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Antimicrobial and synergistic effects of lemongrass and geranium essential oils against Streptococcus mutans,Staphylococcus aureus,and Candida spp.

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World Journal of Critical Care Medicine 2024年第3期13卷 63-74页

作者： Patrícia Michelle Nagai de Lima thaís Cristine Pereira Lara Steffany de Carvalho Letícia Ferreira dos santos Carlos Eduardo Rocha Oliveira Lucas de Paula Ramos Maria Cristina Marcucci Amjad Abu hasna Luciane Dias de Oliveira Department of Biosciences and Oral Diagnosis Institute of Science and TechnologySão Paulo State UniversitySão Josédos Campos 12245000SPBrazil Anhembi Morumbi University School of MedicineSão Josédos Campos 12230-002SPBrazil Department of Restorative Dentistry Endodontics DivisionInstitute of Science and TechnologySão Paulo State UniversitySão Josédos Campos 12245000São PauloBrazil

BACKGROUND The oral cavity harbors more than 700 species of bacteria,which play crucial roles in the development of various oral diseases including caries,endodontic infection,periodontal infection,and diverse oral *** To investigate the antimicrobial action of Cymbopogon Schoenanthus and Pelargonium graveolens essential oils against Streptococcus mutans,Staphylococcus aureus,Candida albicans,***,and *** Minimum microbicidal concentration was determined following Clinical and Laboratory Standards Institute *** synergistic antimicrobial activity was evaluated using the Broth microdilution checkerboard method,and the antibiofilm activity was evaluated with the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide *** were analyzed by one-way analysis of variance followed by the Tukey post-hoc test(P≤0.05).RESULTS *** and *** essential oils were as effective as 0.12%chlorhexidine against *** and *** monotypic biofilms after 24 *** 24 h *** essential oil at 0.25%was more effective than the nystatin group,and *** essential oil at 0.25%was as effective as the nystatin *** *** and *** essential oils are effective against ***,***,***,***,and *** at different concentrations after 5 min and 24 h.

关键词： Antifungal effect Lemongrass Geranium Candida albicans Candida dubliniensis Candida krusei Staphylococcus aureus Streptococcus mutans

Effects of Yerba Mate Consumption (Ilex paraguariensis) on Cardiac Remodeling in Rats

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Food and Nutrition Sciences 2024年第8期15卷 827-845页

作者： Carlos Eduardo Brochini de Paiva thaíssa Baptista Brochini de Paiva Isabelle Tiburcio Pecin Ferreira sandra Cristina Genaro Danilo Silva Martins santos thaoan Bruno Mariano Robson Chacon Castoldi Rafael Stuani Floriano Julia Amanda Rodrigues Fracasso Lucas Pires Guarnier Marina Politi Okoshi Francis Lopes Pacagnelli Postgraduate Program in health Sciences Unoeste Presidente Prudente Brazil Faculty of Medicine Unoeste Presidente Prudente Brazil Department of Physiotherapy Unoeste Presidente Prudente Brazil Department of Nutrition Unoeste Presidente Prudente Brazil Postgraduate Program in Science and Movement Unesp Rio Claro Brazil Postgraduate Program in health Promotion University of Northern Paran (UNOPAR) Londrina Brazil School of Dentistry So Paulo State University (UNESP) Araatuba Brazil Department of Genetics Ribeiro Preto Medical School University of So Paulo USP Ribeiro Preto Brazil Internal Medicine Department Botucatu Medical School So Paulo State University (UNESP) Botucatu Brazil

This study investigated the effects of yerba mate consumption, a South American beverage, on cardiac remodeling in rats. For this purpose, 24 male Wistar rats were divided into Control Group (CG) which received filtered water and a standard diet, and Yerba Mate Group (YM), 6 g of Ilex paraguariensis in 100 ml water and the same diet, for 30 days. The YM group showed a reduction in final body weight and food consumption without altering weight gain. Regarding cardiac remodeling, the YM group exhibited a decrease in the right ventricle weight/final body weight ratio, suggesting cardiac atrophy, without affecting the atria and left ventricle. There was no change in cardiomyocyte area or nuclear fractal dimension in both groups. however, animals that consumed yerba mate showed increased collagen deposition and a smaller fractal dimension in the left ventricle. The consumption of yerba mate at room temperature for 30 days induced changes in cardiac remodeling, as evidenced by increased collagen deposition and alterations in fractal dimension in the left ventricle.

