检索结果-南通市图书馆

Tithonia diversifoliaMulch Stimulates the Growth of Plantain PIF Seedlings and Induces a Less Susceptibility to Mycosphaerella fijiensisin the Nursery

引用

American Journal of Plant Sciences 2020年第5期11卷 672-692页

作者： Arouna Meshuneke Cécile Annie ewané Robinson Nembot Tatsegouock thaddée boudjeko Laboratory of Phytoprotection and Plant Valorization Biotechnology Centre University of Yaoundé 1 Yaoundé Cameroon Department of Biochemistry Faculty of Science University of Yaoundé 1 Yaoundé Cameroon

Plantain is an important crop that serves as a staple food and contributes significantly to income generation for millions of people in tropical and sub-Saharan Africa. Its cultivation faces the main constraint of seedlings unavailability in quantity and quality, essential for the creation of new plantations. The advent and popularization of the plantlets from stem bits (PIF) in the 2000s raised hopes for solving this problem. However, after about ten years, the PIF has shown some problems limiting its adoption and should be improved for more efficiency. The amendment of PIF substrate production with Tithonia diversifolia could be an alternative to seedlings’ unavailability. This study aims to evaluate the potential stimulative effect of T. diversifolia mulch on plantain PIF seedlings growth and protection against black Sigatoka disease (BSD)caused by Mycosphaerella fijiensis. The parameters of vegetative growth stages and biomarkers accumulation were assessed in sterilized substrate and non-sterilized substrate conditions. T. diversifolia mulch treatment increases the germination rate, the number of shoots, the height and the diameter of shoots, the leaf area as well as the seedlings roots, but it also protects the seedlings against BSD up to about 81% compared to the control seedlings. It also enhances the accumulation of biomarkers such as proteins, polyphenols content and defense-related enzymes (peroxidase, polyphenol oxidase and glucanase). T. diversifolia mulch seems to act in PIF seedlings production as a vital stimulator. It can therefore be taken as a tool for a more sustainable and resilient agriculture, and for poverty alleviation of poor small holder farmers.

关键词： Plantain PIF Seedlings Mulch Tithonia diversifolia Mycosphaerella fijiensis Vital Stimulator

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Plantain Bananas PIF Seedlings Treatment with Liquid extracts of Tithonia diversifoliaInduces Resistance to Black Sigatoka Disease

引用

American Journal of Plant Sciences 2020年第5期11卷 653-671页

作者： Robinson Nembot Tatsegouock Cécile Annie ewané Arouna Meshuneke thaddée boudjeko Laboratory of Phytoprotection and Plant Valorization Biotechnology Center University of Yaoundé 1 Messa-Yaoundé Cameroon Department of Biochemistry Faculty of Science University of Yaoundé 1 Yaoundé Cameroon.

Plantain bananas culture encounters the problems of seedlings quantity and quality unavailability. Black Sigatoka Disease (BSD) is one of the main pathological constraints of banana that can severely reduce the photosynthetic leaf area, leading to the losses of production of about 50% in banana plantation. The use of liquid extracts of Tithonia diversifolia could potentially induced the resistance of the PIF seedlings to BSD during the vegetative stages in the nursery. The aim of this work was to evaluate the effect of Tithonia diversifolia liquid extracts against the development of BSD on the PIF plantain bananas seedlings. The explants in the greenhouse and the seedlings in the shade were watered with liquid extracts of T. diversifolia during the process of PIF seedling production in sterile and non-sterile conditions. The treated seedlings show a more effective enhancement of seedlings quality parameters and also induce resistance against BSD. The vegetative stages parameters (the number of shoots, the diameter and the height of shoots, the foliar area, the length and weight roots) were improved in treated seedlings compared to controls ones. The treated seedlings showed maximum protection against BSD of up to 87% compared to controls. They also exhibited an increase in the accumulation of total proteins and total phenolics, as well as the activity of defense-related enzymes (peroxidase, polyphenol oxidase and glucanase). The treatment seems to acts as a vital stimulator and could therefore be a useful tool for small holder farmers favouring an eco-friendly agriculture using fewer synthetic inputs.

