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Studies on anti-HIV quinolone-3-carboxyiic acids:new SAR insights on the C-5 position

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Studies on anti-HIV quinolone-3-carboxyiic acids:new SAR ins...

2011年全国药物化学学术会议——药物的源头创新

作者： Qiu-Qin He~a,Xuan Zhang~b,Hai-Qiu Wu~a,Shuang-Xi Gu~a,iao-Dong Ma~a,Liu-Meng Yang~b,Yong-Tang Zheng~b,and Fen-Er Chena,~(c,*) a Department of Chemistry,Fudan University,Shanghai 200433,PR China b key laboratory of animal Models and human disease Mechanisms of Chinese Academy of Sciences & Yunnan province,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming 650223,PR China c Institute of Biomedical Science,Fudan University,Shanghai 200433,PR China

＜正＞With the aim to explore the effect of chemical modifications at C-5 position of quinolone core,a series of 5-substitued（F,OH,OCH3） quinolone-3-carboxylic acids was prepared and compared to the 5-hydrogen counterparts for antiviral activity against HIV-1ⅢB in C8166 cell *** biological results showed that the effect of fluoro,hydroxyl,and methoxyl group at C-5 position was greatly influenced by the choice of N-l substituent.

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思茅松松塔提取物体外抗HIV-1研究

中草药 2016年第11期47卷 1914-1918页

作者：崔雪青刘熙陈欢彭芳罗荣华杨柳萌刘光明王睿睿郑永唐中国科学院和云南省动物模型与人类疾病机理重点实验室中国科学院昆明动物研究所云南昆明650223 大理大学药学与化学学院云南大理671000 云南中医学院中药学院云南昆明650500

目的研究思茅松松塔提取物（SMS-E和SMS-F）体外对HIV-1的抑制活性和作用机制。方法采用MTT比色法检测思茅松松塔提取物对C8166细胞的毒性；合胞体抑制实验、p24抑制实验和MT-4细胞保护作用检测提取物对HIV-1的抑制作用。采用HIV-1复制... 详细信息

目的研究思茅松松塔提取物（SMS-E和SMS-F）体外对HIV-1的抑制活性和作用机制。方法采用MTT比色法检测思茅松松塔提取物对C8166细胞的毒性；合胞体抑制实验、p24抑制实验和MT-4细胞保护作用检测提取物对HIV-1的抑制作用。采用HIV-1复制中间产物检测、融合阻断实验和分时给药实验判断提取物的作用机制。结果思茅松松塔提取物SMS-E和SMS-F对HIV-1有很好的抑制作用，对HIV-1_IIIB在C8166细胞中复制的治疗指数（TI）分别为436.46和558.61。HIV复制中间产物检测实验显示SMS-F处理组ssDNA和Late-RT的拷贝数明显低于对照组，提示作用于病毒复制的早期；融合阻断实验显示SMS-F对慢性H9／HIV-1_IlIB与C8166细胞融合有较好的抑制作用，EC_50值为71.03μg∥mL，说明SMS-F作用于病毒进入阶段；分时给药实验显示提取物对已进入细胞的HIV-1没有抑制作用，再次证明了SMS-F作用于HIV进入细胞阶段。结论思茅松松塔提取物SMS-F对HIV-1有很强的抑制活性，其作用机制为抑制HIV-1进入细胞阶段。

关键词：松塔思茅松艾滋病 HIV 融合阻断病毒进入

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四川省云阳县自然灾害概述

山地研究 1993年第2期11卷 97-100页

作者：唐治诚全永明彭华明四川省云阳县农业局四川云阳634500

为改善本县生态环境、发展县内贫困山区经济,论述了全县的自然灾害类型及其成因和危害,并提出了防灾减灾措施。

关键词：四川云阳县自然灾害滑坡洪水

Design,Synthesis and Structure-Activity Relationships of N-Phenylarylformamide Derivatives（PAFAs） as Non-nucleoside HIV Reverse Transcriptase Inhibitors

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Design,Synthesis and Structure-Activity Relationships of N-P...

