检索结果-南通市图书馆

α-Mangostin and apigenin induced the necrotic death of BT474 breast cancer cells with autophagy and inflammation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2018年第11期8卷 519-526页

作者： Teeranai Ittiudomrak Songchan Puthong tanapat palaga Sittiruk Roytrakul Chanpen Chanchao Program in Biotechnology Faculty of Science Chulalongkorn University Institute of Biotechnology and Genetic Engineering Chulalongkorn University Department of Microbiology Faculty of Science Chulalongkorn University National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency Department of Biology Faculty of Science Chulalongkorn University

Objective: To find new compounds in order to overcome the mainstay of metastatic breast cancer due to the adverse side effects from, and increasing resistance to, current chemotherapeutic agents. Methods: 毩-Mangostin and apigenin were reported in comparison to doxorubicin, a chemotherapeutic drug. Ductal carcinoma(BT474) cell line and nontumorigenic epithelial tissue from mammary gland(MCF-10 A) were used. Cell viability assessment was calculated by the standard 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Cell morphology was investigated by light microscopy. By flow cytometry analysis, programmed cell death was observed using annexin observed using propidium iodide st桋 and propidium iodide staining while cell-cycle arrest wasaining. Change in transcriptional expression was evaluated by real-time quantitative reverse transcription PCR. Results: In 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, the result revealed and apigenin were more cytotoxic to BT474 cells. Longer exposure times to 毩-mangostin enin caused more floating cells and a lower density of adhered cells wi毩-mangostin and apigth more vacuoles present in the colonies in BT474 only. 毩-Mangostin and apigenin caused necrosis in BT474 cells in a 24 h exposure, but a small amount of early apoptotic cells could also be detected at 24, 48 and 72 h exposure, whereas doxorubicin caused early apoptosis to BT474 cells at 24 h. Transcript expression and activity analysis supported caspase-3 was involved in the death of BT474 cells treated by all compounds. Moreover, 毩-mangostin and apigenin arrested the cellcycle at the G1-phase, but at the G2/M-phase by doxorubicin. All three compounds induced a change in transcript expression levels of inflammation-associated, proto-oncogene, autophagyassociated and apoptosis-associated genes. Conclusions: ntial new sources of chemotherapeuti毩-Mangostin and apigenin are worth investigating as potec agents for breast cancer treatment.

关键词： α-Mangostin Apigenin Breast cancer Cell cycle arrest Necrosis

Direct regulation of interleukin-6 expression by Notch signaling in macrophages

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2012年第2期9卷 155-162页

作者： Wipawee Wongchana tanapat palaga Graduate Program in Biotechnology Faculty of Science Chulalongkorn University Bangkok Thailand Department of Microbiology Faculty of Science Chulalongkorn University Bangkok Thailand

Interleukin-6 （IL-6） is a pleiotropic, pro-inflammatory cytokine produced by various types of cells, including macrophages. Within the IL-6 gene promoter region, the signature binding motif of CBFI/Su（H）/Lag-1 （CSL）, a key DNA-binding protein in the Notch signaling pathway, was identified and found to overlap with a consensus nuclear factor （NF）-κB-binding site. Notch signaling is highly conserved and is involved in the regulation of biological functions in immune cells. In this study, we investigated the role of Notch signaling in the regulation of the IL-6transcript in murine macrophages. The upregulation of Notch1 protein levels and the appearance of cleaved Notch1 （Va11744） correlated well with the increased IL-6 mRNA expression levels in murine primary bone marrow-derived macrophages （BMMφ） after activation by lipopolysaccharide （LPS） together with interferon-gamma （IFN-γ）. Treatment of BMMφ with the γ-secretase inhibitor IL-CHO to suppress the transduction of Notch signaling resulted in a partial decrease in the level of IL-6mRNA and the amount of I L-6 protein produced. In contrast, the overexpression of a constitutively activated intracellular Notch I protein （N^IC） in the RAW264.7 macrophage-like cell line resulted in significantly higher IL-6transcript expression levels than in cells transfected with the empty vector control. The NF-κB inhibitor completely abrogated IL-6 mRNA expression induced by the overexpression of N^IC. Chromatin immunoprecipitation （CHIP） using an anti-Notch1 antibody demonstrated that Notch 1 is associated with the IL-6promoter in RAW264.7 cells activated by LPS/IFN-γ but not in unstimulated cells. Taken together, these results strongly suggest that Notch 1 positively regulates IL-6 expression via NF-κB in activated macrophages.

关键词： CSL interleukin-6 macrophages NF-κB Notch signaling

Increased ATG5-ATG12 in hepatitis B virus-associated hepatocellular carcinoma and their role in apoptosis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2016年第37期22卷 8361-8374页

作者： Areerat Kunanopparat Ingorn Kimkong tanapat palaga Pisit Tangkijvanich Boonchoo Sirichindakul Nattiya Hirankarn Center of Excellence in Immunology and Immune-mediated Diseases Department of Microbiology Faculty of Medicine Chulalongkorn University Department of Microbiology Faculty of Science Kasetsart University Center for Advanced Studies in Tropical Natural Resources National Research University - Kasetsart University Department of Microbiology Faculty of Science Chulalongkorn University Research Unit of Hepatitis and Liver Cancer Department of Biochemistry Faculty of Medicine Chulalongkorn University Department of Surgery Faculty of Medicine Chulalongkorn University

