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The Crop Journal 2024年第3期12卷 866-879页

作者： Haozhe Tan Binghui Tang Mengling Sun Qiulu Yin Yizan Ma Jianying Li Pengcheng Wang Zhonghua Li Guannan Zhao Maojun Wang Xianlong Zhang Chunyuan You lili tu National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070HubeiChina Cotton Research Institute Shihezi Academy of Agriculture ScienceShihezi 832000XinjiangChina

Cotton fiber is one of the main raw materials for the textile *** recent years,many cotton fiber quality QTL have been identified,but few were applied in *** this study,a genome wide association study(GWAS)of fiber-quality traits in 265 upland cotton breeding intermediate lines(GhBreeding),combined with genome-wide selective sweep analysis(GSSA)and genomic selection(GS),revealed 25 *** of these QTL were ignored by only using *** CRISPR/Cas9 mutants of GhMYB_D13 had shorter fiber,which indicates the credibility of QTL to a certain *** these QTL were verified in other cotton natural populations,5 stable QTL were found having broad potential for application in ***,among these 5 stable QTL,superior genotypes of 4 showed an enrichment in most improved new varieties widely cultivated *** findings provide insights for how to identify more QTL through combined multiple genomic analysis to apply in breeding.

关键词： Cotton fiber quality breeding GWAS Genome-wide selective sweep analysis Genomic selection InDel markers

The laccase gene Gh Lac1 modulates fiber initiation and elongation by coordinating jasmonic acid and flavonoid metabolism

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The Crop Journal 2020年第4期8卷 522-533页

作者： Qin Hu Shenghua Xiao Qianqian Guan lili tu Feng Sheng Xuezhu Du Xianlong Zhang State Key Laboratory of Biocatalysis and Enzyme Engineering School of Life SciencesHubei UniversityWuhan 430000HubeiChina National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430000HubeiChina

Cotton fibers are single cells originating in the epidermis of cotton ovules,and serve as the largest natural fiber source for the textile *** theory,all epidermal cells have the potential to develop into fibers,but only 15%–25%of epidermis cells develop into commercially viable lint *** previously showed that Gh Lac1 participates in cotton defense against biotic *** we report that Gh Lac1 also has a role in cotton fiber *** Lac1 RNAi lines in cotton showed increased differentiation of fiber initials from epidermis and shortened fiber length,resulting in unchanged lint *** of Gh Lac1 expression led to constitutively hyperaccumulated jasmonic acid(JA)and flavonoids in ovules and fiber *** vitro ovule culture experiments confirmed the distinct roles of JA and flavonoids in fiber initiation and elongation,and showed that fiber development is spatially regulated by these chemicals:the increased fiber initiation in Gh Lac1 RNAi lines is caused by hyperaccumulated JA and rutin content during the fiber initiation stage and shortened fiber length is caused by constitutively increased JA and naringenin content during the fiber elongation stage.

关键词： Cotton GhLac1 Fiber development Jasmonic acid Flavonoids

Repression of microRNA 160 results in retarded seed integument growth and smaller final seed size in cotton

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The Crop Journal 2020年第4期8卷 602-612页

作者： Nian Liu Shuchang Wu Zhonghua Li Anam Qadir Khan Haiyan Hu Xianlong Zhang lili tu National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture and Rural AffairsOil Crops Research Institute of the Chinese Academy of Agricultural SciencesWuhan 430062HubeiChina

microRNA 160(miR160),targeting auxin response factors(ARFs),plays many roles in plant *** investigated the role of the mi R160/ARF axis in regulation of cotton seed *** mi R160 activity,specifically in the seed coat,led to smaller seeds and less fiber production owing to attenuated growth of the maternal *** electron microscopy and histology showed that expansion of cells in the integument was retarded in mi R160-suppressed *** Gh ARF genes were targeted by mi R160 and were upregulated in mi R160-suppressed lines,indicating that a mi R160/ARF axis is present in *** genes(Ghir_A05 G003740,Scaffold1878 G000010,Ghir_D09 G024980,Ghir_A11 G010730,and Ghir_A05 G041590),associated with reduced seed development were downregulated in mi R160-suppressed *** results suggest that the mi R160/ARF axis controls maternal integument growth to influence seed size by directly or indirectly regulating seed development-associated genes.

关键词： size cotton maternal

Studies of new EST-SSRs derived from Gossypium barbadense

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Chinese Science Bulletin 2007年第18期52卷 2522-2531页

作者： ZHANG YanXin LIN ZhongXu LI Wu tu lili NIE YiChun ZHANG XianLong National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 China

Existing cotton EST-SSR markers are mostly derived from Gossypium arboreum and Gossypium hir-sutum, but EST-SSR markers from Gossypium barbadense are scarce. One hundred and nineteen EST-SSRs were developed based on 98 unique ESTs from a cDNA library constructed in our laboratory using developing fibers from G. barbadense cv. Pima3-79. Among the SSRs, trinucleotide AAG appeared at a high frequency of 11.76%. 36 accessions (consisting of 13 diploids of the A genome, 11 diploids of the D genome and 12 allotetraploids of the AD genome) were employed to test new EST-SSRs. 76 EST-SSRs were successfully amplified, and 313 polymorphic fragments were yielded, with an average of 4.11 fragments per primer pair. The PIC ranged from 0.17 to 0.95 with an average of 0.53. Based on Jaccard’s genetic similarity coefficient, these 36 accessions were clustered into three groups. 21 EST-SSRs exhibited polymorphisms in BC1 population ((Emian22 × Pima3-79) × Emian22), 24 polymor- phic loci were generated, while 22 of the 24 polymorphic loci were integrated with our interspecific BC1 backbone genetic linkage map, and anchored in 12 chromosomes. This study effectively proved that EST-SSRs from G. barbadense are valuable for genetic diversity analysis and genetic mapping.

