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Asian Pacific Journal of Tropical Biomedicine 2012年第1期2卷 61-65页

作者： tan Zi Ning Wong Weng Kin Shaymoli Mustafa Arefuddin Ahmed Rahmah Noordin tan gim cheong Olivos-Garcia Alfonso Lim Boon Huat School of Health Sciences Universiti Sains Malaysia Institute for Research in Molecular Medicine Universiti Sains Malaysia Department of Medical Microbiology ParasitologySchool of Medical SciencesUniversiti Sains Malaysia Departamento de Medicina Experimental Facultad de Medicina.Universidad National Autonoma de Mexico

Objective:To compare the efficacy of three different tissue stains,namely haematoxylin and eosin(H&E),periodic-acid Schiff(PAS)and immunohistochemical(1HC)stains for detection of Entamoeba histolytica(***)trophozoites in abscessed liver tissues of ***:Amoebic liver abscess was experimentally induced in a hamster by injecting 1×10~6of axenically cultured virulent *** trophozoites(HM1-IMSS strain)into the portal *** a week post-inoculation,the hamster was sacrificed and the liver tissue sections were stained with H&E,PAS and IHC stains to delect the amoebic ***:The three stains revealed tissue necrosis and amoebic trophozoites,but with varying clarity.H&E and PAS stained the trophozoites pink and magenta,respectively,however it was difficult to differentiate the stained trophozoites from the macrophages because of their similarity in size and *** the other hand,IHC stain revealed distinct brown appearance of the trophozoites in the infected liver ***:It can be concluded that out of the three *** is the best for identification of *** trophozoites in tissue sections.

关键词： Entamoeba histolytica Amoebic liver abscess H&E PAS IHC Trophozoite

One dimensional pea-shaped NiSe_(2) nanoparticles encapsulated in N-doped graphitic carbon fibers to boost redox reversibility in sodium-ion batteries

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Journal of Materials Science & Technology 2024年第1期168卷 215-226页

作者： Hyunjeong gim Achmad Yanuar Maulana Jiwon Choi Jungwook Song Boram Yun Yuri Jeong Nahyun An Myeongkee Park Cybelle M.Futalan Jongsik Kim Department of Chemical Engineering(BK21 FOUR Graduate Program) Dong-A UniversityBusan 49315Korea(the Republic of) Department of Chemistry Dong-A UniversityBusan 49315Korea(the Republic of) Department of Chemistry Pukyong National UniversityBusan 48513Korea(the Republic of) Institute of Civil Engineering University of the PhilippinesDiliman 1127Philippines

In recent years,sodium-ion batteries(SIBs)have emerged as a promising technology for energy storage systems(ESSs)because of the abundance and affordability of ***,metal selenides have been studied as promising high-performance conversion-type anode materials in *** them,nickel se-lenide(NiSe_(2))has received considerable attention due to its high theoretical capacity of 495 mAh g^(-1)and ***,it still suffers from poor cycling stability because of the low electrochemical reactivity,large volume expansion,and structural instability during *** address these challenges,NiSe_(2)nanoparticles encapsulated in N-doped graphitic carbon fibers(NiSe_(2)@NGCF)were synthesized by using ZIF-8 as a ***_(2)@NGCF showed a high discharge capacity of 558.3 mAh g^(-1)with a fading rate of 0.14%per cycle after 200 cycles at 0.5 A g^(-1)in 0.01-3.0 *** a very high current density of 5 A g^(-1),the capacity still displayed excellent long-term cycle life with a discharge capacity of 406.1 mAh g^(-1)with a fading rate of 0.016%per cycle after 3000 *** mechanism of the excellent electrochem-ical performance of NiSe_(2)@NGCF was thoroughly investigated by ex-situ XRD,TEM,and SEM ***,NiSe_(2)@NGCF//Na_(3)V_(2)(PO_(4))_(3)full-cell also delivered an excellent reversible capacity of 378.7 mAh g^(−1)at 0.1 A g^(−1)after 50 cycles,demonstrating its potential for practical application in SIBs.

