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Induction of CXC chemokines in human mesenchymal stem cells by stimulation with secreted frizzled-related proteins through non-canonical Wnt signaling

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World Journal of Stem Cells 2015年第11期7卷 1262-1273页

作者： David S Bischoff Jian-Hua Zhu Nalini S Makhijani Dean t yamaguchi Research Service VA Greater Los Angeles Healthcare System Department of Medicine David Geffen School of Medicine at UCLA

AIM: to investigate the effect of secreted frizzledrelated proteins(s FRPs) on CXC chemokine expression in human mesenchymal stem cells(h MSCs).MEtHODS: CXC chemokines such as CXCL5 and CXCL8 are induced in h MSCs during differentiation with osteogenic differentiation medium(OGM) and may be involved in angiogenic stimulation during bone repair. h MSCs were treated with conditioned medium(CM) from L-cells expressing non-canonical Wnt5 a protein, or with control CM from wild type L-cells, or directly with s FRPs for up to 10 d in culture. m RNA expression levels of both CXCL5 and CXCL8 were quantitated by real-time reverse transcriptase-polymerase chain reaction and secreted protein levels of these proteins determined by ELISA. Dose-(0-500 ng/m L) and time-response curves were generated for treatment with s FRP1. Signal transduction pathways were explored by western blot analysis with pan- or phosphorylation-specific antibodies, through use of specific pathway inhibitors, and through use of si RNAs targeting specific frizzled receptors(Fzd)-2 and 5 or thereceptor tyrosine kinase-like orphan receptor-2(Ro R2) prior to treatment with s FRPs. RESULtS: CM from L-cells expressing Wnt5 a, a noncanonical Wnt, stimulated an increase in CXCL5 m RNA expression and protein secretion in comparison to control L-cell CM. s FRP1, which should inhibit both canonical and non-canonical Wnt signaling, surprisingly enhanced the expression of CXCL5 at 7 and 10 d. Dickkopf1, an inhibitor of canonical Wnt signaling prevented the s FRPstimulated induction of CXCL5 and actually inhibited basal levels of CXCL5 expression at 7 but not at 10 d post treatment. In addition, all four s FRPs isoforms induced CXCL8 expression in a dose- and time-dependent manner with maximum expression at 7 d with treatment at 150 ng/m L. the largest increases in CXCL5 expression were seen from stimulation with s FRP1 or s FRP2. Analysis of mitogen-activated protein kinase signaling pathways in the presence of OGM sh

关键词： CXC chemokines Mesenchymal stem cell Osteogenesis Differentiation Wnt signaling pathway Frizzled-related protein Frizzled receptors

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WJSC 6^(th) Anniversary Special Issues(2):Mesenchymal stem cells “Ins” and “Outs” of mesenchymal stem cell osteogenesis in regenerative medicine

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World Journal of Stem Cells 2014年第2期6卷 94-110页

作者： Dean t yamaguchi Research Service Veteran Administration Greater Los Angeles Healthcare System and David Geffen School of Medicine at University of California at Los Angeles

Repair and regeneration of bone requires mesenchymal stem cells that by self-renewal,are able to generate a critical mass of cells with the ability to differentiate into osteoblasts that can produce bone protein matrix(osteoid)and enable its *** number of human mesenchymal stem cells(hMSCs)diminishes with age and ex vivo replication of hMSCs has limited *** propagating hMSCs under hypoxic conditions may maintain their ability to self-renew,the strategy of using human telomerase reverse transcriptase(htERt)to allow for hMSCs to prolong their replicative lifespan is an attractive means of ensuring a critical mass of cells with the potential to differentiate into various mesodermal structural tissues including ***,this strategy must be tempered by the oncogenic potential of tERt-transformed cells,or their ability to enhance already established cancers,the unknown differentiating potential of high population doubling hMSCs and the source of hMSCs(e.g.,bone marrow,adipose-derived,muscle-derived,umbilical cord blood,etc.)that may provide peculiarities to self-renewal,differentiation,and physiologic function that may differ from non-transformed native *** engineering approaches to use hMSCs to repair bone defects utilize the growth of hMSCs on three-dimensional scaffolds that can either be a base on which hMSCs can attach and grow or as a means of sequestering growth factors to assist in the chemoattraction and differentiation of native *** use of whole native extracellular matrix(ECM)produced by hMSCs,rather than individual ECM components,appear to be advantageous in not only being utilized as a three-dimensional attachment base but also in appropriate orientation of cells and their differentiation through the growth factors that native ECM harbor or in simulating growth factor *** origin of native ECM,whether from hMSCs from young or old individuals is a critical factor in"rejuvenating"hMSCs from older individuals

关键词： Mesenchymal stem cell telomerase reverse transcriptase Extracellular matrix Osteogenesis Regenerative medicine tissue engineering Proliferation Differentiation

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用液相外延法生长3～7μm波段InAs/InAs_(1-y)Sb_y及其光学和电学性质

