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Biochar-Bacillus consortium for a sustainable agriculture:physicochemical and soil stability analyses

Biochar 2023年第1期5卷 319-333页

作者： Aaronn Avit Ajeng Rosazlin Abdullah tau chuan ling Institute of Biological Sciences Faculty of ScienceUniversity of Malaya50603 Kuala LumpurMalaysia Centre for Research in Biotechnology for Agriculture(CEBAR) Institute If Biological SciencesFaculty of ScienceUniversity of Malaya50603 Kuala LumpurMalaysia

Biochar has various agricultural applications,including the promising use as a carrier for beneficial ***,most recent research has demonstrated the possible attachment or immobilization of a single bacterial species onto biochar rather than a consortium of microbes for biotechnological ***,an assessment on the potential of oil palm kernel shell(OPKS)biochar as a biofilm-producing Bacillus consortium carrier through optimization study on the operating and environmental factors influencing the biofilm adhesion was conducted using response surface methodology(RSM)and the subsequent soil stability and storage potential of the *** highest Bacillus population was observed at temperature 33°C,agitation speed of 135 rpm,at a neutral pH of 7.5 with 10%(w/w)of sago starch as the co-carbon *** adhesion of Bacillus on OPKS biochar following the optimized conditions fitted pseudo-second order(PSO)of kinetic modelling(R^(2)=0.998).The optimized formulation was subjected to storage in different temperatures and in vitro soil incubation which revealed that the Bacillus biofilmadhered OPKS biochar may be stored up to 4 months with minimum range of live Bacillus viability reaching 107 CFU g^(-1) of biochar which is within the minimum range of acceptable biofertilizer viability(106 CFU mL^(-1)).Formulation that is viable in room storage can be easily incorporated into current agricultural distribution networks that do not have *** work highlighted the physicochemical and soil stability qualities of optimized Bacillus consortium adhesion on biochar for agricultural usage.

关键词： Process optimization Biochar Plant growth promoting bacteria Adhesion kinetics And Isotherms

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Raman spectroscopy as process analytical tool in downstream processing of biotechnology

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Chinese Journal of Chemical Engineering 2021年第2期34卷 204-211页

作者： Yu Kiat Lin Hui Yi Leong tau chuan ling Dong-Qiang Lin Shan-Jing Yao Key Laboratory of Biomass Chemical Engineering of Ministry of Education College of Chemical and Biological EngineeringZhejiang UniversityHangzhou 310027China Institute of Biological Sciences Faculty of ScienceUniversity of MalayaKuala Lumpur 50603Malaysia

Downstream processing or product recovery plays a vital role in the development of *** improve the bioprocess efficiency,some unconventional methods are much *** continuous manufacturing in downstream processing makes the Process Analytical Technologies(PATs)as an important *** and controlling bioprocess are an essential factor for the principles of PAT and quality by *** methods can apply to monitor multiple analytes in real-time with less sample processing with significant *** spectroscopy is an extensively used technique as an analytical and research tool owing to its modest process form,non-destructive,non-invasive optical molecular spectroscopic imaging with computer-based ***,its application is essential for the analysis and characterization of biological samples,and it is easy to operate with minimal *** innovation on various types of enhanced Raman spectroscopy was designed to enhance the Raman analytical *** spectroscopy could couple with chemometrics to provide reliable alternative analysis method of downstream process ***,this review aims to provide useful insight on the application of Raman spectroscopy for PAT in downstream processing of biotechnology and Raman data analysis in biological fields.

关键词： Raman spectroscopy Process analytical technology Downstream bioprocessing Biotechnology

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Sonication and grinding pre-treatments on Gelidium amansii seaweed for the extraction and characterization of Agarose

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Frontiers of Environmental Science & Engineering 2018年第4期12卷 17-23页

