui Anke Biotechnology Group,hefei 230088.china Institute of Clinical Pharmacology Key Laboratory of Anti-inflammatory and Immunopharmacology in Anhui Province Key Laboratory of Research and Development of Chinese Medicine in Anhui Province Anhui Medical University Hefei China Molecular Biology laboratory Anhui Biology Institute Hefei China Center of Research and Development Anhui Anke Biotechnology Group Hefei China

Aim:To investigate the mechanism of immunological liver injury induced by bacilleCalmette-Guerin(BCG)plus lipopolysaccharide(LPS).Methods:Mice were in-jected via the tail vein with 125 mg/kg BCG,and 12 d later,the mice were injectedintravenously with different doses of LPS(125,250,or 375 μg/kg).Serum alanineaminotransferase(ALT)activity and liver pathological changes were *** expression of tumor necrosis factor(TNF)-α interleukin(IL)-6,lipopolysac-charide binding protein(LBP)and CD14 mRNA,and NF-κB and IκB-α protein inmouse liver at different time points after BCG and LPS injection were measuredusing RT-PCR,immunohistochemistry and Western blotting analysis,***:The activity of serum ALT in mice treated with BCG and LPS was signifi-cantly *** degrees of liver injury,such as inflammatory cellinfiltration,spotty necrosis,piecemeal necrosis,even bridging necrosis,could beseen in liver sections from mice after BCG and LPS ***,thelevels of TNF-α and IL-6 mRNA in mouse liver were significantly elevated afteradministration of BCG plus LPS(P<0.05).The levels of LBP and CD14 mRNA inmouse liver were markedly upregulated after treatment with BCG and LPS,andtreatment with BCG alone led to an increase in CD14 InRNA in mouse ***,immunoreactivity for NF-κB p65 was predominantly detected in hepatocyte nucleifrom mice treated with BCG plus LPS,compared with the normal *** of IκB-α were strikingly decreased by LPS or BCG plus LPS treatment,compared with the normal group or BCG ***:TNF-α and IL-6mRNA were partially involved in early immunological liver injury induced by chal-lenge with small doses of LPS after BCG *** of TNF-α and IL-6 mRNA might be related to increases in LBP and CD 14 mRNA expression andactivation of NF-κ***,BCG priming in immunological liver injury mayoccur via upregulation of CD 14 mRNA expression in monon uclear cell infiltrationinto t

关键词： liver injury lipopolysaccharide binding protein CD14 tumor necrosis factor-alpha interleukin-6 NF-kappa B

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Research on EM pulse protection property of plasma-microwave absorptive material-plasma sandwich structure

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Science china(Technological Sciences) 2010年第12期53卷 3221-3224页

作者： YUAN ZhongCai & SHI JiaMing State key laboratory of Pulse Power Laser Technology, key Lab of Infrared and Low Temperature Plasma of anhui province, hefei Electronic Engineering Institute, hefei 230037, china State key laboratory of Pulse Power Laser Technology Key Lab of Infrared and Low Temperature Plasma of Anhui Province Hefei Electronic Engineering Institute Hefei China

A plasma-microwave absorptive material (MAM)-plasma sandwich structure is presented to protect the electronic device against high power electromagnetic pulse. The model of electromagnetic wave reflected by and transmitting through the structure is established. Based on the characteristic parameters of plasma generated by discharge and usual MAM, the electromagnetic transmissive properties of the sandwich structure are investigated by the method of finite difference in time domain. The results indicate that in a rather broad frequency range, the electromagnetic attenuations by the structure are obviously better than the sum of attenuations resulted from plasma and MAM respectively. The models and results presented are instructive for electromagnetic pulse protection.

关键词： plasma microwave absorptive material (MAM) sandwich structure electromagnetic pulse finite difference time domain

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Preliminary research on myosin light chain kinase in rabbit liver

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World Journal of Gastroenterology 2001年第6期7卷 868-871页

作者： Bin Ren~1 Hua-Qing Zhu~2 Zhao-Feng Luo~1 Qing Zhou~2 Yuan Wang~2 Yu-Zhen Wang~1 1 Department of Biochemistry and Molecular Biology,University of Science and Technology of china,hefei 230027,anhui province,china2 laboratory of Molecular Biology,anhui Medical University,hefei 230032,anhui province,china Department of Biochemistry and Molecular Biology University of Science and Technology of China Hefei 230027 Anhui Province China.

