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Aeromonas veronii isolated from climbing perch(Anabas testudineus)suffering from epizootic ulcerative syndrome(EUS)

Aquaculture and Fisheries 2023年第3期8卷 288-295页

作者： Rakib Ehsan Ashikur Rahman Sulav Indra Paul Md.Ali Arman Ador Md.Shameul Haque tasmina akter Md.Mahbubur Rahman Institute of Biotechnology and Genetic Engineering Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur1706Bangladesh Department of Fisheries Management Faculty of FisheriesBangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur1706Bangladesh

Motile Aeromonads are responsible for various diseases in fishes that cause significant economic losses in *** *** also frequently isolated from fish suffering from Epizootic Ulcerative Syndrome(EUS).The present study was conducted to isolate and identify *** from Vietnam climbing perch(Anabas testudineus)suffering from EUS,evaluate the pathogenicity of the isolates,and find out antibiogram profile and susceptibility of the isolates to medicinal plant *** climbing perch(***)suffering from EUS was collected from fish farms located at Savar,Dhaka and bacteria were isolated from the external ulcerative lesion,and kidney and liver of the infected fish on nutrient agar *** phenotypic identification of the bacterial isolates was performed following morphological,physiological and biochemical characterization *** 16S rRNA gene sequencing was accomplished for further molecular identification of five selected *** evaluate the pathogenicity of the isolates in vivo artificial infection challenge test was conducted through immersion *** in vivo pathogenicity test was done at three different temperatures(18◦C,28◦C,and 37◦C)to examine the effects of temperature on the pathogenicity of the *** commercial antibiotics were used to find out the antibiogram profile of the ***,susceptibility of the isolates was screened against 19 medicinal plants extracts.A total of 38 out of 46 isolates isolated from Vietnam climbing perch(***)suffering from EUS were phenotypically identified as Aeromonas *** randomly selected isolates were further confirmed as *** by analyzing the 16S rRNA gene sequence *** pathogenicity of the isolates was found moderate to high(41%-80%mortality)in the artificially challenged *** highest and lowest mortality was obtained in the fish challenged with the isolates BFKA33 and BFKA12,*** isolates were found resis

关键词： Aeromonas veronii Anabas testudineus,Epizootic ulcerative syndrome Antibiogram Medicinal plant extracts

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Genome-wide identification,characterization and functional prediction of the SRS gene family in sesame(Sesamum indicum L.)

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Oil Crop Science 2024年第2期9卷 69-80页

作者： Farjana Afroz Susmi tasmina Islam Simi Md Nahid Hasan Md Abdur Rahim Department of Genetics and Plant Breeding Sher-e-Bangla Agricultural UniversityDhaka1207Bangladesh

Sesame(Sesamum indicum L.)is an ancient oilseed crop of the Pedaliaceae family with high oil content and potential health *** RELATED SEQUENCE(SRS)proteins are the transcription factors(TFs)specific to plants that contain RING-like zinc finger domain and are associated with the regulation of several physiological and biochemical *** also play vital roles in plant growth and development such as root formation,leaf development,floral development,hormone biosynthesis,signal transduction,and biotic and abiotic stress ***,the SRS gene family was not reported in sesame *** this study,identification,molecular characterization,phylogenetic relationship,cis-acting regulatory elements,protein-protein interaction,syntenic relationship,duplication events and expression pattern of SRS genes were analyzed in *** identified total six SiSRS genes on seven different linkage groups in the *** genome by comparing with the other species,including the model plant Arabidopsis *** SiSRS genes showed variation in their structure like2–5 exons and 1–4 *** other species,SiSRS proteins also contained‘RING-like zinc finger'and‘LRP1'***,the SiSRS genes were clustered into subclasses via phylogenetic analysis with proteins of ***,***,and some other plant *** cis-acting regulatory elements analysis revealed that the promoter region of SiSRS4(SIN_1011561)showed the highest 13 and 16 elements for light-and phytohormone-responses whereas,SiSRS1(SIN_1015187)showed the highest 15 elements for *** ABREs,or ABA-responsive elements,were found in a maximum of 8 copies in the SiSRS3(SIN 1009100).Moreover,the available RNA-seq based expression of SiSRS genes revealed variation in expression patterns between stress-treated and non-treated samples,especially in drought and salinity conditions *** SiSRS genes like SiSRS1(SIN_1015187)and SiSRS5(SIN_1021065),also exhibited variabl

