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Identification of genes involved in regulating MnSOD2 production and root colonization in Bacillus cereus 905

Journal of Integrative Agriculture 2021年第6期20卷 1570-1584页

作者： GAO tan-tan DING Ming-zheng LI Yan ZENG Qing-chao WANG Qi Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs Beijing University of AgricultureBeijing 102206P.R.China Department of Plant Pathology College of Plant ProtectionChina Agricultural UniversityBeijing 100193P.R.China

sodA2-encoding manganese-containing superoxide dismutase(MnSOD2)in Bacillus cereus 905 plays an essential role in antioxidative stress,nutrition utilization,rhizosphere and phyllosphere ***,the genes involved in regulating the sod A2 expression have not been clearly elucidated in *** this study,a genome-wide random insertion mutagenesis was constructed by using transposon TnYLB-1 to identify novel genes regulating the sodA2 *** mutants that changed the sodA2 expression at both m RNA and protein levels were finally *** analysis and BLAST data showed that the genes disrupted by TnYLB-1 in *** 905 shared high conservations with those in the *** type strain,ATCC *** genes encode heat-inducible transcription repressor,chloride channel protein,recombinase A,ferrous iron transport protein,nucleoside diphosphate kinase,and histidine ***,we also provided the evidence that the genes regulating the sodA2 expression could influence colonization ability of *** 905 on wheat ***,those genes downregulating the sodA2 expression significantly reduced bacterial colonization on wheat roots,and mutants with increased MnSOD2 activities could enhance bacterial population densities on wheat roots to a certain *** work provided information that multiple genes are involved in MnSOD2 production and wheat root *** molecular regulatory pathways through which these genes modulate the sod A2 expression and root colonization need to be investigated extensively in the future.

关键词： Bacillus cereus sod A2 TnYLB-1 transposon colonization

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Nickel-iron borate coated nickel-iron boride hybrid for highly stable and active oxygen evolution electrocatalysis

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Chinese Chemical Letters 2020年第9期31卷 2469-2472页

作者： Pengyu Han tan tan Fei Wu Ping Cai Gongzhen Cheng Wei Luo College of Chemistry and Molecular Sciences Wuhan UniversityWuhan 430072China Department of Orthopaedic Surgery Renmin Hospital of Wuhan UniversityWuhan 430060China Suzhou Institute of Wuhan University Suzhou 215123China

The development of efficient and cost-effective electrocatalysts toward anodic oxygen evolution reaction(OER) is crucial for the commercial application of electrochemical water *** the most promising electrocatalysts,the OER performances of nickel-iron-based materials can be further improved by introducing metalloid elements to modify their electron ***,we developed an efficient hybrid electrocatalyst with nickel-iron boride(NiFeB) as core and amorphous nickel-iron borate(NiFeBi)as shell(NiFeB@NiFeBi) via a simple aqueous *** obtained NiFeB@NiFeBi exhibits a small overpotential of 237 mV at 10 mA/cm^2 and Tafel slope of 57.65 mV/dec in 1.0 mol/L KOH,outperforming most of the documented precious-metal-free based *** from the in situ formed amorphous NiFeBi layer,it shows excellent stability toward the oxygen evolution reaction(OER).These findings might provide a new way to design advanced precious-metal-free electrocatalysts for OER and the application of electrochemical water splitting.

关键词： Oxygen evolution reaction Metal boride Metal borate Core-shell

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Renal artery embolization in the treatment of urinary fistula after renal duplication:A case report and review of literature

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World Journal of Clinical Cases 2021年第13期9卷 3177-3184页

作者： Tao Yang Jun Wen tan-tan Xu Wen-Jing Cui Jian Xu Department of Interventional Radiology Jinling HospitalMedical School of Nanjing UniversityNanjing 210029Jiangsu ProvinceChina Graduate School Xuzhou Medical UniversityXuzhou 221000Jiangsu ProvinceChina Department of Radiology The Affiliated Hospital of Nanjing University of Chinese MedicineNanjing 210029Jiangsu ProvinceChina

BACKGROUND Duplicate renal malformation is a congenital disease of the urinary system,with an incidence rate of 0.8%.Surgical treatment is suitable for symptomatic *** fistula is one of the complications of ***-term urinary fistula has a great impact on patients'*** SUMMARY This article mainly reports on a 47-year-old man with duplication of kidney deformity,long urinary fistula after partial nephrectomy,and no improvement after conservative *** have achieved positive results in the arterial embolization treatment of the residual renal artery,indicating that selective arterial embolization is a good way to treat urinary fistula after partial *** is worth noting that this patient violated the Weigert-Meyer law,which also gave us more *** Renal artery embolization may be a simple and safe method to treat urinary fistula inefficacy with conservative treatment.

