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Is the kafirin profile capable of modulating the ileal digestibility of amino acids in a soybean meal-sorghum diet fed to pigs?

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Animal Nutrition 2019年第2期5卷 124-129页

作者： Víctor A.Balderrama-Pérez JoséG.Gómez-Soto tércia c.reis de souza Ericka R.Rodríguez Gerardo Mariscal-Landín Facultad de Estudios Superiores cuatitlán Universidad National Autónoma de MéxicoCuautitlan54714Mexico Facultad de cientias Naturales Universidad Autónoma de QuerétaroQuerétaro76230Mexico centro Nacional de Investigación Disciplinaria en Fisiología y Mejoramiento Animal Instituto Nacional de Investigaciones Forestales Agrícolas y PecuariasAjuchitilan76380Mexico

the effects of kafirins on protein and amino acid ileal digestibility have not been evaluated in vivo in *** aim of this study was to determine the effects of protein profile on apparent ileal digestibility(AID)of amino acids. We used a sorghum hybrid with low tannin content(the same hybrid was harvested from 2 different plots with different kafirin profile. Sorghum with greater content of total kafirins had less content of y-and al-kafirins and higher content of β-and a2-kafirins than that with lower content of total kafirins. two sorghum-soybean meal(SBM) diets were formulated: 1) low kafirin(LK) content(32.2 g/kg) and 2) high kafirin(HK) content(48.1 g/kg). A control diet(maize-SBM) and a reference SBM-diet were also prepared. the reference diet was fed to all pigs following the experimental period and was used to estimate the AID of cereals by the difference method. 't' cannulas were fixed in the distal ileum of 18 barrows(6 by treatment), divided into 2 groups of 9 pigs. the pigs were fed 2.5 times their maintenance requirement of digestible energy(110 kcal/kg BW00.75). the AID of dry matter,protein, amino acids, and energy of the experimental diets was measured;the AID of cereals(maize, LK sorghum and HK sorghum) was estimated by the difference method. the maize-SBM diet was more digestible than the sorghum-SBM diets, only with respect to valine(P the AID of valine in the maize-SBM diet was higher than that in sorghum-SBM diets. the changes in kafirin profile between the diets only affected the AID of threonine(P ch decreased by 9.5 percentage units in LK diet compared with HK diet. Regarding the AID of cereals, maize exhibited greater AID than sorghum, with respect to valine(P comparison of sorghum with LK and HK content showed that the AID of threonine and serine increased by 50.5(P centage units(P the latter, respec:tively. the higher content of γ-kafirins in L

关键词： Amino acids Ileal digestibility Kafirin Pig Sorghum

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What is the effect of tribulus terrestris on testicular morphology and sperm production?An experimental study in the rat model

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Asian Journal of Andrology 2024年第1期26卷 67-70页

作者： Marcello Henrique Araujo Da Silva Gabriela Faria Buys-Goncalves Joao Henrique Duque reis Estrada Francisco Jose Barcellos Sampaio Diogo Benchimol de souza Urogenital Research Unit Rio de Janeiro State UniversityRio de Janeiro 20551-030RJBrazil

the objective of this study was to investigate whether treatment with tribulus terrestris(tt)has any impact on the testicular morphology and function in a rodent *** male rats were divided into a control group and a group receiving 100 mg kg^(-1) body weight of tt *** 4o days of experiment,the animals were submitted to euthanasia;epididymal tail spermatozoa were collected;and spermatozoa concentration,motility,and viability were *** addition,testicles were collected and processed for histomorphometrical *** were compared using the Student's t-test and considered significant when Pconcentration,motility,and viability showed no difference between the ***,testicular weight and volume,seminiferous tubule diameter,tunica propria surface density,seminiferous epithelium surface density,and intertubular compartment surface density were statistically similar between the ***,seminiferous epithelium height and tubular lumen surface density were augmented in animals treated with *** with tt does not cause a major impact on testicular morphology,promoting only subtle *** difference on spermatozoa parameters was observed.

