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The Effects of Antibiotics, Metals, and Biotic Interactions on the Assembly of Taxonomically Diverse Single and Mixed Species Biofilms

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Advances in Microbiology 2020年第12期10卷 623-646页

作者： Roshan Angoshtari Kim T. Scribner terence l. marsh Department of Microbiology and Molecular Genetics Michigan State University East Lansing MI USA Department of Fisheries and Wildlife Michigan State University East Lansing MI USA

To better understand the assembly of the sturgeon egg microbiome, we purified six bacterial isolates from eggs and characterized their ability to form biofilms under the stress of tobramycin, with and without exogenous protein. In experiments with single species biofilms, tobramycin reduced the metabolic activity of all isolates and increased biofilm biomass of three. The addition of exogenous protein to the assay countered the inhibition of biofilm and metabolic activity by tobramycin of Pseudomonas sp., Brevundimonas sp., Flavobacterium columnare and mixed biofilms of Pseudomonas-F. columnare and Brevundimonas-Hydrogenophaga. Two of the isolates (Pseudomonas spp.) that produced antimicrobial activity, were effective at reducing biofilm formation by Brevundimonas, but enhanced biofilm formation in other isolates. Increasing concentrations of Mg2+ had no effect on biofilm formation but Ca2+ enhanced biofilm formation of Pseudomonas aeruginosa PA01 (positive control) and Brevundimonas. Biofilm assembly by these two bacteria was inhibited by low concentrations of Ni2+. Mixed biofilms of Brevundimonas and Hydrogenophage consistently produced more robust biofilm than the strains in isolation, suggesting synergism. Established Brevundimonas biofilm appeared adept at recruiting pelagic Acidovorax and Hydrogenophaga into biofilm, suggesting that it plays an important role in the selection of species into the microbiome.

关键词： Biofilm Assembly Antimicrobial Resistance Tobramycin Metabolic Activity Metal Resistance

Derived, still living cockroach genus Cariblattoides （Blattida： Blattellidae） from the Eocene sediments of Green River in Colorado, USA

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Insect Science 2012年第2期19卷 143-152页

作者： Peter Vrsansky l＇ubomir Vidlicka Fedor Ciampor Jr Finnegan marsh Geological Institute SlovakAcademy of Sciences BraUslava Slovakia Paleontological Institute Russian Academy of Sciences Moscow Russia Institute of Zoology Slovak Academy of Sciences Department of Biology Faculty of Education Comenius University Bratislava Slovakia Department of Paleobiology National Museum of Natural History Smithsonian Institution Washington DC USA

Cariblattoides labandeirai sp.n. from the Eocene sediments of Green River in Colorado, USA bear only two plesiomorphies, but also several significant autapomorphies within the advanced and highly derived living cockroach genus. Thus, Cariblattoides with extant occurrence in the Caribbean and South America was historically common in the Nearctic, and represents important evidence for the occurrence of derived living genera of cockroaches -50 Ma ago. Generally, the vast majority of living genera were absent during the Palaeocene, thus the diversification of most living cockroach lineages near the Palaeocene/Eocene boundary must have been extremely rapid. Females of living C. suave, the type species, have identical （sophisticated） coloration ofpronotum, but the most related living taxa are C. piraiensis and *** from Brazil （supported by phylogenetical analysis）.

关键词： Blattida -- Blattaria = Blattodea Cariblattoides Eocene fossil insects,Green River Tertiary cockroaches

Microbial communities biostimulated by ethanol during uranium （VI） bioremediation in contaminated sediment as shown by stable isotope probing

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Frontiers of Environmental Science & Engineering 2015年第3期9卷 453-464页