关键词： Sodium-ion batteries Anode materials Nickel selenide N-doped graphitic carbon fibers One-dimensional electrode

Gracilaria changii(Rhodophyta) alleviates bisphenol A-induced adverse reproductive abnormalities in mice

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Asian Pacific Journal of Tropical Medicine 2021年第1期14卷 34-60页

作者： Chong Lee Ng gim cheong tan Yoon-Yen Yow Mukesh Kumar Gupta Phek Jin Kwong Department of Agricultural and Food Science Universiti Tunku Abdul Rahman31900Malaysia Department of Biomedical Science Universiti Tunku Abdul Rahman31900Malaysia Department of Biological Sciences School of Medical and Life SciencesSunway University47500Malaysia Department of Biotechnology and Medical Engineering National Institute of TechnologyRourkelaOdisha769008India

Objective:To evaluate the potential of Gracilaria changii extract in ameliorating the potential adverse effects of bisphenol ***:The antioxidant capacity of Gracilaria changii extracted using different solvents(methanol,ethanol,and aqueous)was *** mice were administered by oral gavage with bisphenol A(60 mg/kg body weight)for 6 weeks with or without Gracilaria changii aqueous ***,the mice were either euthanized for histology and immunohistochemistry studies or mated to evaluate the pregnancy ***:Gracilaria changii aqueous extract showed the highest antioxidant properties compared with extract using methanol and *** aqueous extract of Gracilaria changii improved the uterus index and uterine lipid peroxidation after bisphenol A exposure,although the uterine expressions of estrogen receptors and complement C3 were not *** evaluation of the uterus during the estrus stage has revealed that the extract could mitigate bisphenol A-induced adverse effects in the uterus as there was a lower percentage of mice showing abnormalities like decreased eosin staining in the myometrium,and decrease in the number of eosinophil and endometrial glands in the ***,Gracilaria changii aqueous extract improved the pregnancy rate of mice administered with bisphenol ***:Gracilaria changii extract protects against bisphenol A-induced female reproductive abnormalities in mice which may be mediated via modulation of eosinophil migration,endometrial gland formation,and protein expressions associated with prostaglandins in the myometrium.

关键词： Red seaweed Gracilaria changii Antioxidant Endocrine disruptor Bisphenol A Reproduction

Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation

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Journal of Animal Science and Biotechnology 2024年第1期15卷 456-462页

作者： Gyeong-Min gim Kyeong-Hyeon Eom Dong-Hyeok Kwon Dae-Jin Jung Dae-Hyun Kim Jun-Koo Yi Jae-Jung Ha Ji-Hyun Lee Seong-Beom Lee Woo-Jae Son Soo-Young Yum Won-Wu Lee Goo Jang Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical ScienceCollege of Veterinary Medicine and the Research Institute of Veterinary ScienceSeoul National UniversitySeoulRepublic of Korea LARTBio Co. Ltd.SeoulRepublic of Korea Gyeongsangbukdo Livestock Research Institute YeongjuRepublic of Korea Comparative Medicine Disease Research Center Seoul National UniversitySeoulRepublic of Korea

Background Genome editing has been considered as powerful tool in agricultural ***,genome editing progress in cattle has not been fast as in other mammal species,for some disadvantages including long gestational periods,single pregnancy,and high raising ***,technically demanding methods such as microinjection and somatic cell nuclear transfer(SCNT)are needed for gene editing in *** this point of view,electroporation in embryos has been risen as an *** First,editing efficiency of our electroporation methods were tested for *** of mutation on embryo was confirmed by T7E1 *** first combination,mutation rates for MSTN and PRNP were 57.6%±13.7%and 54.6%±13.5%,*** case of MSTN/BLG,mutation rates were 83.9%±23.6%for MSTN,84.5%±18.0%for ***,the double-KO embryos were transferred to surrogates and mutation rate was identified in resultant calves by targeted deep *** recipients were transferred for MSTN/PRNP,4 calves were delivered,and one calf underwent an induction for double *** surrogates were given double-KO embryos for MSTN/BLG,and four of the six calves that were born had mutations in both *** These data demonstrated that production of genome edited cattle via electroporation of RNP could be effectively ***,MSTN and PRNP from beef cattle and MSTN and BLG from dairy cattle have been born and they will be valuable resources for future precision breeding.

