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Asian Agricultural Research 2019年第5期11卷 46-49页

作者： Meipeng LE Yulian WU Yuting XIAO taojian he College of Urban Construction Jiangxi Normal University

With the rapid development of the city,the old city has gradually lost its former glory,and the old community accounts for a large proportion in the city,and is located in the core area of the city. At present,there are some problems in the old community,such as high degree of aging,poor quality of living space,and lack of outdoor space. The old community transformation is mainly in the way of ' guided reconstruction',it has completely destroyed the original historical environment and the spirit of the place. Therefore,taking Xingchaibeiyuan Community in Nanchang City as an example,we deeply explore the strategy of micro-renewal of community outdoor space,so as to provide a reference for the renewal of outdoor space in this kind of old residential area.

关键词： Communities for all ages Outdoor space Micro-renewal The elderly Old urban areas

Layout Modes and Characteristics of Site Selection of Traditional Ancestral Halls in Jiangxi: A Case Study of Yu Ancestral Hall in Wangkou Village, Wuyuan County

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Journal of Landscape Research 2019年第5期11卷 119-122页

作者： ZHOU Bo he taojian LENG Haoran ZheNG Wangyi College of City Construction Jiangxi Normal University

Traditional ancestral halls play an important role in the inheritance of the clan *** carry strong clan ritual culture and are the hall of the clan *** China’s traditional society,the clan concept was very deep,and a village was often formed by a single *** ancestral hall is a place where people sacrifice their ancestors and thus is given the most important significance,which is related to the rise and fall of the *** on the instinct of biological benefit and avoidance,the site selection of ancestral halls must be the most *** this paper,the site selection of Yu Ancestral Hall in Wangkou Village,Wuyuan County,Jiangxi Province was surveyed and analyzed,and the layout modes and artistic characteristics of site selection of traditional ancestral halls in Jiangxi were studied to provide reference for the protection and sustainable development of traditional ancestral halls.

关键词： Wangkou Village Yu Ancestral Hall Traditional ancestral halls Site selection

A Brief Analysis of Successful Experience of French Classical Gardens for the Construction of Chinese Gardens

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Asian Agricultural Research 2019年第11期11卷 28-30页

作者： Guilin CheNG Yuefang YU taojian he Chuyun LIN Lan LUO College of City Construction Jiangxi Normal UniversityNanchang 330022China

This paper reviews the course of the development of the French garden system,interprets the French gardening techniques and characteristics,and analyzes its features,as well as the classical garden works at that *** the analysis of the concept of French garden construction,the prospect of Chinese garden construction is finally foreseen.

关键词： Landscape architecture France Garden Construction concept

Exploration and Innovation of Village Planning Compilation Based on the Background of Land Space Planning

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Asian Agricultural Research 2019年第11期11卷 15-17页

作者： Chen BAO Yuefang YU Chuyun LIN taojian he Lan LUO College of City Construction Jiangxi Normal UniversityNanchang 330022China

Compilation work of land space planning at village level is important point in promoting the strategy of rural revitalization and the key for specific implementation of land space *** on analyzing the existing problems in the planning at village level in China,overall guidelines and specific measures of land space planning compilation at village level are proposed,and some thoughts and suggestions on how to perfect its legal status,considering regional characteristics and valuing landscape design are put forward.

关键词： Village Planning Land space planning Rural revitalization

CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant

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Nano-Micro Letters 2022年第10期14卷 83-94页

