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Differential Responses Between Monocytes and Monocyte-Derived Macrophages for Lipopolysaccharide Stimulation of Calves

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Cellular & Molecular Immunology 2009年第3期6卷 223-229页

作者： Yijie Guo Guoqi Zhao Sachi Tanaka takahiro yamaguchi Laboratory of animal nutrition Graduate School of Animal Science and Technology Yangzhou University Wen Hui Road Jiangsu 225009 China Laboratory of Functional Morphology Graduate School of Agricultural Science Tohoku University 1-1 Tsutsumidori-Amamiyamachi Aobaku Sendai 981-8555 Japan

In this experiment Toll-like receptor expression pattern in monocytes and monocyte-derived macrophages by lipopolysaccharide （LPS） stimulation was examined. Jugular venous blood was collected from four Japanese calves, and the peripheral blood mononuclear cells （PBMCs） were isolated. The cells were directly used for collecting monocytes by magnetic cell sorting or cultured for 7 days to collect monocyte-derived macrophages in Repcell. Then we analyzed the mRNA expression pattern of TLRs and cytokines in monocytes and monocyte-derived macrophages after LPS stimulation for 24 h. LPS stimulation of both monocytes and monocyte-derived macrophages resulted in an increase in the levels of mRNA transcripts for TNF-α IL-6 and IL-8. Moreover, TNF-α and IL-6 mRNA expressions were significantly augmented by LPS stimulation in monocyte-derived macrophages. TLRs mRNA expressions were unchanged after LPS stimulation of monocytes, while TLRs mRNA expressions in monocyte-derived macrophages were complicated. TLR1, 3, 5, 8 and 10 were significantly decreased after LPS stimulation and there were no differences in the mRNA expressions of TLR2, 4, 6 and 7 between the groups of control and LPS stimulation. Besides, no expression of TLR9 was found. As antigen presenting cells, monocytes and monocyte-derived macrophages respond differently to LPS, so they may have different functions in the innate immune system.

关键词： Toll-like receptor monocyte monocyte-derived macrophage bovine

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Xanthogranulomatous cholecystitis mimicking gallbladder carcinoma with a false-positive result on fluorodeoxyglucose PET

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World Journal of Gastroenterology 2009年第29期15卷 3691-3693页

作者： Isamu Makino takahiro yamaguchi Nariatsu Sato Toshiaki Yasui Ichiro Kita Department of Surgery Kanazawa Social Insurance Hospital Kanazawa Ishikawa 920-8610 Japan

Recently, several reports have demonstrated that fluorine-18 fluorodeoxyglucose positron emission tomography (FDG-PET) is useful in differentiating between benign and malignant lesions in the gallbladder. However, there is a limitation in the ability of FDG-PET to differentiate between inflammatory and malignant lesions. We herein present a case of xanthogranulomatous cholecystitis misdiagnosed as gallbladder carcinoma by ultrasonography and computed tomography. FDG-PET also showed increased activity. In this case, FDG-PET findings resulted in a false-positive for the diagnosis of gallbladder ***, several reports have demonstrated that fluorine-18 fluorodeoxyglucose positron emission tomography (FDG-PET) is useful in differentiating between benign and malignant lesions in the gallbladder. However, there is a limitation in the ability of FDG-PET to differentiate between inflammatory and malignant lesions. We herein present a case of xanthogranulomatous cholecystitis misdiagnosed as gallbladder carcinoma by ultrasonography and computed tomography. FDG-PET also showed increased activity. In this case, FDG-PET findings resulted in a false-positive for the diagnosis of gallbladder carcinoma.

关键词：胆囊炎肉芽肿阳性 FDG PET 断层扫描黄色癌

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The TLR Expression Pattern on Monocyte-Derived Macrophages for Lipopolysaccharid Stimulation of Calves

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Agricultural Sciences in China 2009年第7期8卷 864-871页

作者： GUO Yi-jie ZHAO Guo-Qi HUO Yong-jiu Sachi Tanaka Hisashi Aso takahiro yamaguchi Laboratory of Animal Nutrition Graduate School of Animal Science and Technology Yangzhou University Yangzhou 225009 P.R. China Laboratory of Functional Morphology Graduate School of Agricultural Science Tohoku University 1-1 Tsutsumidori-Amamiyamachi Aobaku 981-8555 Sendai Japan

