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Efficacy and safety of generic daclatasvir +sofosbuvir ± ribavirin in treatment of genotype 3infected hepatitis C patients - a real life experience from Pakistan

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Hepatoma Research 2018年第11期4卷 32-38页

作者： Muhammad Umar tayyab saeed akhter Junaid Sadiq Samar Saleem Shoaib Khokhar Centre for Liver and Digestive Diseases Holy Family HospitalRawalpindi Medical College and Allied HospitalsRawalpindi46300Pakistan Tehsil Headquarter Hospital Murree47150Pakistan

Aim: Genotype 3 is the most prevalent genotype in Pakistan. Despite a revolution in the treatment of Hepatitis C, genotype 3 is still thought to be difficult to treat genotype. The price of patent direct acting antivirals was thought to a great limiting factor especially for low income countries. In Pakistan low cost generics of daclatasvir and sofosbuvir are easily available for treatment. The aim of our study is to provide real life local data to determine their efficacy and safety. Methods: This open-label, non-randomized, uncontrolled study was carried out at Center for Liver and Digestive Diseases, Holyfamily Hospital, Rawalpindi. We enrolled patients from March 2016 through March 2018 who were 18 years or older having chronic hepatitis C infection with detectable polymerase chain reaction (PCR), regardless of whether they were treatment na?ve or have experienced Interferon in the past. The patients were offered generic sofosbuvir 400 mg and daclatasvir 60 mg once daily with or without ribavirin for a period of 12 to 24 weeks. Follow-up PCRs were performed at 4th week of treatment, end of treatment and 12 weeks post treatment. All those patients were included in the study that had at least one follow-up PCR during or after the course of treatment. Results: A total of 102 patients were enrolled in the study with a mean age of 48.11 ± 12.70 including 63% males and 37% females. All patients were genotype 3. On 4th week follow up, 31/36 (86.11%) patients had quantitative PCR negative. Out of 102 patients 78 patients had follow up PCR at the completion of therapy with an end of treatment response of about 96.1%. Thirty patients had a follow up at 12 weeks post treatment with a SVR12 of 83.33% (25/30) amongst which treatment Na?ve had a response rate of 84% (21/25), treatment experience 80%(4/5), non-cirrhotics 85.71% (12/14), cirrhotics 81.25% (13/16) and decompensated chronic liver disease patients have a SVR12 of about 83.33% (10/12) respectively. The combina

关键词： Daclatasvir sofosbuvir genotype 3 hepatitis C

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Risk factor profiles for gastric cancer prediction with respect to Helicobacter pylori:A study of a tertiary care hospital in Pakistan

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Artificial Intelligence in Gastroenterology 2023年第1期4卷 10-27页

作者： Shahid Aziz Simone König Muhammad Umer tayyab saeed akhter Shafqat Iqbal Maryum Ibrar Tofeeq Ur-Rehman Tanvir Ahmad Alfizah Hanafiah Rabaab Zahra Faisal Rasheed Patients Diagnostic Lab Isotope Application DivisionPakistan Institute of Nuclear Science and TechnologyIslamabad 44000Pakistan Department of Microbiology Quaid-i-Azam UniversityIslamabad 45320Pakistan Interdisciplinary Center for Clinical Research Core Unit ProteomicsUniversity of MünsterMünster 48149Germany Management Information System Division Pakistan Institute of Nuclear Science and TechnologyIslamabad 44000Pakistan Centre for Liver and Digestive Diseases Holy Family HospitalRawalpindi 46300Pakistan Pakistan Scientific and Technological Information Centre Quaid-i-Azam UniversityIslamabad 45320Pakistan Department of Pharmacy Quaid-i-Azam UniversityIslamabad 45320Pakistan Faculty of Medicine Department of Medical Microbiology and ImmunologyUniversiti Kebangsan MalaysiaCherasKuala Lumpur 56000Malaysia

