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Benefits of Ilizarov automated bone distraction for nerves and articular cartilage in experimental leg lengthening

World Journal of Orthopedics 2017年第9期8卷 688-696页

作者： Nathalia Shchudlo tatyana varsegova tatyana Stupina Michael Shchudlo Marat Saifutdinov Andrey Yemanov Laboratory of Morphology FSBI Russian Ilizarov Scientific Center "Restorative Traumatology and Orthopaedics"

AIM To determine peculiarities of tissue responses to manual and automated Ilizarov bone distraction in nerves and articular *** Twenty-nine dogs were divided in two experimental groups: Group M-leg lengthening with manual distraction(1 mm/d in 4 steps), Group A-automated distraction(1 mm/d in 60 steps) and intact group. Animals were euthanized at the end of distraction, at 30 th day of fixation in apparatus and 30 d after the fixator removal. M-responses in gastrocnemius and tibialis anterior muscles were recorded, numerical histology of peronealand tibialis nerves and knee cartilage semi-thin sections, scanning electron microscopy and X-ray electron probe microanalysis were *** Better restoration of M-response amplitudes in leg muscles was noted in A-group. Fibrosis of epineurium with adipocytes loss in peroneal nerve, subperineurial edema and fibrosis of endoneurium in some fascicles of both nerves were noted only in M-group, shares of nerve fibers with atrophic and degenerative changes were bigger in M-group than in A-group. At the end of experiment morphometric parameters of nerve fibers in peroneal nerve were comparable with intact nerve only in A-group. Quantitative parameters of articular cartilage(thickness, volumetric densities of chondrocytes, percentages of isogenic clusters and empty cellular lacunas, contents of sulfur and calcium) were badly changed in M-group and less changed in *** Automated Ilizarov distraction is more safe method of orthopedic leg lengthening than manual distraction in points of nervous fibers survival and articular cartilage arthrotic changes.

关键词： Limb lengthening Articular cartilage Nerve Histomorphometry Dog

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Assessment of palmar subcutaneous tissue vascularization in patients with Dupuytren's contracture

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World Journal of Orthopedics 2018年第9期9卷 130-137页

作者： Nathalia Shchudlo tatyana varsegova tatyana Stupina Tamara Dolganova Michael Shchudlo Nathalia Shihaleva Vadim Kostin Clinics and Experimental Laboratory for Reconstructive Microsurgery and Hand Surgery Russian Ilizarov Scientific Center "Restorative Traumatology and Orthopaedics" Laboratory of Morphology of Federal State Budget Institution Russian Ilizarov Scientific Center "Restorative Traumatology and Orthopaedics"

AIM To investigate the structural and functional characte-ristics of palmar hypodermal tissue vascularization in Dupuytren's contracture patients of different *** Eighty-seven Dupuytren's contracture patients underwent partial fasciectomy. Twenty-two of them were less than 55 years old(Y-group, n = 22); the others were 55 and older(O-group, n = 65). In surgically excised representative tissue samples, a histomorphometric analysis of the perforating arteries of the palmar aponeurosis and stereologic analysis of hypodermis vascularity were performed. The method of laser flowmetry estimated the microcirculation of the skin of the *** Frequency of cases with rapid development of contracture(less than 5 years) was 13.6% in the Y-group and 40% in the O-group, P < 0.05. The external and luminal diameters of perforating arteries in palmar fascia were decreased more severely in Y. The thickness of intima increased three times compared with healthy control, and the intima/media relation also increased, especially in O. Increased numerical and volumetric micro-vessel densities in hypodermis, percentage of large vessels(more than 12 μm in diameter), and percentage of vessels with signs of periadventitial inflammatory infiltration were noted in Y. The percentage of vessels with adventitial fibrosis was greater in O than in Y. Base capillary flow in Y was increased compared to healthy control subjects and to O, and peak capillary flow was increased in comparison with *** Compared to the O-group, Y-group patients exhibited more severe constrictive remodeling of palmar fascia perforating arteries supplying hypodermis but more expressed compensatory changes of its capillarization.