In this paper, toll-like receptor expression pattern in monocytes-derived macrophages by lipopolysaccharid （LPS） stimulation was examined. Jugular venous blood samples from 4 Japanese calves were obtained and the peripheral blood mononuclear cells （PBMC） were isolated. The PBMC were cultured for 7 d so as to collect monocytes-derived macrophages in Repcell. The PBMC were stimulated by LPS for 24 h and the mRNA expression pattern of TLR and cytokines in monocytes-derived macrophages （Mod-Mφ） was analyzed. Results showed that LPS stimulation of Mod-Mφ could increase the mRNA levels of the genes of TNF-α, IL-6, and IL-8. In addition, the mRNA levels of the genes of TNF-α and IL-6 in the group of LPS stimulation were most significantly （P 〈 0.01） higher than those in control group and the mRNA levels of TLR1, 3, 5, 8, and 10 were significantly （P 〈 0.05） decreased after LPS stimulation. There was no difference in the mRNA expressions of TLR2, 4, 6, and 7 between the groups of the control and LPS stimulation. Besides, expression of TLR9 was not found. It suggested that monocytes-derived macrophages could respond to LPS and they might take an important role in the innate immunity. The important function of the cells might contribute to better disease treatment.

关键词： TLR bovine macrophage LPS

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Abdominal Aortic Aneurysm Repair in a Patient with Superior Mesenteric Artery Occlusion with Transient Intraoperative Infusion of the Inferior Mesenteric Artery: A Surgical Case Report

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World Journal of Cardiovascular Surgery 2018年第11期8卷 213-218页

作者： Yasuhiko Kobayashi takahiro yamaguchi Junichi Yoshida Department of Cardiovascular Surgery Amagasaki Central Hospital Amagasaki Japan Department of Cardiovascular Surgery Kawachi General Hospital Osaka Japan Department of Cardiology Amagasaki Central Hospital Amagasaki Japan

Abdominal aortic aneurysms (AAA) with mesenteric artery stenosis or obstructive lesions are occasionally recognized. While performing the graft replacement operation in such cases, if the collateral circulation supplying the gastrointestinal tract is well developed, it is necessary that this collateral circulation should be well perfused during aortic cross-clamping. A 72-year-old woman was admitted because of expansion of AAA. Computed tomography (CT) scan examination revealed occlusion at the origin of the superior mesenteric artery (SMA) and the development of a collateral circulatory pathway via the inferior mesenteric artery (IMA). During this operation, a vein cannula was placed in the IMA, through which blood was supplied with an extension tube for the protection of the intra-abdominal organs. The IMA was reimplanted after the graft replacement. Previous reports indicate that intestinal ischemia may be successfully prevented by supplying blood to the collateral circulation using various techniques. We report the surgical approach for treatment of AAA using a simple and convenient method to maintain intra-operative blood supply to vital organs.

关键词： Abdominal Aortic Aneurysm Occlusion of the Superior Mesenteric Artery Transient Perfusion Simple Method

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Performance of Route-Information Based Prioritization （RIBP） for ATM Networks

Performance of Route-Information Based Prioritization （RIBP...

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2000 International Conference on Communication Technology(WCC2000·ICCT2000)

作者： takahiro yamaguchi Shinji SUGAWARA Tetsuya MIKI Department of Information and Communication Eng.University of Electro-Communications 1-5-1 Chofugaoka Chofu-shiTokyo 182-8585Japan

＜正＞Route-Information Based Prioritization（RIBP） scheme per VC（Virtual Connection） on ATM networks aims to improve both the total and minimum transmission-efficiency by reducing the total number of incomplete transmission cells（or packets）,especially for data transmission. RIBP gives the priority weighted by routeinformation -the location of nodes which make a connection,and cell drop probability on those nodes-for each VC,and operates prioritized queueing at output buffers with those *** simulated RIBP scheme on some network models and examined its *** also made a prototype which implemented RIBP for ATM networks,and confirmed its behavior.

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TLRS EXPRESSION PATTERN IN MONOCYTES AND MONOCYTE DERIVED MACROPHAGES OF CALVES

TLRS EXPRESSION PATTERN IN MONOCYTES AND MONOCYTE DERIVED MA...

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中国畜牧兽医学会动物营养学分会第八届全国会员代表大会暨...