关键词： SiSRS gene family SHI Transcription factor Characterization Sesamum indicum

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Analysis of Immune Responses and Serological Cross Reactivities among Vibrio cholerae O1,Shigella flexneri 2a and Haemophilus influenzae b

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Cellular & Molecular Immunology 2008年第5期5卷 393-396页

作者： Fazle Rabbi Nasreen Sultana tasmina Rahman H. M. Al-Emran M. Nizam Uddin Mahbub Hossain Kazi Selim Anwar Mahmuda Yasmin Jamalun Nessa Chowdhury R. Ahsan Department of Microbiology University of Dhaka Department of Pharmacy University of Asia Pacific Institute of Public Health Mohakhali

Antigenic determinants expressed on the bacterial cell surface are of importance in the serological characterization and microbiological diagnosis. The bacterial strains carrying these identical or similar antigenic epitopes might react with antibodies produced against other strains. In this study, strong immunogenicity and antigenic cross reactivity were demonstrated among V. cholerae O1, S. flexnerii 2a and H. influenzae b surface components. The enzyme linked immunosorbent assay （ELISA） results were supported by Western blot analysis, where at least 20 antigenic bands, were obtained in each of the reactions, when the surface components were reacted with the homologous antisera. The indirect ELISA results also demonstrated high degree of antigenic relatedness between the surface components of these species, where each surface component was reacted with the heterologous antisera. Western blot analysis also revealed cross reactions between the surface components suggesting common distribution of antigens/epitopes in these bacterial species. This study, thus, gave a clear idea of the level of antigenic sharing and variations among the pathogenic V. cholerae O1, S. flexneri 2a and H. influenzae b strains, which in future, may help in selecting a proper candidate for vaccines and immunodiagnostics development.

关键词： V. cholerae O1 S. flexnerii 2a H. influenzae b cross antigen

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In vitro α-amylase inhibitory activity and in vivo hypoglycemic effect of ethyl acetate extract of Mallotus repandus (Willd.) Muell. stem in rat model

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Journal of Coastal Life Medicine 2014年第9期 721-726页

作者： Md.Rakib Hasan Nizam Uddin Md.Monir Hossain Md.Mahadi Hasan Md.Emtiaz Yousuf Swagata Sarker Lopa tasmina Rahman Mohammad Shahabuddin Kabir Choudhuri Department of Pharmacy Jahangirnagar UniversitySavarDhaka-1342Bangladesh

Objective:To investigate the therapeutic effects of ethyl acetate extract of Mallotus repandus stem in α-amylase inhibitory activity(in vitro)and hypoglycemic activity in normal and glucose induced hyperglycemic rats(in vivo).Methods:Ethyl acetate extract of Mallotus repandus stem was tested for the presence of phytochemical constituents,α-amylase inhibitory activity and hypoglycemic effect in normal rats and glucose induced hyperglycemic ***:Presence of different types of phytochemicals was identified in the *** extract has moderate α-amylase inhibitory activity[IC_(50)=(2.038±0.033)mg/mL]as compared to *** does 1000 mg/kg significantly reduced(P<0.0100)fasting blood glucose level in normal *** oral glucose tolerance test,both 1000 and 2000 mg/kg doses showed good hypoglycemic activity(P<0.0001)like glibenclamide in each specific hour after *** time effect in oral glucose tolerance test was found extremely significant(P<0.0001)with F(3,48)value=***:These findings suggest that this plant may be a potential source for the development of new oral hypoglycemic agent.

关键词： Mallotus repandus α-Amylase Diabetes mellitus Hypoglycemic Oral glucose tolerance test

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