关键词：棉花基因表达基因序列分析遗传多样性基因图谱

Comparative genomic analyses reveal cis-regulatory divergence after polyploidization in cotton

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The Crop Journal 2022年第6期10卷 1545-1556页

作者： Jiaqi You Min Lin Zhenping Liu Liuling Pei Yuexuan Long lili tu Xianlong Zhang Maojun Wang National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070HubeiChina School of Pharmaceutical Sciences South-Central University for NationalitiesWuhan 430074HubeiChina

Polyploidization has long been recognized as a driver for the evolutionary formation of superior plant traits coupled with gene expression ***,knowledge of the effect of regulatory variation on expression changes following polyploidization remains *** this study,we characterized transcriptional regulatory divergence by comparing tetraploid cotton with its putative diploid *** identified 144,827,99,609,and 219,379 Tn5 transposase-hypersensitive sites(THSs)in Gossypium arboreum,***,and ***,respectively,and found that the conservation of promoter THSs was associated with coordination of orthologous genes *** observation was consistent with analysis of transcription-factor binding sites(TFBS)for 262 known motifs:genes with higher TFBS conservation scores(CS)showed less change than those genes with lower TFBS CS in expression *** influenced by genomic variation were involved in the novel regulation networks between transcriptional factors and target genes in tetraploid *** describe an example showing that the turnover of TFBS was linked to expression pattern divergence of genes involved in fiber development(fiber-related genes).Our findings reveal the regulatory divergence of the transcriptional network in cotton after polyploidization and characterizes the regulatory relationships of genes contributing to desirable traits.

关键词： Cotton Polyploidization Transcriptional regulation Fiber development

Analysis of genes differentially expressed during initial cellular dedifferentiation in cotton

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Chinese Science Bulletin 2008年第23期53卷 3666-3676页

作者： ZHU HuaGuo tu lili JIN ShuangXia XU Li TAN JiaFu DENG FengLin ZHANG XianLong National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 China

The early phase of phytohormone induction is a vital stage of somatic embryogenesis. This phase includes a key process for acquiring cellular totipotency through cellular dedifferentiation. To unravel the molecular mechanism of cellular dedifferentiation in cotton, we constructed a cDNA library using the suppression subtractive hybridization method. A total of 286 differential cDNA clones were sequenced and identified. Among these clones, 112 unique ESTs were significantly up-regulated during the early phase of phytohormone induction, and 40.2% of the ESTs were first identified. GST was highly expressed from 6 to 24 h after induction with phytohormone treatment. PRPs were predominantly expressed and exhibited distinct expression patterns in different treatments, suggesting that they are closely related to cellular dedifferentiation in cotton. Putative GhSAMS, GhSAMDC, GhSAHH and GhACO3 involvement in SAM metabolism was identified in this library. The analysis of qRT-PCR showed that two remarkable increased expressions of the four SAM-related genes happened during the early phase of phytohormone induction, and that a highly positive correlation existed between GhSAMS and GhSAHH. The highest expression level of GhSAMS might be associated with its reentry into the cell cycle. The histological observations further showed that some cells accomplished cellular dedifferentiation and division within 72 h in 2,4-D treatment, and that cellular dedifferentiation might be regulated through two alterations in SAM-dependent transmethylation activity in cotton. In addition, the expression patterns of differential genes in different treatments disclosed the complicated interaction between 2, 4-D and kinetin.

关键词：细胞去分化棉花脯氨酸蛋白质转甲基作用

CPAFFC Delegation Visits Nepal and Bangladesh

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Voice of Friendship 2009年第4期 22-24页

作者： tu lili

At the invitation of the Nepal China Executives Council (NCEC) and the Bangladesh-China People’s Friendship Association (BCPFA), a CPAFFC delegation led by

关键词： Bangladesh invitation delegation visited minister

CIFA Delegation Led by Its President Jiang Zhenghua Visits India

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Voice of Friendship 2009年第2期 22-24页

作者： tu lili

At the invitation of the India Council of Cultural Relations (ICCR), Jiang Zhenghua, president of the China-India Friendship Association (CIFA) and former vice chairman of the Standing Committee of the

关键词： India invitation delegation chairman president coo

Delegation of Association for Sri Lanka-China Social and Cultural Cooperation in China

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Voice of Friendship 2009年第4期 31-33页

作者： tu lili

Invited by the CPAFFC, a delegation of the Association for Sri Lanka-China Social and Cultural Cooperation (ASLCSCC) led by its President Indrananda Abeysekera

关键词： delegation visited exhibition director friendly be

Lin Songtian: China's success is the UN's success

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Voice of Friendship 2022年第3期 1-2页

作者： tu lili 不详

On Aug 1,Lin Songtian,president of the Chinese People’s Association for Friendship with Foreign Countries,met with Siddharth Chatterjee,the UN’s resident coordinator in *** two sides had friendly and indepth exchanges on the United Nations’contribution to China’s development,China’s achievements in development,the country’s policies for cooperation with Africa and their results and other topics of common interest.

关键词： exchanges friendly Africa