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Intracellular calcium ions facilitate dengue virus entry into endothelial cells and compromise endothelial barrier integrity

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Asian Pacific Journal of Tropical Medicine 2021年第11期14卷 505-516页

作者： Meng-Hooi Shu Pooi-fong wong Sing-Sin Sam Shih-Keng Loong Boon-Teong Teoh Sazaly AbuBakar Tropical Infectious Diseases Research and Education Centre(TIDREC) Universiti Malaya50603 Kuala LumpurMalaysia Department of Pharmacology Faculty of MedicineUniversiti Malaya50603 Kuala LumpurMalaysia World Health Organization Collaborating Centre for Arbovirus Reference and Research(Dengue and Severe Dengue)MAA-12 Universiti Malaya50603 Kuala LumpurMalaysia

Objective:To investigate the involvement of Ca^(2+)in dengue virus(DENV)-infected human umbilical vein endothelial cells(HUVECs)and the disruption of endothelial ***:HUVECs were infected with DENV-2 in the presence of intracellular Ca^(2+)or endoplasmic reticulum Ca^(2+)*** infectivity was measured by focus-forming assay and quantitative *** Ca^(2+)was measured using Fluo-4-AM ***-cadherin and focal adhesion kinase(FAK)expressions were investigated by immunofluorescence and immunoblotting assays,***:DENV infection increased intracellular cytosolic Ca^(2+)levels and caused disassembly of the adherens junction protein,VEcadherin as evidenced by decreased VE-cadherin expression at the periphery of DENV-2 infected *** of intracellular Ca^(2+)stores,particularly those of the endoplasmic reticulum Ca^(2+),significantly decreased DENV yield in *** virus yield following the depletion of intracellular Ca^(2+)was caused by the inhibition of viral entry into HUVECs and not the inhibition of viral binding or ***-2 infection also resulted in Ca^(2+)-dependent activation of ***:Intracellular Ca^(2+)is required for the early phases of DENV infection in endothelial *** cytosolic Ca^(2+)levels in endothelial cells during DENV infection activated FAK,disrupted adherens junctions and compromised barrier ***,Ca^(2+)plays an important role in DENV infection in endothelial cells.

关键词： Endothelial cells Calcium signalling Dengue virus Endothelium permeability Intracellular calcium

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MicroRNA-142-3p and microRNA-142-5p are downregulated in hepatocellular carcinoma and exhibit synergistic effects on cell motility

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Frontiers of Medicine 2015年第3期9卷 331-343页

作者： Felice Ho-Ching Tsang Sandy Leung-Kuen Au Lai Wei Dorothy Ngo-Yin Fan Joyce Man-fong Lee Carmen Chak-Lui wong Irene Oi-Lin Ng Chun-Ming wong State Key Laboratory for Liver Research and Department of Pathology Li Ka Shing Faculty of Medicine The University of Hong Kong HongKong SAR China

MicroRNAs （miRNAs）, an important class of small non-coding RNAs, regulate gene expression at the post-transcriptional level, miRNAs are involved in a wide range of biological processes and implicated in different diseases, including cancers. In this study, miRNA profiling and qRT-PCR validation revealed that miR-142-3p and miR-142-5p were significantly downregulated in hepatocellular carcinoma （HCC） and their expression levels decreased as the disease progressed. The ectopic expression of miR-142 significantly reduced HCC cell migration and invasion. Overexpression of either miR-142-3p or miR-142-5p suppressed HCC cell migration, and overexpression of both synergistically inhibited cell migration, which indicated that miR-142-3p and miR-142-5p may cooperatively regulate cell movement, miR-142-3p and miR-142-5p, which are mature miRNAs derived from the 3＇- and 5＇-strands of the precursor miR-142, target distinct pools of genes because of their different seed sequences. Pathway enrichment analysis showed a strong association of the putative gene targets of miR-142-3p and miR-142-5p with several cell motility-associated pathways, including those regulating actin cytoskeleton, adherens junctions, and focal adhesion. Importantly, a number of the putative gene targets were also significantly upregulated in human HCC cells. Moreover, overexpression of miR-142 significantly abrogated stress fiber formation in HCC cells and led to cell shrinkage. This study shows that mature miR-142 pairs collaboratively regulate different components of distinct signaling cascades and therefore affects the motility of HCC cells.