关键词： Renal artery embolization Urinary leakage Urinary fistula Duplicate renal malformation Selective arterial embolization Case report

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Transcriptional profile of gene clusters involved in the methylerythritol phosphate pathway in Bacillus subtilis 916

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Journal of Integrative Agriculture 2019年第3期18卷 644-655页

作者： XIAO Ya-jing GAO tan-tan PENG Qi ZHANG Jie SUN Dong-mei SONG Fu-ping College of Life Science and Technology Heilongjiang Bayi Agricultural University State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences

The methylerythritol phosphate pathway is responsible for the biosynthesis of terpenoids, the largest class of secondary metabolites. Although the structures and functions of the proteins involved in this pathway have been well studied in Bacillus subtilis, only a few studies have reported the transcriptional profile of the genes involved. Therefore, we analyzed methylerythritol phosphate pathway genes in the genome of B. subtilis 916, which has been developed as a biological control agent against some rice diseases in China. Our results showed that methylerythritol phosphate pathway genes were distributed throughout the genome of this strain. These genes were transcribed during both the exponential and stationary phases. We further confirmed the transcription units of dxs, dxr, ispD, ispF, ipK, ispG, ispH, idi, and ispA in B. subtilis 916 through reverse transcription-PCR analyses; the results showed that these nine genes were located in seven different operons. The transcript start sites of the seven different operons were determined by 5′-rapid amplification of cDNA ends-PCR. Thus, our study provides a molecular basis at the transcriptional level for investigating homoterpene synthesis in the methylerythritol phosphate pathway of B. subtilis 916.

关键词： methylerythritol phosphate pathway transcription Bacillus subtilis biological control

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Strength and failure characteristics of sandstone containing two circular holes filled with two types of inclusions under uniaxial compression

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Journal of Central South University 2017年第11期24卷 2487-2495页

作者： DU Ming-rui JING Hong-wen SU Hai-jian ZHU tan-tan CHEN Min-liang State Key Laboratory for Geomehanics&Deep Underground Engineering(China University of Mining and Technology) Xuzhou 221116China

Plate shaped sandstones containing two fabricated circular holes that were filled with gypsum and high-strength concrete respectively were prepared for studying the effects of ligament length L ligament incline angle α, as well as filling modes on their strength properties and failure modes. The results show that the initial cracks can be categorized as wing crack, axial tensile crack and curved tensile crack. The failure modes of ligaments can be categorized as mode of single inclined crack, mode of single axial crack and mode of two parallel cracks. The final failure modes of all specimens can be categorized as the tension-shear mixed failure and shear failure. The strength of inclusions shows little influence on the final failure modes of specimens, while the failure modes vary with L and α. When α is a fixed value, the peak strength σc and elastic modulus Ec of tested specimens increase firstly with increasing L and reaches to the maximum value at L of 16 mm, then declines. When L is a fixed value, σc declines firstly and then turns to increase as α increases to 75° from 45°, while Ec increases linearly. The axial stress σp performs the similar variation trends with those of σc versus increasing L and α when ligaments fail.

关键词： sandstone two circular holes inclusions uniaxial compression failure modes strength properties

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Fluorometric enzyme immunosensing system based on natural product resveratrol for horseradish peroxidase and Ag/SiO_2 nanoparticles

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Science China Chemistry 2008年第3期51卷 233-240页

作者： GONG FuChun, CAO Zhong, tan ShuZhen, tan YaFei & HE XiaoChun College of Chemistry and Environmental Engineering, Changsha University of Science and Technology, Changsha 410076, China College of Chemistry and Environmental Engineering Changsha University of Science and Technology Changsha China

The properties of resveratrol (3′, 4′, 5-trihydroxystlbene, RST) were for the first time evaluated as a potential substrate for horseradish peroxidase (HRP)-catalyzed fluorogenic reaction. The properties of RST for use as fluorogenic substrates for HRP and its application in immunoassays were compared with commercially available substrates such as p-hydroxyphenylpropionic acid (pHPPA), chavicol and Amplex red by a fluoroimmunosensing method in the use of Schistosomia japonicum antibody (SjAb) as a model analyte. The fluoroimmunosensing device was constructed by dispersing Schistosomia japonicum antigen (SjAg), nano-Ag/SiO2 particles and sol-gel at low temperature. In pH 5.8 Britton-Robinson buffer (B-R), HRP-SjAb conjugates can catalyze the polymerization reaction of RST by H2O2 forming fluorescent dimmers. The increase of the fluorescence intensity of the dimmers product at emission of 462 nm (excitation: 315 nm) is proportional to the concentration of HRP-SjAb binding to the SjAg entrapped in the nano-Ag/SiO2 particles-sol-gel matrix. A competitive binding assay has been used to determine SjAb in rabbit serum with the aid of SjAb labeled with HRP. Substrate RST showed comparable ability for HRP detection and its enzyme-linked immunosensing reaction system, in a linear detection ranging of 1.5×10-6-7.3×10-4 g/L and with a detection limit of 1.5×10-6 g/L. The immobilized biocomposites surface could be regenerated by simply polishing with an alumina paper, with an excellent reproducibility (RSD = 4.7%). The proposed method has been successfully used for analysis of the rabbit serum sample with satisfactory results.