作者： Mary Beth lEIGH Wei-Min WU Erick CARDENAS Ondrej UHlIK Sue CARROll Terry GENTRY terence l. marsh Jizhong ZHOU Philip JARDINE Craig S. CRIDDlE James M. TIEDJE Center for Microbial Ecology Michigan State University East Lansing MI 48824 USA Institute of Arctic Biology University of Alaska Fairbanks Fairbanks AK 99775 USA Department of Civil and Environmental Engineering Center for Sustainable Development & Global Competitiveness Codiga Resource Recovery Center Stanford University Stanford CA 94305-4020 USA Department of Biochemistry and Microbiology Institute of Chemical Technology Prague Technicka 3 166 28 Prague Czech Republic Environmental Sciences Division Oak Ridge National Laboratory Oak Ridge TN 37831 USA Department of Botany and Microbiology University of Oklahoma Norman OK 73019 USA Department of Crop and Soil Sciences Texas A&M University College Station TX 77843 USA

Stable isotope probing （SIP） was used to identify microbes stimulated by ethanol addition in microcosms containing two sediments collected from the bioremediation test zone at the US Department of Energy Oak Ridge site, TN, USA. One sample was highly bioreduced with ethanol while another was less reduced. Microcosms with the respective sediments were amended with ^13C labeled ethanol and incubated for 7 days for SIP. Ethanol was rapidly converted to acetate within 24h accompanied with the reduction of nitrate and sulfate. The accumulation of acetate persisted beyond the 7 d period. Aqueous U did not decline in the microcosm with the reduced sediment due to desorption of U but continuously declined in the less reduced sample. Microbial growth and concomitant 13C-DNA production was detected when ethanol was exhausted and abundant acetate had accumulated in both microcosms. This coincided with U（VI） reduction in the less reduced sample. I3C originating from ethanol was ultimately utilized for growth, either directly or indirectly, by the dominant microbial community members within 7 days of incubation. The microbial community was comprised predominantly of known denitrifiers, sulfate-reducing bacteria and iron （Ⅲ） reducing bacteria including Desulfovibrio, Sphingomonas, Ferribacterium, Rhodanobacter, Geothrix, Thiobacillus and others, including the known U（VI）-redueing bacteria Acidovorax, Anaeromyxobacter, Desulfovibrio, Geobac- ter and Desulfosporosinus. The findings suggest that ethanol biostimulates the U（VI）-reducing microbial com- munity by first serving as an electron donor for nitrate, sulfate, iron （IH） and U（VI） reduction, and acetate which then functions as electron donor for U（VI） reduction and carbon source for microbial growth.

关键词： Stable isotope probing (S/P) ethanol acetate uranium reduction sediment bioremediation

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Phylogenetic Analysis of Anaerobic Co-Digestion of Animal Manure and Corn Stover Reveals linkages between Bacterial Communities and Digestion Performance

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Advances in Microbiology 2016年第12期6卷 879-897页

作者： Fan Yang Rui Chen Zhengbo Yue Wei liao terence l. marsh Department of Agricultural and Biosystems Engineering Iowa State University Ames IA USA Department of Biosystems and Agricultural Engineering Michigan State University East Lansing MI USA School of Resources and Environmental Engineering Hefei University of Technology Hefei China Department of Microbiology and Molecular Genetics Michigan State University East Lansing MI USA

Over 3 million tons of manures are produced annually in the United States and pose environmental and health risks if not remediated. Anaerobic digestion is an effective method in treating organic wastes to reduce environmental impacts and produce methane as an alternative energy. Previous studies suggested that optimization of feed composition, hydraulic retention time, and other operational conditions can greatly improve total solids removal and increase methane productivity. These environmental factors improve functionality by altering the microbial community structure but explicit details of how the bacterial community shifts are poorly understood. Our investigations were conducted to investigate the relationship between environmental factors, microbial community structure and bioreactor efficiency by using metagenomic analysis of the microbial communities. Our results indicated that the bioreactor with the greatest methane production, digestion efficiency and reduced levels of E. coli/Shigella had a distinctive community structure at the genus level with unique and abundant uncultivated strains of Bacteroidetes. Moreover the same bioreactor was enriched in Aminomonas paucivorans and Clostridia populations that can utilize secondary metabolites produced during cellulose/hemicellulose degradation to generate hydrogen and acetate. Hence specific digestion conditions that enrich for these populations may provide a route to the optimization of co-digestion systems and control the variability in reactor performance.