关键词： hepatocellular carcinoma microRNA metastasis cytoskeletal reorganization

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Status monitoring and health assessment of Luhuitou fringing reef of Sanya, Hainan, China

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Chinese Science Bulletin 2006年第B12期51卷 81-88页

作者： ZHANG Qiaomin SHI Qi CHEN Gang fong T C W wong D C C HUANG Hui WANG Hankui ZHAO Meixia South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 China Kay Laboratory of Marginal Sea Geology Chinese Aeademy of Sciences Guangzhou 510301 China Department of Marine and Fishery of Hainan Province Haikou 570203 China Environmental Resources Management Hong Kong China Hong Kong Cetacean Research Project Hong Kong China Tropical Marine Biological Research Station in Halnan Chinese Academy of Sciences Sanya 572000 China

The monitoring survey and health assessment for benthic community were carried out in Luhuitou fringing reef of Sanya, Hainan, China in 2002. Monitoring survey included manta tow, line intercept transect, video transect and digital photograph. The mean live coral cover was 23.40%. Comparison of used monitoring methods showed that video transect can replace line intercept transect, and 50 m transect is better than 20 m transect in uneven coral region. Survey data and comparison with previous research work showed that Luhuitou fringing reef has been damaged severely and has a general declining trend with live coral cover from about 85% in the 1960s down to 20% in 2004, but it still has quite high biodiversity and therefore has special protection value. Main factors of Luhuitou fringing reef declining were human activities including reef rock digging and curios collecting, destructive fishing, sediment and pollutant from coastal land, and recent marine aquaculture. Only strengthening integrated management can reach improvement and restoration of Luhuitou declining coral reef ecosystem.

关键词：海南岛三亚鹿回头珊瑚礁生物多样性环境监测健康评估

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A CHALLENGE OF THE EXPERIMENT ON TYPHOON INTENSITY CHANGE IN COASTAL AREA

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Tropical Cyclone Research and Review 2017年第3期6卷 94-97页

作者： XIAOTU LEI WAIKIN wong CLARENCE fong Shanghai Typhoon Institute of China Meteorological Administration Hong Kong Observatory ESCAP/WMO Typhoon Committee Secretariat

The Experiment on Typhoon Intensity Change in Coastal Area(EXOTICCA) was proposed by the China Meteorological Administration(CMA) and Hong Kong Observatory(HKO) and endorsed by the ESCAP/WMO Typhoon Committee(TC). The major goals and objectives of the EXOTICCA are: 1) to conduct the field campaigns on the intensity and structural characteristics of the target offshore and landfall tropical cyclones by employing integrated and novel observation techniques, and 2) to conduct demonstration research on the utilization of the synergized field observation data with the aim of deepening the understanding of the mechanism of structure and intensity changes, to improve the relevant capability of operational analysis, numerical weather prediction(NWP) models forecast, reliable storm surge and flooding and associated risk assessment. The Organizational structure and implementation schedule etc. are also introduced in this paper.

关键词： tropical cyclone intensity experiment field campaign numerical weather prediction(NWP)

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3D printing for ultra-precision machining:current status,opportunities,and future perspectives

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Frontiers of Mechanical Engineering 2024年第4期19卷 1-28页

作者： Tao HE Wai Sze YIP Edward Hengzhou YAN Jiuxing TANG Muhammad REHAN Long teng Chi Ho wong Linhe SUN Baolong ZHANG Feng GUO Shaohe ZHANG Suet TO State Key Laboratory of Ultra-precision Machining Technology Department of Industrial and Systems EngineeringThe Hong Kong Polytechnic UniversityHong KongChina Department of Industrial and Systems Engineering The Hong Kong Polytechnic UniversityHong KongChina Department of Physics The Hong Kong University of Science and TechnologyHong KongChina School of Geosciences and Info-Physics Central South UniversityChangsha 410083China

