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Influence of Cell Confluency on the Expression of the α4 Integrin Subunit of Retinal Pigment Epithelial Cells

Advances in Biological Chemistry 2015年第2期5卷 73-82页

作者： Jean-Michel Bourget Mohib Morcos Karine Zaniolo sylvain L. guérin Stéphanie Proulx Centre de recherche du CHU de Québec and Centre de recherche en organogénèse expérimentale de l’Université Laval/LOEX axe médecine régénératrice Hopital du Saint-Sacrement Québec Canada Département d’anatomie et de cytologie pathologiques CHU de Québec Hopital du Saint-Sacrement Québec Canada Département de biologie moléculaire biochimie médicale et pathologie Faculté de médecine Université Laval Québec Canada Département d’Ophtalmologie Faculté de médecine Université Laval Québec Canada

Integrins are a family of transmembrane glycoproteins that mediate cell-cell and cell-extracellular matrix interactions. The integrin α4 subunit is widely expressed by cells from the immune system and its expression by non-hematopoietic cells is scarce. In the present study, gene and protein expression of this integrin subunit was characterized in proliferating and quiescent human RPE cells. Immunofluorescent studies confirm that the α4 subunit is expressed in vitro by RPE cells, a result that has been validated by immunofluorescence and FACS analyses. The accumulation of the α4 integrin at cell-cell junctions in post-confluent RPE cell cultures negatively correlated with the level of expression of the mRNA transcript. Accordingly, transient transfection analyses reveal that the α4 promoter activity is considerably reduced when RPE cells form a confluent monolayer. Moreover, transfection of recombinant constructs bearing 5’-deletions of the α4 promoter segment allows the localization of strong negative regulatory elements on the -76 to -300 region of the α4 gene suggesting that its expression is intimately linked to the proliferative state of primary cultured RPE cells.

关键词： Retinal Pigment Epithelium Integrin Alpha 4 Subunit Cell Culture Confluency Promoter

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Deciphering the mechanobiology of microglia in traumatic brain injury with advanced microsystems

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Neural Regeneration Research 2025年第8期20卷 2304-2306页

作者： Anthony Procès sylvain Gabriele Univ.Grenoble Alpes InsermU1216Grenoble Institut des NeurosciencesGrenobleFrance Mechanobiology&Biomaterials Group CIRMAPResearch Institute for BiosciencesUniversity of MonsMonsBelgium

Advanced microsystems in traumatic brain injury research:Traumatic brain injury(TBI)results from a mechanical insult to the brain,leading to neuronal and axonal damage and subsequently causing a secondary *** minutes ... 详细信息

关键词： traumatic injury damage

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*** of a new role for the WNK1 kinase in corneal wound healing

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Annals of Eye Science 2019年第1期 196-196页

作者： Pascale Desjardins Camille Couture Lucie Germain sylvain guérin Département d'ophtalmologie UniversitéLavalCUO-recherche/LOEXCentre de recherche du CHU de Québec-UniversitéLavalQuébecQCCanada Département de chirurgie UniversitéLavalCentre de recherche en organogénèse expérimentale de l’UniversitéLaval/LOEXCentre de recherche du CHU de Québec-UniversitéLavalQuébecQCCanada