关键词： morphometry testicle tribulus terrestris

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Effect of resistance training volume on body adiposity,metabolic risk,and inflammation in postmenopausal and older females:Systematic review and meta-analysis of randomized controlled trials

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Journal of Sport and Health Science 2024年第2期13卷 145-159页

作者： Paulo Ricardo Prado Nunes Pamela castro-e-souza Anselmo Alves de Oliveira Bruno de Freitas camilo Gislaine cristina-souza Lucio Marques Vieira-souza Marcelo Augusto da Silva carneiro department of Body and Human Movement Minas Gerais State University(UEMG)37902-092 PassosMGBrazil University center of Planalto de Araxa(UNIARAXA) 38180-129 AraxaMGBrazil Physical Activity Health and Ageing Research Group(GPASE)Minas Gerais State University(UEMG)37902-092 PassosMGBrazil Exercise and Nutrition Research Group(GPEN) Minas Gerais State University(UEMG)37902-092 PassosMGBrazil Sport Sciences and Physical Exercise Study and Research Group(GEPcEEX) Minas Gerais State University(UEMG)37902-092 PassosMGBrazil Metabolism Nutrition and Exercise LaboratoryPhysical Education and Sport CenterLondrina State University(UEL)86050-070 LondrinaPRBrazil Postgraduate Program in Physical Education Federal University of Sergipe(UFS)49107-230 Sao CristovaoSEBrazil

Purpose:this meta-analytical study aimed to explore the effects of resistance training(Rt) volume on body adiposity,metabolic risk,and inflammation in postmenopausal and older ***:A systematic search was performed for randomized controlled trials in PubMed,Scopus,Web of Science,and *** controlled trials with postmenopausal and older females that compared Rt effects on body adiposity,metabolic risk,and inflammation with a control group(cG) were *** reviewers selected the studies,extracted the data,and performed the risk of bias and certainty of the evidence(Grading of Recommendations,Assessment,development,and Evaluation(GRAde)) *** body and abdominal adiposity,blood lipids,glucose,and c-reactive protein were included for meta-analysis.A random-effects model,standardized mean difference(Hedges’ g),and 95% confidence interval(95%cI) were used for ***:twenty randomized controlled trials(overall risk of bias:some concerns;GRAde:low to very low) with overweight/obese postmenopausal and older females were *** groups were divided into low-volume Rt(LVRt,~44 sets/week) and high-volume Rt(HVRt,~77 sets/week).Both Rt groups presented improved body adiposity,metabolic risk,and inflammation when compared to ***,HVRt demonstrated higher effect sizes than LVRt for glucose(HVRt=-1.19;95%cI:-1.63 to-0.74;LVRt=-0.78;95%cI:-1.15 to-0.41) and c-reactive protein(HVRt=-1.00;95%cI:-1.32 to-0.67;LVRt=-0.34;95%cI,-0.63 to-0.04)) when compared to ***:compared to cG,HVRt protocols elicit greater improvements in metabolic risk and inflammation outcomes than LVRt in overweight/obese postmenopausal and older females.

关键词： c-reactive protein Fat mass Lipid profile Menopause Strength training

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the effect of abiotic stresses on plant c:N:P homeostasis and their mitigation by silicon

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the crop Journal 2024年第2期12卷 340-353页

作者： Milton Garcia costa Renato de Mello Prado Luiz Fabiano Palaretti Jonas Pereira de souza Júnior department of Agricultural Production Science School of Agricultural and Veterinarian SciencesSão Paulo State UniversityJaboticabalBrazil

In crop plants, various environmental stresses affect the balance of carbon, nitrogen, and phosphorus(c:N:P), leading to biochemical and physiological alterations and reductions in yield. Silicon(Si) is a beneficial element that alleviates plant stress. Most studies involving silicon have focused on physiological responses, such as improvements in photosynthetic processes, water use efficiency, and antioxidant defense systems. But recent research suggests that stressed plants facing either limited or excessive resources(water, light, nutrients, and toxic elements), strategically employ Si to maintain c:N:P homeostasis, thereby minimizing biomass losses. Understanding the role of Si in mitigating the impact of abiotic stresses on plants by regulating c:N:P homeostasis holds great potential for advancing sustainable agricultural practices in crop production. this review presents recent advances in characterizing the influence of environmental stresses on c:N:P homeostasis, as well as the role of Si in preserving c:N:P equilibrium and attenuating biological damage associated with abiotic stress. It underscores the beneficial effects of Si in sustaining c:N:P homeostasis and increasing yield via improved nutritional efficiency and stress mitigation.

