Evaluation of Collection and Distribution of Samples for Histological,Stereological Analysis and Cell Culture of Canine Mast Cell Tumors

作     者：Samanta Rios Melo Thaís Andrade Costa-Casagrande Julia Maria Matera 

作者机构：Department of SurgerySchool of Veterinary MedicineUniversity of Sao PauloSao PauloBrazil 

出 版 物：《Open Journal of Veterinary Medicine》 (兽医学（英文）)

年 卷 期：2012年第2卷第4期

页      面：216-224页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo(FAPESP) 

主　　题：Mast Cell Tumor Oncologic Surgery Canine Tumor Collection Stereological Analysis 

摘      要：Objective: To collect high quality, representative tissue material from tumors and manage its distribution to different laboratories. Design: Prospective controlled study. Animals: Thirty-six dogs with mast cell tumors. Procedures: The samples were submitted for the following analyses: stereology;histopathology;cell culture;breakdown for cytogenetic analysis of chromosomes (based on the Boxer breed published genome);Cell lysis for Real Time PCR and quantification of gene expression of CX 43, 32 and E-cadherin in canine mast cells. Results: Cytogenetic chromosome analysis, 90.9% of the samples were considered to be of good quality. For gene expression quantification of CX 43, 32 and E-cadherin in canine mast cell tumors (MCT), 95.5% of samples were considered to be of good quality. Conclusions and Clinical Relevance: We seek to assess the importance of surgical collection and post-surgical tissue preparation on laboratory testing by collecting surgical material appropriately to allow accurate diagnosis and reliable clinical prognosis and minimize errors caused by inadequate preparation of samples.