On-chip 3D rotation of oocyte based on a vibration-induced local whirling flow

作     者：Takeshi Hayakawa Shinya Sakuma Fumihito Arai 

作者机构：Department of Micro-Nano Systems EngineeringNagoya UniversityNagoya-CityAichi-Pref.JAPAN 

出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)

年 卷 期：2015年第1卷第1期

页      面：150-158页

核心收录：

学科分类：08[工学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：This study was financially supported by Grant-in-Aid for JSPS Fellows Number 13J03580 Grant-in-Aid for Scientific Research on Innovative Areas(No.23106002) No.26630094 

主　　题：lab on a chip cell manipulation vibration-induced flow acoustic streaming 

摘      要：We propose a novel on-chip 3D cell rotation method based on a vibration-induced *** circular vibration is applied to a microchip with micropillar patterns,a highly localized whirling flow is induced around the *** direction and velocity of this flow can be controlled by changing the direction and amplitude of the applied ***,this flow can be induced on an open chip *** this study,we adopted a microchip with three micropillars arranged in a triangular configuration and an xyz piezoelectric actuator to apply the circular *** the centre of the micropillars,the interference of the vibration-induced flows originating from the individual micropillars induces rotational ***,a biological cell placed at this centre rotates under the influence of the *** three-plane circular vibrations in the xy,xz or yz plane,the cell can rotate in both the focal and vertical planes of the *** this 3D cell rotation method,we measured the rotational speeds of mouse oocytes in the focal and vertical planes as 63.7±4.0°s^(−1) and 3.5±2.1°s^(−1),***,we demonstrated the transportation and rotation of the mouse oocytes and re-positioned their nuclei into a position observable by microscope.