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Acylhydrazide and Isatin Schiff Bases as Alternate UV-Laser Desorption Ionization (LDI) Matrices for Low Molecular Weight (LMW) Peptides Analysis

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American Journal of Analytical Chemistry 2012年第12期3卷 779-789页

作者： syed Ghulam Musharraf Aisha Bibi Najia shahid Muhammad Najam-ul-Haq Momin Khan Muhammad Taha Uzma Rasool Mughal Khalid Mohammed Khan Department of Chemistry Bahauddin Zakariya University Multan Pakistan hussain Ebrahim Jamal Research Institute of Chemistry International Center for Chemical and Biological Sciences University of Karachi Karachi Pakistan Panjwani Center for Molecular Medicine and Drug Research International Center for Chemical and Biological Sciences University of Karachi Karachi Pakistan

Matrix-assisted laser desorption/ionization (MALDI) is a preferred and widely used mass spectrometric technique for the analysis of macromolecules. Limited UV-LDI matrices are available for the analysis of biomolecules due to the restricted structural features to serve in the laser desorption/ionization mechanism with a problem of background signals appearing in the low mass region. This paper describes the application of Schiff base derivatives of acylhydrazide and isatin as alternate UV-LDI matrices for the analysis of peptides with significantly low background signals. Thirty one compounds have been successfully employed as matrices for the analysis of low molecular weight (LMW) peptides (α-Cyano-4-hydroxycinnamic acid (HCCA), a preferred choice for peptide analysis.

关键词： MALDI-MS LDI Matrix Acylhydrazide Schiff Bases Bis-Schiff Bases of Isatin Schiff Bases of Isatin Peptides

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Multivariate Based Variability within Diverse Indian Mustard (<i>Brassica juncea</i>L.) Genotypes

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Open Journal of Genetics 2017年第2期7卷 69-83页

作者： Noor Saleem Sohail Ahmad Jan Muahmmad Jawaad Atif Haris Khurshid shahid Ali Khan Mohib Abdullah Muhammad Jahanzaib Humair Ahmed syed Farman Ullah Azhar Iqbal Sameena Naqi Muhammad Ilyas Naushad Ali Malik Ashiq Rabbani The University of Haripur KPK Pakistan Plant Genetic Resources Institute (PGRI) National Agricultural Research Centre (NARC) Islamabad Pakistan Department of Biotechnology Quaid-i-Azam University Islamabad Pakistan Oilseeds Research Program NARC Islamabad Pakistan School of Crop Production Technology Suranaree University of Technology Nakhon Ratchasima Thailand Land Resources Research Institute NARC Islamabad Pakistan Department of Plant Breeding and Genetics PMAS-Arid Agriculture University Rawalpindi Pakistan

Indian mustard (Brassica juncea L.) germplasm consisting 167 accessions including one check cultivar was evaluated for qualitative and quantitative traits. The present study was conducted to investigate genetic diversity and correlation among studied genotypes of B. juncea L. based on agro-morphological at NARC, Islamabad, Pakistan. To investigate the genetic diversity based on morphological characters, data was recorded on 20 quantitative and 12 qualitative traits. The calculated data was analyzed through two complementary methods, i.e. PCA (Principal Component Analysis) and cluster analysis. Among all the studied cultivars, significant diversity was recorded for different agro-morphological characters. Among all the parameters, maximum variance was recorded for pod shattering (427.2) followed by plant height (345.6), days to 100% flowering (336.2) and main raceme length (210.0). Among all the characters, the greatest and highly significant association (0.99) was found between days to maturity 50% and days to maturity 100% followed by correlation (0.86) among days to flowering 50% and days to flowering 100%, correlation value (0.71) was calculated among leaf length and leaf width. Using cluster analysis all the genotypes were divided into five major groups. It was observed that 7 out of 20 principal components with an Eigen value of ≥1.0 calculated for 73.92% of the total diversity observed between 167 accessions of Indian mustard (B. juncea L.). The contribution of first three PCs in the total PCs was 23.25, 12.87 and 11.24, respectively. Among all the investigated accessions two genotypes 26,813 and 26,817 showed great potential for seeds/silique, 1000-seed weight and seed yield/plant, respectively, so these genotypes are recommended for future breeding programs for achieving promising results.

关键词： Genetic Diversity Agro-Morphology Brassica juncea L. PCA Analysis

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Performance, Immune, and Carcass Characteristics of Broiler Chickens as Affected by Thyme and Licorice or Enzyme Supplemented Diets

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Open Journal of Animal Sciences 2017年第2期7卷 105-109页

作者： Majid Kalantar Seyed Mahdi Hosseini Liguo Yang Sayed Haider Abbas Raza Linsheng Gui Mostafa Rezaie Mahdi Khojastekey Dawei Wei Rajwali Khan Sulthattin Yasar shahid Faraz syed Allah Bux Kachiwal Mohamed Elkhairey Qijing Lei Rameez Raja Kaleri Ayman Hassan Abd El-Aziz Animal Science Department Agricultural Research Center of Qom Qom Iran The Key Lab of Agricultural Animal Genetics Breeding and Reproduction of the Ministry of Education College of Animal Science and Technology Huazhong Agricultural University Wuhan China College of Animal Science and Technology Northwest A&F University Yangling China Department of Animal Science Saveh Branch Islamic Azad University of Saveh Saveh Iran College of Animal Science Süleyman Demirel University Isparta Turkey College of Veterinary Medicine Northwest A&F University Yangling China Faculty of Animal Husbandry & Veterinary Sciences Sindh Agriculture University Tandojam Pakistan Faculty of Veterinary Medicine Damanhour University Damanhūr Egypt

Numbers of 300 day-old broiler chickens through a CRD design with 5 treatments, 3 replicates and 20 chicks in each pen were used to evaluate the effect of thyme (T), licorice (L), thyme + licorice (TL), and enzyme supplemented (E) diets on performance, immune and carcass characteristics. According to the results, performance traits, immune indices, and carcass traits in herbal medicine and enzyme supplemented diets were improved significantly than control diet (P < 0.05). Weight gain and FCR in T and E groups were significantly higher and lower than other groups respectively (P < 0.05). Internal organs such as abdominal fat and liver weight as indicators of lipogenesis rate were decreased in T, L, and TL diets than control or E diet significantly (P < 0.05). Immune organs such as burse and spleen weight as indicators of immune situation were increased in TL diet than other treatments significantly (P < 0.05). These findings indicated that thyme and licorice singly or in combination as organic herbal medicine can affect performance, carcass and immune characteristics. Also an improved immune organ such as burse or spleen in this study indicates that this herbal medicine can promote the immune situation and efficacy of health and livability.

关键词： Broiler Enzyme Licorice Thyme

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