关键词： Plantain Banana PIF Seedlings Tithonia diversifolia Black Sigatoka Disease Induced Resistance Vital Stimulator

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Antioxidant properties of cell wall polysaccharides of Stevia rebaudiana leaves

引用

Journal of Coastal Life Medicine 2014年第12期 962-969页

作者： Mediesse Kengne Francine Woguia Alice Louise Fogue Souopgui Pythagore Atogho-Tiedeu Barbara Simo Gustave thaddée boudjeko Laboratory of Phytoprotection and Valorization of Plants Resources Biotechnology Centre-NkolbissonPo.Box 3851MessaYaoundeCameroon Department of Biochemistry Faculty of ScienceUniversity of Yaounde IPo.Box 812 YaoundeCameroon Department of Biochemistry Faculty of ScienceUniversity of DschangPo.Box 67 DschangCameroon Laboratory for Metabolic and Molecular Medicine Biotechnology Centre-NkolbissonPo.Box 3851MessaYaoundeCameroon

Objective:To examine the total phenolic and protein contents,and the antioxidant activities of cell wall polysaccharide fractions of Stevia rebaudiana ***:Three different polysaccharide-enriched fractions,namely FPe(extract with 50 mmol/L ethylene diamine tetra acetic acid),FPK(extract with 0.05 mol/L KOH)and FH(extract with 4 mol/L KOH)were extracted from Stevia rebaudiana *** antioxidant activity of these fractions was evaluated based on their ability to scavenge DPPH(1,1-diphenyl-2-picryl hydrazyl)free radical,to reduce ferric power,to chelate ferrous ion and to protect human ***:The results indicated that protein content was found to be higher in FPK polysaccharide enriched fraction(47.48μg per mg of FPK).Furthermore,the phenolic compound analysis according to the Folin-Ciocalteu method was higher in FPK(17.71μg ferulic acid).The DPPH maximal inhibition percentage of the three polysaccharide-enriched fractions at 400μg/mL was 27.66%,59.90%and 23.21%respectively for FPe,FPK and *** the polysaccharide fractions exhibited a ferric reducing power except the FH *** three fractions also exhibited lipid peroxidation inhibition,and they completely reverted the DNA damage induced by H_(2)O_(2)/FeCl_(2).FPK showed the strongest scavenging activity against the DPPH radical,the best chelating ability and lipid peroxidation ***:Stevia cell wall polysaccharide fractions are potent protective agents against oxidative *** analysis revealed major differences in the antioxidant activity in the three polysaccharides ***,the 0.05 mol/L KOH pectin fraction(FPK)showed better antioxidant activity.

关键词： Stevia rebaudiana Cell wall polysaccharides Fractions Antioxidant properties

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Potential Biostimulant effect of Clam Shells on Growth Promotion of Plantain PIF Seedlings (var. Big ebanga &Batard) and Relation to Black Sigatoka Disease Susceptibility

引用

American Journal of Plant Sciences 2019年第10期10卷 1763-1788页

作者： Cécile Annie ewane Félix Ndongo Katy Ngoula Paul Martial Tene Tayo Stephen Obol Opiyo thaddée boudjeko Laboratory of Phytoprotection and Plant Valorization Biotechnology Center University of Yaoundé 1 Messa-Yaoundé Cameroon Department of Biochemistry Faculty of Science University of Yaoundé 1 Yaoundé Cameroon Molecular and Cellular Imaging Canter-Columbus OARDC The Ohio State University Columbus OH USA.

Plantain contributes significantly to income generation and food security for millions of people in the world. However, it faces problems of seedlings quantity, quality and availability. The innovation of the “plants issus de fragments de tiges” (PIF) technique could be a solution to these problems for small holders’ farmers. The aim of this research is to evaluate the effect of clam shells through amendment of Batard and Big ebanga PIF substrate, on the growth promotion of seedlings and their protection against black Sigatoka disease (BSD). Plantain PIF seedlings of the two varieties were grown in a substrate amended with 1% concentration of the clam shells powder in the presence of negative control in the sterile and non-sterile conditions. Agromorphological characteristics, susceptibility level to BSD, total proteins and polyphenols content were assessed. Because of the presence of clam shells in the substrates, explants germinated quickly, generated high number of shoots, grew taller by 32%, with a diameter of pseudo stems of 30%, and area of leaves of 18% compared to control. In addition, the seedlings were less susceptible to BSD by 73% compared to those of controls. The treatment seems to allow the accumulation of larger amounts of total proteins and polyphenols before inoculation and after inoculation that could participate in the growth promotion and the reduction of plant’s susceptibility level. Clam shells treatment acts as a biofertilizer/biopesticide and could be helpful to boost production of plantain seedlings, the use of the by-products of fishing in agriculture and helps alleviate poverty of small holders’ farmers.