作者： Kit Wayne Chew Pau Loke Show Yee Jiun Yap Joon Ching Juan Siew Moi Phang tau chuan ling Jo-Shu Chang Department of Chemical and Environmental Engineering Faculty of Engineering University of Nottingham Malaysia Campus Jalan Broga 43500 Semenyih Selangor Darul Ehsan Malaysia Bioseparation Research Group Faculty of Engineering University of Nottingham Malaysia Campus Jalan Broga 43500 Semenyih Selangor Darul Ehsan Malaysia Department of Applied Mathematics Faculty of Engineering University of Nottingham Malaysia Campus Jalan Broga 43500 Semenyih Selangor Darul Ehsan Malaysia Nanotechnology & Catalysis Research Centre (NANOCAT) Institute of Postgraduate Studies University of Malaya 50603 Kuala Lumpur Malaysia Institute of Ocean and Earth Sciences University of Malaya 50603 Kuala Lumpur Malaysia Institute of Biological Sciences University of Malaya 50603 Kuala Lumpur Malaysia Department of Chemical Engineering National Cheng Kung University Tainan 701 Taiwan China

Various prctreatments methods including sonication and grinding were perfonned on red seaweed Gelidium amansii for the subsequent extraction ofagarose. The agarose products are usually extracted from agar powder products from seaweeds. In this study, the agarose was extracted using a direct polyethylene glycol （PEG） method without the need to first process the agar from seaweed. The agar extract was frozen then thawed and mixed directly with PEG solution to precipitate the agarose. The quality of agarose obtained was evaluated through physico-chemical properties analysis which includes spectral technique （FTIR）, melting and boiling point, gel strength and sulfate content. These properties were compared with a non-pretreated sample and it was found that the addition of pretreatment steps improved the quality of agarosc but gave a slightly lower yield. The gel strength of pretreated samples was much higher and the sulfate content was lower compared to non-pretreated samples. The best pretreatment method was sonication which gave gel strength of 742 gcm - and sulfate content of 0.63%. The extraction of agarose can be further improved with the use of different neutralizing agents. Pretreating the seaweed shows potential in improving the quality of agarosc from seaweed and can be applied for thture extraction of the agarose.

关键词： AgaroseGe lidium Pretreatment Seaweed Sonication

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Bioremediation strategies of palm oil mill effluent and landfill leachate using microalgae cultivation:An approach contributing towards environmental sustainability

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Chinese Chemical Letters 2023年第5期34卷 57-71页

作者： Imran Ahmad Natasha Nabila Binti Ibrahim Norhayati Abdullah Iwamoto Koji Shaza Eva Mohamad Kuan Shiong Khoo Wai Yan Cheah tau chuan ling Pau Loke Show Algae and Biomass Research Laboratory Malaysia-Japan International Institute of Technology(MJIIT)Universiti Teknologi Malaysia(UTM)Jalan Sultan Yahya Petra54100Kuala LumpurMalaysia Department of Chemical and Environmental Engineering Faculty of Science and EngineeringUniversity of Nottingham MalaysiaJalan BrogaSemenyih 43500Selangor Darul EhsanMalaysia Centre of Research in Development Social and Environment(SEEDS)Faculty of Social Sciences and HumanitiesUniversiti Kebangsaan Malaysia43600 UKM BangiSelangor Darul EhsanMalaysia Institute of Biological Sciences Faculty of ScienceUniversity of Malaya50603Kuala LumpurMalaysia Zhejiang Provincial Key Laboratory for Subtropical Water Environment and Marine Biological Resources Protection Wenzhou UniversityWenzhou 325035China Department of Sustainable Engineering Saveetha School of EngineeringSIMATSChennai 602105India

Palm oil mill effluent(POME)is defined as the wastewater that contains high concentrations of organics,nutrients and oil and grease generated from the production process of palm ***,proper discharge and management of POME is important to avoid deleterious impact on the *** fact,solid waste generation is a precursor for its disposal issues as most of the solid waste generated in developing nations is dumped into *** has led to the threat posed by the generation of landfill leachate(LL).LL is a complex dark coloured liquid consisting of organic matter,inorganic substances,trace elements and ***,it is essential to effectively treat the landfill leachate before discharging it to avoid contamination of soil,surface&groundwater *** treatment methods comprises of physical,biological and chemical treatment,however,microalgal-based treatment could also be ***,with the benefits offered by microalgae in valorisation,the application of microalgae in POME and leachate treatment as well as biofuel production,is considerably *** paper provides an acumen of the microalgae-based treatment of POME and LL,integrated with biofuel production in a systematic and critical *** pollutants assimilation from wastewater and CO_(2)biosequestration are discussed for environmental *** systems for wastewater treatment with simultaneous biomass production and its valorisation,are *** study aims to provide insight to industrial stakeholders on economically viable and environmentally sustainable treatment of wastewaters using microalgae,and eventually contributing to the circular bioeconomy and environmental sustainability.

