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Rotation of Biological Cells:Fundamentals and Applications

Engineering 2022年第3期8卷 110-126页

作者： Tao Tang Yoichiroh Hosokawa takeshi hayakawa Yo Tanaka Weihua Li Ming Li Yaxiaer Yalikun Division of Materials Science Nara Institute of Science and TechnologyNara 630-0192Japan Institute of Innovation for Future Society Nagoya UniversityAichi 464-8603Japan Department of Precision Mechanics Faculty of Science and EngineeringChuo UniversityTokyo 112-8551Japan Graduate School of Frontier Biosciences Osaka UniversityOsaka 565-0871Japan RIKEN Center for Biosystems Dynamics Research Osaka 565-0871Japan School of Mechanical MaterialsMechatronicand Biomedical EngineeringUniversity of WollongongWollongongNSW 2522Australia School of Engineering Macquarie UniversitySydneyNSW 2109Australia

Cell rotation is one of the most important techniques for cell manipulation in modern bioscience,as it not only permits cell observation from any arbitrary angle,but also simplifies the procedures for analyzing the mechanical properties of cells,characterizing cell physiology,and performing *** approaches have been reported for rotating cells in a wide range of academic and industrial *** them,the most popular are micro-robot-based direct contact manipulation and field-based non-contact methods(e.g.,optical,magnetic,electric,acoustic,and hydrodynamic methods).This review first summarizes the fundamental mechanisms,merits,and demerits of these six main groups of approaches,and then discusses their differences and limitations in *** aim to bridge the gap between each method and illustrate the development progress,current advances,and prospects in the field of cell rotation.

关键词： Cell rotation Cell reorientation Micromanipulation Microfluidics

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On-chip 3D rotation of oocyte based on a vibration-induced local whirling flow

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Microsystems & Nanoengineering 2015年第1期1卷 150-158页

作者： takeshi hayakawa Shinya Sakuma Fumihito Arai Department of Micro-Nano Systems Engineering Nagoya UniversityNagoya-CityAichi-Pref.JAPAN

We propose a novel on-chip 3D cell rotation method based on a vibration-induced *** circular vibration is applied to a microchip with micropillar patterns,a highly localized whirling flow is induced around the *** direction and velocity of this flow can be controlled by changing the direction and amplitude of the applied ***,this flow can be induced on an open chip *** this study,we adopted a microchip with three micropillars arranged in a triangular configuration and an xyz piezoelectric actuator to apply the circular *** the centre of the micropillars,the interference of the vibration-induced flows originating from the individual micropillars induces rotational ***,a biological cell placed at this centre rotates under the influence of the *** three-plane circular vibrations in the xy,xz or yz plane,the cell can rotate in both the focal and vertical planes of the *** this 3D cell rotation method,we measured the rotational speeds of mouse oocytes in the focal and vertical planes as 63.7±4.0°s^(−1) and 3.5±2.1°s^(−1),***,we demonstrated the transportation and rotation of the mouse oocytes and re-positioned their nuclei into a position observable by microscope.

关键词： lab on a chip cell manipulation vibration-induced flow acoustic streaming

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An Evans Syndrome Case Expressing Anti-Jk<sup>a</sup>Autoantibody under Condition of Primary IgA Immunodeficiency

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International Journal of Clinical Medicine 2015年第7期6卷 496-499页

作者： takeshi Sugimoto Ayumi Tani Ikuyo hayakawa Makoto Hashimoto Tomoko Yanai Ikuko Kubokawa takeshi Mori takeshi Kato Satoshi Hirase Nobuyuki Yamamoto Hironobu Minami Akira hayakawa Department of Hematology and Oncology Kita-Harima Medical Center Ono Japan Department of Blood Transfusion Kobe University Hospital Kobe Japan Institute of Biomedical Research and Innovation Kobe Japan Department of Pediatric Medicine Kobe University Hospital Kobe Japan

Autoimmune haemolytic anaemia is a haemolytic disease resulting from an autoimmune reaction to the surface of red blood cells. A part of autoantibody is known to react with the blood type antigen. This is the case of a 14 years old female with Evans syndrome in which autoimmune haemolysis may cause from anti-Jka autoantibody reaction. As this case is complicated with primary IgA immunodeficiency syndrome, anti-Jka autoantibody may occur under the condition of primary immunodeficiency status, in which autoantibody production is accelerated. Considering the co-occurrence of autoimmune haemolytic anaemia and primary IgA immunodeficiency syndrome, analysis focusing on specificity for red blood cells antigens will be required in IgA immunodefi-ciency syndrome patients.

关键词： Anti-Jka Autoantibody Evans Syndrome IgA Immunodeficiency

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A case of toxic epidermal necrolysis complicated by severe liver damage and hemophagocytic syndrome of unknown pathogenesis

A case of toxic epidermal necrolysis complicated by severe l...

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The Second Eastern Asia Dermatology Congress(EADC2012)

作者： Toshifumi Yamaoka Hiroaki Azukizawa Atsushi Tanemura Hiroyuki Murota Tomoya Hirose Koichi hayakawa takeshi Shimazu Naoki Wada Eiichi Morii Ichiro Katayama Department of Dermatology Course of Integrated MedicineOsaka University Graduate School of Medicine Department of Traumatology and Acute Critical Medicine Osaka University Graduate School of Medicine Department of Pathology Osaka University Graduate School of Medicine

＜正＞A 76-year-old Japanese woman who had received Etodolac for cervical spondylosis over the previous 2 weeks, developed a cough and a sore throat associated with common cold in the previous 10 *** consulted out-patient clinic of other hospital and admitted with the findings of severe liver *** was found to have confluent targetoid

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