关键词： resveratrol HRP fluorogenic substrate Ag/SiO2 nanoparticles enzyme-linked immunosensing Schistosomia japonicum

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Apolipoprotein E elicits targetdirected miRNA degradation to maintain neuronal integrity

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Neural Regeneration Research 2025年第9期20卷 2577-2578页

作者： Jiazi tan Chin-Tong Ong Temasek Life Sciences Laboratory National University of SingaporeSingapore Department of Biological Sciences National University of SingaporeSingapore

Apolipoprotein E has diverse functions in neurons:Apolipoprotein E(ApoE)is a glycoprotein that primarily regulates lipid metabolism and transport in the central nervous *** are three predominant human ApoE protein isoforms with cysteine and arginine substitutions at amino acid positions 112 and 158 that impact their lipidation and related functions(Flowers and Rebeck,2020).ApoE2 is characterized by Cys112 and Cys158,ApoE3 by Cys112 and Arg158,whereas ApoE4 contains Arg112 and Arg158.

关键词： metabolism glycoprotein maintain

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Copper-catalyzed C–H bond functionalization enabled by programmed alternating current

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Science China Chemistry 2025年第1期68卷 10-11页

作者： Zhoumei tan Kun Xu Chengchu Zeng Beijing Key Laboratory of Environmental and Viral Oncology College of Chemistry and Life Science Beijing University of Technology

Synthetic electrochemistry has been selected as one of the Top Ten Emerging Technologies in Chemistry in 2023 by International Union of Pure and Applied Chemistry (IUPAC)[1]. It has emerged as a sustainable platform for organic synthesis and has exhibited a wide range of applications from academia to industry [2]. However, electrosynthesis has been predominantly performed by using direct current (DC).

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从“货币战”到“有限合作”——1933—1936年美英法三国货币外交

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世界历史 2009年第6期 27-37页

作者：谈谭上海政法学院政治学系讲师史学博士上海201701

20世纪30年代经济大萧条背景下,美英法三国的货币政策特别是汇率政策取决于各国如何权衡国内经济恢复和国际汇率稳定。1931年英镑贬值、1933年美元贬值都是美英当局把货币贬值作为刺激总需求和出口的主要手段。金本位制的崩溃和英镑、... 详细信息

20世纪30年代经济大萧条背景下,美英法三国的货币政策特别是汇率政策取决于各国如何权衡国内经济恢复和国际汇率稳定。1931年英镑贬值、1933年美元贬值都是美英当局把货币贬值作为刺激总需求和出口的主要手段。金本位制的崩溃和英镑、美元的相继贬值使国际金融和贸易体系处于分割和对立的状态。美国对国内经济恢复的重视、英国对自治领的义务、法国对金本位的固执导致1933年三方货币合作会谈的失败。不过,随着英美两国经济的恢复,特别是希特勒德国加紧重新武装所造成的欧洲紧张局势促使美国决策层积极推动美英法三方货币合作。从货币集团分割和竞争性贬值的"货币战"发展到1936年9月后三国在汇率政策上的"有限合作",这种关系转变不仅是美国决策层"思想进展"的结果,而且是特定国际形势的产物。因此,1936年的美英法《三方货币稳定协议》具有深刻的政治含义。

关键词：货币外交 1933年伦敦三方货币会议 1936年《三方货币稳定协议》

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俄罗斯北极航道国内法规与《联合国海洋法公约》的分歧及化解途径

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上海交通大学学报（哲学社会科学版） 2017年第1期25卷 22-31页

作者：谈谭上海政法学院国际事务与公共管理学院上海201701

俄罗斯北方海航线是北极东北航道的一部分,随着全球气候变暖,北方海航线有可能成为具有重大潜在经济价值的贸易路线。冷战结束后,俄罗斯为了强化对北方海航线的控制和管理,制定了有关北方海航线的国内法规。问题是俄罗斯的相关国内法规... 详细信息

俄罗斯北方海航线是北极东北航道的一部分,随着全球气候变暖,北方海航线有可能成为具有重大潜在经济价值的贸易路线。冷战结束后,俄罗斯为了强化对北方海航线的控制和管理,制定了有关北方海航线的国内法规。问题是俄罗斯的相关国内法规与《联合国海洋法公约》存在严重分歧。这些分歧产生的原因一方面是由于两者立法旨趣不同,俄国内法规的立法宗旨是最大化其自身的国家利益,而《公约》的缔结则是平衡保障所有缔约国权益的结果;另一方面是由于国际法与国内法的优先适用问题,即俄罗斯作为《公约》缔约方,应该遵守《公约》,但其国内法规却与《公约》存在分歧并被实际执行。目前来看,化解两者分歧的途径也可能有两种:其一,俄罗斯将《公约》的相关规定内化到其国内法规中;其二,俄罗斯接受根据《公约》相关规定进行的仲裁或调解。

关键词：《联合国海洋法》北方海航线立法宗旨仲裁或调解

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