BACKGROUND Gastric cancer(GC)is the fourth leading cause of cancer-related deaths *** relies on histopathology and the number of endoscopies is *** pylori(***)infection is a major risk *** To develop an in-silico GC prediction model to reduce the number of diagnostic surgical *** meta-data of patients with gastroduodenal symptoms,risk factors associated with GC,and *** infection status from Holy Family Hospital Rawalpindi,Pakistan,were used with machine *** A cohort of 341 patients was divided into three groups[normal gastric mucosa(NGM),gastroduodenal diseases(GDD),and GC].Information associated with socioeconomic and demographic conditions and GC risk factors was collected using a *** infection status was determined based on urea breath *** association of these factors and histopathological grades was assessed statistically.K-Nearest Neighbors and Random Forest(RF)machine learning models were *** This study reported an overall frequency of 64.2%(219/341)of *** infection among enrolled *** was higher in GC(74.2%,23/31)as compared to NGM and GDD and higher in males(54.3%,119/219)as compared to *** abdominal pain(72.4%,247/341)was observed than other clinical symptoms including vomiting,bloating,acid reflux and *** majority of the GC patients experienced symptoms of vomiting(91%,20/22)with abdominal pain(100%,22/22).The multinomial logistic regression model was statistically significant and correctly classified 80%of the GDD/GC ***,income level,vomiting,bloating and medication had significant association with GDD and GC.A dynamic RF GC-predictive model was developed,which achieved>80%test *** GC risk factors were incorporated into a computer model to predict the likelihood of developing GC with high sensitivity and *** model is dynamic and will be further improved and validated by including n

关键词： Gastric cancer Gastritis Machine learning Prediction model Helicobacter pylori

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A novel method for locating the source of sustained oscillation in power system using synchrophasors data

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Protection and Control of Modern Power Systems 2020年第1期5卷 340-351页

作者： Mohd Zuhaib Mohd Rihan Mohd tayyab saeed Electrical Engineering Department Zakir Husain College of Engineering and TechnologyAligarh Muslim University(AMU)AligarhIndia Al-Mostajed Technologies Pvt Ltd DubaiUAE

Large interconnected power systems are usually subjected to natural oscillation(NO)and forced oscillation(FO).NO occurs due to system transient response and is characterized by several oscillation modes,while FO occurs due to external perturbations driving generation *** to NO,FO is considered a more severe threat to the safe and reliable operation of power ***,it is important to locate the source of FO so corrective actions can be taken to ensure stable power system *** this paper,a novel approach based on two-step signal processing is proposed to characterize FO in terms of its frequency components,duration,nature,and the location of the *** recorded by the Phasor Measurement Units(PMUs)in a Wide Area Monitoring System(WAMS)is utilized for *** PMU data usually contains white noise and appears as multi-frequency oscillatory signal,the first step is to de-noise the raw PMU data by decomposing it into a series of intrinsic mode functions(IMF)using Improved Complete Ensemble Empirical Mode Decomposition with Adaptive Noise(ICEEMDAN)*** most appropriate IMF containing the vital information is selected using the correlation *** second step involves various signal processing and statistical analysis tools such as segmented Power Spectrum Density(PSD),excess kurtosis,cross PSD *** achieve the desired *** analysis performed on the simulated two-area four-machine system,reduced WECC-179 bus 29 machine system,and the real-time power system PMU data set from ISO New England,demonstrates the accuracy of the proposed *** proposed approach is independent of complex network topologies and their characteristics,and is also robust against measurement noise usually contained in PMU data.

关键词： Forced oscillation(FO) Natural oscillation(NO) Phasor measurement unit(PMU) Wide area monitoring system(WAMS) Empirical mode decomposition(EMD)etc

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Control Charts for the Shape Parameter of Power Function Distribution under Different Classical Estimators

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Computer Modeling in Engineering & Sciences 2021年第6期127卷 1201-1223页

作者： Azam Zaka Ahmad saeed akhter Riffat Jabeen Aamir Sanaullah College of Statistical&Actuarial Sciences University of the PunjabLahore54000Pakistan COMSATS University Islamabad Lahore CampusLahore54000Pakistan

