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水解蛋白质化学改性(英文)

四川大学学报（工程科学版） 2002年第6期34卷 1-8页

作者：陈武勇 Brown E m taylor m m Cooke P H marmer W N 四川大学皮革化学与工程教育部重点实验室四川成都610065 美国农业部东部地区研究中心皮革室

从含铬废弃物提取的水解蛋白质与不同量的戊二醛 ,在不同的温度下反应。用聚酰胺凝胶电泳法 (SDS -PAGE)分析了改性蛋白质的分子量及分子量分布。用Canonmanning粘度法测定了改性产物的粘度。实验表明改性产物的分子量随戊二醛的用量增... 详细信息

从含铬废弃物提取的水解蛋白质与不同量的戊二醛 ,在不同的温度下反应。用聚酰胺凝胶电泳法 (SDS -PAGE)分析了改性蛋白质的分子量及分子量分布。用Canonmanning粘度法测定了改性产物的粘度。实验表明改性产物的分子量随戊二醛的用量增加而增加。最佳的戊二醛用量约为水解胶原质量的 12 % ,反应温度大于 6 0℃。同时 ,用改性产物做了皮革的填充实验 ,用萤光标记改性胶原 ,通过扫描电镜研究了填充皮革的纤维结构。研究结果表明 ,改性产物具有较好的填充性能 ,采用适当的应用工艺 ,改性产物可能用作皮革填充剂。

关键词：化学改性填充性能戊二醛水解蛋白质聚酰胺凝胶电泳法分子量粘度皮革填充剂

用微生物转谷氨酰胺酶对胶原水解产物酶法改性力学性能改良薄膜的制备

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北京皮革（中外皮革信息版）（中） 2005年第7期 92-97页

作者： taylor m.m 周南（编译）陈武勇（校）美国农业部东部研究中心四川大学生物质与皮革工程系2003级硕士研究生不详

本实验室先前的工作证实了，用微生物转谷氨酰胺酶对明胶酶法改性后，所制备的薄膜抗张强度较高，韧性较好，与单纯的明胶薄膜相比，其在水中的溶解度较低，并具有更好的亲水性。在现行的研究中，我们的目标是进一步改善酶法改性薄膜的... 详细信息

本实验室先前的工作证实了，用微生物转谷氨酰胺酶对明胶酶法改性后，所制备的薄膜抗张强度较高，韧性较好，与单纯的明胶薄膜相比，其在水中的溶解度较低，并具有更好的亲水性。在现行的研究中，我们的目标是进一步改善酶法改性薄膜的功能特性，并测定其是否还具有可生物降解性。取向拉伸薄膜是一种改进力学性能的方法。我们发现，不论是未改性或改性后的干明胶薄膜，还是经水中溶胀后在拉紧状态下干燥的薄膜，一旦被取向拉伸，其抗张强度、最大应变以及杨式模量均有明显改进。我们进一步证实了干燥温度对力学性能的影响：环境温度干燥下的产物与高温干燥下的产物相比，抗张强度明显较高而最大应变较低。我们还发现，在环境温度干燥下拉伸和未拉伸的薄膜抗张强度相似，由此可见干燥在薄膜制备中的重要性。最后，将聚乙烯醇与明胶共混，可制得抗张强度和最大应变改进的薄膜。研究内容证实了，酶法改性明胶具有可生物降解性，且由其所制备的产物可生物降解性也较好。

关键词：皮革工艺铬鞣革微生物转谷氨酰胺酶胶原水解产物力学性能酶法改性

Heterotrophic ammonium removal characteristics of an aerobic heterotrophic nitrifying-denitrifying bacterium, Providencia rettgeri YL

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Journal of Environmental Sciences 2009年第10期21卷 1336-1341页

作者： taylor Shauna m School of Environmental Science and Engineering Shanghai Jiao Tong University

Bacterium Providencia rettgeri YL was found to exhibit an unusual ability to heterotrophically nitrify and aerobically denitrify various concentrations of ammonium （NH4^＋-N）. In order to further understand its removal ability, several experiments were conducted to identify the growth and ammonium removal response at different carbon to nitrogen （C/N） mass ratios, shaking speeds, temperatures, ammonium concentrations and to qualitatively verify the production of nitrogen gas using gas chromatography techniques. Results showed that under optimum conditions （C/N 10, 30℃, 120 r/min）, YL can significantly remove low and high concentrations of ammonium within 12 to 48 h of growth, respectively. The nitrification products hydroxylamine （NHzOH）, nitrite （NO2^-） and nitrate （NO3^-） as well as the denitrification product, nitrogen gas （N2）, were detected under completely aerobic conditions.