作者： Tanaka Sachi Watanabe Kouichi Ohwada Shyuichi Aso Hisashi yamaguchi takahiro College of of life science ohoku UniversityJapan College of of life science Tohoku UniversityJapan

＜正＞Monocytes and macropHages have numerous important functions on the immune defense system,because they phagocytose foreign antigens,regulate immunocompetent cells and produce several kinds of *** innate immunity that is crucial for more rapid defense mechanisms is initiated by the recognition of pathogens on germ-line encoded pattern recognition *** recognition is mainly attributed to the family of Toll-like re-

关键词： Expression Monocytes Monocyte Calves

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Genetic Characterization of Streptococcus pyogenes emm89 Strains Isolated in Japan From 2011 to 2019

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Infectious Microbes & Diseases 2020年第4期2卷 160-166页

作者： Yujiro Hirose Masaya yamaguchi Norihiko Takemoto Tohru Miyoshi-Akiyama Tomoko Sumitomo Masanobu Nakata Tadayoshi Ikebe Tomoki Hanada takahiro yamaguchi Ryuji Kawahara Rumi Okuno Hitoshi Otsuka Yuko Matsumoto Yuji Terashima Yu Kazawa Noriko Nakanishi Kaoru Uchida Yumi Akiyama Kaori Iwabuchi Chikara Nakagawa Kazunari Yamamoto Victor Nizet Shigetada Kawabata Department of Oral and Molecular Microbiology Osaka University Graduate School of DentistrySuitaOsakaJapan Department of Pediatrics University of California at San Diego School of MedicineLa JollaCAUSA Department of Infectious Diseases Research InstituteNational Center for Global Health and MedicineTokyoJapan Department of Oral Microbiology Kagoshima University Graduate School of Medical and Dental SciencesKagoshimaJapan Department of Bacteriology I National Institute of Infectious DiseasesTokyoJapan Division of Microbiology Osaka Institute of Public HealthOsaka CityOsakaJapan Department of Microbiology Tokyo Metropolitan Institute of Public HealthTokyoJapan Department of Public Health Sciences Yamaguchi Prefectural Institute of Public Health and Environment Yamaguchi CityYamaguchiJapan Microbiological Testing and Research Division Yokohama City Institute of Public HealthYokohamaKanagawaJapan Department of Microbiology Fukushima Prefectural Institute of Public HealthFukushima CityFukushimaJapan Department of Infectious Diseases Kobe Institute of HealthKobeHyogoJapan Department of Bacteriology Toyama Institute of HealthImizuToyamaJapan Infectious Disease Research Division Hyogo Prefectural Institute of Public Health ScienceKakogawaHyogoJapan Department of Health Science Iwate Prefectural Research Institute for Environmental Sciences and Public HealthMoriokaIwateJapan Division of Microbiology Kyoto City Institute of Health and Environmental SciencesKyoto CityKyotoJapan Niigata City Institute of Public Health and the Environment Niigata CityNiigataJapan Skaggs School of Pharmaceutical Sciences University of California at San DiegoLa JollaCAUSA.

Invasive infection caused by Streptococcus pyogenes emm89 strains has been increasing in several countries linked to a recently emergent clade of emm89 strains,designated clade *** Japan,the features of emm89 *** strains,such as clade classification,remains *** this study,we collected emm89 strains isolated from both streptococcal toxic shock syndrome(STSS)(89 STSS isolates)and noninvasive infections(72 non-STSS isolates)in Japan from 2011 to 2019,and conducted wholegenome sequencing and comparative analysis,which resulted in classification of a large majority into clade 3 regardless of disease *** addition,invasive disease-associated factors were found among emm89 strains,including mutations of control of virulence sensor,and absence of the hylP1 gene encoding *** findings provide new insights into genetic features of emm89 strains.

关键词： clade 3 emm89 streptococcal toxic shock syndrome Streptococcus pyogenes

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Angioscopic Observation of an Endoluminal Stent Graft: CT Imaging versus Angioscopic Imaging

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World Journal of Cardiovascular Surgery 2016年第6期6卷 87-92页

作者： Yasuhiko Kobayashi takahiro yamaguchi Sei Komatsu Tomoki Ohara Junichi Yoshida Mitsuhiko Takewa Satoru Takahashi Chikao Yutani Kazuhisa Kodama Shizuo Yoshida Departments of Cardiovascular Surgery Amagasaki Central Hospital Hyogo Japan Departments of Cardiovascular Surgery Kawachi General Hospital Osaka Japan Departments of Cardiology Amagasaki Central Hospital Hyogo Japan Ohara Internal Medicine and Cardiology Clinic Hyogo Japan Departments of Pathology Amagasaki Central Hospital Hyogo Japan Departments of Surgery Amagasaki Central Hospital Hyogo Japan

We could observe the endoluminal stent graft (SG) following thoracic endovascular aneurysm repair (TEVAR) by a coronary artery angioscope and establish intravascular visualization of SG. The patient was a 70-year-old woman with the distal aortic arch aneurysm and the descending aortic aneurysm, and debranching TEVAR were performed. After 12 months follow up, urgent hospitalization was required for chest pain, and cardiac catheter examination with a coronary artery angioscope was performed. The endoluminal SG was observed. The observation in angioscope which is a video image is better than CT that is a still image, and observation in blood vessel or SG is possible. It may be possible to observe the endoluminal SG, allowing potential investigation of an endoleak, or the covering status of the SG with the native aortic vessel wall, or the state of intimal membrane formation in the endoluminal graft.

