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The characterization of transmembrane protein 59-like(TMEM59L)reveals its role in the regulating the level of the GDI protein family

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BIOCELL 2022年第12期46卷 2615-2624页

作者： HAIFENG WANG JUAN GUO tieqiao wen School of Environmental Engineering Central Plains Specialty Food Engineering&Technology Research CenterKaifeng Key Laboratory of Food Composition and Quality AssessmentYellow River Conservancy Technical InstituteKaifeng475004China Laboratory of Molecular Neural Biology School of Life SciencesShanghai UniversityShanghai200444China

The characterization and functions of transmembrane protein 59-like(TMEM59L),a type I transmembrane protein,are not clearly understood until now.Some TMEM59L and fluorescent fusion proteins constructs were transfected in cell lines and liposomes,and their localization was observed.The effects of protein constructs were studied by fluorescence microscopy and western blotting.This study reports a novel function of human TMEM59L(hTMEM59L)related to the expression and location of some proteins.In addition,we report two novel splice variants of human TMEM59L(hTMEM59L).The localization of mutants of this protein,lacking a middle region,and a Cterminal deletion,markedly differed from that of full-length hTMEM59L.Intracellular movement assessment in living cells showed the localization of TMEM59L to vesicular structures in the Golgi bodies,and the cell membrane was observed in living cells.Overexpression of TMEM59L markedly increased the level of amyloid precursor protein because of TMEM59L-mediated inhibition of cell membrane transport but did not affect the expression of betasecretase 2.TMEM59L overexpression also significantly increased the levels of Rab GDP dissociation inhibitor alpha and Rab GDP dissociation inhibitor beta proteins.These results suggest that TMEM59L is involved in the packaging of acidic vesicles and thereby functions in the trafficking and processing of intracellular proteins.

关键词： TMEM59L Localization GDI1 GDI2 Protein processing

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Potential glioblastoma biomarkers identified by mass spectroscopy and iTRAQ labeling

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Genes & Diseases 2023年第5期10卷 1767-1770页

作者： Ruili Feng Mingyu Chen Guanghong Luo Yanlu Chen Jiao Wang Jian Xu Ming Xu Ping Zhong tieqiao wen Laboratory of Molecular Neural Biology School of Life SciencesShanghai UniversityShanghai 200444China Department of Neurosurgery Huashan HospitalShanghai Medical CollegeFudan UniversityShanghai 200040China Neurosurgical Institute of Fudan University Shanghai 200040China Shanghai Clinical Medical Center of Neurosurgery Shanghai 200040China Shanghai Key Laboratory of Brain Function and Restoration and Neural Regeneration Shanghai 200040China

Glioblastoma multiforme(GBM)is the most common primary malignant tumor of the central nervous system.Grade IV GBM is lethal,and has a high rate of relapse despite aggressive therapy.The prognosis is poor because of therapeutic resistance and tumor relapse.1 Very low rates of clinical responses and frequent treatment failures are common.Previous studies have focused on identifying genetic alterations in GBM that have diagnostic and prognostic values and may help to define the subclasses of the GBM patients,2,3 including mutations in isocitrate dehydrogenase 1(IDH1),amplification of epidermal growth factor receptors(EGFR),and mutations and polymorphisms of telomerase reverse transcriptase(TERT)promoter.These markers are useful for identifying the pathogenesis of malignant glioma formation,but they are all associated with tumorigenesis and do not currently influence the treatment of most GBM patients.

关键词： alterations malignant prognosis

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Dendritic Cell Factor 1-Knockout Results in Visual Deficit Through the GABA System in Mouse Primary Visual Cortex

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Neuroscience Bulletin 2018年第3期34卷 465-475页

作者： Jieyun Shi Qian Li tieqiao wen Laboratory of Molecular Neural Biology School of Life Sciences Shanghai University

The visual system plays an important role in our daily life. In this study, we found that loss of dendritic cell factor 1（DCF1） in the primary visual cortex（V1） caused a sight deficit in mice and induced an abnormal increase in glutamic acid decarboxylase 67, an enzyme that catalyzes the decarboxylation of glutamate to gamma aminobutyric acid and CO2, particularly in layer 5. In vivo electrophysiological recordings confirmed a decrease in delta, theta,and beta oscillation power in DCF1-knockout mice. This study presents a previously unknown function of DCF1 in V1, suggests an unknown contact between DCF1 and GABA systems, and provides insight into the mechanism and treatment of visual deficits.