AIM: To study preliminarily the properties of myosin light chain kinase (MLCK) in rabbit liver. METHODS: The expression of MLCK was detected by reverse transcription-polymerase chain reaction(RT-PCR); the MLCK was obtained from rabbit liver, and its activity was analyzed by gamma-(32)P incorporation technique to detect the phosphorylation of myosin light chain. RESULTS: MLCK was expressed in rabbit liver, and the activity of the enzyme was similar to rabbit smooth muscle MLCK, and calmodulin-dependent. When the concentration was 0.65 mg x L(-1), the activity was at the highest level. CONCLUSION: MLCK expressed in rabbit liver may catalyze the phosphorylation of myosin light chain, which may play important roles in the regulation of hepatic cell functions.

关键词： Animals Hepatocytes Liver Myosin Light Chains Myosin-Light-Chain Kinase Phosphorylation Rabbits Research Support, Non-U.S. Gov't

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Interleukin-1 receptor antagonist intervenes in signaling between different types of synoviocytes in rats with adjuvant arthritis

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Acta Pharmacologica Sinica 2006年第1期27卷 111-118页

作者： Yong-qiu ZHENG~2 Wei WEI~(2,3,4) Min DAI~2 Lei ZHU~2 Xiao-yi JIA~2 Yuan WANG1 Institute of Clinical Pharmacology,anhui Medical University.hefei 230032,china 3 key laboratory of Anti-inflammation and Immunopharmacology in anhui province,hefei 230032.china Institute of Clinical Pharmacology Anhui Medical University Hefei China key laboratory of Anti-inflammation and Immunopharmacology in anhui province Hefei China

Aim:To investigate the mechanisms of interleukin-1 receptor antagonist （IL-1ra）in the treatment of adjuvant arthritis （AA）.Methods:AA was induced in rats bytreatment with Freund's complete adjuvant （FCA）.Rats were given an intracuta-neous injection of IL-1ra （2.5,10,40mg/kg,3 times per day） from d 14 to d 21 *** proliferation and the activity of IL-1 were determinedby using MTT *** necrosis factor alpha （TNF-α） and prostaglandin E2（PGE2） concentrations were measured by *** ultrastructureof synoviocytes was observed by using a transmission electron *** of c-Jun N-terminal kinase （JNK）,extracellular regulating kinase（ERK） and p38 kinase were detected by Western blot ***:IL-1ra （10and 40 mg/kg,ic,d 14-21） modulated the secondary inflammatory reaction（P<0.01）,ultrastructure of synoviocytes and mitogen-activated protein kinase（MAPK） phosphorylation in AA *** administration of IL-1ra （10 and 40mg/kg,ic,d 14-21） in AA rats significantly decreased the production of IL-1,PGE2and TNF-α by macrophage-like synoviocytes （MLS）（P<0.01）.IL-1ra （2.5 mg/kg）also decreased the production of PGE2（P<0.01） and TNF-α（P<0.05） by MLS inAA *** increased phosphorylation of MAPK and cell proliferation in fibro-blast-like synoviocytes （FLS） stimulated by supernatants of MLS in AA rats wasalso inhibited by IL-1ra （10 and 40 mg/kg,ic.d 14-21）.Conclusion:IL-1ra hasanti-inflammatory effects because it modulates the ultrastructure of synoviocytes,decreases the production of pro-inflammatory mediators by MLS,and inhibits thephosphorylation of MAPK in FLS.

