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Molecular mapping of leaf rust resistance genes in the wheat line Yu 356-9

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Journal of integra.ive Agriculture 2015年第7期14卷 1223-1228页

作者： HAN Liu-sha.Li Zai-feng WANG Jia-zhen SHi Ling-zhi ZHU Lin Li Xing LiU Da-qun syed J A sha. College of Plant Protection Agricultural University of Hebei Nuclear institute for Food and Agricuture

The Chinese wheat line Yu 356-9 exhibits a high level of resistance to leaf rust. in order to decipher the genetic base of resistance in Yu 356-9, gene postula.ion, inheritance analyses, and chromosome linkage mapping were carried out. Gene postula.ion completed using 15 leaf rust pathotypes and 36 isogenic lines indicated that Yu 356-9 was resistant to all pathotypes tested. F1 and F2 plants from the cross Yu 356-9 （resistant）/Zhengzhou 5389 （susceptible） were tested with leaf rust pathotype ＂FHNQ＂ in the greenhouse. Results indicated a 3：1 segrega.ion ra.io, indica.ive of the presence of a single dominant leaf rust resistance gene in Yu 356-9 which was tempora.ily designated as LrYu. Bulk segregant analysis and molecular marker assays were used to map LrYu. Five simple sequence repeat （SSR） markers on chromosome 2BS were found closely linked to LrYu. Among these markers, Xwmc770 is the most closely linked, with a genetic distance of 5.7 cM.

关键词： wheat leaf rust resistance gene inheritance analyses molecular mapping

A Novel Parallel Computing Confidentia.ity Scheme Based on Hindmarsh-Rose Model

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Computers, Materials & Continua 2023年第8期76卷 1325-1341页

作者： Jawad Ahmad Mimonah Al Qathrady Mohammed SAlshehri Yazeed Ya.in Gha.i Mujeeb Ur Rehman syed a.iz sha. School of Computing Engineering and the Built EnvironmentEdinburgh Napier UniversityEdinburghEH105DTUK Department of informa.ion Systems College of Computer Science and Information SystemsNajran UniversityNajran61441Saudi Arabia Department of Computer Science College of Computer Science and Information SystemsNajran UniversityNajran61441Saudi Arabia Department of Computer Science Al Ain UniversityAbu Dhabi112612United Arab Emirates School of Science Technology and HealthYork St John UniversityYorkYO317EXUK Research Centre for intelligent Healthcare Coventry UniversityCoventryCV15FBUK

Due to the inherent insecure nature of the internet,it is crucial to ensure the secure transmission of image data over this ***,given the limita.ions of computers,it becomes evenmore important to employ efficient and fast image encryption *** 1D chaotic maps offer a practical approach to real-time image encryption,their limited flexibility and increased vulnera.ility restrict their practical *** this research,we have utilized a 3DHindmarsh-Rosemodel to construct a secure *** randomness of the chaotic map is assessed through standard *** proposed system enhances security by incorpora.ing an increased number of system parameters and a wide range of chaotic parameters,as well as ensuring a uniformdistribution of chaotic signals across the entire value ***,a fast image encryption technique utilizing the new chaotic system is *** novelty of the approach is confirmed through time complexity *** further strengthen the resistance against cryptanalysis attacks and differential attacks,the sha.256 algorithm is employed for secure key *** results through a number of parameters demonstrate the strong cryptographic performance of the proposed image encryption approach,highlighting its exceptional suita.ility for secure ***,the security of the proposed scheme has been compared with stateof-the-art image encryption schemes,and all compa.ison metrics indicate the superior performance of the proposed scheme.

关键词： Hindmarsh-rose model image encryption sha.256 parallel computing

Assessment of Clinical Learning Environment, Supervision (CLES) among Nursing Students, Hyderabad, Sindh, Pa.istan

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Open Journal of Nursing 2019年第4期9卷 408-417页

作者： Suriya Khatoon syed Yousaf sha.Ajmal Khan Zulfiqar a.i syed a.if a.i sha.eed Mohtarma Bena.ir Bhutto Medical University Larkana Pakistan Dow University of Health Sciences Karachi Pakistan indus College of Nursing & Midwifery Karachi Pakistan Dua.institute of Nursing Qasimabad Pakistan