关键词： Nutritional stoichiometry Elemental stoichiometry Beneficial element Environmental stresses Nutritional efficiency carbon use efficiency Agricultural sustainability

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Urea breath test for Helicobacter pylori infection in adult dyspeptic patients: A meta-analysis of diagnostic test accuracy

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World Journal of Gastroenterology 2024年第6期30卷 579-598页

作者： Fabian Fellipe Bueno Lemos caroline tianeze de castro Marcel Silva Luz Gabriel reis Rocha Gabriel Lima correa Santos Luís Guilherme de Oliveira Silva Mariana Santos calmon cláudio Lima souza Ana carla Zarpelon-Schutz Kádima Nayara teixeira Dulciene Maria de Magalhães Queiroz Fabrício Freire de Melo Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil Instituto de Saúde coletiva Universidade Federal da BahiaSalvador 40110040BahiaBrazil campus toledo Universidade Federal do ParanáToledo 85919-899ParanáBrazil Laboratory of Research in Bacteriology UFMGBelo Horizonte 30130-100Minas GeraisBrazil

BAcKGROUND Helicobacter pylori(***)infection has been well-established as a significant risk factor for several gastrointestinal *** urea breath test(UBt)has emerged as a leading non-invasive method for detecting *** numerous studies confirming its substantial accuracy,the reliability of UBt results is often compromised by inherent *** findings underscore the need for a rigorous statistical synthesis to clarify and reconcile the diagnostic accuracy of the UBt for the diagnosis of *** *** to determine and compare the diagnostic accuracy of 13c-UBt and 14c-UBt for *** infection in adult patients with *** We conducted an independent search of the PubMed/MEDLINE,EMBASE,and cochrane central databases until April *** search included diagnostic accuracy studies that evaluated at least one of the index tests(^(13)c-UBt or ^(14)c-UBt)against a reference *** used the QUADAS-2 tool to assess the methodo-logical quality of the *** utilized the bivariate random-effects model to calculate sensitivity,specificity,positive and negative test likelihood ratios(LR+and LR-),as well as the diagnostic odds ratio(DOR),and their 95%confidence *** conducted subgroup analyses based on urea dosing,time after urea administration,and assessment *** investigate a possible threshold effect,we conducted Spearman correlation analysis,and we generated summary receiver operating characteristic(SROc)curves to assess ***,we visually inspected a funnel plot and used Egger’s test to evaluate publication *** both as reliable diagnostic tools in clinical *** In summary,our study has demonstrated that ^(13)c-UBt has been found to outperform the ^(14)c-UBt,making it the preferred diagnostic ***,our results emphasize the significance of carefully considering urea dosage,assessment timing,and measurement techniques for both tests to enh

关键词： Helicobacter pylori Urea breath test Diagnosis Diagnostic test accuracy Meta-analysis

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Adsorption of yttrium(Y^(3+)) and concentration of rare earth elements from phosphogypsum using chitin and chitin aerogel

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Journal of Rare Earths 2024年第4期42卷 775-782页

作者： Glaydson S.dos reis Diana Pinto Sabrina F.Lütke Eder c.Lima Luis F.O.Silva Irineu A.S.de Brum Guilherme L.Dotto department of Forest Biomaterials and technology Biomass technology centre Swedish University of Agricultural SciencesUmeaE13Swedn Universidad de La costa Calle 58#55-66080002BarranquillaAtlanticoColombia Research Group on Adsorptive and catalytic Process Engineering(ENGEPAc) Federal University of Santa MariaAv.Roraima100079705900Santa MariaRSBrazil Institute of chemistry Federal University of Rio Grande do Sul90040060Porto AlegreBrazil