关键词： interleukin-1 receptor experimental arthritis mitogen-activated protein kinase synovial membrane

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Research and Application of Integration Design Environment Faced to CSCD

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武汉理工大学学报 2006年第S2期28卷 690-693页

作者： ZHAO Han LI Yanfeng DONG Yude (key Library of Digital Design and Manufacture of anhui province,hefei University of Technology,hefei 230009,china key Library of Digital Design and Manufacture of anhui province Hefei University of TechnologyHefei 230009China

Computer supported collaborative design (CSCD) technology has been applied extensively with intensive market *** key technologies and problems of CSCD are analyzed and a CSCD design frame faced to product design is estab- *** a CSCD system faced to radar key components is founded with Pro/INTRALINK software and re-exploiting technolo- *** key processes are also designed,such as database management,work flow programming,information communication, file release,conflict identification and safety *** will provide a reference for constructing a cooperative design en- vironment.

关键词： CSCD integration design Pro/INTRALINK re-exploitation

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Study on transmittance spectra of ITO thin films by ellipsometric method

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Science china(Technological Sciences) 2010年第7期53卷 1893-1896页

作者： SUN ZhaoQi1, CAO ChunBin123, CAI Qi1 & SONG XuePing1 1 School of Physics and Material Science, anhui University, hefei 230039, china 2 School of Sciences, anhui Agricultural University, hefei 230036, china 3 key laboratory of Opto-electronic Information Acquisition and Manipulation, Ministry of Education, hefei 230036, china School of Physics and Material Science Anhui University Hefei China School of Sciences Anhui Agricultural University Hefei China key laboratory of Opto-electronic Information Acquisition and Manipulation Ministry of Education Hefei China

ITO films with thicknesses (134+8) nm, grown on glass substrates by sputtering method, were post-annealed at the temperatures of 100, 200, 300 and 400°C for 1 h, respectively. The as-deposited ITO film was amorphous, but crystallized with annealing at elevated temperatures, as demonstrated by X-ray diffraction. The transmittance spectra of all samples were obtained and subsequently simulated by means of spectroscopic ellipsometry. The optical constants n and k of the films were extracted. With the annealing temperature increasing, the optical constants n and k of the films firstly decreased then increased in the whole investigated wavelength range. The optical band gaps of all films were evaluated and they varied between 3.74 and 3.93 eV.

关键词： ITO films optical constants transmittance spectra spectroscopic ellipsometry analysis

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Synergistic effect of combining paeonol and cisplatin on apoptotic induction of human hepatoma cell lines

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Acta Pharmacologica Sinica 2007年第6期28卷 869-878页

作者： Shu-ping XU~2,Guo-ping SUN~(4,5),Yu-xian SHEN~2,Wan-ren PENG~4,Hua WANG~3,Wei WEI~2~2 Institute of Clinical Pharmacology,anhui Medical University,key laboratory of Anti-inflammatory and Immunological Pharmacology,key laboratory of Research and Development of Chinese Medicine,hefei 230032,china ~3 Department of Oncology,Provincial Hospital ofanhui,hefei 230001,china ~4 Department of Oncology,The First Affiliated Hospital of anhui Medical University,hefei 23.022,china Institute of Clinical Pharmacology Anhui Medical University Key Laboratory of Anti-inflammatory and Immunological Pharmacology Key Laboratory of Research and Development of Chinese Medicine Hefei China Department of Oncology The First Affiliated Hospital of Anhui Medical University Hefei China Department of Oncology Provincial Hospital of Anhui Hefei China

Aim:To investigate whether paeonol （Pae） has synergistic effects with cisplatin（CDDP） on the growth-inhibition and apoptosis-induction of human hepatomacell lines HepG2and ***:The cytotoxic effect of drugswas measured by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl-tetrazolium *** coefficient of drug interaction was used to analyze the nature of *** changes were observed by acridine orange fluo-rescence *** cycle and the apoptosis rate were detected by ***-2 and Bax expression were assayed by ***:Pae or CDDP had antiproliferative effect on the 2 cell linesin a dose-dependent manner,with different sensitivities to ***,a synergistic inhibitory effect on the viability of the 2 cell lineswas observed after treatment with a combination of Pae （15.63,31.25,and 62.5mg/L） with various concentrations of *** study showed typical mor-phological changes of apoptosis if the cells were exposed to the two agents for24 *** apoptotic rate of the cells with combination treatment was signifi-cantly higher than that of cells treated with Pae or CDDP *** expressionof Bcl-2 decreased and that of Bax increased in the treated groups,especially inthe combination group,with the ratio of Bcl-2/Bax decreasing ***,a combination of Pae with CDDP resulted in a stronger S phasearrest,compared with Pae or CDDP ***:Pae,in combinationwith CDDP,had a significantly synergistic growth-inhibitory and apoptosis-in-ducing effect on the 2 human hepatoma cell lines,which may be useful inhepatoma treatment.