In practice,the control charts for monitoring of process mean are based on the normality *** the performance of the control charts is seriously affected if the process of quality characteristics departs from *** such situations,we have modified the already existing control charts such as Shewhart control chart,exponentially weighted moving average(EWMA)control chart and hybrid exponentially weighted moving average(HEWMA)control chart by assuming that the distribution of underlying process follows Power function distribution(PFD).By considering the situation that the parameters of PFD are unknown,we estimate them by using three classical estimation methods,i.e.,percentile estimator(P.E),maximum likelihood estimator(MLE)and modified maximum likelihood estimator(MMLE).We construct Shewhart,EWMA and HEWMA control charts based on P.E,MLE and *** have compared all these control charts using Monte Carlo simulation studies and concluded that HEWMA control chart under MLE is more sensitive to detect an early shift in the shape parameter when the distribution of the underlying process follows power function distribution.

关键词： Average run length control chart percentile estimator power function distribution

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Synthesis, Crystal Structure, Cytotoxic, Antileishmanial and Docking Evaluation of 3-(4-Chloro-3-nitrophenyl)-1-phenylprop-2-en-1-one

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Chinese Journal of Structural Chemistry 2018年第8期37卷 1250-1258页

作者： HAMID Aziz AAMER saeed FARUKH Jabeen ULRICH Florke QURAT-Ul-Ain NADHMAN akhter Department of Chemistry Quaid-I-Azam University Islamabad 45320 Pakistan Cardiovascular and Metabolic Research Unit Laurentian University 935 Ramsey Lake Road Sudbury ON Canada P3E 2C6 Department Chemie Fakultatfur Naturwissenschafien Universitat Paderborn Warburgerstrαβe 100 33098 Paderborn Germany Center for Interdisciplinary Research in Basic Sciences IIU Islamabad Pakistan

Chalcone derivatives are of immense importance owing to their superior pharmacological profile. Minor changes in their structures create high degree of diversity that results in a broader spectrum of biological activities. The present research paper presents the synthesis of 3-（4-chloro-3-nitrophenyl）-1-phenylprop-2-en-1-one chalcone（I）, single crystal XRD analysis and the evaluation of its possible biological activities like cytotoxic（LD（50） = 5.40 ug/m L）and antileishmanial（LD（50） = 0.81 ug/m L） activities. The results indicate the tested chalcone（I）shows superior inhibition values against leishmanial promastigotes. Further, the possible interaction sites of chalcone（I） with Try R enzyme involved in the redox metabolism of the leishmanial parasite were determined using docking simulation technique. Docking computations demonstrate that the compound establishes prominent binding interactions with the key residues of Try R and possesses the potential to effectively inhibit the catalytic activities of enzyme. Thus the results suggest that the chalcone（I） can serve as a potential scaffold for the treatment of leishmaniasis and deserve further development.

关键词： chalcone cytotoxicity antfleishmanial Claisen-Schmidt condensation moleculardocking

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Intercomparison of Environmental Gamma doses Measured with A NaI(Tl) Survey Meter and Thermoluminescent Dosimeters(TLDs) in the Poonch Division of Azad Kashmir, Pakistan

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Biomedical and Environmental Sciences 2014年第12期27卷 969-972页

作者： Muhammad Rafique Kimberlee J Kearfott Khalil Ahmad Jabeen akhter Abdul Razzaq Khan Raja Azhar saeed saeed Ur Rahman Matiullah Muhammad Usman Rajput University of Michigan Department of Nuclear Engineering and Radiological Sciences Department of Physics University of Azad Jammu and Kashmir Muzaffarbad Radiation Dosimetry Laboratory Health Physics Division PINSTECH Department of Medical Physics Nuclear Medicine Oncology and Radiotherapy Institute Directorate of Systems and Services PINSTECH Physics Division Pakistan Institute of Nuclear Science and Technology (PINSTECH)

This study presents the intercomparison of the outdoor environmental gamma dose rates measured using a Nal （TI） based survey meter along with thermoluminescent dosimeters （TLDs） and estimation of excess lifetime cancer risk （ELCR）, for the inhabitants of Poonch division of the Azad Kashmir, Pakistan. CaF2： Dy （TLD-200） card dosimeters were installed at height of 1 m from ground at fifteen different locations covering the entire Poonch division comprising of three districts.