chitin powder and chitin-aerogel were prepared from shrimp wastes and used to uptake Y^(3+)from aqueous solutions and concentrate this rare earth element from phosphogypsum(PG).chitin aerogel displays a specific surface area of 945 m^(2)/g,while chitin powder is 3.6 m^(2)/g,which largely influences its adsorption *** the adsorption in synthetic solutions,the effect of pH on Y^(3+)removal is strong for chitin powder *** contrast,no big pH influence was detected for chitin *** interactions and chelation can highlight the proposed mechanism of Y^(3+)on chitin adsorbents for the powder and ***,in addition to these interactions,pore filling/pore diffusion is the main mechanism of Y^(3+)removal in the chitin *** aerogel is efficient in concentrating 8 times the Y^(3+)from PG,a very complex *** complex chitin aerogel-Y^(3+)can be a secondary source of rare earth elements for other applications.

关键词： Rare earths Sustainable material chitin powder chitin porous aerogel Yttrium adsorption and recovery

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Rifaximin on epigenetics and autophagy in animal model of hepatocellular carcinoma secondary to metabolic-dysfunction associated steatotic liver disease

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World Journal of Hepatology 2024年第1期16卷 75-90页

作者： Matheus truccolo Michalczuk Larisse Longo Melina Belén Keingeski Bruno de souza Basso Gabriel tayguara Silveira Guerreiro Jessica t Ferrari JoséEduardo Vargas cláudia P Oliveira carolina Uribe-cruz carlos thadeu Schmidt cerski Eduardo Filippi-chiela Mário reisÁlvares-da-Silva Division of Gastroenterology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-903Rio Grande do SulBrazil Graduate Program in Gastroenterology and Hepatology Universidade Federal do Rio Grande do SulPorto Alegre 90035-003Rio Grande do SulBrazil Experimental Laboratory of Hepatology and Gastroenterology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-903Rio Grande do SulBrazil Laboratory of Inflammatory and Neoplastic cells Universidade Federal do ParanáParaná81530900Brazil department of Gastroenterology(LIM07) Faculdade de Medicina da Universidade de São PauloSão Paulo 01246903Brazil Facultad de ciencias de la Salud Universidad Católica de las MisionesPosadasMisiones 3300Argentina Unit of Surgical Pathology Hospital de Clinicas de Porto AlegrePorto Alegre 90035-903Rio Grande do SulBrazil center of Biotechnology Universidade Federal do Rio Grande do SulPorto Alegre 90035-003Rio Grande do SulBrazil department of Morphological Sciences Universidade Federal do Rio Grande do SulPorto Alegre 90.050-170Rio Grande do SulBrazil conselho Nacional de desenvolvimento científico e tecnológico(cNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq)ResearcherBrasília 71.605-001Brazil

BAcKGROUND Prevalence of hepatocellular carcinoma(Hcc)is increasing,especially in patients with metabolic dysfunctionassociated steatotic liver disease(MASLD).AIM to investigate rifaximin(RIF)effects on epigenetic/autophagy markers in *** Adult Sprague-Dawley rats were randomly assigned(n=8,each)and treated from 5-16 wk:control[standard diet,water plus gavage with vehicle(Veh)],Hcc[high-fat choline deficient diet(HFcD),diethylnitrosamine(deN)in drinking water and Veh gavage],and RIF[HFcD,deN and RIF(50 mg/kg/d)gavage].Gene expression of epigenetic/autophagy markers and circulating miRNAs were *** All Hcc and RIF animals developed metabolic-dysfunction associated steatohepatitis fibrosis,and cirrhosis,but three RIF-group did not develop *** animals who developed Hcc with those who did not,miR-122,miR-34a,tubulin alpha-1c(tuba-1c),metalloproteinases-2(Mmp2),and metalloproteinases-9(Mmp9)were significantly higher in the *** opposite occurred with Becn1,coactivator associated arginine methyltransferase-1(carm1),enhancer of zeste homolog-2(Ezh2),autophagy-related factor Lc3A/B(Map1 Lc3b),and p62/sequestosome-1(p62/SQStM1)-*** with controls,Map1 Lc3b,Becn1 and Ezh2 were lower in Hcc and RIF-groups(Pcarm1 was lower in Hcc compared to RIF(Ptic expression of Mmp9 was higher in Hcc in relation to the control;the opposite was observed for p62/Sqstm1(PtM1 protein was lower in the RIF-group compared to the control(P=0.024).there was no difference among groups for tuba-1c,Aldolase-B,alpha-fetoprotein,and Mmp2(P>0.05).miR-122 was higher in Hcc,and miR-34a in RIF compared to controls(Pcc compared to RIF,and the inverse was observed for miR-224(Pthere was no difference among groups regarding miR-33a,miR-143,miR-155,miR-375 and miR-21(P>0.05).cONcLUSION RIF might have a possible beneficial effect on preventing/delaying liver carcinogenesis throu

