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Larvicidal potential of selected indigenous lichens against three mosquito species–Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi

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Chinese Herbal Medicines 2018年第2期10卷 152-156页

作者： syed zameer ahmed khader Sidhra syed zameer ahmed Kisore Perundurai Venkatesh Kamaraj Chinnaperumal Sanjeeva Nayaka Department of Biotechnology K.S.Rangasamy College of Technology Department of Biotechnology Periyar University Indian Lichenological Society Lichenology Laboratory Plant Diversity Systematics and Herbarium Division CSIR-National Botanical Research Institute (Govt. of India)

Objective：Mosquitoes are the major transmitting vectors of serious human diseases,causing millions of deaths every year with undesirable effects,including toxicity to non-target organisms.Some plants with insecticidal properties have been used in recent years for the control of a variety of pest insects and vectors.In the quest for alternative natural biological control agents against mosquito larvae lichens were selected.Method：Larvicidal activity was assessed with methanolic extracts of Parmotrema reticulatum,Parmotrema kamatti,Parmotrema tinctorum,Parmelia erumpens,Leptogium papilosum,and Roccella montagnei against Aedes aegypti,Anopheles stephensi,and Culex quinquefasciatus.The standard WHO protocols with minor modifications were adopted and the bioassay was evaluated at the concentrations of 100–500μg/m L for each lichen.Since all the lichen extracts showed complete mortality against C.quinquefasciatus in100μg/m L,the concentrations were decreased to 100,50,25,12.5,and 6.25μg/m L for C.quinquefasciatus.Larval mortality was observed for 24 h after treatment.Results：All the lichen extracts exhibited activity against third instar larvae of A.aegypti and A.stephensi at 100μg/m L,and 100%mortality was observed against the vector C.quinquefasciatus at 100μg/m L.The highest larvicidal activity was found with L.papilosum against A.aegypti（81.127μg/m L）and A.stephensi（89.10μg/m L）.Similarly,P.tinctorum and R.montagnei when tested against C.quinquefasciatus with minimum concentration〈100μg/m L exhibited significant activity withof 5.32and 6.97μg/m L.Conclusion：The bioassay results revealed larvicidal potential of lichens especially against C.quinquefasciatus with high mortality even at lower concentration.Hence,lichens can be used as an ideal sustainable approach for the control of lymphatic filariasis caused by vector C.quinquefasciatus.

关键词： larvicidal lichen mortality mosquito vectors

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Antiobesity and antihyperlipidemic effect of Ixora coccinea on Triton X-100 induced hyperlipidemia in rats: An approach to evaluate asymmetrical temperature distribution analysis using thermography

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Chinese Herbal Medicines 2019年第3期11卷 326-331页

作者： Sidhra syed zameer ahmed syed zameer ahmed khader Krishnaveni Radhakrishnan Vanmathi Marimuthu Muniraj Chinnusamy Venkatesan Thangavel Karamchand Ravi Manimaran Vetrivel Department of Biotechnology K.S.Rangasamy College of TechnologyTiruchengodeTamil Nadu 637215India Department of Electrical and Electronics Engineering K.S.Rangasamy College of TechnologyTiruchengodeTamil Nadu 637215India