2011年全国药物化学学术会议——药物的源头创新

作者： Xiao-Dong Ma~a,Qiu-Qin He~a,Xuan Zhang~(b,c),Shi-Qiong Yang~a,Liu-Meng Yang~b,Shuang-Xi Gu~a, Yong-Tang Zheng~b,Fen-Er Chen~(a,d,*) a Department of Chemistry,Fudan University,Shanghai 200433,PR China b key laboratory of animal Models and human disease Mechanisms of Chinese Academy of Sciences & Yunnan province,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming 650223,PR China c Graduate School of the Chinese Academy of Sciences,Beijing 100039,PR China d Institute of Biomedical Science,Fudan University,Shanghai 200031,PR China

＜正＞A series of N-phenylarylformamide derivatives（PAFAs） with anti-wild-type HIV-1 EC50 values ranging from 0.3 nM to 5.1 nM and selectivity index values ranging from 10616 to 271000 were identified as novel non-nucleoside reverse transcriptase *** them,compound 13g （EC50 = 0.30 nM,SI = 184578）,131（EC50 = 0.37 nM,SI = 212819）,13m(EC50 = 0.32 nM,SI =

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Methodological characteristics in establishing rat models of poststroke depression

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Neural Regeneration Research 2006年第9期1卷 829-832页

作者： Fuyou Liu1, Shi Yang2, Weiyin Chen1, Jinyu Wang1, Yi Tang1, Guanxiang Zhu1 1Department of Neurology, Affiliated Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu 610072, sichuan province, China 2Department of Neurology, First People’s Hospital of Chongqing City, Chongqing 400011, China Department of Neurology Chengdu University of Traditional Chinese Medicine Chengdu 610072 Sichuan Province China Department of Neurology First People's Hospital of Chongqing City Chongqing 400011 China

BACKGROUND: Ideal model of poststroke depression (PSD) may be induced in rats guided by the theoretical evidence that 'primary endogenous mechanism' and 'reactivity mechanism' theories for PSD in human being. OBJECTIVE: To investigate the feasibility of comprehensive methods to induce PSD models in rats. DESIGN: A randomized controlled animal trial. SETTING: Department of Neurology, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine. MATERIALS: Male SD rats of SPF degree, weighing 350-500 g, were provided by the experimental animal center of Chengdu University of Traditional Chinese Medicine. The rats were raised for 1 week adaptively, then screened behaviorally by open-field test and passive avoidance test. Forty-five rats with close scores were randomly divided into normal control group (n =10), simple stroke group (n =10), stress group (n =10) and PSD group (n =15). METHODS: The experiments were carried out in the laboratory of Chengdu University of Traditional Chinese Medicine from July 2002 to February 2003. ① Rat models of focal cerebral ischemia were induced by thread embolization, then treated with separate raising and unpredictable stress to induce PSD models. ②The neurologic deficit was evaluated by Longa 5-grade standard (the higher the score, the severer the neurologic deficit) and horizontal round rod test (normal rat could stay on it for at least 3 minutes). ③ The behavioral changes of PSD rats were evaluated by the saccharin water test, open-field text and passive avoidance test, including the changes of interest, spontaneous and exploratory activities, etc. ④ The levels of monoamine neurotransmitters, including norepinephrine (NE), serotonin (5-HT) and dopamine, in brain were determined using fluorospectrophotometry. MAIN OUTCOME MEASURES: ① Score of Longa 5-grade standard; Stayed time in the horizontal round rod test; ② Amount of saccharin water consumption; Open-field text: time stayed in the central square, times wondering a

关键词： Traditional Chinese Medicine focal cerebral ischemia neurologic deficit water consumption internal carotid artery poststroke depression brain stem experimental animal water test rat model

2005-2009年广汉市联江村血吸虫病疫情监测

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寄生虫病与感染性疾病 2011年第1期9卷 17-19页

