Identification of genes involved in regulating MnSOD2 production and root colonization in Bacillus cereus 905

作     者：GAO Tan-tan DING Ming-zheng LI Yan ZENG Qing-chao WANG Qi GAO Tan-tan;DING Ming-zheng;LI Yan;ZENG Qing-chao;WANG Qi

作者机构：Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural AffairsBeijing University of AgricultureBeijing 102206P.R.China Department of Plant PathologyCollege of Plant ProtectionChina Agricultural UniversityBeijing 100193P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2021年第20卷第6期

页      面：1570-1584页

核心收录：

学科分类：07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 090102[农学-作物遗传育种] 0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0836[工学-生物工程] 0713[理学-生态学] 

基　　金：the grants from the National Natural Science Foundation of China(31701860) the Program of Science and Technology of Beijing,China(Z191100004019025) 

主　　题：Bacillus cereus sod A2 TnYLB-1 transposon colonization 

摘      要：sodA2-encoding manganese-containing superoxide dismutase(MnSOD2)in Bacillus cereus 905 plays an essential role in antioxidative stress,nutrition utilization,rhizosphere and phyllosphere ***,the genes involved in regulating the sod A2 expression have not been clearly elucidated in *** this study,a genome-wide random insertion mutagenesis was constructed by using transposon TnYLB-1 to identify novel genes regulating the sodA2 *** mutants that changed the sodA2 expression at both m RNA and protein levels were finally *** analysis and BLAST data showed that the genes disrupted by TnYLB-1 in *** 905 shared high conservations with those in the *** type strain,ATCC *** genes encode heat-inducible transcription repressor,chloride channel protein,recombinase A,ferrous iron transport protein,nucleoside diphosphate kinase,and histidine ***,we also provided the evidence that the genes regulating the sodA2 expression could influence colonization ability of *** 905 on wheat ***,those genes downregulating the sodA2 expression significantly reduced bacterial colonization on wheat roots,and mutants with increased MnSOD2 activities could enhance bacterial population densities on wheat roots to a certain *** work provided information that multiple genes are involved in MnSOD2 production and wheat root *** molecular regulatory pathways through which these genes modulate the sod A2 expression and root colonization need to be investigated extensively in the future.