Background:Damage to the corneal epithelium triggers important changes in the composition of the extracellular matrix(ECM)to which the basal human corneal epithelial cells(hCECs)*** changes are perceived by integrins,a family of trans-membrane receptors that activate different intracellular signalling pathways,ultimately leading to re-epithelialization of the injured *** goal is to study the impact of the pharmacological inhibition/activation of specific signal transduction mediators on corneal wound healing using both monolayers of hCECs and tissue-engineered human corneas(hTECs)as in vitro ***:hTECs were produced by the self-assembly approach and wounded with a 8-mm biopsy *** RNA and proteins were isolated from the wounded and unwounded hTECs to conduct gene profiling analyses and protein kinase *** wounded tissues were then incubated either with the lysine deficient protein kinase 1(WNK1)inhibitor WNK463,the WNK1 indirect agonist AM1241,or with the vehicle alone(DMSO;negative control)and wound healing was monitored for 6 *** impact of WNK1 inhibition/activation on hCECs monolayers was determined using scratch wound ***:Gene profiling analyses and protein kinases arrays revealed that expression and activity of several mediators from the integrin-dependent signalling pathways were altered in response to the ECM changes taking place during corneal wound *** of the WNK1 kinase turned out to be the most striking activation event occurring during wound *** the pharmacological inhibition of WNK1 by WNK463 significantly reduced the rate of corneal wound closure in our hTECs and hCECs monolayers compared to their respective negative controls,we believe that the pharmacological activation of WNK1 could turn out to be an interesting avenue to accelerate corneal wound ***:These results will contribute to a better understanding of the cellular and molecular mechanisms

关键词： Cornea wound healing lysine deficient protein kinase 1(WNK1)

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*** of WNK1 inhibition on corneal wound healing using a model of human tissue-engineered cornea

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Annals of Eye Science 2018年第1期 495-495页

作者： Pascale Desjardins Camille Couture Karine Zaniolo Lucie Germain sylvain guérin Département d'ophtalmologie CUO-recherche/LOEXCentre de recherche du CHU de QuébecUniversitéLavalQuébecQCCanada Département de chirurgie Centre de recherche en organogénèse expérimentale de l’UniversitéLaval/LOEXCentre de recherche du CHU de QuébecUniversitéLavalQuébecQCCanada

Background:Because of its superficial anatomical localization,the cornea is particularly vulnerable to abrasive forces and various traumas,which can lead to significant visual *** injury of the corneal epithelium,there are important changes that occur in the composition of the underlying extracellular matrix(ECM).Those changes are perceived by the integrins that recognize the ECM components as their ligand and activate different intracellular signalling pathways,ultimately leading to reepithelialisation and reorganization of the injured epithelium,both of which are necessary in order to restore the visual properties of the *** goal of this study was to analyse the impact of the pharmacological inhibition of specific signal transduction mediators of integrin-dependant signalling pathways on corneal wound healing using both monolayers of hCECs and tissue-engineered human corneas(hTECs)as in vitro ***:hTECs were produced by the self-assembly approach and wounded with a 8-mm diameter biopsy *** RNA and proteins were isolated from the wounded and unwounded hTECs to conduct gene profiling analyses and protein kinase *** wounded tissues were then incubated with the WNK1 inhibitor WNK463 and wound healing was monitored over a period of 6 *** corneas were incubated with the vehicle alone(DMSO).The impact of WNK1 inhibition on hCECs monolayers was determined using a scratch wound ***:Gene profiling analyses and protein kinases arrays revealed important alterations in the expression and activity of several mediators from the integrin-dependent signalling pathways in response to the ECM changes taking place during corneal wound *** these,WNK1 is considerably activated through phosphorylation during corneal wound *** pharmacological inhibition of WNK1 by WNK463 significantly reduced the dynamic of corneal wound closure in our hTECs and hCECs monolayers compared to their respective negative control

关键词： Cornea tissue engineering wound healing signaling pathways integrins

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*** of cyclic-AMP-response element binding protein and its impact on corneal wound healing in vitro and in vivo

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Annals of Eye Science 2019年第1期 195-195页

作者： Camille Couture Pascale Desjardins Karine Zaniolo Richard Bazin Lucie Germain sylvain guérin Département d'ophtalmologie UniversitéLavalCUO-recherche/LOEXCentre de recherche du CHU de Québec-UniversitéLavalQuébecQCCanada Département de chirurgie UniversitéLavalCentre de recherche en organogénèse expérimentale de l’UniversitéLaval/LOEXCentre de recherche du CHU de Québec-UniversitéLavalQuébecQCCanada