Objective: Obesity and hyperlipidemia is the major cause of many pathological diseases with an increase side effects using allopathic drugs. The present study focuses on the effect of Ixora coccinea on Triton X-100 induced hyperlipidemia in rats and associated complications.Methodology: In vitro radical scavenging activity of I. coccinea was assessed using DPPH, FRAP and hydrogen peroxide. In vivo antiobesity and antihyperlipidimic activity of I. coccinea was tested in Triton X-100 induced hyperlipidemic rats and assessed for its biochemical parameters in blood and tissue samples.The relationship between physiological responses and regulation of body temperature was investigated by using animal surface temperature images captured with infrared camera.Results: The results of mineral analysis, antioxidant, total flavonoid and phenolic content represented high amount of mineral and had the potential to scavenge free radicals tested with DPPH, FRAP and hydrogen peroxide radicals with dose dependent activity. The highest activity was observed in aqueous extract, DPPH with 71.5% inhibition, FRAP with 56.8%, H2 O2 with 33% activity at 100 μg/mL concentration.Triton X-100 induced hyperlipidemic rats when treated with I. coccinea aqueous extract showed significant activity by regulating the biochemical parameters and maintaining the lipid profile by decreasing TC,LDL-C, VLDL-C, TG and improving HDL-C levels. Similarly, the elevated levels of creatinine, urea, uric acid,AST, ALT, ALP due to induction of hyperlipidemia, were brought back to near normal levels after treatment with I. coccinea. The levels of tissue anti-oxidants enzymes like SOD and CAT were also found to be improved in treated I. coccinea groups. The whole body asymmetrical temperature distribution analysis showed that significant decreases in temperature was observed in obesity induced groups but a gradual increase in temperature(2%–5%) was observed after treatment.Conclusion: Thus, the results indicated that I. coccinea can be a drug of choice to decrease

关键词： anti-oxidant hyperlipidemia Ixora coccinea Curtis obesity thermography Triton X-100

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Antihyperglycemic and antioxidant effects of Solanum xanthocarpum leaves(field grown & in vitro raised) extracts on alloxan induced diabetic rats

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Asian Pacific Journal of Tropical Medicine 2011年第10期4卷 778-785页

作者： K Poongothai P Ponmurugan K syed zameer ahmed B Senthil Kumar SA Sheriff Research and Development Centre Bharathiar UniversityCoimbatore-646041Tamil NaduIndia Department of Biotechnology K.S.Rangasamy College of TechnologyK.S.R.KalvinagarThiruchengode-637215Tamil NaduIndia Department of Biotechnology K.S.Ranga.samy College of TechnologyK.S.R.KalvinagarThiruchengode-637215Tamil NaduIndia Department of Biochemistry C.Abdul Hakeem CollegeMelvisharam-632509Tamil NaduIndia Department of Zoology Thiruvalluvar UniversitySerkaduVelloreTamil NaduIndia Department of Biochemistry College of Applied Medical SciencesShaqra UniversityShaqra-11961.Kingdom of Saudi Arabia

Objective:To investigate antidiabetic efficacy of the extract of field grown and in vitro raised leaves of Solanum xanthocarpum(S.xanthocarpum) against alloxan induced diabetic rats. Methods:The antidiabetic activity of the crude methanol extracts of the field grown and in vitro raised leaves of S.xanthocarpum at different concentrations(100-200 mg/kg bw) was tested against alloxan induced diabetic rats.The antidiabetic efficacy was validated through various biochemical parameters and the antioxidant effect was also determined.The phytochemical analyses of field grown S.xanthocarpum and in vitro rasied 5.xanthocarpum leaves were done by estimating their chlorophyll,carotenoids,total sugar,protein,amino acid and minerals contents. Results:The results revealed that the methanol extracts of both the leaves(field grown and in vitro raised) of S.xanthocarpum was efficient anti hyperglycemic agents at a concentration of 200 mg/kg bw and posses potent antioxidant activity.However,the extracts of in vitro rasied S.xanthocarpum raised leaves exhibit higher efficacy than the field grown leaves in all tested concentrations.Proximal composition and mineral analysis of S.xanthocarpum revealed higher concentration of contents in in vitro rasied S.xanthocarpum than field grown S.xanthocarpum. Conclusions:From the results it can be concluded that the leaves extracts of S.xanthocarpum can be a potential candidate in treating the hyperglycemic conditions and suits to be an agent to reduce oxidative stress.

关键词： Solanum Xanthocarpum Alloxan Field grown In vitro Oxidative stress Antioxidant Hyperglycemia Hypolipidemic

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