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Lovastatin as a supplement to mitigate rumen methanogenesis:an overview

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Journal of Animal Science and Biotechnology 2022年第2期13卷 353-364页

作者： Amaury Ábrego-Gacía Héctor M.Poggi-Varaldo Vania Robles-González teresa Ponce-Noyola Graciano Calva-Calva Elvira Ríos-Leal Daniel Estrada-Bárcenas Alfredo Mendoza-vargas Department of Biotechnology and Bioengineering CINVESTAV-IPNP.O.Box 17-74007000 Mexico CityMexico Environmental Biotechnology and Renewable Energies Group CINVESTAV-IPNP.O.Box 17-74007000 Mexico CityMexico Instituto de Hidrología Universidad Tecnológica de la MixtecaOaxaca 69000 Huajuapan de LeónMexico National Collection of Microbial and Cellular Cultures CINVESTAV-IPNP.O.Box17-74007000 Mexico CityMexico Unidad de Secuenciación e Identificación de Polimorfismos Instituto Nacional de Medicina Genómica14610 Mexico CityMexico

Methane from enteric fermentation is the gas with the greatest environmental impact emitted by ***(Lv)addition to feedstocks could be a strategy to mitigate rumen methane emissions via decreasing the population of methanogenic archaea(MA).Thus,this paper provides the first overview of the effects of Lv supplementation,focusing on the inhibition of methane production,rumen microbiota,and ruminal *** indicated that Lv treatment had a strong anti-methanogenic effect on pure strains of ***,there are uncertainties from in vitro rumen fermentation trials with complex substrates and rumen ***-state fermentation(SSF)has emerged as a cost-effective option to produce *** this way,SSF of agricultural residues as an Lv-carrier supplement in sheep and goats demonstrated a consistent decrease in ruminal methane *** experimental evidence for in vitro conditions showed that Lv did not affect the volatile fatty acids(VFA).However,in vivo experiments demonstrated that the production of VFA was *** did not negatively affect the digestibility of dry matter during in vitro and in vivo methods,and there is even evidence that it can induce an increase in *** the rumen microbiota,populations of MA were reduced,and no differences were detected in alpha and beta diversity associated with Lv ***,some changes in the relative abundance of the microbiota were *** studies are recommended on:(i)Lv biodegradation products and stability,as well as its adsorption onto the solid matter in the rumen,to gain more insight on the“available”or effective Lv concentration;and(ii)to determine whether the effect of Lv on ruminal fermentation also depends on the feed composition and different ruminants.

关键词： Fermentation Lovastatin Methanogenesis Microbiota Rumen

ACE Score Identifies HBeAg-negative Inactive Carriers at a Single-point Evaluation,Regardless of HBV Genotype

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Journal of Clinical and Translational Hepatology 2022年第6期10卷 1068-1076页

作者： Luisa Roade Mar Riveiro-Barciela Adriana Palom Francisco Rodríguez-Frías Marta Bes Ariadna Rando María teresa Salcedo Rosario Casillas Elena vargas-Accarino David Tabernero Silvia Sauleda Rafael Esteban María Buti Liver Unit Internal Medicine DepartmentHospital Universitari Vall d’HebronVall d’Hebron Barcelona Hospital CampusBarcelonaSpain Universitat Autònoma de Barcelona(UAB) Department of MedicineBarcelonaSpain Universitat Au-tònoma de Barcelona(UAB) Department of Biochemistry and Molecular BiologyBarcelonaSpain Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas(CIBERehd) Instituto de Salud Carlos IIIMadridSpain Liver Pathology Lab Biochemistry and Microbiology Departments(Clinical Laboratories)Hospital Universitari Vall d’HebronVall d’Hebron Barcelona Hospital CampusBarcelonaSpain Vall d’Hebron Institut de Recerca(VHIR) Liver Diseases GroupBarcelonaSpain Transfusion Safety Laboratory Banc de Sang i TeixitsServei Catalàde la SalutBarcelonaSpain De-partment of Pathology Hospital Universitari Vall d’HebronUniversitat Autònoma de BarcelonaBarcelonaSpain