Background:The cornea composes the outer surface of the eye and its transparency is required to allow light transmission to the ***,because of its position,the cornea is subjected to chemical and mechanical injuries that may lead to *** studies conducted using the human tissue-engineered cornea(hTEC)as a model provided evidence that the cyclic-AMP-response element binding protein(CREB)pathway is repressed during closure of corneal *** on these results,we hypothesized that closure of corneal wounds can be enhanced by preventing activation of CREB with the pharmacological inhibitor *** goals were to proceed to the pharmacological inhibition of CREB(I)in vitro using the hTECs as a model,and then(II)in vivo using the rabbit as a ***:The self-assembly approach was used to create hTECs,that were then wounded with an 8-mm diameter biopsy punch to create an epithelial *** tissues were then incubated with 10μM of C646(n=8).DMSO was used alone as a negative control(n=4).Closure of the wounds was monitored over a period of 5 ***,the cornea of New Zealand white rabbits was debrided with an ethanol 70%solution to create an epithelial defect of 8-mm *** concentrations of C646(1,10,100μM et 1 mM)were applied as eye drops 3 times a day for up to 7 *** wounded corneas(n=4 per concentration)were stained with fluorescein and photographed every ***:In vitro pharmacological inhibition of CREB with C646 considerably accelerated wound closure of all treated hTECs(4 days)compared to the control group(7 days).Moreover,the in vivo C646 treatment also accelerated wound healing of the corneas compared to the control *** most effective concentration of C646 tested was the lowest(1μM),as it considerably enhanced the wound healing ***:This study demonstrates that wound healing both in vitro and in vivo can be enhanced by preventing activation of CREB using a pharmacological inhibition appr

关键词： Cyclic-AMP-response element binding protein(CREB) protein kinase B(AKT) healing corneal wound tissue-engineering

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*** gold nanoparticles as a drug delivery system for ocular therapy

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Annals of Eye Science 2019年第1期 198-198页

作者： Florence Masse Pascale Desjardins Mathieu Ouellette Camille Couture Mahmoud Mohamed Omar Vincent Pernet sylvain guérin Élodie Boisselier Department of Ophthalmology Laval UniversityQuebecQCCanada CUO-Recherche Hôpital du St-SacrementCentre de recherche du CHU de QuébecQuebecQCCanada Centre de Recherche en Organogénèse expérimentale de l’UniversitéLaval/LOEX QuébecQCCanada

Background:Ocular therapy administrated by ophthalmic drops is advantageous thanks to its ***,efficiency of active molecules is limited when administered by this ***,more than 99.9%is discarded due to multiple factors including lacrimal *** retention time of drugs at the cornea leads to their poor *** hypothesis is that a drug delivery system based on gold nanoparticles should enhance the efficiency of the *** main objective is to develop new methods to improve active molecules biodisponibility in ocular therapy thanks to a new drug delivery system implying gold *** specific objectives are:(I)to synthesize and characterize ultrastable gold nanoparticles,(II)to establish the drug encapsulation protocol,(III)to develop a separation method of free and encapsulated drugs to allow their quantification,(IV)to study the cytotoxicity of our gold ***:Ultrastable gold nanoparticles were synthesized by a new method and their ultrastability toward several harsh conditions was *** encapsulation protocol was settled for several *** separation of free and encapsulated drugs was performed with magnetic *** quantification of the encapsulated drugs was performed by HPLC.A MTS assay was performed on 3 corneal epithelial cell populations,exposed or unexposed to gold *** corneas were prepared using the self-assembly method.A wound healing experience was performed on those corneas with or without ***:Gold nanoparticles were synthesized and purified according to our new experimental *** support harsh conditions as several cycles of freeze-drying,heating,salt exposition and *** the first time in literature,gold nanoparticle support autoclave *** separation method involving magnetic beads was optimized to get rid of non-specific *** encapsulation efficiency varies according to the act

关键词： Gold nanoparticles ultrastability drug delivery MTS assay wound healing assay