关键词： in the Poonch Division of Azad Kashmir Survey Meter and Thermoluminescent Dosimeters Tl TLDs NaI

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Prophet Muhammad (pbuh) Practices to Establish a Pluralistic Society: Study of Madīnah Charter in Contemporary Global Context

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Cultural and Religious Studies 2018年第9期6卷 548-560页

作者： Riaz Ahmad saeed Sumayyah Rafique Naseem akhter Zahid Lateef Abu Bakar Bhutta Hafiz Muhammad Naeem The National University of Modern Languages (NUML) Islamabad Pakistan Shaheed Benazir Bhutto Women University Peshawar Pakistan University of Engineering & Technology Lahore Pakistan National University of Modern Languages( NUML) Islamabad Pakistan Governmnet College University Lahore Pakistan

This study explores the practices of the Holy Prophet Muhammad ■ to establish a peaceful and diverse society with special reference to Methāq-e-Madīnah and its significance and application in contemporary socio-political context. Theoretically, the effort and dream to establish a peaceful and diverse society have been a matter of concern for the humanity from the ancient times. But it has become the most significant and burning issue of the contemporary global scenario. Ultimately, the human intellectual and physical development is based upon peace and peaceful coexistence. Therefore, a number of religious and socio-political scholars have been tried to establish a peaceful and diverse society in different phases of the human history. Practically, the Prophet Muhammad (■ )has made a unique and distinct contribution towards developing a peaceful and diverse society for 1,400 years ago, though he led or had been the part of multiethnic society of different faiths in the state of Madīnah. In order to establish a peaceful and pleasant relationship between Muslims and other communities of Madīnah, an agreement was signed which was titled Methāq-e-Madīnah. The Charter of Madīnah contained certain principles and regulations that are mandatory for a peaceful living in a diverse state or society. This charter is an excellent model for peace, prosperity, religious freedom, and human rights in the human history. According to this charter, all groups were free to exercise their religious beliefs and social and economic interests. According to this agreement, Madīnah was declared a federal capital of the state. This agreement had provided equal rights, religious autonomy, and socio-cultural freedom to all other groups of the Madīnah (Jews, Christians, and polytheists). Therefore, it is perceived that the Charter of Madīnah may become a preamble for peaceful coexistence in this multicultural and multipath world of the contemporary era. In this study, analytical research methodolo

关键词： prophet approach peaceful and diverse society Methāq-e-Madīnah modern era

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Economic and Social Impact Assessment of Chronic Diseases Among the Low-Income Population in Southern Punjab,Pakistan

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Proceedings of Anticancer Research 2024年第4期8卷 67-74页

作者： Mehran Khan Muhammad Arbab Nasir Muhammad Umar Asadullah Faiz Mirza Muhammad Abdul Bari Ayesha saeed Fiza Nazeer Khan Saliha Mehboob Malik Akashah Usama tayyab Bahawalpur College of Pharmacy BMDC Complex63100BahawalpurPunjabPakistan

Introduction:Chronic diseases are becoming more prevalent *** effects of chronic illnesses are disastrous not only for the diagnosed person but also for their entire *** study explores chronic diseases’social and economic impact on low-income *** study aims to determine the economic and social implications of various chronic diseases and the loss of income due to these conditions among low-income individuals in Southern Punjab,***:A sample of 424 patients was selected from different areas of Southern *** were collected using a questionnaire that included questions about economic status,self-reported health status,social status,management strategies,and health insurance,among other ***:The mean monthly income of the respondents was found to be 57,097.6 Pakistani rupee(PKR),and the mean monthly expenses for treatment were 8,256.1 *** loss of income was calculated at 15%.Additionally,62%of patients spent more than 10%of their monthly income on managing their *** 85%of the respondents reported that chronic diseases affected their social ***,80%of patients lacked health ***:Chronic diseases impose significant economic and social burdens on patients and their families in Southern *** reduce the burden of chronic diseases,the government should enhance healthcare services in this region and provide health insurance to low-income families.