Additive manufacturing,particularly 3D printing,has revolutionized the manufacturing industry by allowing the production of complex and intricate parts at a lower cost and with greater ***,3D-printed parts frequently require post-processing or integration with other machining technologies to achieve the desired surface finish,accuracy,and mechanical ***-precision machining(UPM)is a potential machining technology that addresses these challenges by enabling high surface quality,accuracy,and repeatability in 3D-printed *** study provides an overview of the current state of UPM for 3D printing,including the current UPM and 3D printing stages,and the application of UPM to 3D *** the presentation of current stage perspectives,this study presents a detailed discussion of the benefits of combining UPM with 3D printing and the opportunities for leveraging UPM on 3D printing or supporting each *** particular,future opportunities focus on cutting tools manufactured via 3D printing for UPM,UPM of 3D-printed components for real-world applications,and post-machining of 3D-printed ***,future prospects for integrating the two advanced manufacturing technologies into potential industries are *** study concludes that UPM is a promising technology for 3D-printed components,exhibiting the potential to improve the functionality and performance of 3D-printed products in various *** also discusses how UPM and 3D printing can complement each other.

关键词： ultra-precision machining 3D printing additive manufacturing future perspectives start-of-the-art-review

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Trabecular bone deterioration at the greater trochanter ol mice with unilateral obstructive nephropathy

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Asian Journal of Andrology 2013年第4期15卷 564-566,I0011,I0012页

作者： Sa-Sa Gu Yan Zhang Xi Chen teng-Yue Diao Yoseph Gebru Man-Sau wong Center tor Systems Biomedical Sciences University ot Shanghai tor Science and I echnology Shanghai 200093 China School ot Medical Instrument and Food Engineering University of Shanghai for Science and Technology Shanghai 200093 China and Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic University Hung Horn Kowloon Hong Kong SAR China

Our previous study showed the early molecular responses of bone in response to obstructive nephropathy in a unilateral ureteral obstruction （UUO） mouse model. Here, we addressed the changes in trabecular bone properties at greater trochanter, the proximal and the distal metaphysis of femur in UUO mice. The male mice were subjected to UUO （n= 10） or sham operation （n= 10）. All mice were killed on day 7 after the surgical operation. The micro-computed tomography （micro-CT） analysis for different femoral trabecular bone sites demonstrated pathological alterations of trabecular bone mass and micro-networks at greater trochanter as shown by decreases in bone mineral density/bone volume （P〈O.05） and trabecular number （P〈O.05） and increases in trabecular separation （P〈O.01） and bone surface/bone volume （P〈O.05） in UUO mice. The present study demonstrates that UUO-induced unilateral obstructivenephropathy has markedly detrimental effects on the trabecular trochanter of the femur.

关键词： bone femur micro-computed tomography (micro-CT) trochanter unilateral ureteral obstruction (UUO)

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Defect-rich potassium amide: A new solid-state potassium ion electrolyte

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Journal of Energy Chemistry 2022年第6期31卷 555-560,I0015页

作者： Jiang Wang Gangtie Lei teng He Hujun Cao Ping Chen Dalian Institute of Chemical Physics Chinese Academy of SciencesDalian 116023LiaoningChina Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education School of ChemistryXiangtan UniversityXiangtan 411105HunanChina

One of the major obstacles to the application of potassium-ion batteries in large-scale energy storage is the lack of safe and effective ***_(2),a new potassium-ion solid electrolyte has been developed in this *** ionic conductivity reaches 4.84×10^(-5)S cm^(-1)at 150°C and can reach3.56×10^(-4)S cm^(-1)after mechanochemical *** result from electron paramagnetic resonance(EPR) measurement shows that the increment of ionic conductivity is dependent on the concentration of nitrogen defects in the KNH_(2) *** the best of our knowledge,this is the first report that adopts inorganic amide as an electrolyte for potassium-ion battery and initiates the search for a new amidebased solid electrolyte for an all-solid-state potassium-ion battery.

