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Purification of enzymatically inactive peptidylarginine deiminase type 6 from mouse ovary that reveals hexameric structure different from other dimeric isoforms

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Advances in Bioscience and Biotechnology 2011年第4期2卷 304-310页

作者： Hirofumi taki Tomoharu gomi Bryan Knuckley Paul R. Thompson Oliver Vugrek Kazuya Hirata Tatsurou Miyahara Kouichiro Shinoda Hiroyuki Hounoki Eiji Sugiyama Isao Usui Masaharu Urakaze Kazuyuki Tobe Tetsuya Ishimoto Ran Inoue Ayumi Tanaka Hiroki Mano Hirofumi Ogawa Hisashi Mori 不详

The murine peptidylarginine deiminase (PAD) has five isoforms encoded by different genes and participates in a variety of cellular functions through the citrullination of target proteins. The crystal structure of human PAD4 with a dimeric form was previously solved because of the enzyme’s relevance to rheumatoid arthritis. PAD6, abundant in mouse oocytes and eggs, is believed to take part in early events of embryogenesis, but its biochemical properties are little understood. Here we have purified and characterized a recombinant PAD6. A PAD6 cDNA was cloned from mouse ovary RNA and expressed in Escherichia coli through pET29 and pgEX vectors. When benzoyl-L-arginine ethyl ester was used as a substrate, no appreciable activity was detected with a cell homogenate under conditions where a human PAD4 cDNA caused significant activity. Both proteins were affinity-purified to near homogeneity. The circular dichroism spectra of PAD6 and human PAD4 were similar in the far ultraviolet region. On molecular sieving, PAD6 was eluted faster than human PAD4. The cross-linking of PAD6 with dimethyl suberimidate clearly showed six bands on an sodium dodecyl sulfate-polyacrylamide gel. These results indicate that PAD6 can constitute a hexameric structure. The purified PAD6 still showed no enzymatic activity. This unique structure and loss in enzymatic activity is strongly suggested to favor the formation of egg cytoplasmic sheets as the architectural protein.

关键词： Peptidylarginine deiminase Isoform Dimer Hexamer Mouse Oocytes Cytoplasmic sheets.

Treatment of Asthma in the Elderly: Questionnaire Survey in Japan

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International Journal of Clinical Medicine 2017年第4期8卷 236-247页

作者： Sahoko Chiba Kimitake Tsuchiya Tomoyuki Ogata Reina Imase Tamon Yagi Yuka Mishima Torahiko Jinta Kazuhito Saito Reiko taki Susumu Isogai Yasuto Jinn Tsutomu Kawasaki Ichiro Natsume Yoshihiro Miyashita Jun Takagiwa Nobuo Ishiwata Tomoshige Chiaki Masato Kishi Yoshikazu Tsukada Motohisa Yamasaki Naohiko Inase Department of Respiratory Medicine Tokyo Medical and Dental University Tokyo Japan JA Toride Medical Center Ibaraki Japan St. Luke’s International Hospital Tokyo Japan Tsuchiura Kyoudou general Hospital Ibaraki Japan Japanese Red Cross Musashino Hospital Tokyo Japan Ome Municipal general Hospital Tokyo Japan Hiratsuka Kyosai Hospital Kanagawa Japan Yokohama City Minato Red Cross Hospital Kanagawa Japan Yokosuka Kyosai Hospital Kanagawa Japan Yamanashi Prefectural Central Hospital Yamanashi Japan Tokyo Kyosai Hospital Tokyo Japan Kudanzaka Hospital Tokyo Japan Hokushin general Hospital Nagano Japan Mishima general Hospital Shizuoka Japan Soka Municipal Hospital Saitama Japan Nitobe Memorial Nakano general Hospital Tokyo Japan

Introduction: The prevalence of asthma in the elderly has been increasing due to the aging of the global population. Appropriate treatment for asthma in the elderly is now a major issue, as the disease is often fatal and incurs high medical costs in the elderly population. There have been few recent reports on asthma treatment for the elderly in the real-world clinical settings. Objectives: This study was performed to assess how respiratory physicians manage asthma in elderly patients in clinical settings in Japan. Methods: Respiratory physicians in 16 Japanese hospitals responded to a questionnaire survey on asthma and COPD treatment between December 2014 and February 2015. The analysis was performed using data collected from medical records on 2041 asthma patients. Results: The mean patient age was 61.2 years, and 36.6% of the patients were men. In the breakdown by age, 1018 (49.9%) of the patients were elderly (≥ 65 years). Compared to the non-elderly, the elderly patients had a significantly lower BMI, greater smoking history in pack-years, and poorer pulmonary function. There were no significant differences between the elderly and non-elderly in the prescription rates of common medications such as inhaled corticosteroids, long-acting beta-agonists (LABA) administered via inhaler or transdermal patch, long-acting muscarinic antagonists, or leukotriene receptor antagonists. In contrast, mucoactive drugs (18.7% vs. 12.3%, P P P = 0.02). Conclusions: According to this survey, the prescription rates of mucoactive drugs, macrolides, and transdermal LABA were significantly higher in the elderly than in the non-elderly.