关键词： Plantain (Musa spp.) PIF Seedlings Clam Shells Biostimulant Potential Biofertilizer Biopesticide Black Sigatoka Disease

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

enhancement of Theobroma cacaoSeedling Growth and Tolerance to Phytophthora megakaryaby Heat-Treated Oyster Shell Powder

引用

American Journal of Plant Sciences 2019年第4期10卷 578-594页

作者： Tene Tayo Paul Martial Dzelamonyuy Aristide Omokolo Ndoumou Denis boudjeko thaddée Laboratory of Phytoprotection and Plant Valorization Biotechnology Center University of Yaoundé I Yaounde Cameroon Department of Biochemistry Faculty of Science University of Yaounde I Yaounde Cameroon Department of Biological Science Laboratory of Plant Physiology Higher Teacher’s Training College Yaounde Cameroon

The aim of this study was to evaluate the ability of oyster shell powder soil amendment to enhance cocoa seedling growth and induce resistance against Phytophthora megakarya in nurseries. The results showed that heat-treated oyster shells powder at 1% (w/w) soil amendment significantly increased plant height, leaf number, leaf area, dry shoot and root weight more than chemical fungicide and control treatment after twelve weeks of growth. The results showed that heat-treated oyster shell powder raised soil pH significantly and reduced P. megakarya load of the soil suspension by 82%. Assessment of resistance stimulation by leaf inoculation showed the highest level of resistance recorded in plants treated either with heat-treated or non-treated oyster shell powder. Furthermore, total phenolic compounds contents, total soluble proteins contents, polyphenoloxidase, chitinase, peroxidase and β-1,3-glucanases activities increased in both healthy or infected leaves from cacao plants treated with oyster shell powder more than those treated with chemical fungicide. These findings demonstrated that heat-treated oyster shell powder could be used as biofertilizer and biofungicide to improve the quality of cocoa seedling production and protect the plant against P. megakarya.

关键词： Cocoa Seedling Phytophthora megakarya Oyster Shell Biofungicide

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Primed expression of Defense-Related Genes by Streptomyces cameroonensis-Based Bioformulation (SCaB) on Cocoa Seedlings in a Nursery Challenged with Phytophthora megakarya

引用

American Journal of Plant Sciences 2023年第12期14卷 1480-1497页

作者： Dzelamonyuy Aristide Téné Tayo Paul Martial Alain Bopda Waffo effa Onomo Pierre ewané Cécile Annie boudjeko thaddée Laboratory of Phytoprotection and Valorization of Genetic Resources Biotechnology Center University of Yaounde I Yaounde Cameroon Department of Biochemistry and Molecular Biology Indiana University School of Medicine Indianapolis IN USA Department of Biochemistry Faculty of Science University of Yaounde 1 Yaounde Cameroon Laboratory of Plant Physiology Department of Biological Sciences Higher Teacher’s Training College Yaounde Cameroon

A Streptomyces cameroonensis based bioformulation (SCaB) has been developed and shown to be stable and effective in controlling the early proliferation of P. megakarya and promoting the growth of cocoa seedlings in nursery. This study was carried out to explore the molecular mechanisms associated with the interaction of SCaB, cocoa seedlings, and the pathogen during the early stages of seedling growth in the nursery. For this purpose, seedling treatment with 10% W/W SCaB under greenhouse conditions evaluated SCaB’s capacity to stimulate the defense mechanisms in cocoa. Agronomic growth parameters and the level of induction of defense-associated compounds were analyzed. Real-time (rt) PCR was used to assess the level of expression of defense genes. Here, we showed that the application of SCaB as a seedling treatment enhanced the growth of cocoa seedlings in the nursery by an average of 15.6% after 30 days of growth and led to an average reduction in disease severity of 64% when challenged with P. megakarya. The latter led to an increased synthesis of total phenolic compounds, flavonoids, chitinases, peroxidases, and β-1,3-glucanases and an induced up-regulation of TcChiB, TcGlu-1, TcPer-1, and TcMYBPA genes. This research provides a basis for the optimization of beneficial microorganisms as a viable alternative to chemical fungicides used in disease suppression.

关键词： Cocoa Seedlings Phytophthora megakarya Streptomyces cameroonensis Bioformulation Priming

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Formulation of Biofungicides from Cymbopogon citratus and Tithonia diversifolia: evaluating Its Antimicrobial Activities against Pythium myriotylum, the Causal Agent of Root Rot of Xanthosoma sagittifolium (L.) Schott

引用

American Journal of Plant Sciences 2023年第8期14卷 896-914页

作者： Magni Pacha Tatiana Flore Tene Tayo Paul Martial Foka Tatiekam ebenezer Dzelamonyuy Aristide ewane Cecile Annie Mbouopda Herman Désire boudjeko thaddée Laboratory of Phytoprotection and Valorization of Genetic Resources Biotechnology Centre-Nkolbisson Yaounde Cameroon Department of Biochemistry and Molecular Biology Indiana University School of Medicine Indianapolis USA Department of Biochemistry University of Yaounde I Yaounde Cameroon Department of Biology University of Bamenda Bamenda Cameroon