作者： Chenshuo Wu Zhi Chen Chaozhou Li Yabin Hao Yuxuan Tang Yuxuan Yuan Luxiao Chai taojian Fan Jiangtian Yu Xiaopeng Ma Omar AAl-Hartomy SWageh Abdullah GAl-Sehemi Zhiguang Luo Yaqing he Jingfeng Li Zhongjian Xie Han Zhang International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of EducationInstitute of Microscale OptoelectronicsCollege of Physics and Optoelectronic EngineeringShenzhen UniversityShenzhen 518060People’s Republic of China Shenzhen International Institute for Biomedical Research Shenzhen 518116People’s Republic of China Department of Respiratory Shenzhen Children’s HospitalShenzhen 518038People’s Republic of China Institute of Pediatrics Shenzhen Children’s HospitalShenzhen 518038People’s Republic of China Shenzhen Han’s Tech Limited Company Shenzhen 518000People’s Republic of China Hospital of Guangzhou Medical University Qingyuan city People’s HospitalQingyuan 511518People’s Republic of China Department of Physics Faculty of ScienceKing Abdulaziz UniversityJeddah 21589Saudi Arabia Research Center for Advanced Materials Science(RCAMS) King Khalid UniversityP.O.Box 9004Abha 61413Saudi Arabia Department of Chemistry College of ScienceKing Khalid UniversityP.O.Box 9004Abha 61413Saudi Arabia Zhongmin(Shenzhen)Intelligent Ecology Co. LtdShenzhen 518055People’s Republic of China Shenzhen Center for Disease Control and Prevention Shenzhen 518055People’s Republic of China

Coronavirus disease 2019(COVID-19)is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The gold standard method for the diagnosis of SARS-CoV-2 depends on quantitative reverse transcription-polymerase chain reaction till now,which is time-consuming and requires expensive instrumentation,and the confirmation of variants relies on further sequencing ***,we first proposed a robust technique-methodology of electrochemical CRISPR sensing with the advantages of rapid,highly sensitivity and specificity for the detection of SARS-CoV-2 *** enhance the sensing capability,gold electrodes are uniformly decorated with electro-deposited gold *** DNA template identical to SARS-CoV-2 Delta spike gene sequence as model,our biosensor exhibits excellent analytical detection limit(50 fM)and high linearity(R2=0.987)over six orders of magnitude dynamic range from 100 fM to 10 nM without any nucleic-acid-amplification *** detection can be completed within 1 h with high stability and specificity which benefits from the CRISPR-Cas ***,based on the wireless micro-electrochemical platform,the proposed biosensor reveals promising application ability in point-of-care testing.

关键词： SARS-COV-2 variant Methodology of electrochemical CRISPR sensing(MOECS) Gold nanoparticles(AuNPs) Point-of-care testing(POCT)

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A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2

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National Science Review 2022年第8期9卷 100-109页

作者： Zhi Chen Jingfeng Li Tianzhong Li taojian Fan Changle Meng Chaozhou Li Jianlong Kang Luxiao Chai Yabin Hao Yuxuan Tang Omar A.Al-Hartomy Swelm Wageh Abdullah G.Al-Sehemi Zhiguang Luo Jiangtian Yu Yonghong Shao Defa Li Shuai Feng William J.Liu Yaqing he Xiaopeng Ma Zhongjian Xie Han Zhang Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education Shenzhen Institute of Translational Medicine Department of Otolaryngology Shenzhen Second People’s Hospital the First Affiliated Hospital Institute of Microscale Optoelectronics Shenzhen University Shenzhen International Institute for Biomedical Research Shenzhen Han’s Tech Limited Company Shenzhen Metasensing Tech Limited Company Department of Physics Faculty of Science King Abdulaziz University Research Center for Advanced Materials Science (RCAMS) King Khalid University Department of Chemistry College of Science King Khalid University Zhongmin (Shenzhen) Intelligent Ecology Co.Ltd Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Department of Laboratory Medicine Shenzhen Children’s Hospital Optoelectronics Research Center School of Science Minzu University of China NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Research Unit of Adaptive Evolution and Control of Emerging Viruses Chinese Academy of Medical Sciences Institute of Pathogenic Organism Shenzhen Center for Disease Control and Prevention Respiratory Department Shenzhen Children’s Hospital Institute of Pediatrics Shenzhen Children’s Hospital

