Detection of Entamoeba histolytica in experimentally induced amoebic liver abscess:comparison of three staining methods

作     者：Tan Zi Ning Wong Weng Kin Shaymoli Mustafa Arefuddin Ahmed Rahmah Noordin Tan Gim Cheong Olivos-Garcia Alfonso Lim Boon Huat 

作者机构：School of Health SciencesUniversiti Sains Malaysia Institute for Research in Molecular MedicineUniversiti Sains Malaysia Department of Medical MicrobiologyParasitologySchool of Medical SciencesUniversiti Sains Malaysia Departamento de Medicina ExperimentalFacultad de Medicina.Universidad National Autonoma de Mexico 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2012年第2卷第1期

页      面：61-65页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by a grant from Universiti Sains Malaysia(grant No.1001/PPSK/813009) received financial support through the USM Fellowship 

主　　题：Entamoeba histolytica Amoebic liver abscess H&E PAS IHC Trophozoite 

摘      要：Objective:To compare the efficacy of three different tissue stains,namely haematoxylin and eosin(H&E),periodic-acid Schiff(PAS)and immunohistochemical(1HC)stains for detection of Entamoeba histolytica(***)trophozoites in abscessed liver tissues of ***:Amoebic liver abscess was experimentally induced in a hamster by injecting 1×10~6of axenically cultured virulent *** trophozoites(HM1-IMSS strain)into the portal *** a week post-inoculation,the hamster was sacrificed and the liver tissue sections were stained with H&E,PAS and IHC stains to delect the amoebic ***:The three stains revealed tissue necrosis and amoebic trophozoites,but with varying clarity.H&E and PAS stained the trophozoites pink and magenta,respectively,however it was difficult to differentiate the stained trophozoites from the macrophages because of their similarity in size and *** the other hand,IHC stain revealed distinct brown appearance of the trophozoites in the infected liver ***:It can be concluded that out of the three *** is the best for identification of *** trophozoites in tissue sections.