关键词： Anaerobic Co-Digestion Bacteroidetes Cellulose Degrading Bacterial Community

Combinational adenovirus-mediated gene therapy and dendritic cell vaccine in combating well-established tumors

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Cell Research 2006年第3期16卷 241-259页

作者： terence Moyana Jim Xiang Department of Pathology and laboratory Medicine University of OttawaOttawaOntario K1H 8L6Canada Research Unit Health Research DivisionSaskatchewan Cancer AgencyDepartments of Oncology.Microbiology and Immunology.University of Saskatchewan20 Campus DriveSaskatoonSaskatchewan STN 4H4Canada

Recent developments in tumor immunology and biotechnology have made cancer gene therapy and *** current efforts for cancer gene therapy mainly focus on using immunogenes,chemogenes and tumor sup-pressor *** to all these therapies is the development of efficient vectors for gene *** far,adenovirus(AdV)-mediated gene therapy is one of the most promising approaches,as has confirmed by studies relating to animaltumor models and clinical *** cells (DCs) are highly efficient,specialized antigen-presenting cells,and DC-based tumor vaccines are regarded as having much potential in cancer *** with DCs pulsedwith tumor peptides,Iysates,or RNA,or loaded with apoptotic/necrotic tumor cells,or engineered to express certaincytokines or chemokines could induce significant antitumor cytotoxic T lymphocyte (CTl) responses and *** both AdV-mediated gene therapy and DC vaccine can both stimulate antitumor immune responses,their therapeutic efficiency has been limited to generation of prophylactic antitumor immunity against re-challenge withthe parental tumor cells or to growth inhibition of small ***,this approach has been unsuccessful in com-bating welt-established tumors in animal ***,a major strategic goal of current cancer immunotherapy hasbecome the development of novel therapeutic strategies that can combat welt-established tumors,thus resembling realclinical practice since a good proportion of cancer patients generally present with significant *** this paper,wereview the recent progress in AdV-mediated cancer gene therapy and DC-based cancer vaccines,and discuss combinedimmunotherapy including gene therapy and DC *** underscore the fact that combined therapy may have someadvantages in combating well-established tumors vis-a-vis either modality administered as a monotherapy.

关键词： gene therapy adenovirus dendritic cells vaccine cytotoxic T lymphocytes antitumor immunity

Defect of CD8+ Memory T Cells Developed in Absence of Il-12 Priming for Secondary Expansion

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Cellular & Molecular Immunology 2008年第2期5卷 147-152页

作者： terence Moyana Jim Xiang Department of Pathology University of OttawaOntario K1H 8L6Canada Department of Oncology University of SaskatchewanSaskatoonSaskatchewan S7N 4H4Canada

Il-12 priming plays an important role in stimulation of CD8+ effector T cells and development of CD8+ memory T (Tm) cells. However,the functional alteration of CD8+ Tm cells developed in the absence of Il-12 priming is elusive. In this study,we investigated the capacity of secondary expansion of CD8+ Tm cells developed from transgenic OT I CD8+ T cells. The latter cells were in vitro and in vivo stimulated by ovalbumin (OVA)-pulsed dendritic cells DCOVA and (Il-12-/-)DCOVA derived from wild-type C57Bl/6 and Il-12 gene knockout mice,respectively. We demonstrated that Il-12 priming is important not only in CD8+ T cell clonal expansion,but also in generation of CD8+ Tm cells with the capacity of secondary expansion upon antigen re-encounter. However,Il-12 signaling is not involved in CD8+ Tm cell survival and recall responses. Therefore,this study provides useful information for vaccine design and development.