Three fungicide formulations, namely M1, M2 and M3, were prepared from sodium bicarbonate, citronella essential oil and sunflower slurry. The stability of M1, M2 and M3 formulations was determined based on pH, temperature, order of incorporation of the inputs and storage time. The most stable formulations were used for antagonistic tests on Pythium myriotylum. The Minimum Inhibitory Concentration (MIC) was used for the greenhouse tests and the mode of action was determined in vitro. The study showed that the order of incorporation of the inputs “essential Oil-Tween 80-Bicarbonate-Slurry” (eO-T80-B-S) promotes stability. M1 and M2 are stable at 4°C, 25°C, 37°C and 40°C temperatures and have a pH of 7 and 8 respectively. The Minimum Inhibitory Concentration of M1 and M2 is 1% on P. myriotylum. M1 and M2 act on Pythium by membrane lysis, inhibiting proton pumps and inhibiting protein synthesis. The formulations M1 and M2 reduce the incidence of root rot disease in cocoyam plants growth in the greenhouse. M1 and M2 are potential candidates for improvement of cocoyam seedlings production in Cameroon.

关键词： Biofungicide Xanthosoma sagittifolium Pythium myriotylum Root Rot Disease

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tithonia diversifoliaLeaves and Stems Use as Substrate Amendment Promote the Growth of Plantain Vivoplants in the Nursery

引用

Agricultural Sciences 2020年第9期11卷 849-859页

作者： C. A. ewané N. T. Mbanya T. boudjeko Laboratory of Phytoprotection and Plant Valorization Biotechnology Center University of Yaoundé 1 Yaoundé Cameroon Department of Biochemistry Faculty of Science University of Yaoundé 1 Yaoundé Cameroon

Tithonia diversifolia is a year-round weed that decomposes rapidly after application as a soil amendment and it is more and more used in agriculture due to its numerous properties. The use of Tithonia diversifolia in ... 详细信息

em>Tithonia diversifoliaem> is a year-round weed that decomposes rapidly after application as a soil amendment and it is more and more used in agriculture due to its numerous properties. The use of em>Tithonia diversifoliaem> in the production of plantain vivoplants is a great opportunity for small holders’ farmers that cannot easily buy chemical inputs for yield improvement. All plant parts are used because of their richness in nutrients, mineral elements and phytochemicals without knowing which part is the most efficient. em>Tithonia diversifoliaem> used as substrate amendment for plantain vivoplants production in nursery could promote their growth. This study aims to evaluate the effect of em>T. diversifoliaem> leaves and stems as substrate amendment on plantain vivoplants growth promotion. The vivoplants were produced in substrates amended with em>T. diversifoliaem> leaves, stems, combination of leaves and stems, and control without amendment. The vegetative growth parameters (number of shoots, height and diameter of shoots, area of shoots leaves) were assessed in sterilized substrate and unsterilized substrate conditions. em>T. diversifoliaem> leaves and stems treatment increases the number of shoots, the height and the diameter of shoots as well as the area of shoots leaves compared to the control. em>T. diversifoliaem> stems are the more efficient treatment, followed by the combination of em>T. diversifoliaem> leaves and stems and finally the em>T. diversifoliaem> leaves. They act as a biofertilizer promoting thus, the growth of plantain vivoplants in the nursery. All parts of em>Tithonia diversifoliaem> can therefore be used by poor small holder farmers, as green manure for soil amendment in a green agriculture context.

关键词： Plantain Vivoplants Tithonia diversifolia Substrate Amendment Growth Promotion Biofertilizer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Primed expression of Defense-Related Genes by Streptomyces cameroonensis -Based Bioformulation (SCaB) on Cocoa Seedlings in a Nursery Challenged with Phytophthora megakarya

引用

American Journal of Plant Sciences（美国植物学期刊） 2023年第12期

作者： Dzelamonyuy Aristide Téné Tayo Paul Martial Alain Bopda Waffo effa Onomo Pierre ewané Cécile Annie boudjeko thaddée

来源：博看期刊评论

在线全文

博看期刊

学校读者我要写书评

暂无评论

Formulation of Biofungicides from Cymbopogon citratus and Tithonia diversifolia : evaluating Its Antimicrobial Activities against Pythium myriotylum, the Causal Agent of Root Rot of Xanthosoma sagittifolium (L.) Schott

引用

American Journal of Plant Sciences（美国植物学期刊） 2023年第8期

作者： Magni Pacha Tatiana Flore Tene Tayo Paul Martial Foka Tatiekam ebenezer Dzelamonyuy Aristide ewane Cecile Annie Mbouopda Herman Désire boudjeko thaddée1

来源：博看期刊评论

在线全文

博看期刊

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：