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Risk of infection with arboviruses in a healthy population in Pakistan based on seroprevalence

VIROLOGICA SINICA 2024年第3期39卷 369-377页

作者： Chen, Shengyao Saqib, Muhammad Khan, Hafiz Sajid Bai, Yuan Ashfaq, Usman Ali Mansoor, Muhammad Khalid Moming, Abulimti Liu, Jing Zhou, Min Niazi, Saifullah Khan Wu, Qiaoli Sial, Awais-Ur-Rahman Tang, Shuang Sarfraz, Muhammad Hassan Javed, Aneela Hayat, Sumreen Khurshid, Mohsin Khan, Iahtasham Athar, Muhammad Ammar taj, zeeshan Zhang, Bo Deng, Fei Zohaib, Ali Shen, Shu Chinese Acad Sci Key Lab Virol Wuhan 430071 Peoples R China Chinese Acad Sci Wuhan Inst Virol Natl Virus Resource Ctr Wuhan 430071 Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China Hubei Jiangxia Lab Wuhan 430200 Peoples R China Univ Agr Faisalabad Fac Vet Sci Faisalabad 38040 Pakistan Childrens Hosp Faisalabad Faisalabad 38000 Pakistan Govt Coll Univ Faisalabad Dept Bioinformat & Biotechnol Faisalabad 38000 Pakistan Islamia Univ Bahawalpur Fac Vet & Anim Sci Dept Microbiol Bahawalpur 63100 Pakistan Chinese Acad Sci Wuhan Inst Virol Ctr Biosafety Mega Sci Key Lab Special Pathogens & Biosafety Wuhan 430071 Hubei Peoples R China Armed Forces Inst Pathol AFIP Rawalpindi 46000 Pakistan Pir Mehr Ali Shah Arid Agr Univ Fac Vet & Anim Sci Dept Clin Studies Rawalpindi 46300 Pakistan Govt Coll Univ Faisalabad Inst Microbiol Faisalabad 38000 Pakistan Natl Univ Sci & Technol Atta Ur Rahman Sch Appl Biosci Islamabad 44000 Pakistan Univ Vet & Anim Sci Dept Clin Sci Lahore Subcampus Jhang 35200 Pakistan Natl Med Ctr Dept Mol Pathol DHA Phase 1 Karachi 75500 Pakistan

Infectious diseases caused by arboviruses are a public health concern in Pakistan. However, studies on data prevalence and threats posed by arboviruses are limited. This study investigated the seroprevalence of arboviruses in a healthy population in Pakistan, including severe fever with thrombocytopenia syndrome virus (SFTSV), Crimean-Congo hemorrhagic fever virus (CCHFV), Tamdy virus (TAMV), and Karshi virus (KSIV) based on a newly established luciferase immunoprecipitation system (LIPS) assays, and Zika virus (ZIKV) by enzyme-linked immunosorbent assays (ELISA). Neutralizing activities against these arboviruses were further examined from the antibody positive samples. The results showed that the seroprevalence of SFTSV, CCHFV, TAMV, KSIV, and ZIKV was 17.37%, 7.58%, 4.41%, 1.10%, and 6.48%, respectively, and neutralizing to SFTSV (1.79%), CCHFV (2.62%), and ZIKV (0.69%) were identified, as well as to the SFTSV-related Guertu virus (GTV, 0.83%). Risk factors associated with the incidence of exposure and levels of antibody response were analyzed. Moreover, co-exposure to different arboviruses was demonstrated, as thirty-seven individuals were having antibodies against multiple viruses and thirteen showed neutralizing activity. Males, individuals aged <= 40 years, and outdoor workers had a high risk of exposure to arboviruses. All these results reveal the substantial risks of infection with arboviruses in Pakistan, and indicate the threat from co-exposure to multiple arboviruses. The findings raise the need for further epidemiologic investigation in expanded regions and populations and the necessity to improve health surveillance in Pakistan.