Background and Aims:Hepatitis B virus(HBV)biomark-ers have been used for a better categorization of patients,even though the lack of simple algorithms and the impact of genotypes limit their *** aim was to assess the usefulness of noninvasive markers for the identification of HBV inactive carriers(ICs)in a single-point evaluation and to design a predictive model for their ***-ods:This retrospective-prospective study included 343 consecutive HBeAg-negative ***,analytical,and virological data were collected,and a liver biopsy was performed if *** were classified at the end of follow-up as ICs,chronic hepatitis B and gray zone.A pre-dictive model was constructed,and validated by 1000-boot-strap ***:After 39 months of follow-up,298 subjects were ICs,36 were chronic hepatitis B CHB,and nine were gray ***-nine(25.9%)individuals re-quired a liver *** HBV DNA hazard ratio(HR)6.0,p<0.001),HBV core-related antigen(HBcrAg)(HR 6.5,p<0.001),and elastography(HR 4.6,p<0.001)were inde-pendently associated with the IC *** ACE score(HBV DNA,HBcrAg,elastography),obtained by bootstrapping,yielded an area under the receiver operating characteris-tics(AUROC)of 0.925(95%CI:0.880-0.970,p<0.001)for identification of *** AUROC for genotype D was 0.95,0.96 for A,0.90 for E,and 0.88 for H/*** ACE score of<1 had a positive predictive value of 99.5%,and a score≤12 points had a diagnostic accuracy of 93.8%.Conclusions:Low baseline HBV DNA,HBcrAg,and liver stiffness were in-dependently associated with the IC phase.A score including those variables identified ICs at a single-point evaluation,and might be applied to implement less intensive follow-up strategies.

关键词： Hepatitis B virus Inactive carrier Liver stiffness HBV DNA Quan-titative HBsAg Core-related antigen.

Identification and Quantification of Monosaccharides in Aloe vera Gel by Acid and Enzymatic Hydrolysis and Wet Heat Treatment

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Food and Nutrition Sciences 2017年第8期8卷 840-853页

作者： Dianeli Madera Pina Carlos Hernán Herrera Méndez Lourdes vargas y vargas Herbert Loria Sunza Jorge Tamayo cortez Research and Postgraduate Division Instituto Tecnológico de MéridaMéridaMexico Agroindustrial Engineering Department Campus Celaya-SalvatierraUniversidad de GuanajuatoSalvatierraMéxico

Aloe vera (Aloe barbadensis Miller) possesses curative and therapeutic properties attributed to the polysaccharides found in its tissue. This work aimed to study hydrolyzed (chemical and enzymatical) Aloe vera gel extracts using ultrafiltration membranes with a pore size of 0.22 micron (μm). Nine chemical treatments were achieved with H2SO4 and three wet heat treatments were carried out at a pressure of 1.2 lbf/in2 for 15 minutes;in both cases, three different concentrations of Aloe vera gel juice (AGJ) were used: 1.5%, 2.5% and 3.5% w/w. The concentrations of H2SO4 were 0.10%, 0.25% and 0.40% w/w. Chemical experiments are performed over a factorial 32 design and results were analyzed using SPSS software (version 17, SPSS Inc.), finding the one labeled T7 (15 ml of AGJ 3.5% with 15 ml of H2SO4 0.10% added) the best of them, as it leaded to 0.0446 mg/ml of liberated glucose. Among the three wet heat treatments, the one labeled TC3 (15 ml of AGJ 3.5% with 15 ml of H2O added) was the best-performing one, as it leaded to 0.292 mg/ml of liberated glucose. Furthermore, an enzymatic hydrolysis was carried out using Novozymes’ Pectinex? AR and Viscozyme?. Hydrolisis with both enzymes yield to better results than acid hydrolysis: in the treatment with Pectinex? AR, 3.282 mg/ml of liberated glucose were obtained and 3.302 mg/ml in the treatment with Viscozyme?. The hydrolyzed substances obtained by acid and enzymatic hydrolysis, as well as by wet heat treatment, were subsequently analyzed by thin layer chromatography (TLC), using glucose, galactose and arabinose 1000 ppm solutions as reference patterns. Among the treatments by H2SO4, the one labeled T4 obtained an Rf value of 50, the same as on the galactose reference pattern.