关键词： Beta-lactoglobulin Cattle CRISPR-Cas9 Electroporation Knockout MSTN PRNP

Single atom Cu-N-C catalysts for the electro-reduction of CO_(2) to CO assessed by rotating ring-disc electrode

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Journal of Energy Chemistry 2024年第1期88卷 169-182,I0004页

作者： S.Pérez-Rodríguez M.Gutiérrez-Roa C.giménez-Rubio D.Ríos-Ruiz P.Arévalo-Cid M.V.Martínez-Huerta A.Zitolo M.J.Lázaro D.Sebastián Instituto de Carboquímica(CSIC) Miguel Luesma Castán 450018 ZaragozaSpain Instituto de Catálisis y Petroleoquímica(CSIC) Marie Curie 228049 MadridSpain Synchrotron SOLEIL L’Orme des MerisiersDépartementale 12891190France

The electrochemical CO_(2) reduction reaction(CO_(2)RR) to controllable chemicals is considered as a promising pathway to store intermittent renewable energy. Herein, a set of catalysts based on copper-nitrogendoped carbon xerogel(Cu-N-C) are successfully developed varying the copper amount and the nature of the copper precursor, for the efficient CO_(2)RR. The electrocatalytic performance of Cu-N-C materials is assessed by a rotating ring-disc electrode(RRDE), technique still rarely explored for CO_(2)RR. For comparison, products are also characterized by online gas chromatography in a H-cell. The as-synthesized Cu-NC catalysts are found to be active and highly CO selective at low overpotentials(from -0.6 to -0.8 V ***) in 0.1 M KHCO_(3), while H_(2) from the competitive water reduction appears at larger overpotentials(-0.9 V vs. RHE). The optimum copper acetate-derived catalyst containing Cu-N_(4) moieties exhibits a CO_(2)-to-CO turnover frequency of 997 h^(-1) at -0.9 V vs. RHE with a H_(2)/CO ratio of 1.8. These results demonstrate that RRDE configuration can be used as a feasible approach for identifying electrolysis products from CO_(2)RR.

关键词： Cu-N-C Carbon xerogel Rotating ring disc electrode Carbon dioxide reduction reaction Carbon monoxide

Design and Analysis of Osmosis-based Artificial Muscle

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Journal of Bionic Engineering 2019年第1期16卷 56-65页

作者： Juhui gim Changsun Ahn School of Mechanical Engineering Pusan National UniversityBusan 46241Korea

This paper presents the design and analysis of an osmosis-based artificial muscle inspired by the leaf movements of Mimosa ***'s leaves quickly contract using osmosis pressure in the pulvinus when they are *** analyzed and simulated an osmosis system to identify the factors for fast osmosis reactions and designed a prototype artificial muscle based on the *** osmosis phenomenon was mathematically modeled,analyzed,and verified through several *** analysis shows that fast osmosis responses require a large diffusion coefficient with a high-flux membrane or small ratio of the cross-sectional area to the volume of the osmosis *** designed a micro-scale system to achieve the required *** contraction and relaxation of the artificial muscle are realized by changes of the local concentration of potassium ions,which can be aggregated by a controllable electric *** a result,the artificial muscle shows controllable behavior with fast reactions.

关键词： Mimosa pudica osmosis artificial muscle cross-sectional area to volume ratio micro-scale

Local Isoperimetric Inequalities for Sectors on Surfaces and Cones

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Acta Mathematica Sinica,English Series 2007年第7期23卷 1317-1326页

作者： Fernando gimNEZ Javier Orengo VALVERDE Department de Matemdtica Aplicada University Politécnica de ValenciaCamino de Vera s/n46022SPAIN Department de Matemáticas University de Castilla la ManchaAvda.de Espaas/n02071AlbaceteSpain

On surfaces we give conditions under which the solution of a restricted local isoperimetric problem for sectors with small solid angle is the circular sector and we characterize these surfaces. Also we study this prob... 详细信息

关键词： isoperimetric cones solid angle

Gut microbiota as a potential key to modulating humoral immunogenicity of new platform COVID-19 vaccines

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Signal Transduction and Targeted Therapy 2023年第6期8卷 2447-2450页