关键词： Dupuytren’s contracture Laser Doppler flowmetry Hypodermis Histo-morphometry Palmar fascia

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Three-Dimensional Convection in an Inclined Porous Layer Subjected to a Vertical Temperature Gradient

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Fluid Dynamics & Materials Processing 2024年第9期20卷 1957-1970页

作者： Ivan Shubenkov tatyana Lyubimova Evgeny Sadilov Computational Fluid Dynamics Laboratory Institute of Continuous Media Mechanics UB RASPerm614068Russia Theoretical Physics Department Perm State UniversityPerm614990Russia

In this paper,we study the onset and development of three-dimensional convection in a tilted porous layer saturated with a *** layer is subjected to a gravitational field and a strictly vertical temperature ***,problems of thermal convection in tilted porous media saturated with a liquid are studied by assuming constant different temperatures at the boundaries of the layer,which prevent these systems from supporting conductive(non-convective)*** boundary conditions considered in the present work allow a conductive state and are representative of typical geological *** an earlier work,we carried out a linear stability analysis of the conductive *** was shown that at any layer tilt angles,the most dangerous type of disturbances are longitudinal ***,a non-zero velocity component exists in *** the present work,threedimensional non-linear convection regimes are *** original three-dimensional problem is reduced to two-dimensional one with an analytical expression for the velocity z-component v_(z)=v_(z)(x,y).It is shown that the critical Rayleigh number values obtained through numerical solutions of the obtained 2D problem by a finite difference method for different layer inclination angles,are in a good agreement with those predicted by the linear *** number of convective rolls realized in nonlinear calculations also fits the linear theory predictions for a given cavity *** carried out at low supercriticalities show that a direct bifurcation takes *** increasing supercriticality,no transitions to other convective regimes are *** situation studied in this problem can be observed in oil-bearing rock formations under the influence of a geothermal temperature gradient,where the ensuing fluid convection can affect the distribution of oil throughout the layer.

关键词： Thermal convection inclined layer porous media vertical temperature gradient

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Investigation of Cavitation in NaCl Solutions in a Sonochemical Reactor Using the Foil Test Method

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Fluid Dynamics & Materials Processing 2024年第5期20卷 1093-1102页

作者： Michael Kuchinskiy tatyana Lyubimova Konstantin Rybkin Anastasiia Sadovnikova Vasiliy Galishevskiy Institute of Continuous Media Mechanics UB RAS Perm614013Russia Faculty of Physics Perm State UniversityPerm614068Russia

Ultrasonic baths and sonochemical reactors are widely used in industrial applications dealing with surface cleaningand chemical synthesis. The processes of erosion, cleaning and structuring of the surface can be typically controlledby changing relevant influential parameters. In particular, in this work, we experimentally investigate theeffect of NaCl concentration (0–5.5 mol/L) on the erosion of an aluminum foil under ultrasonic exposure at afrequency of 28 kHz. Special attention is paid to the determination of cavitation zones and their visualizationusing heat maps. It is found that at low NaCl concentration (0.3 mol/L), the foil destruction rate is higher thanin distilled water. At higher concentrations of salt, cavitation takes place mainly in the upper part of the container.

关键词： Ultrasound NaCl solution foil test cavitation activity

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Formation of Water Quality of Surface Water Bodies Used in the Material Processing

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Fluid Dynamics & Materials Processing 2024年第4期20卷 815-828页

作者： tatyana Lyubimova Anatoly Lepikhin Yanina Parshakova Irina Zayakina Alibek Issakhov Laboratory of Computational Fluid Dynamics Laboratory Institute of Continuous Media Mechanics UB RASPerm614068Russia Laboratory of Land Hydrology Problems Mining Institute UB RASPerm614000Russia Department of Theoretical Physics Perm State UniversityPerm614990Russia Department of Mathematical and Computer Modeling Al-Farabi Kazakh National UniversityAlmaty050040Republic of Kazakhstan

In the process of production or processing of materials by various methods,there is a need for a large volume of water of the required *** in many regions of the world,there is an acute problem of providing industry with water of a required *** solution is an urgent and difficult *** water quality of surface water bodies is formed by a combination of a large number of both natural and anthropogenic factors,and is often significantly heterogeneous not only in the water area,but also in *** a rule,the water supply of large industrial enterprises is located along the river *** are the most important nodes of river *** the mechanism of transport of pollutants at the confluence of rivers is critical for assessing water *** recent years,thanks to the data of satellite images,the interest of researchers in the phenomenon of mixing the waters of merging rivers has *** nature of the merger is influenced by the formation of transverse *** the framework of this work,a study of vorticity,as well as the width of the mixing zone,depending on the distance from the confluence,the speeds of the merging rivers and the angle of confluence was carried *** the consumer properties of water are largely determined by its chemical and physical indicators,the intensity of mixing,determined largely by the nature of the secondary circulation,is of fundamental importance for assessing the distribution of hydrochemical indicators of water quality in the mixing *** characteristics are important not only for organizing water intake for drinking and technical purposes with the best consumer properties,but also for organizing an effective monitoring system for confluence zones.