关键词： Asthma Elderly Transdermal LABA Aging

利用图象处理技术对船体周围波分布的测量实验与研究

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哈尔滨工程大学学报 2001年第1期22卷 1-6页

作者：黄胜吴念东奥野武俊姬野洋司哈尔滨工程大学黑龙江哈尔滨150001 大阪府立大学

提供了利用图象处理技术在曳航水池中测量船体周围波高分布的测量系统及其理论分析 .当镭射光平面从曳航水池中射出 ,根据画像上的明暗边界对自由表面进行了可视化 .结合可视化分析和图象处理技术 ,本文开发了一种对静水和规则波中船体... 详细信息

提供了利用图象处理技术在曳航水池中测量船体周围波高分布的测量系统及其理论分析 .当镭射光平面从曳航水池中射出 ,根据画像上的明暗边界对自由表面进行了可视化 .结合可视化分析和图象处理技术 ,本文开发了一种对静水和规则波中船体周围波高分布进行测量的图象测量系统 .在曳航水池中为了在图象中获得足够的明暗对比度 ,需要使用高性能镭射发光器和高清晰度的摄像机 .测量解析结果显示本系统不仅适用一般性船而且适用于高速船 .还介绍了一种新的对船体周围波高分布测量的实验结果的分析方法 .该方法的数值计算采用了广义的最小二乘法 .文中提供的测量系统和分析方法对波浪阻力研究和流场模型构造有一定的实用价值 .

关键词：图像测量流体可视化阻力分析波高分布图象处理船体周围

Diagnosis of small intramucosal signet ring cell carcinoma of the stomach by non-magnifying narrow-band imaging: A pilot study

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World Journal of gastrointestinal Endoscopy 2015年第12期7卷 1070-1077页

作者： Jiro Watari Toshihiko Tomita Hisatomo Ikehara Masato taki Tomohiro Ogawa Takahisa Yamasaki Takashi Kondo Fumihiko Toyoshima Jun Sakurai Tomoaki Kono Katsuyuki Tozawa Yoshio Ohda Tadayuki Oshima Hirokazu Fukui Seiichi Hirota Hiroto Miwa Division of gastroenterology Department of Internal Medicine Hyogo College of Medicine NishinomiyaHyogo 663-8501 Japan Department of Surgical Pathology Hyogo College of Medicine Nishinomiya Hyogo 663-8501 Japan Division of gastroenterology Department of Internal Medicine Hyogo College of MedicineNishinomiyaHyogo 663-8501 Japan

AIM: To examine the efficacy of non-magnifying narrow-band imaging(NM-NBI) imaging for small signet ring cell carcinoma(SRC).METHODS: We retrospectively analyzed 14 consecutive small intramucosal SRCs that had been treated with endoscopic submucosal dissection(ESD) and 14 randomly selected whitish gastric ulcer scars(control). The strength and shape of the SRCs and whitish scars by NM-NBI and white-light imaging(WLI) were assessed with Image J(NIH, Bethesda).RESULTS: NM-NBI findings of SRC showed a clearly isolated whitish area amid the brown color of the surrounding normal mucosa. The NBI index, which indicates the potency of NBI for visualizing SRC, was significantly higher than the WLI index(P = 0.001), indicating SRC was more clearly identified by NM-NBI. Although the NBI index was not significantly different between SRCs and controls, the circle(C)-index, as an index of circularity of tumor shape, was significantly higher in SRCs(P = 0.001). According to the receiveroperating characteristic analysis, the resulting cut-off value of the circularity index(C-index) for SRC was 0.60(85.7% sensitivity, 85.7% specificity). Thus a lesion with a C-index ≥ 0.6 was significantly more likely to be an SRC than a gastric ulcer scar(OR = 36.0; 95%CI: 4.33-299.09; P = 0.0009).CONCLUSION: Small isolated whitish round area by NM-NBI endoscopy is a useful finding of SRCs which is the indication for ESD.