关键词： Tereré Ilex paraguariensis Yerba Mate Cardiac Remodeling Fractal Dimension hypertrophy

Artemisia vulgaris Induces Tumor-Selective Ferroptosis and Necroptosis via Lysosomal Ca^(2+) Signaling

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Chinese Journal of Integrative Medicine 2024年第6期30卷 525-533页

作者： Lucas dos santos Zamarioli Michele Rosana Maia santos Adolfo Garcia Erustes Vanessa Marques Meccatti thais Cristine Pereira Soraya S Smaili Maria Cristina Marcucci Carlos Rocha Oliveira Gustavo JS Pereira Claudia Bincoletto Department of Pharmacology Paulista School of MedicineFederal University of São PauloSão PauloSP04044-020Brazil Research Group on Phytocomplexes and Cell Signaling School of Health SciencesAnhembi Morumbi UniversitySão PauloSP03164-000Brazil Department of Biosciences and Oral Diagnosis Institute of Science and TechnologySão Paulo State University(ICT-UNESP)São PauloSP12231-280Brazil

Objective To evaluate the chemical composition and effects of Artemisia vulgaris(AV)hydroalcoholic extract(hEAV)on breast cancer cells(MCF-7 and SKBR-3),chronic myeloid leukemia(K562)and NIh/3T3 *** Phytochemical analysis of hEAV was done by high-performance liquid chromatography-mass(hPLC)*** and cell death studies were performed using trypan blue and Annexin/FITC-7AAD,***-1(Fer-1)and necrostatin-1(Nec-1)were used to assess the mode of hEAV-induced cell death and acetoxymethylester(BAPTA-AM)was used to verify the involvement of cytosolic calcium in this *** calcium measurements were made using *** hEAV decreased the viability of MCF-7,SKBR-3 and K562 cells(Phe viability of hEAV-treated K562 cells was reduced compared to hEAV-exposed fibroblasts(Ph hEAV induced cell death primarily by late apoptosis and necrosis in assays using annexin V-FITC/7-AAD(Phe use of Nec-1 and Fer-1 increased the viability of K562 cells treated with hEAV relative to cells exposed to hEAV alone(PhEAV-induced Ca^(2+)release mainly from lysosomes in K562 cells(Phermore,BAPTA-AM,an intracellular Ca^(2+)chelator,decreased the number of non-viable cells treated with hEAV(PhEAV is cytotoxic and activates several modalities of cell death,which are partially dependent on lysosomal release of Ca^(2+).These effects may be related to artemisinin and caffeoylquinic acids,the main compounds identified in hEAV.

关键词： Artemisia vulgaris necroptosis ferroptosis late apoptosis Ca^(2+)signaling

The versatility of mesenchymal stem cells:From regenerative medicine to COVID,what is next?

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BIOCELL 2022年第4期46卷 913-922页

作者： thaÍS CAsaGRANDE PAIM MÁRCIA ROSÂNGELA WINK Laboratório de Biologia Celular Universidade Federal de Ciências da Saúde de Porto Alegre(UFCSPA)Porto Alegre90050-170Brazil

Mesenchymal stem cells(MSCs)play key roles in regenerative medicine by promoting tissue *** can be isolated from different adult tissues and they are able to differentiate into several *** to their antiinflammatory,angiogenic and immune-modulatory properties,MSCs are suitable for tissue engineering applications and,when associated with biomaterials,their benefits can be ***,recently,MSCs have been studied for new clinical applications,such as in the treatment of patients with *** regenerative potential has been attributed to their secretome,which comprises extracellular matrix,soluble proteins and several elements,including the release of extracellular *** though,in order to explore all their therapeutic potential,it is still necessary to advance in the investigation of their basic cell biology characteristics.

关键词： Mesenchymal stem cells Regenerative medicine Secretome Biomaterials Extracellular vesicles

Dynamical Analysis of DC Shunt Motor Powered by PV Generator Using Perturbation and Observation as MPPT Technique

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Energy and Power Engineering 2017年第1期9卷 55-69页

作者： tha’er Omar Sweidan Electrical Engineering Department The Hashemite University Zarqa Jordan

This paper presents the perturbation and observation (P & O) algorithm as maximum power point tracking (MPPT) method for the dynamical analysis of DC shunt motor fed by photovoltaic generator at different solar irradiance levels. At each solar intensity, the maximum power point of current/voltage (I/V) characteristic of the PV generator is achieved by perturbation and observation algorithm. The nonlinear behavior of (I/V) characteristics of the PV generator at various solar intensities and the magnetization curve of the ferromagnetic material of the DC shunt motor are described by high order polynomial mathematical expression. The dynamical analysis of the DC shunt motor fed by PV generator at different solar intensity has been carried out, also the dynamical analysis of DC shunt motor is investigated at a step change in solar intensity levels with fixed load at motor.

关键词： DC Shunt Motor MPPT P & O PV Generator Dynamical Analysis

Detection and determination of undeclared synthetic caffeine in weight loss formulations using hPLC-DAD and UhPLC-MS/MS

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Journal of Pharmaceutical Analysis 2018年第6期8卷 366-372页

作者： Carine Viana Gabriela M.Zemolin thaís R.Dal Molin Luciana Gobo sandra Maria Ribeiro Gabriela C.Leal Gabriela Z.Marcon Leandro M.de Carvalho Graduate Program in Pharmaceutical Sciences Federal University of Santa Maria (UFSM) Center of health Sciences Federal University of Santa Maria (UFSM) Department of Chemistry Federal University of Santa Maria (UFSM)