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光电子．激光 2012年第2期23卷 286-290页

作者：高玉竹龚秀英 Makino t yamaguchi t Rowell N L 同济大学电子与信息工程学院上海201804 Hamamatsu Photonics K.K. 5000 HirakuchiHamakita 434-8601Japan Research Institute of Electronics Shizuoka University3-5-1 JohokuHamamatsu 432-8011Japan

用液相外延(LPE)法在InAs衬底上生长了3～7μm波段的InAs1-ySby外延层,研究了外延多层的组份与禁带宽度和晶格常数的关系。用光学显微镜、傅立叶变换红外(FtIR)透射、光荧光(PL)谱测试以及偏振光椭圆仪研究了外延材料的光学特性。电学... 详细信息

用液相外延(LPE)法在InAs衬底上生长了3～7μm波段的InAs1-ySby外延层,研究了外延多层的组份与禁带宽度和晶格常数的关系。用光学显微镜、傅立叶变换红外(FtIR)透射、光荧光(PL)谱测试以及偏振光椭圆仪研究了外延材料的光学特性。电学性质是将计算值与实测有效霍尔(Hall)参数的厚度关系拟合得到的。结果表明,本文生长的材料在中红外光伏型探测器上具有良好的应用前景。

关键词： InAs/InAsSb 液相外延(LPE) 光学性质电学性质

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多功能人工心脏控制系统的研制

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国际生物医学工程杂志 1989年第4期 250-251页

作者： yamaguchi t 陈立新

我们已经研制了一种可携带式气动人工心脏控制系统,此系统能自动进行血压控制,虽然是常规泵动方法,然而是非侵入式的。控制系统中的血压是间接测量的,驱动压的逐渐变化其波形图与动脉和静脉压力吻合。驱动压波形是通过电子气压调节器控... 详细信息

我们已经研制了一种可携带式气动人工心脏控制系统,此系统能自动进行血压控制,虽然是常规泵动方法,然而是非侵入式的。控制系统中的血压是间接测量的,驱动压的逐渐变化其波形图与动脉和静脉压力吻合。驱动压波形是通过电子气压调节器控制,而波形图变化是通过光传感器测量。由问

关键词：人工心脏人工器官血压

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Direct mass measurements of neutron-rich ^(86)Kr projectile fragments and the persistence of neutron magic number N=32 in Sc isotopes

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Chinese Physics C 2015年第10期39卷 24-28页

作者：徐星王猛张玉虎徐瑚珊帅鹏涂小林 Yuri A.Litvinov 周小红孙保华原有进夏佳文杨建成 Klaus Blaum 陈瑞九陈相成付超义葛壮胡正国黄文嘉刘大委蓝乙华马新文毛瑞士 t.Uesaka 肖国青邢元明 t.yamaguchi Y.yamaguchi 曾奇颜鑫亮赵红卫赵铁成章卫詹文龙 Key Laboratory of High Precision Nuclear Spectroscopy and Center for Nuclear Matter Science Institute of Modern Physics Chinese Academy of Sciences University of Chinese Academy of Sciences CSNSM-IN2P3-CNRS Université de Paris Sud Research Center for Hadron Physics National Laboratory of Heavy Ion Accelerator Facility in Lanzhou and University of Science and Technology of China GSI Helmholtzzentrum für Schwerionenforschung School of Physics and Nuclear Energy Engineering Beihang University Max-Planck-Institut fr Kernphysik RIKEN Nishina Center Department of Physics Saitama University

In this paper, we present direct mass measurements of neutron-rich S6Kr projectile fragments conducted at the HIRFL-CSR facility in Lanzhou by employing the Isochronous Mass Spectrometry （IMS） method. the new mass excesses of ^52-54Sc nuclides are determined to be -40492（82）, -38928（114）, -34654（540） keV, which show a significant increase of binding energy compared to the reported ones in the Atomic Mass Evaluation 2012 （AME12）. In particular, ^53Sc and ^54sc are more bound by 0.8 MeV and 1.0 MeV, respectively. the behavior of the two neutron separation energy with neutron numbers indicates a strong sub-shell closure at neutron number N=32 in Sc isotopes.

关键词： isochronous mass spectrometry exotic nuclei mass excess new magic number

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免疫或人工感染鸡副嗜血杆菌发生的血清学应答

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国外兽医学（畜禽疾病） 1989年第3期10卷 34-36页

作者： t.yamaguchi 张则斌

免疫或人工感染鸡副嗜血杆菌(Hpg)血清变异型A或C的鸡发生的血清学应答可用特异性的血球凝集(HA)抗原和普通的HA抗原进行检查。免疫或人工感染Hpg血清变异型A的鸡能产生针对Hpg血清变异型的特异性的HA抗原和Hpg普通的HA抗原的血凝抑制(... 详细信息