作者：邓启华代凯黎攀陈静广汉市疾病预防控制中心四川广汉618300

目的掌握平坝地区血吸虫病监测点的疫情情况,为血吸虫病防治提供科学依据。方法按照《全国血吸虫病监测方案》的要求,对广汉市联江村监测点的血吸虫病疫情进行调查。结果 2005-2009年钉螺总面积从10 710 m2下降到7 870 m2,活螺密度前4... 详细信息

目的掌握平坝地区血吸虫病监测点的疫情情况,为血吸虫病防治提供科学依据。方法按照《全国血吸虫病监测方案》的要求,对广汉市联江村监测点的血吸虫病疫情进行调查。结果 2005-2009年钉螺总面积从10 710 m2下降到7 870 m2,活螺密度前4年比较稳定,但2009年回升较多为1.17只/框(每框=0.11m2),5年来未发现感染性钉螺,钉螺主要分布在沟渠和稻田等环境。2005-2009年人群血清阳性率从7.44%下降到3.73%,人群血吸虫感染率从0.52%下降到0。2005-2009年家畜耕牛除2006年查出3头病牛,阳性率30%以外,其余年份均未查出阳性病牛。结论 2005-2009年广汉市人群血吸虫病感染率、家畜血吸虫感染率、钉螺总面积均下降,血吸虫病疫情得到较好控制。

关键词：血吸虫病疫情监测

Convergent Neuroimaging and Molecular Signatures in Mild Cognitive Impairment and Alzheimer’s disease:A Data-Driven Meta-Analysis with N=3,118

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Neuroscience Bulletin 2024年第9期40卷 1274-1286页

作者： Xiaopeng Kang Dawei Wang Jiaji Lin Hongxiang Yao Kun Zhao Chengyuan Song Pindong Chen Yida Qu Hongwei Yang Zengqiang Zhang Bo Zhou Tong Han Zhengluan Liao Yan Chen Jie Lu Chunshui Yu Pan Wang Xinqing Zhang Ming Li Xi Zhang Tianzi Jiang Yuying Zhou Bing Liu Ying Han Yong Liu The Alzheimer’s disease Neuroimaging Initiative The Multi-Center Alzheimer’s disease Imaging(MCADI)Consortium School of Artificial Intelligence University of Chinese Academy of SciencesBeijing100049China Brainnetome Center and National laboratory of Pattern Recognition Institute of AutomationChinese Academy of SciencesBeijing100190China Department of Radiology Qilu Hospital of Shandong UniversityJi’nan250063China Department of Neurology the Second Affiliated Hospital of Air Force Medical UniversityXi’an710032China Department of Radiology Chinese PLA General HospitalBeijing100853China Department of Radiology the Second Medical CentreNational Clinical Research Centre for Geriatric DiseasesChinese PLA General HospitalBeijing100853China School of Artificial Intelligence Beijing University of Posts and TelecommunicationsBeijing100191China Department of Neurology Qilu Hospital of Shandong UniversityJi’nan250063China Department of Radiology Xuanwu Hospital of Capital Medical UniversityBeijing100053China Branch of Chinese PLA General HospitalSanya572013China Department of Neurology the Second Medical CentreNational Clinical Research Centre for Geriatric DiseasesChinese PLA General HospitalBeijing100853China Department of Radiology Tianjin Huanhu HospitalTianjin300222China Department of Psychiatry People’s Hospital of Hangzhou Medical CollegeZhejiang Provincial People’s HospitalHangzhou310014China Department of Radiology Tianjin Medical University General HospitalTianjin300070China Department of Neurology Tianjin Huanhu HospitalTianjin300222China Department of Neurology Xuanwu Hospital of Capital Medical UniversityBeijing100053China key laboratory of animal Models and human disease Mechanisms of the Chinese Academy of Sciences&Yunnan province Kunming Institute of ZoologyChinese Academy of SciencesKunming650201YunnanChina State key Lab of Cognition Neuroscience&Learning Beijing Normal UniversityBeijing100875China National Clinical Research Center for Geriatric Disorders Beijing100053China Center of Alzheimer’s disease Beijing Institute for Brain DisordersBeijing1000

