Intracellular calcium ions facilitate dengue virus entry into endothelial cells and compromise endothelial barrier integrity
作者机构：Tropical Infectious Diseases Research and Education Centre(TIDREC)Universiti Malaya50603 Kuala LumpurMalaysia Department of PharmacologyFaculty of MedicineUniversiti Malaya50603 Kuala LumpurMalaysia World Health Organization Collaborating Centre for Arbovirus Reference and Research(Dengue and Severe Dengue)MAA-12Universiti Malaya50603 Kuala LumpurMalaysia
出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)
年 卷 期：2021年第14卷第11期
页 面：505-516页
核心收录：
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学]
基 金：funding from the Ministry of Higher Education Malaysia via the Higher Institution Centre of Excellence(HICo E)program(MO002-2019) Development of Research Institute for Excellent Enterprises(ATC+)Project,Republic of Korea(IF001-2021)
主 题：Endothelial cells Calcium signalling Dengue virus Endothelium permeability Intracellular calcium
摘 要：Objective:To investigate the involvement of Ca^(2+)in dengue virus(DENV)-infected human umbilical vein endothelial cells(HUVECs)and the disruption of endothelial ***:HUVECs were infected with DENV-2 in the presence of intracellular Ca^(2+)or endoplasmic reticulum Ca^(2+)*** infectivity was measured by focus-forming assay and quantitative *** Ca^(2+)was measured using Fluo-4-AM ***-cadherin and focal adhesion kinase(FAK)expressions were investigated by immunofluorescence and immunoblotting assays,***:DENV infection increased intracellular cytosolic Ca^(2+)levels and caused disassembly of the adherens junction protein,VEcadherin as evidenced by decreased VE-cadherin expression at the periphery of DENV-2 infected *** of intracellular Ca^(2+)stores,particularly those of the endoplasmic reticulum Ca^(2+),significantly decreased DENV yield in *** virus yield following the depletion of intracellular Ca^(2+)was caused by the inhibition of viral entry into HUVECs and not the inhibition of viral binding or ***-2 infection also resulted in Ca^(2+)-dependent activation of ***:Intracellular Ca^(2+)is required for the early phases of DENV infection in endothelial *** cytosolic Ca^(2+)levels in endothelial cells during DENV infection activated FAK,disrupted adherens junctions and compromised barrier ***,Ca^(2+)plays an important role in DENV infection in endothelial cells.
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