*** gold nanoparticles as a drug delivery system for ocular therapy

作     者：Florence Masse Pascale Desjardins Mathieu Ouellette Camille Couture Mahmoud Mohamed Omar Vincent Pernet Sylvain Guérin Élodie Boisselier 

作者机构：Department of OphthalmologyLaval UniversityQuebecQCCanada CUO-RechercheHôpital du St-SacrementCentre de recherche du CHU de QuébecQuebecQCCanada Centre de Recherche en Organogénèse expérimentale de l’UniversitéLaval/LOEXQuébecQCCanada 

出 版 物：《Annals of Eye Science》 (眼科学年鉴（英文）)

年 卷 期：2019年第1期

页      面：198-198页

学科分类：08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

主　　题：Gold nanoparticles ultrastability drug delivery MTS assay wound healing assay 

摘      要：Background:Ocular therapy administrated by ophthalmic drops is advantageous thanks to its ***,efficiency of active molecules is limited when administered by this ***,more than 99.9%is discarded due to multiple factors including lacrimal *** retention time of drugs at the cornea leads to their poor *** hypothesis is that a drug delivery system based on gold nanoparticles should enhance the efficiency of the *** main objective is to develop new methods to improve active molecules biodisponibility in ocular therapy thanks to a new drug delivery system implying gold *** specific objectives are:(I)to synthesize and characterize ultrastable gold nanoparticles,(II)to establish the drug encapsulation protocol,(III)to develop a separation method of free and encapsulated drugs to allow their quantification,(IV)to study the cytotoxicity of our gold ***:Ultrastable gold nanoparticles were synthesized by a new method and their ultrastability toward several harsh conditions was *** encapsulation protocol was settled for several *** separation of free and encapsulated drugs was performed with magnetic *** quantification of the encapsulated drugs was performed by HPLC.A MTS assay was performed on 3 corneal epithelial cell populations,exposed or unexposed to gold *** corneas were prepared using the self-assembly method.A wound healing experience was performed on those corneas with or without ***:Gold nanoparticles were synthesized and purified according to our new experimental *** support harsh conditions as several cycles of freeze-drying,heating,salt exposition and *** the first time in literature,gold nanoparticle support autoclave *** separation method involving magnetic beads was optimized to get rid of non-specific *** encapsulation efficiency varies according to the act