关键词： Il-12 effector CD8+ T cell memory T cell recall response

The Private Benefits of Corporate Control:Evidence from China

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Economic and Political Studies 2014年第1期2卷 44-64页

作者： XIAOJIN lIU terence T.l.CHONG the Department of Economics The Chinese University of Hong Kong

By analyzing block share transfers in China during 1999-2006,this study provides estimates of the private benefits of corporate control in *** find that those controlling blocks are usually priced at a significant positive premium compared to those comparable non-controlling *** benefits of corporate control vary with shareholders structure,firm characteristics and institutional *** particular,targets with a high intangible asset ratio have less benefits of control,cross-listing in B or H shares curbs the extract of private benefits and companies acquired by private firms are associated with higher private ***,it is found that benefits of control are negatively associated with market economy and factor market development *** argue that firms with higher private benefits experience more tunneling activities by their controlling shareholders.

关键词： private benefits of control block share transfers Chinese stock market

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Small cell lung carcinoma metastatic to the stomach: Commonly overlooked, limited treatment options

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World Journal of Gastroenterology 2024年第48期30卷 5198-5204页

作者： terence N Moyana Diagnostic and Molecular Pathology The Ottawa Hospital and University of OttawaOttawa K1H 8L6OntarioCanada

Small cell lung carcinoma metastatic to the stomach,whether synchronous or metachronous,is a rare phenomenon accounting for<0.5%of lung *** it can be overlooked by clinicians resulting in delayed *** manuscript commen... 详细信息

Small cell lung carcinoma metastatic to the stomach,whether synchronous or metachronous,is a rare phenomenon accounting forlung *** it can be overlooked by clinicians resulting in delayed *** manuscript comments on Yang et al’s article which reported 3 such *** main diagnostic features are based on routine morphology comprised of small cells with hyperchromatic nuclei,scant cytoplasm,brisk mitoses and *** can be supplemented by immunohistochemistry demonstrating positivity for cytokeratin,thyroid transcription factor-1 and neuroendocrine markers as well as a high Ki-67 labelling *** modalities such as positron emission tomography/contrast computed tomography help to confirm lung origin and rule out the possibility of extra-pulmonary small cell *** predominant mechanism of spread is most likely *** is generally poor since this represents stage 4 disease but survival can be improved by chemo/ra-diotherapy and palliative surgery in select *** outcomes have not changed much in the last several decades,the recent Food and Drug Adminis-tration approval of immune checkpoint inhibitors was a significant milestone as was the delineation of small cell lung carcinoma molecular *** biopsies are increasingly being used for biomarker studies in clinical trials to assess treatment response and prognosis.

关键词： Gastric metastasis Small cell lung carcinoma Histopathology Immuno-histochemistry Positron emission tomography/contrast computed tomography Chemo-radiotherapy Immunotherapy Surgery Biomarkers Clinical trials

Metabolic dysfunction-associated steatotic liver disease:The question of long-term high-normal alanine aminotransferase as a screening test

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World Journal of Gastroenterology 2024年第42期30卷 4576-4582页

作者： terence N Moyana Department of Pathology and laboratory Medicine University of Ottawa and The Ottawa HospitalOttawa K1H 8L6OntarioCanada

The growing prevalence of metabolic dysfunction-associated steatotic liver disease(MASlD)is being driven by the obesity *** quest for solutions continues particularly with regard to early *** editorial comments on the utility of long-term high-normal alanine aminotransferase(AlT)in screening for *** et al found that new onset MASlD can be detected by repetitively high normal *** in this concept is the question of what should be the accepted upper limit of normal(UlN)for *** was previously set at 40 IU/l based on studies that included people with subclinical liver disease but the new consensus is 30/19 U/l in healthy males/***,when Chen et al defines the UlN as 40 U/l,others may view it as excessively *** is important to recognize the variables affecting UlN ***,diurnal variations,exercise and *** variables matter when the distinctions are subtle *** vs *** this regard,the utility of long-term high normal AlT as a disease marker could be enhanced by combining it with other biomarkers,imaging and MASlD genetics to create machine learning *** in all,Chen et al’s work on long-term high normal AlT as a marker of new-onset MASlD deserves merit.

关键词： Metabolic dysfunction-associated steatotic liver disease Alanine aminotransferase Pathophysiology Screening Upper limit of normal Biomarkers Predictive models