关键词： Animal model Autophagy Epigenetic Hepatocellular carcinoma Metabolic dysfunction-associated steatotic liver disease Rifaximin

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concerns about the application of resistance exercise with blood-flow restriction and thrombosis risk in hemodialysis patients

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Journal of Sport and Health Science 2024年第4期13卷 548-558,F0003页

作者： Hugo de Luca correa Lysleine Alves deus Dahan da cunha Nascimento Nicholas Rolnick Rodrigo Vanerson Passos Neves Andrea Lucena reis thais Branquinho de Araujo carmen tzanno-Martins Fernanda Silveira tavares Luiz Sinesio Silva Neto claudio Avelino Rodrigues Santos Paolo Lucas Rodrigues-Silva Fernando Honorato souza Vitoria Marra da Motta Vilalva Mestrinho Rafael Lavarini dos Santos Rosangela Vieira Andrade Jonato Prestes thiago dos Santos Rosa Post-graduate Program of Physical Education Catholic University of BrasıliaBrasılia 71966-700Brazil the Human Performance Mechanic Lehman CollegeNew YorkNY 10011USA clinical Group Home Dialysis center and Renalclass Sao Paulo 01239-040Brazil department of Medicine Catholic University of BrasıliaBrasılia 71966-700Brazil Faculty of Medicine Federal University of TocantinsTocantins 77001-090Brazil Embrapa Genetic Resources and Biotechnology Brasılia 70770-917Brazil Graduate Program of Genomic Sciences and Biotechnology Brasılia 71966-700Brazil

Background:Hemodialysis(HD) per se is a risk factor for *** the growing body of evidence on blood-flow restriction(BFR) exercise in HD patients,identification of possible risk factors related to the prothrombotic agent D-dimer is required for the safety and feasibility of this training *** aim of the present study was to identify risk factors associated with higher D-dimer levels and to determine the acute effect of resistance exercise(RE) with BFR on this ***:two hundred and six HD patients volunteered for this study(all with a glomerular filtration rate of the RE+BFR session consisted of 50% arterial occlusion pressure during 50 min sessions of HD(intradialytic exercise).RE repetitions included concentric and eccentric lifting phases(each lasting 2 s) and were supervised by a strength and conditioning ***:Several variables were associated with elevated levels of D-dimer,including higher blood glucose,citrate use,recent cardiovascular events,recent intercurrents,higher inflammatory status,catheter as vascular access,older patients(>70 years old),and HD ***,RE+BFR significantly increases D-dimer after 4 *** with borderline baseline D-dimer levels(400-490 ng/mL) displayed increased risk of elevating D-dimer over the normal range(≥500 ng/mL).conclusion:these results identified factors associated with a heightened prothrombotic state and may assist in the screening process for HD patients who wish to undergo RE+BFR.D-dimer and/or other fibrinolysis factors should be assessed at baseline and throughout the protocol as a precautionary measure to maximize safety during RE+BFR.