关键词： Providencia rettgeri YL heterotrophic ammonium removal aerobic denitrification

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工业胰蛋白酶用于处理蓝矾破碎片

明胶科学与技术 2002年第2期22卷 101-101页

作者： taylor,m.m

Host RNA circles and the origin of hepatitis delta virus

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World Journal of Gastroenterology 2014年第11期20卷 2971-2978页

作者： John m taylor Fox Chase Cancer Center PhiladelphiaPA 19111United States

Recent reports show that many cellular RNAs are processed to form circular species that are relatively abundant and resistant to host *** some cases,such circles actually bind host *** depletion of available microRNAs appears to have biological roles;for instance,in homeostasis and *** findings regarding host RNA circles support a speculative reappraisal of the origin and mode of replication of hepatitis delta virus,hepatitis delta virus(HDV),an agent with a small circular RNA genome;specifically,it is proposed that in hepatocytes infected with hepatitis B virus(HBV),some viral RNA species are processed to circular forms,which by a series of chance events lead to an RNA that can be both replicated by host enzymes and assembled,using HBV envelope proteins,to form particles some of which are *** a model also may provide some new insights into the potential pathogenic potential of HDV *** return,new insights into HDV might provide information leading to a better understanding of the roles of the host RNA circles.

关键词： Hepatitis B virus Hepatitis delta virus Circular RNA Ribozymes microRNA

Virus entry mediated by hepatitis B virus envelope proteins

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World Journal of Gastroenterology 2013年第40期19卷 6730-6734页

作者： John m taylor Fox Chase Cancer Center PhiladelphiaPA19111United States

Hepatitis B virus(HBV),a major cause of human liver disease worldwide,encodes three envelope proteins needed for the attachment and entry of the virus into susceptible host cells.A second virus,hepatitis delta virus,which is known to enhance liver disease in HBV infected patients,diverts the same HBV envelope proteins to achieve its own assembly and *** the lab,lentiviral vectors based on human immunodeficiency virus type 1 can be assembled using the HBV envelope proteins,and will similarly infect susceptible *** article provides a partial review and some personal reflections of how these three viruses infect and of how recipient cells become susceptible,along with some consideration of questions that remain to be answered.

关键词： Hepatitis B virus Hepatitis delta virus Receptor Envelope proteins Entry

Synchronization of Weighted Essentially Non-Oscillatory methods

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Communications in Computational Physics 2008年第6期4卷 56-71页

作者： Ellen m.taylor m.Pino martin Department of mechanical and Aerospace Engineering Princeton UniversityPrincetonNJ 08544USA

Weighted essentially non-oscillatory(WENO)methods have been developed to simultaneously provide robust shock-capturing in compressible fluid flow and avoid excessive damping of fine-scale flow features such as *** certain conditions in compressible turbulence,however,numerical dissipation remains unacceptably high even after optimization of the linear component that dominates in smooth *** the nonlinear error that remains,we demonstrate that a large fraction is generated by a“synchronization deficiency”that interferes with the expression of theoretically predicted numerical performance characteristics when the WENO adaptation mechanism is *** deficiency is illustrated numerically in simulations of a linearly advected sinusoidal wave and the Shu-Osher problem[***.,83(1989),pp.32-78].It is shown that attempting to correct this deficiency through forcible synchronization results in violation of *** conclude that,for the given choice of candidate stencils,the synchronization deficiency cannot be adequately resolved under the current WENO smoothness measurement technique.

关键词： Direct numerical simulation large eddy simulation compressible turbulence shock capturing.