关键词： Arbovirus Pakistan Seroprevalence Tick -borne virus Mosquito -borne virus

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Influence of Brownian Motion, Thermophoresis and Magnetic Effects on a Fluid Containing Nanoparticles Flowing over a Stretchable Cylinder

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Fluid Dynamics & Materials Processing 2024年第3期20卷 525-536页

作者： Aaqib Majeed Ahmad zeeshan Department of Mathematics The University of FaisalabadFaisalabad38000Pakistan Department of Mathematics and Statistics FBASInternational Islamic University IslamabadIslamabad44000Pakistan

The influence of Brownian motion and thermophoresis on a fluid containing nanoparticles flowing over a stretchable cylinder is *** classical Navier-Stokes equations are considered in a porous *** addition,the Lorentz force is taken into *** controlling coupled nonlinear partial differential equations are transformed into a system of first order ordinary differential equations by means of a similarity *** resulting system of equations is solved by employing a shooting approach properly implemented in *** evolution of the boundary layer and the growing velocity is shown graphically together with the related profiles of concentration and *** magnetic field has a different influence(in terms of trends)on velocity and concentration.

关键词： Mixed convection Brownian motion heat transfer porous surface velocity slip

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Seasonal variations in dissolved organic matter concentration and composition in an outdoor system for bank filtration simulation

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Journal of Environmental Sciences 2024年第1期135卷 252-261页

作者： Muhammad zeeshan Omamah Ali Shamas Tabraiz Aki Sebastian Ruhl German Environment Agency Section II 3.3Schichauweg 5812307BerlinGermany Technische Universitat Berlin Water TreatmentKF4Str.des 17.Juni 13510623BerlinGermany Natural and Applied Sciences Section School of Psychology and Life SciencesCanterbury Christ Church UniversityCanterburyCT11QUUK

Dissolved organic matter(DOM)in surface waters can vary markedly in character depending on seasonal variations such as rainfall intensity,UV radiations and *** in DOM as well as temperature and rainfall intensity over the year can affect the biochemical processes occurring in bank filtration(BF).Identification and characterization of DOM in the surface water could help to optimize the water treatment and provide stable and safe drinking *** study investigated year-long variations of DOM concentrations and compositions in a surface water of a circulated outdoor pond(research facility)connected to a BF *** was dominated by humic substances and a changing pattern of DOM in surface water was observed throughout the year.A significant increase of DOM(~38%)in surface water was noted in August compared to *** fluorescent DOM showed that DOM in summer was enriched with the degradable fraction whilst non-degradable fraction was dominated in winter.A constant(1.7±0.1 mg/L)effluent DOM was recirculated in the system throughout the *** removal through BF varied between 4%to 39%and was achieved within a few meters after infiltration and significantly correlated with influent DOM concentration(R^(2)=0.82,p0.05)change in the removal of DOM was observed in two subsurface layers(upper and lower).This study highlights the presence of a constant non-degradable DOM in the bank filtrate,which was not affected by temperature,redox conditions and UV radiations.

关键词： Bank filtration Dissolved organic matter Dissolved oxygen Fluorescence spectroscopy Excitation-emission matrix

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Current epidemiologic features and health dynamics of ESBL-producing Escherichia coli in China

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Biosafety and Health 2024年第1期6卷 40-49页

作者： Sayed Salman zeeshan Umar Yonghong Xiao Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases State Key Laboratory for Diagnosis and Treatment of Infectious Diseasesthe First Afiliated Hospital College of MedicineZhejiang UniversityHangzhou 310000China Marshall Laboratory of Biomedical Engineering School of MedicineShenzhen UniversityShenzhen 510000China