关键词： Chronic diseases Low-income families Economic impact Punjab Pakistan

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Countrywide Survey for MERS-Coronavirus Antibodies in Dromedaries and Humans in Pakistan

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Virologica Sinica 2018年第5期33卷 410-417页

作者： Ali Zohaib Muhammad Saqib Muhammad Ammar Athar Jing Chen Awais-ur-Rahman Sial saeed Khan Zeeshan Taj Halima Sadia Usman Tahir Muhammad Haleem tayyab Muhammad Asif Qureshi Muhammad Khalid Mansoor Muhammad Ahsan Naeem Bing-Jie Hu Bilal Ahmed Khan Ikram Din Ujjan Bei Li Wei Zhang Yun Luo Yan Zhu Cecilia Waruhiu Iahtasham Khan Xing-Lou Yang Muhammad Sohail Sajid Victor Max Corman Bing Yan Zheng-Li Shi CAS Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Ctunese Academy of Sciences Beijing 100049China Faculty of Veterinary Medicine University of AgricultureFaisalabad 38040Pakistan Laboratory of Cancer Biomarkers and Liquid Biopsy College of PharmacyHenan UniversityHenan 475004China Department of Clinical Studies Faculty of Veterinary and Animal SciencesPMAS-Arid Agriculture UniversityRawalpindi 46000Pakistan Dow University of Health Science Pakistan Karachi 74200 Department of Microbiology Government College University FaisalabadFaisalabad 38000Pakistan Livestock and Dairy Development Department(L&DD) Lahore 54000Pakistan College of Veterinary and Animal Sciences The Islamia University of BahawalpurBahawalpur 63100Pakistan State Key Laboratory of Agricultural Mlcroblology Huazhong Agricultural UniversityWuhan 430070China Liaqat University of Medical and Health Science Pakistan Jamshoro 76090 Section of Epidemiology and Public Health Department of Chnical SciencesUniversity of Veterinary and Animal SciencesLahore subcampusJhang 35200Pakistan Institute of Virology Chadte-Universitatsmedizin BerlinCorporate member of Free UniversityHumboldt-University and Berlin Institute of HealthBerlin 10117Germany German Center for Infection Research(DZIF) Berlin 10117Germany

Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a zoonotic pathogen capable of causing severe respiratory disease in humans. Although dromedary camels are considered as a major reservoir host, the MERS-CoV infection dynamics in camels are not fully understood. Through surveillance in Pakistan, nasal (n = 776) and serum (n = 1050)samples were collected from camels between November 2015 and February 2018. Samples were collected from animal markets, free-roaming herds and abattoirs. An in-house ELISA was developed to detect IgG against MERS-CoV. A total of 794 camels were found seropositive for MERS-CoV. Prevalence increased with the age and the highest seroprevalence was recorded in camels aged [ 10 years (81.37%) followed by those aged 3.1–10 years (78.65%) and B 3 years (58.19%).Higher prevalence was observed in female (78.13%) as compared to male (70.70%). Of the camel nasal swabs, 22 were found to be positive by RT-qPCR though with high Ct values. Moreover, 2,409 human serum samples were also collected from four provinces of Pakistan during 2016–2017. Among the sampled population, 840 humans were camel *** we found a high rate of MERS-CoV antibody positive dromedaries (75.62%) in Pakistan, no neutralizing antibodies were detected in humans with and without contact to camels.

关键词： Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Camel -Human Pakistan

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National guidelines for the diagnosis and treatment of hilar cholangiocarcinoma

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World Journal of Gastroenterology 2024年第9期30卷 1018-1042页

