Formulation of Biofungicides from Cymbopogon citratus and Tithonia diversifolia: Evaluating Its Antimicrobial Activities against Pythium myriotylum, the Causal Agent of Root Rot of Xanthosoma sagittifolium (L.) Schott

作     者：Magni Pacha Tatiana Flore Tene Tayo Paul Martial Foka Tatiekam Ebenezer Dzelamonyuy Aristide Ewane Cecile Annie Mbouopda Herman Désire Boudjeko Thaddée Magni Pacha Tatiana Flore;Tene Tayo Paul Martial;Foka Tatiekam Ebenezer;Dzelamonyuy Aristide;Ewane Cecile Annie;Mbouopda Herman Désire;Boudjeko Thaddée

作者机构：Laboratory of Phytoprotection and Valorization of Genetic Resources Biotechnology Centre-Nkolbisson Yaounde Cameroon Department of Biochemistry and Molecular Biology Indiana University School of Medicine Indianapolis USA Department of Biochemistry University of Yaounde I Yaounde Cameroon Department of Biology University of Bamenda Bamenda Cameroon 

出 版 物：《American Journal of Plant Sciences》 (美国植物学期刊（英文）)

年 卷 期：2023年第14卷第8期

页      面：896-914页

学科分类：09[农学] 0904[农学-植物保护] 

主　　题：Biofungicide Xanthosoma sagittifolium Pythium myriotylum Root Rot Disease 

摘      要：Three fungicide formulations, namely M1, M2 and M3, were prepared from sodium bicarbonate, citronella essential oil and sunflower slurry. The stability of M1, M2 and M3 formulations was determined based on pH, temperature, order of incorporation of the inputs and storage time. The most stable formulations were used for antagonistic tests on Pythium myriotylum. The Minimum Inhibitory Concentration (MIC) was used for the greenhouse tests and the mode of action was determined in vitro. The study showed that the order of incorporation of the inputs “Essential Oil-Tween 80-Bicarbonate-Slurry (EO-T80-B-S) promotes stability. M1 and M2 are stable at 4°C, 25°C, 37°C and 40°C temperatures and have a pH of 7 and 8 respectively. The Minimum Inhibitory Concentration of M1 and M2 is 1% on P. myriotylum. M1 and M2 act on Pythium by membrane lysis, inhibiting proton pumps and inhibiting protein synthesis. The formulations M1 and M2 reduce the incidence of root rot disease in cocoyam plants growth in the greenhouse. M1 and M2 are potential candidates for improvement of cocoyam seedlings production in Cameroon.