关键词： DCF1 Sight GABA GAD67

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Gene expression profile of Sox1,Sox2,p53,Bax and Nestin in neural stem cells and adult mouse brain tissues

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BIOCELL 2019年第2期43卷 59-64页

作者： HAIFENG WANG KAI WANG JUAN GUO tieqiao wen School of Environmental Engineering Central Plains Specialty Food Engineering&Technology Research CenterKaifeng Key Laboratory of Food Composition and Quality AssessmentYellow River Conservancy Technical InstituteDong Jing AvenueKaifeng475004China Laboratory of Molecular Neural Biology School of Life SciencesShanghai University99 Shang Da RoadShanghai200444China

Histone deacetylation is a key modulator involved in cell proliferation,apoptosis,and mRNA transcription.However,the effects of histone deacetylation on C17.2 neural stem cells(NSCs)remain unclear.Here,the histone deacetylase inhibitors nicotinamide and trichostatin A(TSA)were used to determine the role of histone deacetylation on gene transcription in NSCs.The results showed that the mRNA expression of p53,Sox1,Sox2,and Bax were significantly higher in E14.5 NSCs than in C17.2 NSCs.Nestin,a marker gene of neuronal differentiation,did not differ significantly between E14.5 NSCs and C17.2 NSCs.The transcription levels of p53 and Nestin were significantly higher in C17.2 NSCs than in differentiated brain tissues,and the expression of Bax,Sox1,and Sox2 was higher in the olfactory bulb than in other brain tissues.Nicotinamide and TSA treatment decreased the transcription of Sox2,p53,Nestin,and Bax in C17.2 NSCs,although the difference was statistically significant only for Sox2 and Nestin,Sox1 transcription was not detected.These results demonstrated that mRNA expression profiles differ between C17.2 NSCs,E14.5 NSCs,and adult mouse brain tissues,and HDAC inhibitors regulate gene expression by modulating histone acetylation.

关键词： Histone deacetylase Inhibitors Brain tissues Transcription profile C17.2 neural stem cells

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System approaches reveal the molecular networks involved in neural stem cell differentiation

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Protein & Cell 2012年第3期3卷 213-224页

作者： Kai Wang Haifeng Wang Jiao Wang Yuqiong Xie Jun Chen Huang Yan Zengrong Liu tieqiao wen Laboratory of Molecular Neurobiology School of Life SciencesShanghai UniversityShanghai 200444China School of Communication and Information Engineering Shanghai UniversityShanghai 200444China College of Life Science Wuhan UniversityWuhan 430072China College of Chemical Engineering and Technology Wuhan University of Science and TechnologyWuhan 430081China Institute of Systems Biology Shanghai UniversityShanghai 200444China􀀍

The self-renewal and multipotent potentials in neural stem cells(NSCs)maintain the normal physiological functions of central nervous system(CNS).The ab-normal differentiation of NSCs would lead to CNS disorders.However,the mechanisms of how NSCs differentiate into astrocytes,oligodendrocytes(OLs)and neurons are still unclear,which is mainly due to the complexity of differentiation processes and the limita-tion of the cell separation method.In this study,we modeled the dynamics of neural cell interactions in a systemic approach by mining the high-throughput genomic and proteomic data,and identified 8615 genes that are involved in various biological processes and functions with significant changes during the differentiation processes.A total of 1559 genes are specifically expressed in neural cells,in which 242 genes are NSC specific,215 are astrocyte specific,551 are OL specific,and 563 are neuron specific.In addition,we proposed 57 transcriptional regulators specifically expressed in NSCs may play essential roles in the development courses.These findings provide more comprehensive analysis for better understanding the endogenous mechanisms of NSC fate determination.

关键词： neural stem cell cell differentiation mo-lecular networks

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ATP11B deficiency leads to impairment of hippocampal synaptic plasticity

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Journal of Molecular Cell Biology 2019年第8期11卷 688-702页

作者： Jiao Wang Weihao Li Fangfang Zhou Ruili Feng Fushuai Wang Shibo Zhang Jie Li Qian Li Yajiang Wang Jiang Xie tieqiao wen Laboratory of Molecular Neural Biology School of Life Sciences Shanghai University Shanghai 200444 China School of Computer Engineering and Science Shanghai University Shanghai 200444 China

Synaptic plasticity is known to regulate and support signal transduction between neurons, while synaptic dysfunction contributes to multiple neurological and other brain disorders;however, the specific mechanism underlying this process remains unclear. In the present study, abnormal neural and dendritic morphology was observed in the hippocampus following knockout of Atpllb both in vitro and in vivo. Moreover, ATP11B modified synaptic ultrastructure and promoted spine remodeling via the asymmetrical distribution of phosphatidylserine and enhancement of glutamate release, glutamate receptor expression, and intracellular Ca^2+ concentration. Fuithermoe experimental results also indicate that ATP11B regulated synaptic plasticity in hippocampal neurons through the MAPK14 signaling pathway. In conclusion, our data shed light on the possible mechanisms underlying the regulation of synaptic plasticity and lay the foundation for the exploration of proteins involved in signal transduction during this process.

