Acylhydrazide and Isatin Schiff Bases as Alternate UV-Laser Desorption Ionization (LDI) Matrices for Low Molecular Weight (LMW) Peptides Analysis

作     者：Syed Ghulam Musharraf Aisha Bibi Najia Shahid Muhammad Najam-ul-Haq Momin Khan Muhammad Taha Uzma Rasool Mughal Khalid Mohammed Khan 

作者机构：Department of Chemistry Bahauddin Zakariya University Multan Pakistan Hussain Ebrahim Jamal Research Institute of Chemistry International Center for Chemical and Biological Sciences University of Karachi Karachi Pakistan Panjwani Center for Molecular Medicine and Drug Research International Center for Chemical and Biological Sciences University of Karachi Karachi Pakistan 

出 版 物：《American Journal of Analytical Chemistry》 (美国分析化学（英文）)

年 卷 期：2012年第3卷第12期

页      面：779-789页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：MALDI-MS LDI Matrix Acylhydrazide Schiff Bases Bis-Schiff Bases of Isatin Schiff Bases of Isatin Peptides 

摘      要：Matrix-assisted laser desorption/ionization (MALDI) is a preferred and widely used mass spectrometric technique for the analysis of macromolecules. Limited UV-LDI matrices are available for the analysis of biomolecules due to the restricted structural features to serve in the laser desorption/ionization mechanism with a problem of background signals appearing in the low mass region. This paper describes the application of Schiff base derivatives of acylhydrazide and isatin as alternate UV-LDI matrices for the analysis of peptides with significantly low background signals. Thirty one compounds have been successfully employed as matrices for the analysis of low molecular weight (LMW) peptides (α-Cyano-4-hydroxycinnamic acid (HCCA), a preferred choice for peptide analysis.