Extended‐spectrumβ‐lactamase(ESBL)‐producing Escherichia coli(***)are widespread in China,with occurrences documented in humans,animals,and the *** dissemination of ESBL‐producing *** is likely facilitated by the widespread use of antibiotics in human and animal agriculture,the presence of antibiotic‐resistant bacteria(ARBs)in animal feces,and close human‐animal ***,particularly those belonging to incompatibility(Inc)group,such as IncF,IncI,and IncH families,play a vital role in facilitating the horizontal gene transfer of ESBL genes across various sectors,from humans to animals and the *** 26 and IS 1 elements also significantly influences the mobilization and evolution of antibiotic‐resistance genes(ARGs),contributing to the spread of ESBL‐producing ***‐M-14,blaCTX-15,and blaCTX‐M-55 are prevalent in ESBL‐producing *** across the three domains and are often found in con-junction with other *** these challenges,it is imperative to take proactive measures to prevent the further spread of *** includes the judicious and responsible use of antibiotics and efforts to mini-mize contact with animal ***‐specific strategies should be developed to effectively educate and engage relevant personnel in tackling this multifaceted *** efforts are vital to combat the dissem-ination of ESBL‐producing *** and preserve public health.

关键词： Escherichia coli Extended‐spectrumβ‐lactamases CTX‐M

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Ionotropic receptors mediate olfactory learning and memory in Drosophila

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Insect Science 2024年第4期31卷 1249-1269页

作者： Md zeeshan Ali Anushree Aarif Ahsan Mohammad Shamsul Ola Rizwanul Haque Jawaid Ahsan Department of Biotechnology Central University of South BiharGayaBiharIndia Department of Radiation Oncology University of MichiganAnn ArborMichiganUSA Department of Biochemistry College of ScienceKing Saud UniversityRiyadhSaudi Arabia

Phenylacetaldehyde(PAH),an aromatic compound,is present in a diverse range of fruits including overripe bananas and prickly pear cactus,the two major host fruits for Drosophila *** acts as a potent ligand for the ionotropic receptor 84a(IR84a)in the adult fruit fly and it is detected by the IR84a/IR8a heterotetrameric *** role in the male courtship behavior through IR84a as an environmental aphrodisiac is of additional *** ***,two distinct kinds of olfactory receptors,that is,odorant receptors(ORs)and ionotropic receptors(IRs),perceive the odorant *** display unique structural,molecular,and functional characteristics in addition to having different evolutionary ***,olfactory cues detected by the ORs such as ethyl acetate,1-butanol,isoamyl acetate,1-octanol,4-methylcyclohexanol,*** as aliphatic esters and alcohols have been employed in olfactory classical conditioning using fruit *** underlines the participation of OR-activated olfactory pathways in learning and memory *** study elucidates that likewise ethyl acetate(EA)(an OR-responsive odorant),PAH(an IR-responsive aromatic compound)too can form learning and memory when associated with an appetitive gustatory *** association of PAH with sucrose(PAH/SUC)led to learning and formation of the long-term memory(LTM).Additionally,the Orco^(1),Ir84a^(MI00501),and Ir8a^(1)mutant flies were used to confirm the exclusive participation of the IR84a/IR8a complex in PAH/SUC olfactory associative *** results highlight the involvement of IRs via an IR-activated pathway in facilitating robust olfactory behavior.

关键词： appetitive conditioning Drosophila melanogaster ethyl acetate ionotropic receptors learning and memory phenylacetaldehyde

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Depositional and Bio-Sequence Stratigraphic Framework of Late Cretaceous Kawagarh Formation,Kala-Chitta Range,Pakistan:Equivalent of Gurpi Formation(Iran)

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Journal of Earth Science 2024年第2期35卷 332-348页

作者： Muhammad Awais Bilal Wadood Muhammad Ishaq Muhammad Bilal zeeshan Zafar Nasar Khan Aminullah Khan Department of Geology University of SwabiAnbar-SwabiKhyber PakhtunkhwaSwabi23561Pakistan Department of Earth Environmental and Resources SciencesUniversity of Naples FedericoⅡNaples80126Italy State Key Laboratory of Continental Dynamics Department of GeologyNorthwest UniversityXi’an710069China National Centre of Excellence in Geology University of PeshawarPeshawarKhyber Pakhtunkhwa25130Pakistan Department of Geological Engineering School of Earth Sciences and EngineeringXi’an Shiyou UniversityXi’an610500China Department of Petroleum Geology and Engineering School of Earth ResourcesChina University of GeosciencesWuhan430074China Department of Geology University of MalakandDir Lower18800ChakdaraPakistan Department of Earth and Environmental Sciences KU LeuvenLeuven3001Belgium