免疫或人工感染鸡副嗜血杆菌(Hpg)血清变异型A或C的鸡发生的血清学应答可用特异性的血球凝集(HA)抗原和普通的HA抗原进行检查。免疫或人工感染Hpg血清变异型A的鸡能产生针对Hpg血清变异型的特异性的HA抗原和Hpg普通的HA抗原的血凝抑制(HI)抗体;大部分鸡用血清变异型C免疫后第3周能检出两种HA抗原型的HI抗体,以后滴度逐渐下降;大多数鸡人工感染血清变异型C只产生对普通HA抗原的HI抗体,很少的鸡产生对血清变异型的特异性的HA抗原的HI抗体。

关键词：免疫人工感染副嗜血杆菌鸡血清

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新型炭黑/橡胶偶合剂

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橡胶参考资料 1989年第11期19卷 9-18页

作者： t.yamaguchi 鲍青文

1.前言近来,在汽车领域内对橡胶制品有一些迫切要求,例如节省燃油的客车、延长大规格轮胎的运转寿命和降低车内的振动和噪声,这些要求与硫化橡胶中的能量损耗有密切关系。众所周知,在橡胶性能中,弹性、生热性和 tanδ等动态性能都是能... 详细信息

1.前言近来,在汽车领域内对橡胶制品有一些迫切要求,例如节省燃油的客车、延长大规格轮胎的运转寿命和降低车内的振动和噪声,这些要求与硫化橡胶中的能量损耗有密切关系。众所周知,在橡胶性能中,弹性、生热性和 tanδ等动态性能都是能量损耗指数,因此,为了满足这些要求,改进动态性能已变得十分重要。

关键词：炭黑橡胶偶合剂

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偶联剂改善胶料性能

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橡胶参考资料 1991年第9期21卷 26-31页

作者： t.yamaguchi 王瑞芝

前言一种新的非亚硝基的炭黑/橡胶偶联剂—N,N′-双(2-甲基,1-2-硝基丙基)-1,6-二氦基己烷已被研究出来,简称BNAH,商品名称Sumifine 1162。加入这种偶联剂后,诸如回弹性、生热性以及tan δ等动态性能均得到改善。直到现在,亚硝基化合物... 详细信息

前言一种新的非亚硝基的炭黑/橡胶偶联剂—N,N′-双(2-甲基,1-2-硝基丙基)-1,6-二氦基己烷已被研究出来,简称BNAH,商品名称Sumifine 1162。加入这种偶联剂后,诸如回弹性、生热性以及tan δ等动态性能均得到改善。直到现在,亚硝基化合物例如N-(2-硝基丙基)-4-亚硝基苯胺、N-甲基-4,N—二亚硝基苯胺、8—羟基-5-亚硝基喹啉和对—亚硝基二苯胺仍是炭黑/橡胶的偶联剂。由于一些问题,特别是存在亚硝基的问题。

关键词：偶联剂胶料性能硝基苯胺二亚硝基苯亚硝基回弹性拉伸强度热性动态性能硫化胶

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Studies on the exotic structure of 23AI

Studies on the exotic structure of 23AI

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第十三届全国核物理大会暨第八届会员代表大会

作者： M.Hosoi t.Izumikawa R.Kanungo S.Nakajima t.Ohnishi t.Ohtsubo t.Suda K.Sugawara t.Suzuki A.Ozawa A.takisawa K.tanaka t.yamaguchi I.tanihata Department of Physics Saitama UniversitySaitama 338-8570Japan Department of Physics Niigata UniversityNiigata 950-2181Japan tRIUMF 4004 Wesbrook MalVancouverBritish Columbia V6T 2A3Canada Institute of physical and Chemical Research(RIKEN) Wako-shiSaitama 351-8571Japan Department of Physics Tsukuba UniversityIbaraki 305-8571Japan

Since the pioneering measurements of the interaction cross section (σ) and observation of an remarkable large σ for Li,studies on the structure of nuclei far from the β-stability line have been one of the frontiers in nuclear physics for more than two *** has been discovered that there are exotic structure like neutron halo or skin in light neutron-rich nuclei. Researches on the proton-rich nuclei have suggested that B,Ne,Al,p,S may be

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超临界蜂胶提取物的美白效果(英文)

超临界蜂胶提取物的美白效果(英文)

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第八届国际蜂疗大会暨蜂产品保健博览会

作者： R-E Sakai Y-Y takiguchi t-A yamaguchi Department of Life and Environmental Sciences Faculty of Engineering Chiba Institute ofTechnology2-17-1TsudanumaNarashinoJapan Department of Life and Environmental Sciences Faculty of Engineering Chiba Institute ofTechnology2-17-1TsudanumaNarashinoJapan Department of Life and Environmental Sciences Faculty of Engineering Chiba Institute ofTechnology2-17-1TsudanumaNarashinoJapan

＜正＞the bee propolis is the resin material that the bee collects from a bud of particular tree or flower, the bee uses own secretion, natural material that process but become again and again through *** bee propolis paints in the exit and entrance neighborhood of the beehive of the bee, having the pest of prevent from irruptive and germs of breed the function of protect the

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