关键词： Carbohydrates Enzyme Mucilage Reducing Sugars Retention Factor

Purification and Partial Characterization of Polyphenol Oxidase from Sapodilla Plum (<i>Achras sapota</i>)

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Food and Nutrition Sciences 2013年第7期4卷 727-734页

作者： Jorge Tamayo cortez Carlos Hernán Herrera Méndez Enrique Sauri Duch María de Lourdes vargas y vargas Sara Solís Pereira Agroindustrial Engineering Department Campus Celaya-Salvatierra Universidad de Guanajuato Salvatierra Mexico. Research and Postgraduate Division Instituto Tecnológico de Mérida Merida Mexico

The browning of fruits can be considered as an enzymatic oxidation which is believed to be one of the main causes of quality loss during post-harvest handling. The enzymes responsible for this are the oxidoreductases;the polyphenol oxidase (PPO) (monophenol, o-diphenol, oxygen oxidoreductase;EC 1.***.18.1) belongs to this group. This enzyme, which is found in the sapodilla plum (Achras sapota), was purified using a phenylsepharose and a SephacrylS-200 columns. The molecular weight of the purified enzyme was estimated to be about 66 kDa by gel filtration and 29 kDa by SDS-PAGE. A single protein band was found using the latter system (SDS-PAGE), which shows that the PPO of the pulp of the sapodilla plum may be composed of two protein subunits with similar molecular weight. The optimum pH was 7.0 and the optimum temperature 60℃. The most effective inhibitors tested were ascorbic acid, sodium metabisulfite and acetic acid.

关键词： Enzymatic System Oxidation Polyphenol Oxidase Browning Process Nutritional Value

Main Polysaccharides Isolated and Quantified of Aloe vera Gel in Different Seasons of the Year

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Food and Nutrition Sciences 2016年第6期7卷 447-453页

作者： Berenice Aranda Cuevas Carlos Hernán Herrera Méndez Ignacio Islas Flores Sara Solís-Pereira Luis Cuevas-Glory Gerardo Rivera Muñoz María de Lourdes vargas y vargas Jorge Tamayo cortez Research and Postgraduate Division Instituto Tecnológico de Mérida Mérida Mexico Agroindustrial Engineering Department Universidad de Guanajuato Campus Celaya-Salvatierra Salvatierra México Centro de Investigación Científica de Yucatán Mérida México

We developed and implemented a methodology that allowed extracting and evaluating high molecular weight polysaccharides present in the gel of Aloe barbadensis Miller. One of the fractions evaluated revealed the presence of high molecular weight carbohydrates (200 kDa) with a behavior similar to that of acemannan and another fraction with compounds of molecular weights between 17 and 47 kDa. We quantified the concentration of acemannan for two different growing periods. The concentration of acemannan in the high molecular weight fraction was 99.97 ppm in the rainy season and 106.03 ppm in the dry season. The concentration of acemannan in the fraction of low molecular weight was 9.364 ppm during the season of greatest rainfall and 26.939 ppm in the dry season.

关键词： Acemannan Aloe vera Gel Polysaccharides Rainy Season Dry Season

Extraction and Stability of Anthocyanins Present in the Skin of the Dragon Fruit (<i>Hylocereus undatus</i>)

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Food and Nutrition Sciences 2013年第12期4卷 1221-1228页

作者： María de Lourdes vargasy vargas Jorge Abraham Tamayo cortez Enrique Sauri Duch Andrés Pech Lizama Carlos Hernán Herrera Méndez Agroindustrial Engineering Department Campus Celaya-Salvatierra Universidad de Guanajuato Salvatierra México Research and Postgraduate Division Instituto Tecnológico de Mérida Mérida Mexico

The extraction of anthocyanins present in the skin of the dragon fruit was performed using trifluoroacetic acid (TFA) plus a mixture of methanol, acetic acid and water;the anthocyanins were then purified with a LC-18 cartridge, using methanol acidified with TFA as eluent, reaching concentrations of 44.3865 ± 1.3125 mg/100g of sample. The extracts were put through stability tests under different storage conditions, modifying the pH of the extracts (pH of 1, 4 and 6), the temperature (4℃, 25℃ and 68℃) and the absence and presence of light for a time period of 4 days;the tests indiated that anthocyanins remain more stable at a temperature of 4℃?with a pH of 4 in the absence of light, retaining up to 80% of the pigment. Three anthocyanins were partially identified in the extracts by high performance liquid chromatography (HPLC);they were: cyanidin 3-O-glucoside, cyanidin 3,5 O-glucoside and pelargonidin 3,5 O-glucoside.