Caffeine is present in products marketed for weight loss, with the purpose of increasing thermogenesis and lipid metabolism. The dosage declared by the product manufacturer, or even its presence, is not always correctly described on the label. This work aimed to investigate the undeclared synthetic caffeine in weight loss formulations by a high-performance liquid chromatography with diode array detection(hPLC-DAD) method. From one hundred products purchased through Brazilian e-commerce, seventeen contained caffeine, either naturally or synthetically added to formulation. The caffeine-containing samples were confirmed by an ultra-high performance liquid chromatography-tandem mass spectrometry(UhPLC-MS/MS) method, and adulteration was clearly proven in five products. The content highest caffeine contained 448.8 mg per dose. Other irregularities were also found; nevertheless, the most serious was the addition of synthetic drugs without asking the consumers. Additional drugs expose the consumer to more possible side-effects as well as deleterious drug interactions. Intentional adulteration with any unlabeled substance is typically motivated by a desire to increase or alter the claimed effect of the marketed product to gain a commercial advantage.

关键词： Undeclared synthetic caffeine Weight loss formulations hPLC-DAD UhPLC-MS/MS

Evaluation of Collection and Distribution of samples for histological,Stereological Analysis and Cell Culture of Canine Mast Cell Tumors

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Open Journal of Veterinary Medicine 2012年第4期2卷 216-224页

作者： samanta Rios Melo thaís Andrade Costa-Casagrande Julia Maria Matera Department of Surgery School of Veterinary MedicineUniversity of Sao PauloSao PauloBrazil

Objective: To collect high quality, representative tissue material from tumors and manage its distribution to different laboratories. Design: Prospective controlled study. Animals: Thirty-six dogs with mast cell tumors. Procedures: The samples were submitted for the following analyses: stereology;histopathology;cell culture;breakdown for cytogenetic analysis of chromosomes (based on the Boxer breed published genome);Cell lysis for Real Time PCR and quantification of gene expression of CX 43, 32 and E-cadherin in canine mast cells. Results: Cytogenetic chromosome analysis, 90.9% of the samples were considered to be of good quality. For gene expression quantification of CX 43, 32 and E-cadherin in canine mast cell tumors (MCT), 95.5% of samples were considered to be of good quality. Conclusions and Clinical Relevance: We seek to assess the importance of surgical collection and post-surgical tissue preparation on laboratory testing by collecting surgical material appropriately to allow accurate diagnosis and reliable clinical prognosis and minimize errors caused by inadequate preparation of samples.

关键词： Mast Cell Tumor Oncologic Surgery Canine Tumor Collection Stereological Analysis

Medium Term Load Forecasting for Jordan Electric Power System Using Particle Swarm Optimization Algorithm Based on Least Square Regression Methods

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Journal of Power and Energy Engineering 2017年第2期5卷 75-96页

作者： Mohammed hattab Mohammed Ma’itah tha’er Sweidan Mohammed Rifai Mohammad Momani Electrical Power Engineering Department Yarmouk University Irbid Jordan Electrical Engineering Department The Hashemite University Zarqa Jordan

This paper presents a technique for Medium Term Load Forecasting (MTLF) using Particle Swarm Optimization (PSO) algorithm based on Least Squares Regression Methods to forecast the electric loads of the Jordanian grid for year of 2015. Linear, quadratic and exponential forecast models have been examined to perform this study and compared with the Auto Regressive (AR) model. MTLF models were influenced by the weather which should be considered when predicting the future peak load demand in terms of months and weeks. The main contribution for this paper is the conduction of MTLF study for Jordan on weekly and monthly basis using real data obtained from National Electric Power Company NEPCO. This study is aimed to develop practical models and algorithm techniques for MTLF to be used by the operators of Jordan power grid. The results are compared with the actual peak load data to attain minimum percentage error. The value of the forecasted weekly and monthly peak loads obtained from these models is examined using Least Square Error (LSE). Actual reported data from NEPCO are used to analyze the performance of the proposed approach and the results are reported and compared with the results obtained from PSO algorithm and AR model.

关键词： Medium Term Load Forecasting Particle Swarm Optimization Least Square Regression Methods

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咖啡园的混作

热带农业科技 1986年第4期 44-46页

作者： h.haNUMAN tha RAO,郭亚钢印度

混作的表述很多,如多样化栽培、群作、间作或多层栽培,就是意味着在同一块土地栽培两种和两种以上的一年生作物。从技术上讲,多样化栽培表明与原来种植一种作物的方法不同,而要使其多样化。群作是把两种或两种以上的多年生作物同时种植... 详细信息

混作的表述很多,如多样化栽培、群作、间作或多层栽培,就是意味着在同一块土地栽培两种和两种以上的一年生作物。从技术上讲,多样化栽培表明与原来种植一种作物的方法不同,而要使其多样化。群作是把两种或两种以上的多年生作物同时种植在同一环境里。间作是在长期作物中间栽培一些短期作物。而多层次栽培,就是在同一土地上栽培不同的作物,以最好地利用土地,获得最好的经济产品。总之,所有上述的耕作方法,都是为了利用好现有的土地和资源,使种植者获得最好的经济收益。

关键词：咖啡产量收入树种胡椒财政管理胡椒属经济作物工业原料作物柑桔水果耕作制度混作混种