关键词： Water for material processing water quality formation of transverse circulation

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A Numerical Investigation of the Effect of Boundary Conditions on Acoustic Pressure Distribution in a Sonochemical Reactor Chamber

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Fluid Dynamics & Materials Processing 2024年第6期20卷 1425-1439页

作者： Ivan Sboev tatyana Lyubimova Konstantin Rybkin Michael Kuchinskiy UEC-Aviadvigatel 93 Komsomolsky ProspectPerm614990Russia Laboratory of Computing Hydrodynamic Institute of Continuous Media Mechanics UB RASPerm614013Russia Laboratory of Interfacial Hydrodynamic Perm State UniversityPerm614068Russia

The intensification of physicochemical processes in the sonochemical reactor chamber is widely used in problems of synthesis,extraction and *** of the most important mechanisms at play in such processes is the acoustic cavitation due to the non-uniform distribution of acoustic pressure in the *** has a strong impact on the surface degradation *** this work,a numerical calculation of the acoustic pressure distribution inside the reactor chamber was performed using COMSOL *** numerical results have revealed the dependence of the structure of the acoustic pressure field on the boundary conditions for various thicknesses of the piezoelectric *** particular,the amplitude of the acoustic pressure is minimal in the case of absorbing boundaries,and the attenuation becomes more significant as the thickness of the piezoelectric transducer *** addition,reflective boundaries play a significant role in the formation and distribution of zones of maximum cavitation activity.

关键词： Ultrasound numerical simulation acoustic pressure field cavitation erosion foil test

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Detection and evaluation of different morphological forms of Mycoplasma hominis in human semen

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Asian Journal of Andrology 2024年第1期26卷 71-76页

作者： Elizaveta Bragina tatyana Sorokina Avtandil Chogovadze Yulia Shevchenko Vyacheslav Chernykh Olga Barkhatova Irina Rakovskaya Research Centre for Medical Genetics Moscow 115522Russia A.N.Belozersky Institute of Physico-Chemical Biology Lomonosov Moscow State University Moscow 119992Russia Reprolab Limited Liability Company Moscow 119333Russia N.I.Pirogov Russian National Research Medical University Moscow 117997Russia Federal Government Budgetary Institution"National Research Center for Epidemiology and Microbiology Named after Honorary Academician N.F.Gamaleya of the Ministry of Health of the Russian Federation" Moscow 123098Russia

Recently discovered microcolonial forms of Mycoplasma hominis(***)and their impact on human spermatogenesis are *** spermatozoa of 125 fertile men(sperm donors;from Reprobank[Reproductive Tissue Bank,Moscow,Russial)and of 93 patients with fertility problems(from the Federal State Budgetary Institution"Research Centre for Medical Genetics[RCMG]",Moscow,Russia)were *** colonies of *** and microcolonies were detected by molecular biological methods,culture of bacteria,and transmission electron *** unique structure of microcolonial cells,in which the cytoplasmic cylinder is surrounded by concentric electron-dense and electron-light layers with a periodicity of 12-14 nm,and the ability of microcolonial cells to attach to spermatozoa are *** patients with lower sperm quality,microcolonies of *** were detected 2.5 times more frequently than classical *** detection of microcolonies in the ejaculate and the frequent isolation of microcolonies from sperm samples of patients with fertility problems suggest that microcolonial cells may be one cause of infertility.

关键词： ejaculate fertility Mycoplasma hominis spermatogenesis transmission electron microscopy

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Inpatient management of iron deficiency anemia in pediatric patients with inflammatory bowel disease: A single center experience

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World Journal of Clinical Pediatrics 2024年第1期13卷 107-117页

作者： Krishanth Manokaran Jonathan Spaan Giulio Cataldo Christopher Lyons Paul D Mitchell tatyana Sare LoriA Zimmerman Paul A Rufo Center for Inflammatory Bowel Disease Boston Children's HospitalBostonMA 02115United States Institutional Centers for Clinical and Translational Research Boston Children's HospitalBostonMA 02115United States