关键词： Microalgae Biofuel Landfill leachate Palm oil mill effluent Treatment Sustainability

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Multiwavelength Observations of the Infrared Dust Bubble N75 and its Surroundings

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Research in Astronomy and Astrophysics 2024年第4期24卷 262-270页

作者： Quan-ling Cui chuan-Peng Zhang Jun-Jie Wang National Astronomical Observatories Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China College of Science Qiqihar UniversityQiqihar 161006China Guizhou Radio Astronomical Observatory Guizhou UniversityGuiyang 550000China

Infrared dust bubbles play an important role in the study of star formation and the evolution of the interstellar *** this work,we study the infrared dust bubble N75 and the infrared dark cloud G38.93 mainly using the tracers C^(18)O,HCO^(+),HNC and N_(2)H^(+)observed by the 30 m IRAM *** also study the targets using data from large-scale surveys:GLIMPSE,MIPSGAL,GRS,NRAO VLA Sky Survey and Bolocam Galactic Plane *** found that the C^(18)O emission is morphologically similar to the Spitzer IRAC 8.0μm ***1.1 mm cold dust emission of G38.93 shows an elongated structure from southwest to *** ionized gas from G38.93 is surrounded by polycyclic aromatic hydrocarbon emission,which may be excited by radiation from *** found that the identified young stellar objects tend to cluster around G38.93 and are mostly in class II with several class I cases distributed around N75,but no class II *** also found evidence of expanding feedback,which could have triggered star formation.

关键词： ISM:bubbles infrared:ISM galaxies:star formation

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Possible Habitats for NH_(3),NH_(2)D,H^(13)CN,HC^(15)N,SO,and C^(18)O in the Initial Conditions of High-mass Star Formation

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Research in Astronomy and Astrophysics 2024年第4期24卷 237-252页

The initial condition of high-mass star formation is a complex area of study because of the high densities(n_(H_(2))>106cm^(-3))and low temperatures(T_(dust)<18 K)*** such conditions,many molecules become depleted from the gas phase by freezing out onto dust ***,the N-bearing and deuterated species could remain gaseous under these extreme conditions,suggesting that they may serve as ideal *** this paper,using the Plateau de Bure Interferometer and Very Large Array observations at 1.3 mm,3.5 mm,and 1.3 cm,we investigate the possible habitats for NH_(3),NH_(2)D,H^(13)CN,HC^(15)N,SO,and C^(18)O in eight massive precluster and protocluster clumps G18.17,G18.21,G23.97N,G23.98,G23.44,G23.97S,G25.38,and *** found that the NH3cores are in good agreement with the 3.5 mm peak emission,but the NH_(3)is much more extended than the 3.5 mm emission *** SO distributions agree well with the 3.5 mm peaks for the evolved star formation stage,but we did not detect any SO emission in the four earliest star formation sources.C^(18)O is a poor tracer in conditions of the cold(■18 K)and dense(■10^(4)cm^(-3))cores,e.g.,the prestellar *** also found that the NH_(2)D cores are mainly located in the temperature range of 13.0-20.0 K,and the NH_(2)D lines may be strongly depleted above 20 K.

关键词： galaxies:star formation techniques:interferometric methods:observational

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DEM analysis of the influence of stirrer design on die filling with forced powder feeding

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Particuology 2024年第5期88卷 107-115页

作者： Chao Zheng Edward Yost Ariel R.Muliadi Nicolin Govender ling Zhang chuan-Yu Wu School of Chemistry and Chemical Engineering University of SurreyGuildfordGU2 7XHUnited Kingdom Small Molecule Pharmaceutics GenentechInc.One DNA WaySouth San FranciscoCA94080USA