关键词： Anthocyanins Dragon Fruit Stability

Nutritional Situation of Rural Communities in Narino,Colombia 2013

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Food and Nutrition Sciences 2014年第15期5卷 1521-1528页

作者： Sara Eloísa Del Castillo Matamoros teresa Mosquera Vásquez Eliana Lorena Suárez Higuera Ana Patricia Heredia vargas Department of Human Nutrition National University of ColombiaBogotáColombia Food and Nutrition Security Observatory(OBSAN) National University of ColombiaBogotáColombia Faculty of Agricultural Sciences National University of ColombiaBogotáColombia

Objective: To evaluate the nutritional situation by anthropometry of the rural families population aged 0 to 64 years in the municipalities of Pasto, Túquerres, Guachucal, Cumbal and Cuaspud in the Nari?o Department (Colombia). Materials and Methods: As part of the research “Improving potato production as a food security contribution of native communities in Nariño, Colombia”, aged October and December in 2013, an initial diagnosis of the food security situation in farmer families was conducted, through direct visits to the households selected in the sample, where in the household members, after standardization of field team, the anthropometric measures of weight and height were taken, using electronic scales, infantometers and stadiometers. Conclusions: A significant stunting was reported in children up to 7 years. In children aged 0 - 5 years group and children aged 5 - 17 years group, the results exceed by more than double the department data (according to National Survey of the Nutritional Situation in Colombia ENSIN 2010), and almost three times the national data. The underweight was generally low in all the analysed groups, although concerned the significant overweight percentage. In children aged 0 - 5 years group, the overweight data (above 2 SD) exceeded twice the department and national data according to the ENSIN 2010, although in the children aged 5 - 17 years group, the overweight and obesity was lower than the departmental and national data. In adults, the results reported that one of each two people in the range of 18 to 64 years in the five municipalities had excess weight (overweight or obesity) which was similar data respected to the department and national data. Additionally, it was noted that 18% of households were found among adults with overweight, and simultaneously, children with stunting, this situation has been called double burden of malnutrition and this is an aspect that must be analysed to identify intervention strategies.

关键词： Nutritional Situation Anthropometry Stunting Excess Weight Narino’s Rural Families

对高水平乒乓球运动员腿部爆发力和弹跳力的评价(英文)

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体育科研 2005年第3期26卷 80-85页

作者： Francisco Pradas Carlos de teresa Maricarmen vargas University of Granada & Spanish Table Tennis Association. Spain Andalusian Center of Sport Medicine. St. John of God Hospital. Granada. Spain.Andalusian Center of Sport Medicine. St. John of God Hospital. Granada. Spain.

乒乓球运动是一项非周期性运动,对抗过程中涉及极其复杂的战术、心理和生理因素,球员面对对手的施压需要做出快速反应,其中速度和爆发力是基本的、必备的素质。研究目的:探讨乒乓球运动员腿部爆发力的重要作用。研究方法:本研究选用29... 详细信息

乒乓球运动是一项非周期性运动,对抗过程中涉及极其复杂的战术、心理和生理因素,球员面对对手的施压需要做出快速反应,其中速度和爆发力是基本的、必备的素质。研究目的:探讨乒乓球运动员腿部爆发力的重要作用。研究方法:本研究选用29名优秀运动员为实验对象,其中包括17名男性(年龄23.5±5.5 岁)和12位女性(年龄20.8±2.9岁),均为西班牙国家乒乓球队成员。采用测力台(Ergotester Globus)、Acer笔记本电脑和一些登记册对运动员进行了全蹲跳(CMJ)和半蹲跳(SJ)测试,并对腿部爆发力和弹跳力进行了评价。实验数据的统计处理由SPSS v12.0 和Excel v10.2软件完成。研究结果: 在腿部爆发力和弹跳力方面,男女组存在显著性差异(p<0.001),男运动员优于女性运动员。同样,不同打法的选手之间也存在差异,防守型打法的选手都低于进攻型选手。讨论和结论:在CMJ和SJ测试中,防守型打法的选手腿部爆发力和弹跳力均低于进攻型选手。在实验中成绩较好的男女选手,排名均在西班牙前十二以内,进一步证实了腿部爆发力在此项运动中的重要性,尤其对进攻型打法的选手。其原因可归结为血液中所含睾丸激素水平的高低,男选手高于女选手,进攻型选手高于防守型。

关键词：乒乓球运动运动员腿部爆发力弹跳力性别打法

小分子金属结合蛋白标记的重组蛋白在大肠杆菌中的表达与纯化

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中国医药工业杂志 2016年第5期47卷 574-574页

作者： vargas-cortez T 石丽娟

使用来源于欧洲亚硝化单胞菌Nitrosomonaseuropaea中的小分子金属结合蛋白（SmbP）作为新的融合蛋白，在大肠杆菌中表达并纯化目标蛋白。使用融合蛋白可以提高外源蛋白的溶解度，同时有利于亲和色谱纯化。

关键词：金属结合蛋白大肠杆菌重组蛋白小分子纯化标记亚硝化单胞菌融合蛋白