关键词： Blood-flow restriction chronic kidney disease coagulation Hemodialysis Vascular occlusion exercises

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Liver transplantation as an alternative for the treatment of perihilar cholangiocarcinoma: A critical review

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Hepatobiliary & Pancreatic Diseases International 2024年第2期23卷 139-145页

作者： Wellington Andraus Francisco tustumi Alexandre chagas Santana Rafael Soares Nunes Pinheiro Daniel reis Waisberg Liliana Ducatti Lopes Rubens Macedo Arantes Vinicius Rocha Santos Rodrigo Bronze de Martino Luiz Augusto carneiro D’Albuquerque department of Gastroenterology Transplantation UnitUniversidade de São PauloSão PauloBrazil

Background:Perihilar cholangiocarcinoma(phccc)is a dismal *** is no consensus regard-ing the best treatment for patients with unresectable *** present review aimed to gather the current pieces of evidence for liver transplantation and liver resection as a treatment for phccc and to build better guidance for clinical *** sources:the search was conducted in PubMed,Embase,cochrane,and *** related references were searched *** criteria were:reports in English or Portuguese literature that a)patients with confirmed diagnosis of phccc;b)patients treated with a curative intent;c)patients with the outcomes of liver resection and liver *** reports,reviews,letters,editorials,conference abstracts and papers with full-text unavailability were excluded from the ***:Most of the current literature is based on observational retrospective studies with low grades of *** resection has better long-term outcomes than systemic chemotherapy or palliation ther-apy and liver transplantation is a good alternative for selected patients with unresectable *** candidates for resection or transplantation should be medically fit and free of intrahepatic or extrahep-atic *** a general rule,patients presenting with a tumor having a longitudinal size>3 cm or extending below the cystic duct,lymph node disease,confirmed extrahepatic dissemination;intraoper-atively diagnosed metastatic disease;a history of other malignancies within the last five years,and did not complete chemoradiation regimen and were medically unfit should not be considered for *** of these criteria should be individually *** transplantation or resection should only be considered in highly experienced hepatobiliary centers,and any decision-making must be based on a multidisciplinary ***:phccc is a complex condition with high *** therapies,including hepatec-tomy and liv

关键词： Liver transplantation cholangiocarcinoma Liver neoplasms

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Estimation of endogenous protein and amino acid ileal losses in weaned piglets by regression analysis using diets with graded levels of casein

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Journal of Animal Science and Biotechnology 2014年第1期5卷 74-79页

作者： tércia cesária reis de souza Araceli Aguilera Barreyro Gerardo Mariscal-Landín Facultad de ciencias Naturales Universidad Autónoma de QuerétaroSantiago de Querétaro Av. De las Ciencias s/n Juriquilla 76000 QuerétaroQuerétaro México centro Nacional de Investigación en Fisiología Animal Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias Km 1Carretera a Colón Ajuchitlán Colón Querétaro 76280 México

Background： Many studies have investigated endogenous loss of proteins and amino acids （AAs） at the ileal level in growing pigs. However, only a few studies have researched this subject in piglets. Knowledge regarding AA ileal digestibility in piglets would be helpful during the formulation of diets for weaning piglets, rather than just using coefficients obtained in growing pigs. therefore, in this study, we sought to estimate endogenous protein and AA ileal losses in piglets. Furthermore, apparent and true ileal digestibility （AID and tID） of protein and AAs from casein were measured. Results： the average flow of protein was 20.8 g/kg of dry matter intake （DMI）. Basal protein loss, as estimated by regression, was 16.9 g/kg DMI. Glutamic acid, arginine, and aspartic acid （2.2, 1.4, and 1.2 g/kg DMI, respectively） were the AAs for which greater losses were seen. the AID of protein and AAs increased as the protein level in the diet increased. A higher increment in AID was observed between diets with 80 and160 g cP/kg of feed; this finding was mainly attributable to increases in glycine and arginine （46.1% and 18%, respectively）. the tID of protein was 97.8, and the tID of AAs varied from 93.9 for histidine to 100.2 for phenylalanine. conclusions： the basal endogenous protein loss in piglets was 16.9 g/kg DMI. Endogenous protein was rich in glutamic acid, aspartic acid, and arginine, which represented 32.7% of endogenous protein loss in weaning piglets. the tID of casein was high and varied from 93.0 for histidine to 100.2 for phenylalanine.

关键词： Amino acid loss Ileal digestibility Protein loss Weaned piglets

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