Common approach to managing lower urinary tract symptoms and erectile dysfunction

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Asian Journal of Andrology 2008年第1期10卷 45-53页

作者： Jennifer m. taylor Rowena DeSouza Run Wang Division of Urology University of Texas Medical School at Houston Houston TX 77030 USA Department of Urology University of Texas MD Anderson Cancer Center Houston TX 77030 USA

The present paper serves as a review of the associations between lower urinary tract symptoms （LUTS） and erectile dysfunction （ED）, with a focus on common and combined pathways for treatment. LUTS and ED are common conditions seen in general urologic practice. Research has started to establish epidemiologic and pathophysiologic links between the two conditions and a strong association confirmed across multiple studies. men seeking care for one condition should always be interviewed for complaints of the other condition. Proposed common pathways include α-1 adrenergic receptor imbalance, Rho-kinase overactivity, endothelial cell dysfunction and atherosclerosis-induced ischemia. medical therapy has replaced surgery as the first-line treatment for LUTS in most patients, with the incorporation of α-adrenergic receptor antagonists （α-ARAs） and 5-α-reductase inhibitors （5-ARIs） into everyday practice. Treatment with α-ARAs contributes to some improvement in ED, whereas use of 5-ARIs results in worsened sexual function in some patients. Phosphodiesterase-5 （PDE-5） inhibitors have revolutionized the treatment of ED with a simple oral regimen, and new insights demonstrate a benefit of combined use of PDE-5 inhibitors and α-ARAs. The mechanisms of action of these medications support these observed benefits, and they are being studied in the basic science and clinical settings. In addition, novel mechanisms for therapy have been proposed based on clinical and research observations. The minimally invasive and surgical treatments for LUTS are known to have adverse effects on ejaculatory function, while their effects on erectile function are still debated. much remains to be investigated, but it is clear that the associations between LUTS and ED lay the foundation for future therapies and possible preventative strategies.

关键词： erectile dysfunction lower urinary tract symptoms benign prostatic hyperplasia medical therapy

The Representation of Soil moisture−Atmosphere Feedbacks across the Tibetan Plateau in CmIP6

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Advances in Atmospheric Sciences 2023年第11期40卷 2063-2081页

作者： Joshua TALIB Omar V.mÜLLER Emma J.BARTON Christopher m.taylor Pier Luigi VIDALE U.K.Centre of Ecology and Hydrology WallingfordOX108BBU.K. National Centre for Atmospheric Science ReadingRG66BBU.K. Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET) Centro de Estudios de Variabilidad y Cambio Climático(CEVARCAM)Facultad de Ingeniería y Ciencias HídricasUniversidad Nacional del LitoralSanta FeArgentina National Centre for Earth Observation WallingfordOX108BBU.K. Department of meteorology University of ReadingReadingRG66BBU.K.

Thermal processes on the Tibetan Plateau(TP)influence atmospheric conditions on regional and global *** this,previous work has shown that soil moisture−driven surface flux variations feed back onto the *** soil moisture is a source of atmospheric predictability,no study has evaluated soil moisture−atmosphere coupling on the TP in general circulation models(GCms).In this study,we use several analysis techniques to assess soil moisture−atmosphere coupling in CmIP6 simulations including:instantaneous coupling indices;analysis of flux and atmospheric behaviour during dry spells;and a quantification of the preference for convection over drier *** these metrics we partition feedbacks into their atmospheric and terrestrial *** with previous global studies,we conclude substantial inter-model differences in the representation of soil moisture−atmosphere coupling,and that most models underestimate such *** on dry spell analysis,most models underestimate increased sensible heat during periods of rainfall *** example,the model-mean bias in anomalous sensible heat flux is 10 W m−2(≈25%)smaller compared to *** dry-spell sensible heat fluxes lead to a weaker atmospheric *** also find that most GCms fail to capture the negative feedback between soil moisture and deep *** poor simulation of feedbacks in CmIP6 experiments suggests that forecast models also struggle to exploit soil moisture−driven *** improve the representation of land−atmosphere feedbacks requires developments in not only atmospheric modelling,but also surface processes,as we find weak relationships between rainfall biases and coupling indexes.

关键词： model evaluation land-atmosphere feedbacks Tibetan Plateau precipitation surface energy balance