Die filling is a critical stage during powder compaction,which can significantly affect the product quality and *** this paper,a forced feeder is introduced attempting to improve the filling performance of a lab-scale die filling *** die filling process is analysed with a graphics processing units(GPU)enhanced discrete element method(DEM).Various stirrer designs are assessed for a wide range of process settings(i.e.,stirrer speed,filling speed)to explore their influence on the die filling performance of free-flowing *** results show that die filing with the novel helical-ribbon(i.e.,type D)stirrer design exhibits the highest filling ratio,implying that it is the most robust stirrer design for the feeder configuration ***,die filling performance with the type D stirrer design is a function of the stirrer speed and the filling speed.A positive variation of filling ratio(ηf>0%)can be ensured over the whole range of filling speed by adjusting the stirrer speed(i.e.,increasing the stirrer speed).The approach used in this study can not only help understand how the stirrer design affects the die filling performance but also guide the optimization of feeder system and process settings.

关键词： Discrete element method Die filling Forced feeding Stirrer design GPU computing

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A mixed-valence polyoxometalate-based 3D inorganic framework cathode material for high-efficiency rechargeable AZIBs

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Rare Metals 2024年第8期43卷 3677-3691页

作者： Qing Han Hao-Ran Xiao Tao Zhou Bing-chuan Li Liu Yang ling-ling Xie Xue-Jing Qiu Xian-Yong Wu Li-Min Zhu Xiao-Yu Cao School of Chemistry and Chemical Engineering Henan University of TechnologyZhengzhou 450001China Key Laboratory of High Specific Energy Materials for Electrochemical Power Sources of Zhengzhou City Zhengzhou 450001China College of Materials Engineering Henan University of EngineeringZhengzhou450007China School of Environmental Engineering Henan University of TechnologyZhengzhou 450001China Department of Chemistry University of Puerto Rico-Rio Piedras CampusSan JuanPR 00925USA

The global trend towards new energy storage systems has stimulated the development of electrochemical energy storage *** these technologies,rechargeable aqueous zinc-ion batteries(AZIBs)have attracted considerable interest as a potential alternative to lithium-ion batteries(LIBs)due to their affordable cost,environmental compatibility and high safety *** this study,a high-quality electrode for AZIBs has been successfully developed using a dehydrated mixed-valence polyoxometalate-based three-dimensional(3D)inorganic framework material known as[H_6Mn_(3)V^Ⅳ_(15)V-^Ⅴ_(4O)_(46)(H_2O)_(12)](3D-MnVO).This innovative 3D-MnVO material is built from the alternate connections of{V_(19)O_(46)}"sphere-shaped"clusters andμ_(2)-{Mn(H_(2)O)_(4)}bridges,where each{V_(19)O_(46)}cluster is surrounded by three pairs of vertically distributed{Mn(H_(2)O)_(4)}units,thus resulting in the 3D interpenetrating grid-like network from the infinite[-{V_(19)O_(46)}-μ_(2)-Mn(H_(2)O)_(4)-{V_(19)O_(46)}]_∞chains in three mutually perpendicular *** 3D framework structure of 3D-MnVO possesses abundant oxygen vacancies,spacious and multi-level interconnected channels for ion transport,which facilitates the efficient intercalation/deintercalation of hydrated Zn^(2+)into the pores of the primary structure via the intercalation capacitance *** a result,the 3D-MnVO electrode exhibits excellent diffusion rates and minimal interfacial *** a current density of 0.1 A·g^(-1),the 3D-MnVO cathode delivers a commendable discharge capacity of170.5 mAh·g^(-1)with 81.6%capacity retention after100 charge/discharge ***,even at a high current density of 1.0 A·g^(-1),the 3D-MnVO electrode delivers a remarkable reversible capacity of198.9 mAh·g^(-1).Our research results provide valuable insights into the development of advanced polyoxometalate-based 3D inorganic framework electrode materials for high-performance rechargeable AZIBs.