关键词： ATP11B synaptic plasticity glutamate receptors MAPK14 signaling pathway

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Analysis of Signaling Pathway for the Differentiation of Neural Stem Cells

Analysis of Signaling Pathway for the Differentiation of Neu...

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第一届最优化与系统生物学国际研讨会

作者： Wei Lu Jiao Wang tieqiao wen Institute of Systems Biology Shanghai University School of Life Sciences Shanghai University

G13 signaling pathway is closely related with differentiation of neural stem cells. Rho-GDIγ is a key factor in this pathway. When Rho-GDIγ is silenced by using RNAi, the neural stem cell appears distinguished morphological change. The results indicate that the silenced cells showed the tendency of stimulative differentiation of neural stem cells.

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Rho-GDIγ inhibits the proliferation of human glioma cells

Rho-GDIγ inhibits the proliferation of human glioma cells

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第二届最优化与系统生物学国际研讨会

作者： Jiao Wang Xiao Li Xing-Ming Zhao tieqiao wen Institute of Systems Biology Shanghai University Laboratory of Molecular Neural Biology School of life sciences Shanghai University

Rho-GDIγ, one of GDP-dissociation inhibitors （GDIs） in the GTPases Rho-subfamily, plays a key role in modulating the activation of GTPases, and contributes to reorganization of the actin cytoskeleton, cell aggregation, cell-to-cell adhesion, membrane ruffling, cell motility, and so on. This study primarily shows that Rho-GDIγ inhibits the proliferation of human glioma cells （HGC） U251. The result indicates that overexpression of Rho-GDIγ in U251 significantly inhibits cell proliferation and migration. Bioinformatics analyses indicate that Rho-GDIγ in mouse and human have the similar motifs, domains and structures. The results suggest that Rho-GDIγ in human may be a regulation target in controlling the proliferation of human glioma cells.

关键词： Rho-GDIγ Glioma cells

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Thermodynamic theory of flotation for a complex multiphase solid -liquid system and high-entropy flotation

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International Journal of Minerals,Metallurgy and Materials 2024年第6期31卷 1177-1197页

作者： Shuming wen State Key Laboratory of Complex Nonferrous Metal Resources Clean Utilization Faculty of Land Resource EngineeringKunming University of Science and TechnologyKunming 650093China

The flotation of complex solid–liquid multiphase systems involve interactions among multiple components,the core problem facing flotation theory.Meanwhile,the combined use of multicomponent flotation reagents to improve mineral flotation has become an important issue in studies on the efficient use of refractory mineral resources.However,studying the flotation of complex solid–liquid systems is extremely difficult,and no systematic theory has been developed to date.In addition,the physical mechanism associated with combining reagents to improve the flotation effect has not been unified,which limits the development of flotation theory and the progress of flotation technology.In this study,we applied theoretical thermodynamics to a solid–liquid flotation system and used changes in the entropy and Gibbs free energy of the reagents adsorbed on the mineral surface to establish thermodynamic equilibrium equations that de-scribe interactions among various material components while also introducing adsorption equilibrium constants for the flotation reagents adsorbed on the mineral surface.The homogenization effect on the mineral surface in pulp solution was determined using the chemical potentials of the material components of the various mineral surfaces required to maintain balance.The flotation effect can be improved through synergy among multicomponent flotation reagents;its physical essence is the thermodynamic law that as the number of compon-ents of flotation reagents on the mineral surface increases,the surface adsorption entropy change increases,and the Gibbs free energy change of adsorption decreases.According to the results obtained using flotation thermodynamics theory,we established high-entropy flotation theory and a technical method in which increasing the types of flotation reagents adsorbed on the mineral surface,increasing the adsorption entropy change of the flotation reagents,decreasing the Gibbs free energy change,and improving the adsorption efficiency and stability of the flotation reagents improve

关键词： multicomponent flotation reagent adsorption entropy change equations of thermodynamic equilibrium adsorption equilibri-um constant high-entropy flotation

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Goal-Oriented Control Systems(GOCS):From HOW to WHAT

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IEEE/CAA Journal of Automatica Sinica 2024年第4期11卷 816-819页

作者： wen-Hua Chen the Department of Aeronautical and Automotive Engineering Loughborough UniversityLeicestershireLE113TUU.K.

Brief:New control theory is required to underpin safe design and deployment of future highly automated systems to deal with uncertain environments and complicated tasks,enabled by AI and other advanced technologies.Goal-Oriented Control Systems offer potential to transform the control system design from currently instructing a control system how to perform a task to specifying what is to be achieved.

关键词： automated instru system

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