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*** database of clinical data and biological material for research on uveal melanoma

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Annals of Eye Science 2018年第1期 453-453页

作者： Solange Landreville Frédéric Mouriaux Patrick J.Rochette Christian Salesse Mohib W.Morcos Claudine Bellerive sylvain guérin Centre de recherche du CHU de Québec-UniversitéLaval Hôpital du Saint-SacrementQuébecQCCanada Département d’ophtalmologie et d’ORL-CCF Facultéde médecineUniversitéLavalQuébecQCCanada Centre de recherche en organogénèse expérimentale de l’UniversitéLaval/LOEX QuébecQCCanada Département d'ophtalmologie Facultéde médecineUniversitéde Rennes 1RennesFrance Service d’ophtalmologie CHU de RennesRennesFrance

Background:Sharing biological material and clinical data from patients with uveal ***:Uveal melanoma is the most common intraocular malignancy in the adult *** uveal melanoma is primarily a sporadic cancer and familial cases are rare,it is difficult to prevent or detect *** effective treatment of ocular tumors,more than 50%of patients develop incurable liver metastases mainly in the 5-10 years following the detection of the primary *** cancer is relatively rare and the obtained biopsies are very *** 20 samples are taken each year in *** provincial infrastructure is made of biological material from donors with uveal melanoma and a large clinical *** tumor biopsies are used for culturing cell lines and the creation of a DNA/RNA library used for genomic and genetic ***:This infrastructure plays an important role in the achievement of various research programs for a better understanding of genetic and environmental factors involved in the development of melanoma and the spread of *** allows collaboration with other researchers at a provincial,national and international level in order to make progress in basic and clinical research on uveal ***:The biological material and clinical data of this infrastructure are available upon request to VHRN members whose research project was approved by the ethics committee of the institution.

关键词： Uveal melanoma tumor biopsies blood clinical data

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*** tissues bank for vision health research

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Annals of Eye Science 2018年第1期 499-499页

作者： Kim Santerre Mathieu Thériault sylvain Chemtob Lucie Germain Claude Giasson sylvain guérin Solange Landreville Patrick JRochette Christian Rochette Mike Sapieha Élodie Boisselier Vincent Pernet Stéphanie Proulx Centre de Recherche du CHU de Québec-UniversitéLaval Axe Médecine RégénératriceQuébecQCCanada Centre de Recherche en Organogénèse Expérimentale de l'UniversitéLaval/LOEX QuébecQCCanada Département d'Ophtalmologie et d'Oto-Rhino-Laryngologie-Chirurgie Cervico-Faciale Facultéde MédecineUniversitéLavalQuébecQCCanada Département d’ophtalmologie Universitéde MontréalQCCanada Maisonneuve-Rosemont Hospital Research Center MontrealQCCanada Département de chirurgie Facultéde MédecineUniversitéLavalQuébecQCCanada École d’optométrie Universitéde MontréalQuébecQCCanada

Background:This infrastructure delivers biological material necessary for several research projects to Vision Health Research Network investigators(VHRN).Methods:Héma-Québec is the organism in charge obtaining consent and retrieving donor eyes for patient treatment or for *** Quebec City,donor eyes are sent to the eye bank of the“Centre Universitaire d’Ophtalmologie”(CUO)of Saint-Sacrement *** at the eye bank evaluate the quality of the *** unfit for graft are transferred to the infrastructure where the coordinator encodes samples prior to their ***:Between 2013 and 2017,27 fundamental investigators,clinical investigators and collaborators supported by 60 students,trainees and laboratory assistants used this infrastructure to move forward their *** 2013,results from those projects generated 21 scientific publications and 232 *** infrastructure helped VHRN investigators obtain near 4 million dollars in grants from many organisms(CIHR,NSERC,Foundations,etc.).These grants allowed recruitment and formation of highly qualified *** year(April 2016 to March 2017),189 corneas and 23 eyes transited through the ***:This infrastructure is available for all investigators that are members of the *** original projects have been elaborated thanks to the human ocular tissues provided by this *** projects will advance our knowledge in vision health.A better understanding of eye functions will lead to new treatments for eye diseases.