The present study is focused on evaluation of deep marine pelagic sediments of Late Cretaceous Kawagarh Formation of Kala-Chitta Range in the context of microfacies analysis,paleoenvironmental interpretation,planktonic foraminiferal biostratigraphy,sequence stratigraphy and diversification of species.A total of thirty three rock samples were collected from the measured *** microfacies are interpreted,namely planktonic foraminifera wackestone,planktonic foraminifera mudstone and sandy mudstone indicating low energy depositional environment i.e.,outer *** biostratigraphic studies show plentiful planktonic foraminifera species of Globotruncana,Heterohelix and ***,no association of benthic or siliceous organisms was *** the basis of available species assemblage,a single local planktonic foraminifera biozone i.e.,Globotruncana-Heterohelix-Globotruncanita Assemblage Biozone is *** biozone information is combined with published literature and Lower Santonian to Middle Maastrichtian age has been assigned to the Kawagarh *** trend of species occurrences evinces that species number decreases over time with pulsated rise in the Kawagarh *** Kawagarh Formation carbonates show an overall Transgressive Systems Tract(TST).The Kawagarh Formation of Pakistan evinces analogous characteristics to that of the Late Cretaceous Gurpi Formation of Iran based on the geologic age,outcrop lithology,microfacies,planktonic foraminiferal assemblages,depositional setting and sequence stratigraphy.

关键词： depositional fabric biostratigraphy sequence stratigraphy Late Cretaceous Kawagarh Formation Pakistan Gurpi Formation Iran

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Countrywide Survey for MERS-Coronavirus Antibodies in Dromedaries and Humans in Pakistan

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Virologica Sinica 2018年第5期33卷 410-417页

作者： Ali Zohaib Muhammad Saqib Muhammad Ammar Athar Jing Chen Awais-ur-Rahman Sial Saeed Khan zeeshan taj Halima Sadia Usman Tahir Muhammad Haleem Tayyab Muhammad Asif Qureshi Muhammad Khalid Mansoor Muhammad Ahsan Naeem Bing-Jie Hu Bilal Ahmed Khan Ikram Din Ujjan Bei Li Wei Zhang Yun Luo Yan Zhu Cecilia Waruhiu Iahtasham Khan Xing-Lou Yang Muhammad Sohail Sajid Victor Max Corman Bing Yan Zheng-Li Shi CAS Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Ctunese Academy of Sciences Beijing 100049China Faculty of Veterinary Medicine University of AgricultureFaisalabad 38040Pakistan Laboratory of Cancer Biomarkers and Liquid Biopsy College of PharmacyHenan UniversityHenan 475004China Department of Clinical Studies Faculty of Veterinary and Animal SciencesPMAS-Arid Agriculture UniversityRawalpindi 46000Pakistan Dow University of Health Science Pakistan Karachi 74200 Department of Microbiology Government College University FaisalabadFaisalabad 38000Pakistan Livestock and Dairy Development Department(L&DD) Lahore 54000Pakistan College of Veterinary and Animal Sciences The Islamia University of BahawalpurBahawalpur 63100Pakistan State Key Laboratory of Agricultural Mlcroblology Huazhong Agricultural UniversityWuhan 430070China Liaqat University of Medical and Health Science Pakistan Jamshoro 76090 Section of Epidemiology and Public Health Department of Chnical SciencesUniversity of Veterinary and Animal SciencesLahore subcampusJhang 35200Pakistan Institute of Virology Chadte-Universitatsmedizin BerlinCorporate member of Free UniversityHumboldt-University and Berlin Institute of HealthBerlin 10117Germany German Center for Infection Research(DZIF) Berlin 10117Germany

Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a zoonotic pathogen capable of causing severe respiratory disease in humans. Although dromedary camels are considered as a major reservoir host, the MERS-CoV infection dynamics in camels are not fully understood. Through surveillance in Pakistan, nasal (n = 776) and serum (n = 1050)samples were collected from camels between November 2015 and February 2018. Samples were collected from animal markets, free-roaming herds and abattoirs. An in-house ELISA was developed to detect IgG against MERS-CoV. A total of 794 camels were found seropositive for MERS-CoV. Prevalence increased with the age and the highest seroprevalence was recorded in camels aged [ 10 years (81.37%) followed by those aged 3.1–10 years (78.65%) and B 3 years (58.19%).Higher prevalence was observed in female (78.13%) as compared to male (70.70%). Of the camel nasal swabs, 22 were found to be positive by RT-qPCR though with high Ct values. Moreover, 2,409 human serum samples were also collected from four provinces of Pakistan during 2016–2017. Among the sampled population, 840 humans were camel *** we found a high rate of MERS-CoV antibody positive dromedaries (75.62%) in Pakistan, no neutralizing antibodies were detected in humans with and without contact to camels.

关键词： Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Camel -Human Pakistan

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MAX-DOAS and in-situ measurements of aerosols and trace gases over Dongying,China:Insight into ozone formation sensitivity based on secondary HCHO

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Journal of Environmental Sciences 2024年第1期135卷 656-668页

作者： Xiaojun Zheng zeeshan Javed Cheng Liu Aimon Tanvir Osama Sandhu Haoran Liu Xiangguang Ji Chengzhi Xing Hua Lin Daolin Du Institute of Environment and Ecology School of the Environment and Safety EngineeringJiangsu UniversityZhenjiang 212013China Department of Precision Machinery and Precision Instrumentation University of Science and Technology of ChinaHefei 230026China Key Laboratory of Environmental Optics&Technology Anhui Institute of Optics and Fine MechanicsHefei Institutes of Physical ScienceChinese Academy of SciencesHefei 230031China Center for Excellence in Regional Atmospheric Environment Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education Institutes University of Science and Technology of ChinaHefei 230026China Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention(LAP3) Department of Environmental Science and EngineeringFudan UniversityShanghai 200433China National Agromet Center Pakistan Meteorological DepartmentIslamabad 44000Pakistan Institute of Physical Science and Information Technology Anhui UniversityHefei 230601China Information Materials and Intelligent Sensing Laboratory of Anhui Province Anhui UniversityHefei 230601China School of Environmental Science and Optoelectronic Technology University of Science and Technology of ChinaHefei 230026China

This study presents a comprehensive overview of the atmospheric pollutants including Sulfur dioxide(SO_(2)),Nitrogen dioxide(NO_(2)),Formaldehyde(HCHO),Particulate Matter PM;PM_(10):diameter≤10μm,and PM_(2.5):diameter≤2.5μm,and Ozone(O_(3)),over Dongying(Shandong Province)from March-April 2018 and September-October 2019 by employing ground-based Multiple Axis Differential Optical Absorption Spectroscopy(MAX-DOAS)observations along with the in-situ measurements attained by the national air quality monitoring *** concentrations of SO_(2)and NO_(2)were under the acceptable level,while both PM_(2.5),and PM_(10)were higher than the safe levels as prescribed by national and international air quality *** results depict that 21%of the total observation days were found to be complex polluted days(PM_(2.5)>35μg/m^(3) and O_(3)>160μg/m^(3)).The secondary HCHO was used for accurate analysis of O_(3)sensitivity.A difference of 11.40%and 10%during March-April 2018 and September-October 2019 respectively in O_(3)sensitivity was found between HCHO_(total)/NO_(2)and HCHO_(sec)/NO_(2).The results indicate that primary HCHO have significant contribution in HCHO.O_(3)formation predominantly remained to be in VOC-limited and transitional regime during March-April 2018 and September-October 2019 in *** results imply that concurrent control of both NO_(x) and VOCs would benefit in ozone ***,the criteria pollutants(PM,SO_(2),and NO_(2))depicted strong correlations with each other except for O_(3)for which weak correlation coefficient was obtained with all the *** study will prove to be baseline for designing of air pollution control strategies.