作者： Hye Seong Bo Kyu Choi Young-Hee Han Jun Hyoung Kim Jeong-An gim Sooyeon Lim Ji Yun Noh Hee Jin cheong Woo Joo Kim Joon Young Song Department of Internal Medicine Korea University College of MedicineSeoulRepublic of Korea Asia Pacific Influenza Institute Korea University College of MedicineSeoulRepublic of Korea Vaccine Innovation Center Korea University College of MedicineSeoulRepublic of Korea Department of Biomedical Systems Informatics Yonsei University College of MedicineSeoulRepublic of Korea Department of Food and Nutrition Chungbuk National UniversityCheongjuRepublic of Korea Department of Internal Medicine Chungbuk National University HospitalCheongjuRepublic of Korea Medical Science Research Center Korea University College of MedicineSeoulRepublic of Korea

Dear Editor,Coronavirus disease 2019(COVID-19)is a novel respiratory infectious disease,caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),which led to a global *** vaccination is the best measure to overcome a pandemic,the immunogenicity of vaccines can be influenced by diverse factors,including intrinsic(age,sex,genetics,and comorbidities),extrinsic(diet,nutrition,and behavior),and vaccine-associated characteristics.

关键词： vaccines respiratory acute

Regular moderate aerobic exercise improves high-fat diet-induced nonalcoholic fatty liver disease via monoacylglycerol O-acyltransferase 1 pathway suppression

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Journal of Sport and Health Science 2020年第5期9卷 472-478页

作者： Kyung-Wan Baek Jeong-An gim Jung-Jun Park Division of Sport Science Pusan National UniversityBusan 46241Korea Department of Biological Sciences College of Natural SciencesPusan National UniversityBusan 46241Korea

Purpose:Monoacylglycerol O-acyltransferase 1(MGAT1)is reported to play a key role in the development of diet-induced nonalcoholic fatty liver disease(NAFLD).Thus,this study investigated the effect of exercise on suppression of the MGAT1 pathway in NAFLD tissue of high-fat diet(HFD)-induced obese ***:Male Sprague-Dawley rats were fed an HFD containing 45%fat for 6 *** confirmation that NAFLD had been induced in the obese animals,they were divided into HFD-fed groups provided with exercise(HFD+EXE)or without exercise(HFD)and a group given dietary adjustment(DA)only,for a further 6 weeks of intervention *** 6-week regular moderate aerobic exercise consisted of an accommodation phase with increasing *** accumulation in the liver tissue was determined by Oil Red O *** MGAT1 and liver lipogenic gene mRNA levels were measured by qPCR,and their protein levels by western blot ***:Oil Red O staining showed that NAFLD was successfully induced by *** gene expression of MGAT1 was significantly lower in HFD+EXE than ***,there was no significant difference between HFD+EXE and *** protein expression of MGAT1 was significantly lower in HFD+EXE than both HFD and *** RNA and protein expression of other lipogenic genes were not different among *** data indicate that exercise suppresses MGAT1 pathway regardless of HFD feeding;in part,this effect could be greater than ***:Our data suggest that exercise can improve NAFLD,which is probably due to suppression of MGAT1 pathway.

关键词： Exercise High-fat diet Monoacylglycerol O-acyltransferase 1 Nonalcoholic fatty liver disease Obesity

Provisional Stenting:A Contemporary Relook at the Strategy and Lingering Issues

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Cardiology Discovery 2022年第3期2卷 191-196页

作者： gim-Hooi Choo Kumara Gurupparan Cardiac Vascular Sentral Kuala Lumpur Kuala Lumpur 50470Malaysia Institut Jantung Negara Kuala Lumpur 50400Malaysia

Bifurcation lesions are encountered and treated in up to 1 in 5 cases of percutaneous coronary intervention(PCI).Such lesions pose a technical challenge to PCI,leading to lower procedural success and a higher rate of long-term adverse ***,each bifurcation is unique in terms of anatomy and pathological *** is no“one size fits all”strategy for coronary bifurcation ***,in most scenarios,provisional stenting is the preferred *** method is easy to apply and involves a logical stepwise escalation approach that is highly successful and safe.

关键词： Percutaneous coronary intervention Coronary bifurcation Provisional stenting Proximal optimization technique Kissing balloon inflation