关键词： Cathode Polyoxometalate 3D inorganic framework Zinc-ion batteries Energy storage mechanism

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Glaucoma drainage device implantation and cyclophotocoagulation in the management of refractory glaucoma after Descemet-stripping automated endothelial keratoplasty

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International Journal of Ophthalmology(English edition) 2024年第2期17卷 257-264页

作者： Min-Shu Wang Xue-chuan Dong Mi-Yun Zheng Xiang Fan Ge-Ge Xiao Jing Hong ling-ling Wu Department of Ophthalmology Peking University Third HospitalBeijing 100191China Key Laboratory of Restoration of Damaged Ocular Nerve Beijing 100191China NIMO Ophthalmology Research Institute Beijing 100176China Department of Ophthalmology the First Hospital of PutianTeaching HospitalFujian Medical UniversityPutian 351100Fujian ProvinceChina

AIM:To compare the surgical outcomes of glaucoma drainage device implantation(GDI)and trans-scleral neodymium:YAG cyclophotocoagulation(CPC)in the management of refractory glaucoma after Descemetstripping automated endothelial keratoplasty(DSAEK).METHODS:This retrospective study on observational case series enrolled 29 patients who underwent DSAEK and posterior anti-glaucoma surgery(15 with GDI and 14 with CPC).The main outcome measures were intraocular pressure(IOP),glaucoma surgery success rate(defined as IOP of 6–21 mm Hg without additional anti-glaucoma operation),number of glaucoma medications,endothelial graft status,and best-corrected visual acuity(BCVA).RESULTS:The mean follow-up time was 34.1 and 21.0mo for DSAEK or glaucoma surgeries,both for the GDI and CPC *** groups showed significant IOP reduction after glaucoma *** GDI group presented a significantly higher success rate in IOP control than the CPC group(60%vs 21.4%,P=0.03).Both procedures significantly decreased the number of glaucoma medications(P=0.03).Forty percent and 57%of cases in the GDI and the CPC group,respectively,experienced endothelial graft failure during follow-up(P=0.36).Significantly worse BCVA after surgery was observed in the CPC group but not in the GDI ***:Both GDI and CPC significantly decrease IOP in eyes with glaucoma after *** is preferable to CPC in refractory glaucoma cases after DSAEK,as it manifests a significantly higher success rate for IOP control,similar endothelial graft failure rate,and relatively preserves BCVA than CPC.

关键词： glaucoma drainage device implantation cyclophotocoagulation refractory glaucoma Descemetstripping automated endothelial keratoplasty

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Intracellular redox potential-driven live-cell synthesis of CdSe quantum dots in Staphylococcus aureus

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Science China Chemistry 2024年第3期67卷 990-999页

作者： Cai-Qin Wu An-An Liu Xu Li Jia-Wei Tu Juan Kong ling-ling Yang Jian-Hong Jia chuan Wang Bin Hu Zhi-Xiong Xie Dai-Wen Pang The Institute for Advanced Studies College of Chemistry and Molecular SciencesCollege of Life SciencesHubei Key Laboratory of Cell HomeostasisWuhan UniversityWuhan 430072China Research Center for Analytical Sciences College of ChemistryState Key Laboratory of Medicinal Chemical BiologyTianjin Key Laboratory of Biosensing and Molecular RecognitionFrontiers Science Center for New Organic MatterFrontiers Science Center for Cell Responsesand Haihe Laboratory of Sustainable Chemical TransformationsNankai UniversityTianjin 300071China

Biosynthesized semiconductor quantum dots(QDs) have bright fluorescence,adjustable particle sizes,and environmental friendliness,endowing them with convenience and potential for biological *** to the unclear mechanism of the cellular environment on the live-cell synthesis of QDs,it is still difficult to regulate their optical ***,the critical role of the intracellular redox environment in regulating the fluorescence properties of biosynthesized CdSe QDs in Staphylococcus aureus(***) has been *** glutathione peroxidase(GPx) activity directly affects the intracellular H_(2)O_(2) produced in the SeO_(3)^(2-) reduction,which further manipulates the glutathione redox cycle to determine the content of low-valence *** a result,the fluorescence intensity of the synthesized CdSe QDs increases by 60% in the GPx overexpressed *** cellular redox potential that is controlled by the GSH redox cycle provides the driving force for the reduction of SeO_(3)^(2-),facilitating the synthesis of CdSe QDs in *** *** proposed mechanism of the cellular redox state provides a new perspective for regulating the synthesis of semiconductor nanomaterials in live cells.

关键词： redox H_(2)O_(2) peroxidase quantum dot Staphylococcus aureus

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