作者： Faisal Saud Dar Zaigham Abbas Irfan Ahmed Muhammad Atique Usman Iqbal Aujla Muhammad Azeemuddin Zeba Aziz Abu Bakar Hafeez Bhatti Tariq Ali Bangash Amna Subhan Butt Osama Tariq Butt Abdul Wahab Dogar Javed Iqbal Farooqi Faisal Hanif Jahanzaib Haider Siraj Haider Syed Mujahid Hassan Adnan Abdul Jabbar Aman Nawaz Khan Muhammad Shoaib Khan Muhammad Yasir Khan Amer Latif Nasir Hassan Luck Ahmad Karim Malik Kamran Rashid Sohail Rashid Mohammad Salih Abdullah saeed Amjad Salamat Ghias-un-Nabi tayyab Aasim Yusuf Haseeb Haider Zia Ammara Naveed Department of Hepatopancreatic Biliary Surgery&Liver Transplant Pakistan Kidney and Liver Institute&Research CentreLahore 54000Pakistan Department of Hepatogastroenterology and Liver Transplantation Dr.Ziauddin University HospitalKarachi 75600SindhPakistan University of Aberdeen Aberdeen B243FXUnited Kingdom Department of Pathology Pakistan Kidney and Liver Institute&Research CentreLahore 54000Pakistan Department of Gastroenterology&Hepatology Pakistan Kidney and Liver Institute&Research CentreLahore 54000Pakistan Department of Radiology Aga Khan UniversityKarachi 74800Pakistan Department of Oncology Hameed Latif HospitalLahore 54000Pakistan Division of Hepatopancreatic Biliary Surgery&Liver Transplantation Shifa International HospitalIslamabad 44000Pakistan Department of Hepatopancreatic Biliary Surgery&Liver Transplant Shaikh Zayed Hospital and Post Graduate Medical InstituteLahore 54000Pakistan Department of Medicine Aga Khan University HospitalKarachi 74800Pakistan Department of Liver Transplant Pir Abdul Qadir Shah Jeelani Institute of Medical SciencesGambat 66020Pakistan Department of Medicine&Gastroenterology Lifecare Hospital and Research CentrePeshawar 25000Khyber PakhtunkhwaPakistan Department of Hepatopancreatobiliary&Liver Transplant Bahria International HospitalLahore 54000Pakistan Department of Surgery Hepatopancreatobiliary&Liver TransplantDow University of Health SciencesKarachi 74800Pakistan Department of Gastroenterology Hepatology&NutritionPir Abdul Qadir Shah Jeelani Institute of Medical SciencesGambat 66020Pakistan Department of Oncology Dr.Ziauddin HospitalKarachi 74800Pakistan Department of Radiology Rehman Medical InstitutePeshawar 25000Pakistan Army Liver Transplant Unit Pak Emirates Military HospitalRawalpindi 46000Pakistan Department of Gastroenterology Sindh Institute of Urology and TransplantationKarachi 75500Pakistan Rashid Nursing Home and Cancer Clinic Rashid Nursing Home and Cancer ClinicRawalpindi 46000Pakistan Department of Gastroentero

A consensus meeting of national experts from all major national hepatobiliary centres in the country was held on May 26,2023,at the Pakistan Kidney and Liver Institute&Research Centre(PKLI&RC)after initial consultations with the *** Pakistan Society for the Study of Liver Diseases(PSSLD)and PKLI&RC jointly organised this *** effort was based on a comprehensive literature review to establish national practice guidelines for hilar cholangiocarcinoma(hCCA).The consensus was that hCCA is a complex disease and requires a multidisciplinary team approach to best manage these *** coordinated effort can minimise delays and give patients a chance for curative treatment and effective *** diagnostic and staging workup includes high-quality computed tomography,magnetic resonance imaging,and magnetic resonance *** cytology or biopsy utilizing endoscopic retrograde cholangiopancreatography is a mainstay for ***,histopathologic confirmation is not always required before *** ultrasound with fine needle aspiration of regional lymph nodes and positron emission tomography scan are valuable adjuncts for *** only curative treatment is the surgical resection of the biliary tree based on the Bismuth-Corlette *** patients with unresectable hCCA can be considered for liver *** chemotherapy should be offered to patients with a high risk of *** use of preoperative biliary drainage and the need for portal vein embolisation should be based on local multidisciplinary *** with acute cholangitis can be drained with endoscopic or percutaneous biliary *** chemotherapy with cisplatin and gemcitabine has shown improved survival in patients with irresectable and recurrent hCCA.

关键词： Hilar cholangiocarcinoma Bismuth-Corlette classification Memorial Sloan Kettering Cancer Centre Staging Preoperative biliary drainage Portal vein embolisation Surgical resection Hepatectomy

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