关键词： paeonol cisplatin drug interaction human hepatoma cell line apoptosis cell cycle

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G protein-coupled signal transduction in synoviocytes of immune arthritis

G protein-coupled signal transduction in synoviocytes of imm...

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第十五届国际药理学大会

作者： Wei Wei Zhang Lingling.Institute of Clinical Pharmacology anhui Medical University key laboratory of Anti - inflammatory and immunopharmacology in anhui province key laboratory of Research and Development of Chinese Medicine in anhui province hefei 230032 china

＜正＞ G proteins are partners of G - protein - coupled receptors （GPCRs）. GPCRs catalyze guanine nucleotide exchange on Gα subunits,enabling both activated Gα and Gβγ subunits to target downst ream effector. Diverse extracellular signals regulate receptors to modulate cellular physiology. GPCRs signaling via heterotrimericG proteins is attenuated rapidly by G protein - coupled receptor kinase （GRK）. GPCRs phosphorylation is to promote the binding of arrestin proteins which block interactions of receptors and G - proteins. Regulators of Gprotein signaling are GTPase - activating proteins that attenuate signaling by G proteins. G proteins-AC- cAMP signal transduction play a crucial role in pathogenesis of immune arthritis. Gαs mRNA. protein express and function were decreased, and Gai mRNA. protein express and function of were increased in synoviocytes of rats with immune arthritis. The " cross - talk" was found between MAPK signal transduction and G proteins associated signal transduction. Activation of MAPKs was regulated by Gαi and Gαs signal transduction pathway. G proteins transmembrane signal pathway became new target for treatment of arthritis arthritis.

关键词： G protein MAPK signal transduction arthritis

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Microstructure and optical properties of nano Ag-ITO films

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Science china(Technological Sciences) 2010年第5期53卷 1266-1270页

作者： XIAO Lei1,3,CAO ChunBin2,3,SONG XuePing1,3,MENG FanMing1,3 & SUN ZhaoQi1,3 1 School of Physics and Material Science,anhui University,hefei 230039,china 2 School of Sciences,anhui Agricultural University,hefei 230036,china 3 key laboratory of Opto-Electronic Information Acquisition and Manipulation,Ministry of Education,hefei 230039,china School of Physics and Material Science Anhui University Hefei China key laboratory of Opto-Electronic Information Acquisition and Manipulation Ministry of Education Hefei China School of Sciences Anhui Agricultural University Hefei China

Nano Ag-ITO films with Ag volume fraction of 0.3%-1.0% were prepared by radio-frequency magnetron co-sputtering and analyzed by X-ray diffraction,scanning electron microscopy and ultraviolet-visible *** analysis shows that the films are composed of polycrystalline ITO matrix embedded with Ag nanoparticles with a mean size of 60-100 *** spectra of Ag-ITO films indicate that the visible light transmissivity of the films decreases with increasing the Ag *** transmissivity of the annealed films is higher than that of the as-deposited *** volume 0.3% Ag-ITO films have the highest light *** annealed films exhibit lower light absorptance than as-deposited films.A surface plasmon resonance(SPR) peak of volume 0.3% Ag-ITO films is located around 510 *** with the annealed ITO film,the annealed volume 0.3% Ag-ITO film shows 10% higher reflectivity,while its transmittance is almost the same as that of the annealed ITO film,indicating its potential application in new-type transflective displays.

关键词： nanoparticle films microstructure optical properties transflective displays

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