Aeromonas veronii isolated from climbing perch(Anabas testudineus)suffering from epizootic ulcerative syndrome(EUS)
作者机构:Institute of Biotechnology and Genetic EngineeringBangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur1706Bangladesh Department of Fisheries ManagementFaculty of FisheriesBangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur1706Bangladesh
出 版 物:《Aquaculture and Fisheries》 (渔业学报(英文))
年 卷 期:2023年第8卷第3期
页 面:288-295页
核心收录:
主 题:Aeromonas veronii Anabas testudineus,Epizootic ulcerative syndrome Antibiogram Medicinal plant extracts
摘 要:Motile Aeromonads are responsible for various diseases in fishes that cause significant economic losses in *** *** also frequently isolated from fish suffering from Epizootic Ulcerative Syndrome(EUS).The present study was conducted to isolate and identify *** from Vietnam climbing perch(Anabas testudineus)suffering from EUS,evaluate the pathogenicity of the isolates,and find out antibiogram profile and susceptibility of the isolates to medicinal plant *** climbing perch(***)suffering from EUS was collected from fish farms located at Savar,Dhaka and bacteria were isolated from the external ulcerative lesion,and kidney and liver of the infected fish on nutrient agar *** phenotypic identification of the bacterial isolates was performed following morphological,physiological and biochemical characterization *** 16S rRNA gene sequencing was accomplished for further molecular identification of five selected *** evaluate the pathogenicity of the isolates in vivo artificial infection challenge test was conducted through immersion *** in vivo pathogenicity test was done at three different temperatures(18◦C,28◦C,and 37◦C)to examine the effects of temperature on the pathogenicity of the *** commercial antibiotics were used to find out the antibiogram profile of the ***,susceptibility of the isolates was screened against 19 medicinal plants extracts.A total of 38 out of 46 isolates isolated from Vietnam climbing perch(***)suffering from EUS were phenotypically identified as Aeromonas *** randomly selected isolates were further confirmed as *** by analyzing the 16S rRNA gene sequence *** pathogenicity of the isolates was found moderate to high(41%-80%mortality)in the artificially challenged *** highest and lowest mortality was obtained in the fish challenged with the isolates BFKA33 and BFKA12,*** isolates were found resis