Methodological characteristics in establishing rat models of poststroke depression

作     者：Fuyou Liu1, Shi Yang2, Weiyin Chen1, Jinyu Wang1, Yi Tang1, Guanxiang Zhu1 1Department of Neurology, Affiliated Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan Province, China 2Department of Neurology, First People’s Hospital of Chongqing City, Chongqing 400011, China 

作者机构：Department of Neurology Chengdu University of Traditional Chinese Medicine Chengdu 610072 Sichuan Province China Department of Neurology First People's Hospital of Chongqing City Chongqing 400011 China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2006年第1卷第9期

页      面：829-832页

核心收录：

学科分类：1002[医学-临床医学] 100205[医学-精神病与精神卫生学] 10[医学] 

基　　金：a grant from the Natural Scientific Research Key Program of Sichuan Bureau of Science and Technology  No.02SG011-32 

主　　题：Traditional Chinese Medicine focal cerebral ischemia neurologic deficit water consumption internal carotid artery poststroke depression brain stem experimental animal water test rat model 

摘      要：BACKGROUND: Ideal model of poststroke depression (PSD) may be induced in rats guided by the theoretical evidence that primary endogenous mechanism and reactivity mechanism theories for PSD in human being. OBJECTIVE: To investigate the feasibility of comprehensive methods to induce PSD models in rats. DESIGN: A randomized controlled animal trial. SETTING: Department of Neurology, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine. MATERIALS: Male SD rats of SPF degree, weighing 350-500 g, were provided by the experimental animal center of Chengdu University of Traditional Chinese Medicine. The rats were raised for 1 week adaptively, then screened behaviorally by open-field test and passive avoidance test. Forty-five rats with close scores were randomly divided into normal control group (n =10), simple stroke group (n =10), stress group (n =10) and PSD group (n =15). METHODS: The experiments were carried out in the laboratory of Chengdu University of Traditional Chinese Medicine from July 2002 to February 2003. ① Rat models of focal cerebral ischemia were induced by thread embolization, then treated with separate raising and unpredictable stress to induce PSD models. ②The neurologic deficit was evaluated by Longa 5-grade standard (the higher the score, the severer the neurologic deficit) and horizontal round rod test (normal rat could stay on it for at least 3 minutes). ③ The behavioral changes of PSD rats were evaluated by the saccharin water test, open-field text and passive avoidance test, including the changes of interest, spontaneous and exploratory activities, etc. ④ The levels of monoamine neurotransmitters, including norepinephrine (NE), serotonin (5-HT) and dopamine, in brain were determined using fluorospectrophotometry. MAIN OUTCOME MEASURES: ① Score of Longa 5-grade standard; Stayed time in the horizontal round rod test; ② Amount of saccharin water consumption; Open-field text: time stayed in the central square, times wondering a