The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus, and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed. On the other hand, several concerning SARS-Co V-2 variants,including Omicron, are required to be identified as soon as the samples are identified as ‘positive'.Unfortunately, a traditional PCR test does not allow their specific identification. herein, for the first time,we have developed MOPCS(Methodologies of Photonic CRISPR Sensing), which combines an optical sensing technology-surface plasmon resonance(SPR) with the ‘gene scissors' clustered regularly interspaced short palindromic repeat(CRISPR) technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants. MOPCS is a low-cost, CRISPR/Cas12a-systemempowered SPR gene-detecting platform that can analyze viral RNA, without the need for amplification,within 38 min from sample input to results output, and achieve a limit of detection of 15 fM. MOPCS achieves a highly sensitive analysis of SARS-Co V-2, and mutations appear in variants B.1.617.2(Delta),B.1.1.529(Omicron) and BA.1(a subtype of Omicron). This platform was also used to analyze some recently collected patient samples from a local outbreak in China, identified by the Centers for Disease Control and Prevention. This innovative CRISPR-empowered SPR platform will further contribute to the fast, sensitive and accurate detection of target nucleic acid sequences with single-base mutations.

关键词： surface plasmon resonance CRISPR SARS-CoV-2 Omicron variant

湖南桃江稻瘟病菌遗传多样性分析及无毒基因的鉴定

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西南农业学报 2019年第9期32卷 2040-2045页

作者：贺雄丁朝辉周瑚朱华珺李俊俊丁宇倩胡立冬任佐华刘二明湖南农业大学植物保护学院湖南长沙410128 植物病虫害生物学与防控湖南省重点实验室湖南长沙410128 南方粮油作物协同创新中心湖南长沙410128 湖南省桃江县农业局植保植检站湖南桃江413400

【目的】为明确湖南桃江地区稻瘟病菌遗传多样性和无毒基因的组成及变化趋势。【方法】利用8对SSR引物对2017年从湖南桃江病圃多个晚稻品种上分离纯化的29个稻瘟病单孢菌株进行遗传多样性分析,并利用18个已知抗瘟基因的水稻近等基因系(N... 详细信息

【目的】为明确湖南桃江地区稻瘟病菌遗传多样性和无毒基因的组成及变化趋势。【方法】利用8对SSR引物对2017年从湖南桃江病圃多个晚稻品种上分离纯化的29个稻瘟病单孢菌株进行遗传多样性分析,并利用18个已知抗瘟基因的水稻近等基因系(NILs)对其中的19个特异性稻瘟病菌株进行无毒基因鉴定。【结果】在相似系数0.70,差异系数0.30水平下,29个供试菌株可划分为7个宗谱,优势宗谱Ⅰ有12个菌株,占参测菌数的41.3%,宗谱Ⅴ有7个菌株,占总菌数的24.1%,其余5个宗谱分别有1?3个菌株,占总菌数的34.6%;通过无毒基因鉴定18个菌株致病频率(致病频率=感病水稻数/所有测试水稻品种数XlOO%)在35%?75%。强致病力菌株(致病频率≥70%)3株,占总菌数的15.7%;较强致病力菌株(70%)致病频率≥50%)7株,占总菌数36.8%;中等致病力菌株(50%>致病频率3=20%)9株,占总菌数的47.5%;供试菌株平均致病频率为49.86%。19个菌株对NILs水稻品系毒力频率(毒力频率=对测试水稻品系有毒力的菌株数/所有菌株数XlOO%)超过70%的抗性基因为Pi-k^s、Pi-z^t、Pi-ta,属强毒力菌株;毒力频率介于50%?70%的抗性基因为Pi-k^p、Pi-z^5、Pi-t、Pi-sh、Pi-5,,属于中等毒力菌株;对其余抗性基因的毒力频率低于50%。【结论】湖南桃江稻瘟病菌群体具有丰富的遗传多样性,而且存在复杂的致病性分化;抗性基因Pi-3、Pi-i、Pi-11、Pi-19、Pi-12对桃江地区稻瘟病菌抗性优良,可在抗病育种中加以利用。

关键词：稻瘟病菌 SSR分子标记遗传多样性无毒基因

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振兴桃江茶业的思考

茶叶通讯 2008年第2期35卷 37-39,41页

作者：高大可卢夏英何世安丁艳云熊艳辉湖南省桃江县农业局桃江413400 湖南省桃江县牛田镇农技站桃江413400

文章总结了桃江茶业的历史和现状,分析了桃江茶业的优势和问题,提出了振兴桃江茶业的思路、目标和对策。

关键词：桃江茶业优势问题思路对策

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