The TLR Expression Pattern on Monocyte-Derived Macrophages for Lipopolysaccharid Stimulation of Calves

作     者：GUO Yi-jie ZHAO Guo-Qi HUO Yong-jiu Sachi Tanaka Hisashi Aso Takahiro Yamaguchi 

作者机构：Laboratory of Animal Nutrition Graduate School of Animal Science and Technology Yangzhou University Yangzhou 225009 P.R. China Laboratory of Functional Morphology Graduate School of Agricultural Science Tohoku University 1-1 Tsutsumidori-Amamiyamachi Aobaku 981-8555 Sendai Japan 

出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)

年 卷 期：2009年第8卷第7期

页      面：864-871页

核心收录：

学科分类：09[农学] 

基　　金：supported by the National Natural Science Foundation of China(30871800, 30471259) the National Key Technology R&D Program of China (2006BAD04A03-07) 

主　　题：TLR bovine macrophage LPS 

摘      要：In this paper, toll-like receptor expression pattern in monocytes-derived macrophages by lipopolysaccharid （LPS） stimulation was examined. Jugular venous blood samples from 4 Japanese calves were obtained and the peripheral blood mononuclear cells （PBMC） were isolated. The PBMC were cultured for 7 d so as to collect monocytes-derived macrophages in Repcell. The PBMC were stimulated by LPS for 24 h and the mRNA expression pattern of TLR and cytokines in monocytes-derived macrophages （Mod-Mφ） was analyzed. Results showed that LPS stimulation of Mod-Mφ could increase the mRNA levels of the genes of TNF-α, IL-6, and IL-8. In addition, the mRNA levels of the genes of TNF-α and IL-6 in the group of LPS stimulation were most significantly （P 〈 0.01） higher than those in control group and the mRNA levels of TLR1, 3, 5, 8, and 10 were significantly （P 〈 0.05） decreased after LPS stimulation. There was no difference in the mRNA expressions of TLR2, 4, 6, and 7 between the groups of the control and LPS stimulation. Besides, expression of TLR9 was not found. It suggested that monocytes-derived macrophages could respond to LPS and they might take an important role in the innate immunity. The important function of the cells might contribute to better disease treatment.