关键词： Human ocular tissues for research fresh tissues

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Efficient Resource Allocation in Cloud IaaS: A Multi-Objective Strategy for Minimizing Workflow Makespan and Cloud Resource Costs

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Open Journal of Applied Sciences 2025年第1期15卷 147-167页

作者： Jean Edgard Gnimassoun Dagou Dangui Augustin sylvain Legrand Koffi Akanza Konan Ricky N’dri Dpartement Informatique et Analyse de Dones Universit de San Pedro San Pedro Cte dIvoire Dpartement Informatique Ecole Suprieure Africaine des Technologies dInformation et de Communication Abidjan Cte dIvoire Dpartement Mathmatiques et Informatique Universit Alassane Ouattara Bouak Cte dIvoire

The ease of accessing a virtually unlimited pool of resources makes Infrastructure as a Service (IaaS) clouds an ideal platform for running data-intensive workflow applications comprising hundreds of computational tasks. However, executing scientific workflows in IaaS cloud environments poses significant challenges due to conflicting objectives, such as minimizing execution time (makespan) and reducing resource utilization costs. This study responds to the increasing need for efficient and adaptable optimization solutions in dynamic and complex environments, which are critical for meeting the evolving demands of modern users and applications. This study presents an innovative multi-objective approach for scheduling scientific workflows in IaaS cloud environments. The proposed algorithm, MOS-MWMC, aims to minimize total execution time (makespan) and resource utilization costs by leveraging key features of virtual machine instances, such as a high number of cores and fast local SSD storage. By integrating realistic simulations based on the WRENCH framework, the method effectively dimensions the cloud infrastructure and optimizes resource usage. Experimental results highlight the superiority of MOS-MWMC compared to benchmark algorithms HEFT and Max-Min. The Pareto fronts obtained for the CyberShake, Epigenomics, and Montage workflows demonstrate closer proximity to the optimal front, confirming the algorithm’s ability to balance conflicting objectives. This study contributes to optimizing scientific workflows in complex environments by providing solutions tailored to specific user needs while minimizing costs and execution times.

关键词： Cloud Infrastructure Multi-Objective Scheduling Resource Cost Optimization Resource Utilization Scientific Workflows

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Morphometric Differentiation between Four Strains of Japanese Quail (Coturnix coturnix japonica) Reared in Southern Côte d’Ivoire

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Open Journal of Animal Sciences 2025年第1期15卷 77-88页

作者： Brahima Soro Isidore Kouadio Kpandji sylvain Zadi Bopo Stéphane Yves Aka André N’guessan Yao Didier Paulin Sokouri Laboratory of Biotechnology Agriculture and Valorisation of Biological Resources UFR Biosciences Flix Houphout-Boigny University Abidjan Cte dIvoire

The Japanese quail (Coturnix japonica) is a disease-resistant, high-productivity bird. It has few breeding constraints. A phenotypic characterization study of the Japanese quail was carried out in southern Côte d’Ivoire to gain a better understanding of this bird. The study involved 298 individuals. The phenotypes were white, black, gray, and red. A total of 11 morphometric parameters were examined. Variables were significantly different between the phenotype and sex groups. The female quail was larger than the male quail. The quail was 20.77 and 4.10 cm long. The head was supported by a 5.89 cm-long neck. The tail was 3.61 cm long. The wing length was 10.42 cm, and the thigh diameter was 2.12 cm. The tarsus was 3.39 cm long and 0.61 cm in diameter. The average quail weight was 297.06 g. The quails in this study exhibit a high degree of morphological variation between individuals in terms of plumage. These results can help develop strategies for developing and genetically improving this short-cycle resource.

关键词： Quail Coturnix japonica Phenotypic Characterization Improving

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