The current study aimed to evaluate the susceptibility to regional brain atrophy and its biological mechanism in Alzheimer’s disease(AD).We conducted data-driven meta-analyses to combine 3,118 structural magnetic resonance images from three datasets to obtain robust atrophy *** we introduced a set of radiogenomic analyses to investigate the biological basis of the atrophy patterns in *** results showed that the hippocampus and amygdala exhibit the most severe atrophy,followed by the temporal,frontal,and occipital lobes in mild cognitive impairment(MCI)and *** extent of atrophy in MCI was less severe than that in AD.A series of biological processes related to the glutamate signaling pathway,cellular stress response,and synapse structure and function were investigated through gene set enrichment *** study contributes to understanding the manifestations of atrophy and a deeper understanding of the pathophysiological processes that contribute to atrophy,providing new insight for further clinical research on AD.

关键词： Alzheimer’s disease Structural magnetic resonance imaging Meta-analysis Brain atrophy Gene set enrichment analysis

Control of Malignant Arrhythmias by microRNAs

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Control of Malignant Arrhythmias by microRNAs

2008年全国生物化学与分子生物学学术大会

作者： Bao-feng Yang Yan-jie Lu Hong-li Shan Chao-qian Xu Yong Zhang Zhen-wei Pan Bao-xin Li (Department of Pharmacology,State-province key Laboratories of Biomedicine-Pharmaceutics of China,Harbin Medical University,Harbin 150081,China)

Aim:This study was designed to investigate whether MicroRNA (miRNA) is involved in pathological processes relevant to cardiomyocyte apoptosis and human cardiac disease,in addition to its role in regulating ***:animal ...

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Isolation and genetic characterization of avian origin H9N2 influenza viruses from pigs in China

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Isolation and genetic characterization of avian origin H9N2 ...

中国畜牧兽医学会兽医公共卫生学分会成立大会暨第一次学术研讨会

作者： Hai Yu~1 Rong-Hong Hua~1 Tian-Chao Wei~(1,3) Yan-Jun Zhou~(1,2) Zhi-Jun Tian~1 Guo-Xin Li~1 Tian-Qiang Liu~1 Guang-Zhi Tong~(1,2*) 1 National key laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China 2 Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200232,China 3 College of animal Science and Technology,Guangxi University,Nanning 530005,China

As pigs are susceptible to infection with both avian and human influenza A-viruses,they have been proposed to be an intermediate host for the adaptation of avian influenza viruses to *** April 2006,a disease caused by highly pathogenic porcine reproductive and respiratory syndrome virus(PRRSV)occurred in several pig farms and subsequently overwhelmed almost half of China with more than 2,000,000 cases of pig *** we report a case in which four swine H9N2 influenza viruses were isolated from pigs infected by highly pathogenic PRRSVs in Guangxi province in China. All the eight gene segments of the four swine H9N2 viruses arc highly homologous to A/Pigeon/Nanchang/2-0461/00 (H9N2)or A/Wild Duck/Nanchang/2-0480100(H9N2).Phylogenetic analyses of eight genes show that the swine H9N2 influenza viruses arc of avian origin and may be the descendants of A/Duck/Hong Kong/Y280/97-like *** analysis of the HA gene indicates that our H9N2 isolates might have high-affinity binding to the a2,6-NeuAcGal receptor found in human *** conclusion,our finding provides further evidence about the interspccies transmission of avian influenza viruses to pigs and emphasizes the importance of reinforcing swine influenza virus surveillance,especially after the emergence of highly pathogenic PRRSVs in pigs in China.

关键词： Swine influenza Avian H9N2 influenza virus Porcine reproductive and respiratory syndrome virus Genetic analysis Molecular analysis