关键词： gastric cancer Signet ring cell carcinoma Narrow-b

Histological diagnosis of gastric submucosal tumors: A pilot study of endoscopic ultrasonography-guided fine-needle aspiration biopsy vs mucosal cutting biopsy

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World Journal of gastrointestinal Endoscopy 2015年第14期7卷 1142-1149页

作者： Hisatomo Ikehara Zhaoliang Li Jiro Watari Masato taki Tomohiro Ogawa Takahisa Yamasaki Takashi Kondo Fumihiko Toyoshima Tomoaki Kono Katsuyuki Tozawa Yoshio Ohda Toshihiko Tomita Tadayuki Oshima Hirokazu Fukui Ikuo Matsuda Seiichi Hirota Hiroto Miwa Division of gastroenterology Department of Internal Medicine Hyogo College of Medicine Department of Surgical Pathology Hyogo College of Medicine

AIM: To compare the usefulness of endoscopic ultrasonography-guided fine-needle aspiration biopsy(EUS-FNAB) without cytology and mucosal cutting biopsy(MCB) in the histological diagnosis of gastric submucosal tumor(SMT).METHODS: We prospectively compared the diagnostic yield, feasibility, and safety of EUS-FNAB and those of MCB based on endoscopic submucosal dissection. The cases of 20 consecutive patients with gastric SMT ≥1 cm in diameter. who underwent both EUS-FNAB and MCB were ***: The histological diagnoses were gastrointestinal stromal tumors(n = 7), leiomyoma(n =6), schwannoma(n = 2), aberrant pancreas(n = 2), and one case each of glomus tumor, metastatic hepatocellular carcinoma, and no-diagnosis. The tumors' mean size was 23.6 mm. Histological diagnosis was made in 65.0% of the EUS-FNABs and 60.0% of the MCBs, a nonsignificant difference. There were no significant differences in the diagnostic yield concerning the tumor location or tumor size between the two methods. However, diagnostic specimens were significantly more frequently obtained in lesions with intraluminal growth than in those with extraluminal growth by the MCB method(P = 0.01). All four SMTs with extraluminal growth were diagnosed only by EUSFNAB(P = 0.03). No complications were found in either ***: MCB may be chosen as an alternative diagnostic modality in tumors showing the intraluminal growth pattern regardless of tumor size, whereas EUSFNAB should be performed for SMTs with extraluminal growth.

关键词： Submucosal tumor Endoscopic ultrasonography-guided

蛛网膜下腔出血后症状性血管痉挛的检测:单次和连续动脉自旋标记的初步应用

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国际医学放射学杂志 2013年第1期 85-85页

作者： K.Aoyama Y.Fushimi T.Okada A.Miyasaki H.taki K.Shibamoto 路丽 Department of Radiology Ichinomiyanishi HospitalKaimeiIchinomiya 494-0001Japan

目的采用动脉自旋标记(ASL)检测蛛网膜下腔出血术后的症状半球。方法研究包括18例动脉瘤所致蛛网膜下腔出血的病人,4例术后出现症状性血管痉挛。全部病人在第9～10天进行ASL检查(单次ASL)。9例病人分别在第1～2天、9～10天和13～21天进... 详细信息

目的采用动脉自旋标记(ASL)检测蛛网膜下腔出血术后的症状半球。方法研究包括18例动脉瘤所致蛛网膜下腔出血的病人,4例术后出现症状性血管痉挛。全部病人在第9～10天进行ASL检查(单次ASL)。9例病人分别在第1～2天、9～10天和13～21天进行连续ASL检查(连续ASL),7例病人在第4～7天也进行了检查。测量大脑中动脉供血区后部的脑血流量(CBF),估算同侧/对侧的CBF比例。通过受试者操作特征(ROC)曲线分析症状半球和其他部位之间的差异。结果单次ASL显示,CBF第9～10天和CBF同侧/对侧第9～10天在症状半球明显低于非症状半球(P<0.001)。连续ASL显示,CBF第4～7天较CBF第1～2天明显降低,CBF第9～10天较CBF第4～7天明显降低,而CBF第13～21天较CBF第9～10天明显升高。单次ASL的ROC分析显示CBF第9～10天的曲线下面积为0.95,明显高于CBF同侧/对侧第9～10天(P<0.001)。连续ASL的ROC分析显示CBF第9～10天的曲线下面积为0.93,明显高于CBF第9～10天/第1～2天和CBF同侧/对侧第9～10天(P<0.001)。结论单次ASL显示症状半球较非症状半球CBF明显减低。连续ASL可显示蛛网膜下腔出血后CBF随时间的变化。

关键词：蛛网膜下腔出血 ASL 血管痉挛症状性单次