关键词： Intravascular Visualization Angioscopy Endoluminal Stent Graft Endovascular Aneurysm Repair

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Greenhouse gas reduction of co-benefit-type wastewater treatment system for fish-processing industry: A real-scale case study in Indonesia

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Water Science and Engineering 2023年第3期16卷 271-279页

作者： Yoshiteru Hamatani takahiro Watari Masashi Hatamoto Takashi yamaguchi Tjandra Setiadi Toshihiko Konda Nihon Suido Consultants Co. LtdTokyo 163-1122Japan Department of Civil and Environmental Engineering Nagaoka University of TechnologyNiigata 940-2188Japan Department of Chemical Engineering Hanoi University of Science and TechnologyHanoi 11615Vietnam Department of Science of Technology Innovation Nagaoka University of TechnologyNiigata 940-2188Japan Department of Chemical Engineering Institut Teknologi BandungJl.Ganesa 10Bandung 40132Indonesia

This study examined the application of co-benefit-type wastewater treatment technology in the fish-processing industry. Given that there was a dearth of information on fish-processing industrial wastewater in Indonesia, site surveys were conducted. For the entire fish-processing industry throughout the country, the dissemination rate of wastewater treatment facilities was less than 50%. Using a co-benefit approach, a real-scale swim-bed technology (SBT) and a system combining an anaerobic baffled reactor (ABR) with SBT (ABR–SBT) were installed in a fishmeal processing factory in Bali, Indonesia, and the wastewater system process performance was evaluated. In a business-as-usual scenario, the estimated chemical oxygen demand load and greenhouse gas (GHG) emissions from wastewater from the Indonesian fish-processing industry were 33 000 tons per year and 220 000 tons of equivalent CO_(2) per year, respectively. On the other hand, the GHG emissions in the co-benefit scenarios of the SBT system and ABR–SBT system were 98 149 and 26 720 tons per year, respectively. Therefore, introducing co-benefit-type wastewater treatment to Indonesia’s fish-processing industry would significantly reduce pollution loads and GHG emissions.

关键词： Indonesian fish-processing industry Co-benefit wastewater treatment Greenhouse gas emission mitigation Anaerobic baffled reactor Swim-bed technology

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A dwarf bamboo(Pleioblastus chino) and winter browsing by Japanese hare(Lepus brachyurus) combine to limit establishment of transplanted native tree seedlings in an abandoned agricultural field

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Journal of Forestry Research 2016年第6期27卷 1287-1294页

作者： Yoshinori Tokuoka Kentaro Ohigashi Koji Watanabe Hiroshi yamaguchi takahiro Ara Nobukazu Nakagoshi Biodiversity Division National Institute for Agro-Environmental Sciences (NIAES) Ecosystem Informatics Division NIAES Experimental Farm Management Division NIAES Graduate School for International Development and Cooperation Hiroshima University

Natural forest recovery on abandoned farmland is hindered by a variety of factors and active restoration plays an important role when quick afforestation is desired. We investigated seedling survival of four transplanted native tree species （Quercus myrsinifolia, Quercus serrata, Aphananthe aspera, and Rhus sylvestris） by experimentally manipulating the vegetation cover, which was mainly dominated by dwarf bamboo （Pleioblastus chino）, and herbivore access to the planting sites on farmland that had been abandoned for 15 years at the start of the study. Few transplanted seedlings of any species survived under intact vegetation cover, irrespective of herbivore presence. In gaps in the vegetation cover, winter browsing by Japanese hare （Lepus brachyurus） damaged all species. However, lower browsing frequency and higher resprouting ability after grazing of the seedlings enabled both Quercus species to survive better than the other species. These results indicate that dwarf bamboo and the hare jointly limit the establishment of native trees in old fields. If active afforestation by transplanting seedlings at sites dominated by dwarf bamboo is planned, a combination of vegetation removal, selection of suitable species, and temporary seedling protection will be most effective.

关键词： Farmland abandonment Microsite limitation Monodominant dwarf bamboo Resprouting Seedling herbivory

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