关键词： MAX-DOAS Trace gases Ozone sensitivity Secondary HCHO Complex Pollution

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Source parameters and aftershock pattern of the October 7,2021,M5.9 Harnai earthquake,Pakistan

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Earthquake Science 2024年第4期37卷 304-323页

作者： Mohammad Tahir zeeshan Ahmad Sadia Sabahat Muhammad Naveed Mushtaq Talat Iqbal Muhammad Ali Shah Alam Aftab Centre for Earthquake Studies National Centre for PhysicsIslamabad 44000Pakistan

On October 7,2021,a magnitude 5.9 earthquake struck the Harnai(Baluchistan)region of Pakistan,causing several fatalities and injuries within the epicentral ***-order tectonic deformation in this region is caused by the convergence of the Indian Plate with respect to the Eurasian *** Katwaz Block hinders the motion of the Indian Plate,resulting in the formation of strike-slip *** this study,the P-wave first-motion polarity technique was used to determine the mainshock faulting *** scanning of the polarity solutions was applied to determine the most suitable focal mechanism solution among the available solutions generated by the FOCMEC(focal mechanism)*** nodal planes correspond to different faulting styles(i.e.,thrust and strike-slip faulting).A nodal plane oriented in the NW-SE direction corresponded to a strike-slip mechanism,which was considered to be the fault ***,this earthquake was associated with the Harnai-Karahi strike-slip fault zone owing to the fault strike and direction of *** apparent stress drop,fault length,and moment magnitude of the Harnai earthquake were 35.4 bar,6.1 km,and 5.9,respectively.A lower b-value for the Gutenberg-Richter law was observed prior to the ***α-than b-values(α>b)indicate that this earthquake was governed by large events as opposed to small-magnitude *** Harnai sequence had a decay exponent close to unity,lasted for 145 days,and produced few *** study will help the future hazard mitigation in the region.

关键词： focal mechanism solution selection of fault plane FPFIT stress inversion oblique slip mechanism

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The Condyloma Acuminatum’s Recurrence Can Be Reduced by Lesional Auto-Transplantation

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Journal of Cosmetics, Dermatological Sciences and Applications 2024年第3期14卷 242-252页

作者： Epipode Ntawuyamara Bashir zeeshan Yuan Liang Yanhua Liang Department of Dermatology Cosmetology and Venereology Shenzhen Hospital of Southern Medical University Shenzhen China Department of Dermatology and Venereology Kamenge Teaching Hospital of University of Burundi Bujumbura Burundi Shenzhen (Nanshan) Concord College of Sino-Canada Shenzhen China

Background : Condyloma acuminatum (CA), also known as a genital wart (GW), is a sexually transmitted disease caused by human papillomavirus (HPV). HPV lesions, recurrence tendency, and risk of malignant formation are primarily dependent on the person’s immunity level. GW recurrence is a major challenge in CA treatment. The aim of this study was to explore how lesional auto-transplantation (LT) can be used to treat CA and decrease its recurrence. Methods : We treated CA through the preparation and implantation of tissue from nine CA patients in our dermatology clinic. Transplantation of small pieces of HPV lesions to the subcutaneous fat of the inguinal region was carried out with the help of a simple surgical method under local anesthesia. Patients were followed up for six to eight months. We searched PubMed and the Web of Science for treatment options for CA to compare our treatment method recurrence rate with existing treatment options. Results : During three months of follow-up, seven patients experienced no recurrence of condyloma lesions, while two patients experienced recurrence of small lesions of condyloma acuminatum. The recurrence rate of CA once treated by auto-transplantation was relatively low compared to other CA treatment options. Conclusions : Transplantation of HPV lesions to the inguinal area reduces the recurrence rate in sexually active individuals. The 2/9 recurrence after implantation could be due to poor hygiene, sexual relationships or immune factors.

关键词： Auto-Transplantation Condyloma Acuminatum Human Papillomavirus Recurrence

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