BACKGROUND Screening for iron deficiency anemia(IDA)is important in managing pediatric patients with inflammatory bowel disease(IBD).Concerns related to adverse reactions may contribute to a reluctance to prescribe intravenous(IV)iron to treat IDA in this *** To track the efficacy and safety of IV iron therapy in treating IDA in pediatric IBD patients admitted to our *** A longitudinal observational cohort study was performed on 236 consecutive pediatric patients admitted to our tertiary IBD care center between September 2017 and December 2019.92 patients met study criteria for IDA,of which 57 received IV iron,17 received oral iron,and 18 were discharged prior to receiving iron *** Patients treated with IV iron during their hospitalization experienced a significant increase of 1.9(±0.2)g/dL in mean(±SE)hemoglobin(Hb)concentration by the first ambulatory follow-up,compared to patients who received oral iron 0.8(±0.3)g/dL or no iron 0.8(±0.3)g/dL(P=0.03).One out of 57(1.8%)patients that received IV iron therapy experienced an adverse *** Our findings demonstrate that treatment with IV iron therapy is safe and efficacious in improving Hb and iron levels in pediatric patients with IDA and active IBD.

关键词： Iron deficiency anemia Pediatric inflammatory bowel disease Intravenous iron therapy Inflammatory bowel disease

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3,6-Bis-β-Dicarbonylsubstituted Carbazoles Bearing N-Spacers and Their Eu(III) Complexes as Immunofluorescent Labelling Agents

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International Journal of Organic Chemistry 2024年第1期14卷 20-31页

作者： Dmitry E. Pugachev Georgy V. Zatonsky tatyana S. Kostryukova Anna G. Shubina Nikolay V. Vasiliev Department of Biological Microanalysis FGUP State Research Institute of Biological Engineering Moscow Russia Department of Theoretical and Applied Chemistry State University of Education Mytishchi Russia

New reagents for immunofluorescence analysis of carbazole series containing fluorinated β-dicarbonyl fragments and carboxylic substituent groups separated by spacers of different lengths from the light-gathering carbazole scaffold have been developed. The markers in complex with Eu3+ ions possess stability in the aqueous phase, intense and prolonged luminescence (τ 550 - 570 μs) with characteristic emission maxima in the region of 615 nm and excitation wavelengths in the region of 380 - 390 nm, which distinguishes them from most of the analogs used. In the study of marker conjugation with streptavidin, a reagent containing 4 - 5 europium labeling complexes based on spacer-containing carbazole tetraketone was obtained. The marker-doped silicate nanoparticles exhibit intense and long-lived luminescence in the characteristic region.

关键词： Fluorescence Immunoassay Fluorinated β-Diketones Carbazole Europium Complexes Streptavidin Nanodispersions

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Structure and heterogeneity of habitat determine diet of predators despite prey abundance:Similar response in Long-eared,Short-eared Owls and Common Kestrels

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Avian Research 2023年第1期14卷 54-61页

作者： tatyana Kovinka Alexander Sharikov tatyana Massalskaya Sergey Volkov Zoology and Ecology Department Moscow State Pedagogical UniversityKibalchicha Str.6-3129164MoscowRussid Severtsov Institute of Ecology and Evolution Russian Academy of SciencesMoscow119071Russia

According to one of the theses of optimal foraging theory,main prey species abundance in the hunting area is the main factor determining the diet and habitat choices of birds of *** other factors can also be *** habitat structure influences the predators’diets as *** this study we examined the influence of habitat structure on diet compositions of three species of birds:Long-eared Owl(Asio otus),Short-eared Owl(***)and Common Kestrel(Falco tinnunculus).The study was carried out from 2007 to 2019 in a 48 km2area of the Crane’s Homeland Reserve,Moscow Region,*** habitat structures of model species’hunting territories(ratio of different types of landscape elements)were classified in module"Semi-Automatic Classification Plugin"based on the QGIS.A boosted regression tree analysis identified that the share of the main prey species in the diet is primarily determined by the landscape structure of hunting *** largest share of Common Vole(Microtus arvalis)in birds’diet was determined by the shrubs area(15%of hunting area),the meadow area(75%),the habitat heterogeneity(70%)and the arable land area(5%).The same predictors determined the largest share of Root Vole(Microtus oeconomus):the shrubs area 25%,the meadow area 70%,and the arable land area 3%.The annual mean abundance of prey species did not determine their importance in the diet of birds of ***,the main prey abundance in the hunting area is not a determining factor for the formation of diet composition of birds of prey.

关键词： Birds of prey diet Boosted regresion tnee Habitat strnocture efiect Prey sbundance

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