AIM To investigate autophagy-related genes, particularly ATG12, in apoptosis and cell cycle in hepatitis B virus(HBV)-associated hepatocellular carcinoma(HCC) and non-HBV-HCC cell *** The expression of autophagy-related genes in HBVassociated hepatocellular carcinoma and non-HBV-HCC cell lines and human liver tissues was examined by quantitative real-time reverse transcriptase-polymerase chain reaction(q RT-PCR) and western blotting. The silencing of target genes was used to examine the function of various genes in apoptosis and cell cycle progression. RESULTS The expression of autophagy related genes ATG5, ATG12, ATG9 A and ATG4 B expression was analyzed in Hep G2.2.15 cells and compared with Hep G2 and THLE cells. We found that ATG5 and ATG12 m RNA expression was significantly increased in Hep G2.2.15 cells compared to HepG 2 cells(P < 0.005). Moreover, ATG5-ATG12 protein levels were increased in tumor liver tissues compared to adjacent non-tumor tissues mainly from HCC patients with HBV infection. We also analyzed the function of ATG12 in cell apoptosis and cell cycle progression. The percentage of apoptotic cells increased by 11.4% in ATG12-silenced Hep G2.2.15 cells(P < 0.005) but did not change in ATG12-silenced HepG 2 cells under starvation with Earle's balanced salt solution. However, the combination blockade of Notch signaling and ATG12 decreased the apoptotic rate of HepG 2.2.15 cells from 55.6% to 50.4%(P < 0.05). CONCLUSION ATG12 is important for HBV-associated apoptosis and a potential drug target for HBV-HCC. Combination inhibition of ATG12/Notch signaling had no additional effect on HepG 2.2.15 apoptosis.

关键词： Autophagy Hepatitis B virus Hepatocellular carcinoma ATG12 Apoptosis Cell cycle Notch signaling

Delta-like ligand 4 in hepatocellular carcinoma intrinsically promotes tumour growth and suppresses hepatitis B virus replication

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2018年第34期24卷 3861-3870页

作者： Areerat Kunanopparat Jiraphorn Issara-Amphorn Asada Leelahavanichkul Anapat Sanpavat Suthiluk Patumraj Pisit Tangkijvanich tanapat palaga Nattiya Hirankarn Center of Excellence in Immunology and Immune Mediated Diseases Department of Microbiology Faculty of Medicine Chulalongkorn University Department of Pathology Faculty of Medicine Chulalongkorn University Center of Excellence for Microcirculation Department of Physiology Faculty of Medicine Chulalongkorn University Research Unit of Hepatitis and Liver Cancer Department of Biochemistry Faculty of Medicine Chulalongkorn University Department of Microbiology Faculty of Science Chulalongkorn University

AIM To investigate the role of Delta-like ligand 4(DLL4) on tumour growth in hepatitis B virus(HBV)-associated hepatocellular carcinoma(HCC) in *** We suppressed DLL4 expression in an HBV expressing HCC cell line, HepG2.2.15 and analysed the growth ability of cells as subcutaneous tumours in nude mice. The expression of tumour angiogenesis regulators, VEGF-A and VEGF-R2 in tumour xenografts were examined by western blotting. The tumour proliferation and neovasculature were examined by immunohistochemistry. The viral replication and viral protein expression were measured by quantitative PCR and western blotting, *** Eighteen days after implantation, tumour volume in mice implanted with sh DLL4 HepG2.2.15 was significantly smaller than in mice implanted with control HepG2.2.15(P < 0.0001). The levels of angiogenesis regulators, VEGF-A and VEGF-R2 were significantly decreased in implanted tumours with suppressed DLL4 compared with the control group(P < 0.001 and P < 0.05, respectively). Furthermore, the suppression of DLL4 expression in tumour cells reduced cell proliferation and the formation of new blood vessels in tumours. Unexpectedly, increased viral replication was observed after suppression of DLL4 in the *** This study demonstrates that DLL4 is important in regulating the tumour growth of HBV-associated HCC as well as the neovascularization and suppression of HBV replication.

关键词： hepatocellular carcinoma Notch signalling Delta-like ligand 4 HepG2.2.15

Production of a monoclonal antibody against oxytetracycline and its application for oxytetracycline residue detection in shrimp

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2014年第2期15卷 165-172页

作者： Tossapon WONGTANGPRASERT Wirongrong NATAKUATHUNG Umaporn PIMPITAK Anumart BUAKEAW tanapat palaga Kittinan KOMOLPIS Nanthika KHONGCHAREONPORN Program in Biotechnology Faculty of Science Chulalongkorn University Institute of Biotechnology and Genetic Engineering Chulalongkorn University Department of Microbiology Faculty of Science Chulalongkorn University

A novel monoclonal antibody （MAb） against oxytetracycline （OTC） was generated and characterized. The MAb was used in the development of an enzyme-linked immunosorbant assay （ELISA）-based detection system. An OTC-bovine serum albumin （BSA） conjugate was prepared and used in the immunization of mice. A conventional somatic cell fusion technique was used to generate MAb-secreting hybridomas denoted 2-4F, 7-3G, and 11-11A. An indirect competitive ELISA （icELISA） was applied to measure the sensitivity and specificity of each MAb in terms of its 50% inhibitory concentration （IC50） and percentage of cross-reactivity, respectively. MAb 2-4F exhibited the highest sensitivity, with an ICs0 of 7.01 ng/ml. This MAb showed strong cross-reactivity to rolitetracycline, but no cross- reactivity to other unrelated antibiotics. When MAb 2-4F was used to detect OTC from shrimp samples, the recoveries were in the range of 82%-118% for an intra-assay and 96%-113% for an inter-assay. The coefficients of variation of the assays were 3.9%-13.9% and 5.5%-14.9%, respectively.

关键词： Oxytetracycline Monoclonal antibody Enzyme-linked immunosorbant assay (ELISA) Shrimp