关键词： Solid-state conductor Potassium ionic conductivity Nitrogen defect Potassium amide

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Influence of matrix-metalloproteinase inhibitor on the interaction between cancer cells and matrigel

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Chinese Physics B 2020年第6期29卷 75-78页

作者： teng Ye Fangfu Ye Feng Qiu First Clinical Medical College Nanchang UniversityNanchang 330006China Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China Wenzhou Institute University of Chinese Academy of SciencesWenzhou 325001China Department of Oncology First Affiliated Hospital of Nanchang UniversityNanchang 330006China

Various behaviors of cancer cells are strongly influenced by their interaction with extracellular matrices(ECM).We investigate how this interaction may be influenced if the cancer cells’ability of secreting matrix metalloproteinases(MMPs)to degrade ECM is inhibited by adding the MMP *** useMDA-MB-231-GFP cells as model cells and use matrigel to mimic *** is found that the added MMP inhibitor significantly reduces the migration speed of cancer cells covered by matrigel but has little influence on the migration persistence and shape factor of the cells and that with the MMP inhibitor added the presence of matrigel on the top has no influence on the migration speed of the cells but increases the cells’shape factor and migration persistence.

关键词： cancer cells matrigel matrix metalloproteinase(MMP)inhibitor migration

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Synthesis and mesomorphic properties of new Schiff base esters with different alkyl chains

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Chinese Chemical Letters 2009年第7期20卷 767-770页

作者： Sie Tiong Ha Lay Khoon Ong Siew teng Ong Guan Yeow Yeap Joanna Pik Wan wong Teck Ming Koh Hong Cheu Lin Faculty ofEngineering & Science Universiti Tunku Abdul RahmanJln Genting KlangSetapak 53300 Kuala LumpurMalaysia Faculty of Engineering & Science Universiti Tunku Abdul RahmanJln Genting KlangSetapak 53300 Kuala LumpurMalaysia Liquid Crystal Research Laboratory School of Chemical SciencesUniversiti Sains Malaysia 11800 MindenPenangMalaysia Department of Chemistry University of YorkHeslingtonYork YO10 5DDUnited Kingdom.

A new series of Schiff base esters, 4-（dimethylamino）benzylidene-4＇-alkanoyloxyanilines containing even number of carbons at the end group of the molecules （Cn-1H2n-1COO, n = 6, 8, 10, 12, 14, 16, 18） were synthesized. The present compounds were monotropic liquid crystals. It was also found that the end groups of the molecules had effect on the mesomorphic properties. C 2009 Sie Tiong Ha. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

关键词： Liquid crystals Schiff base esters Monotropic compounds Mesomorphic properties

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Agentic Response to Institutional Constraints in Tourism and Hospitality Sectors

Agentic Response to Institutional Constraints in Tourism and...

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2019年第四届经济与企业管理国际学术会议

作者： Xiaoyu Wu Veronica Hoi in fong IpKin Anthony wong School of Business Macau University of Science and Technology School of Tourism Management Sun Yat-Sen University

Institutionalism has demonstrated the behaviours of legitimacy,prevailing social rules and practices in tourism and hospitality *** central idea is that organizations conform to the rules and belief systems in the institutional environment,in particular constraints or pressures because of *** this,little is known about the human agency in responding their institutional constraints and changing the embedded *** is important to understand how different individual agencies respond to the constraints that brought by the tourism policies,arrangements and administrations,and how these responses influence the change *** issues are explored from the agency perspective that considers the responses of the individual agencies which shape different degree of changes when institutional constraints are *** perspective is applied through a quality study to illustrate the government,industry and organizations involvement in tourism and hospitality reforms in Macau between 2002 and 2017.

关键词： Institutional constraints agentic responses tourism and hospitality reform

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