Background: in nursing educa.ion for better teaching and essential professional skills, the clinical practice plays a substantial role. Practice at clinical settings permits students to convert theoretic knowledge into the knowledge of the skills mandatory for the care of the pa.ient. Clinical learning environment (CLE) is an important part in educa.ion of nursing and has a sizable influence on the students’ learning. Objective: The purpose of this study is to exa.ine perception and sa.isfaction of nursing students with their CLEs in Hyderabad, Pa.istan. Methods: This cross-sectional study was conducted at three nursing institutes of Hyderabad from December 2018 to January 2019 among 342 nursing students. Clinical Learning Environment, Supervision and Nurse Teacher (CLES + T) assessment tool was used as the instrument to identify the students’ perception about the learning environment in clinical setting. Results: The mean age of the participants was 25.6 ± 4.93 with majority of them male (70.7%). Three domains, pedagogical atmosphere, supervisory rela.ionship and nurse teacher role in clinical practice showed good relia.ility of more than 70%. Highest domains vise mean score was obtained for nursing premises on the ward (3.315) whereas lowest for nurse teacher role in clinical practice (NT) (3.062). Analysis of va.iance revealed that three domains supervisory rela.ionship, leadership style of the ward manager and premises of nursing showed significant mean score difference among supervisor title. Conclusion: it was found that students valued positive supervision, ward manager leadership style premises of nursing on the ward as positive CLE. Learning environment va.ies between gender, clinical settings and supervision. Medicine ward appeared to deliver the finest learning situa.ions for the nursing program.

关键词： Clinical Placement Nursing Educa.ion Supervisor Clinical Environment

Efficacy of autologous platelet-rich plasma.in the treatment of chronic nonhea.ing leg ulcers

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Plastic and Aesthetic Research 2014年第1期1卷 65-69页

作者： Shwetha Suryanarayan Leelavathy Budamakuntla syed iqbalulla sha.Khadri Sacchidanand Sarvajnamurthy Department of Dermatology STD and LeprosyBangalore Medical College and Research InstituteBengaluru 560001KarnatakaIndia Department of General Surgery Bowring and Lady Curzon HospitalBangalore Medical College and Research InstituteBengaluru 560001KarnatakaIndia.

Aim:The aim was to evaluate the efficacy of platelet-rich plasma(PRP)in the treatment of chronic nonhea.ing ***:A nonrandomized,uncontrolled study was performed on 24 pa.ients with 33 nonhea.ing ulcers of va.ious *** pa.ients were treated with PRP at weekly intervals for a ma.imum of 6 *** the end of the 6-week period,reduction in size of the ulcers(area and volume)was ***:The mean age of the pa.ients was 42.5 years(standard devia.ion[SD]12.48).Of 33 ulcers,there were 19 venous ulcers,7 trauma.ic ulcers,2 ulcers secondary to pyoderma gangrenosum,2 diabetic ulcers,2 trophic ulcers,and 1 vasculitic *** mean dura.ion of hea.ing of the ulcers was 5.6 weeks(SD 3.23).The mean percentage of reduction in area and volume of the ulcers was 91.7%(SD 18.4%)and 95%(SD 14%),*** 100%resolution in the area was seen in 25(76%)of the ulcers and 100%reduction in volume was seen in 24(73%)of the ulcers at the end of the 6th ***:Conventional thera.ies do not provide sa.isfactory hea.ing for chronic nonhea.ing ulcers as they are not able to provide the necessary growth factors(GFs)(platelet-derived GF,epidermal GF,vascular endothelial GF,etc.)which are essential for the hea.ing *** is a safe,affordable,biocompa.ible,and simple office-based procedure for the treatment of nonhea.ing ulcers.

关键词： Leg ulcers platelet-derived growth factor platelet rich plasma

青少年多形性低级别神经上皮肿瘤临床病理学分析

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中华病理学杂志 2022年第7期51卷 640-646页

作者：赵莎王娟李明娜丁颖潘敏鸿宋坤南京医科大学第一附属医院病理科南京210029 南京医科大学附属脑科医院病理科南京210029

目的探讨青少年多形性低级别神经上皮肿瘤(polymorphous low-grade neuroepithelial tumor of the young,PLNTY)的临床病理特征、诊断及鉴别诊断要点。方法收集2019—2021年南京医科大学第一附属医院和南京医科大学附属脑科医院手术诊... 详细信息

目的探讨青少年多形性低级别神经上皮肿瘤(polymorphous low-grade neuroepithelial tumor of the young,PLNTY)的临床病理特征、诊断及鉴别诊断要点。方法收集2019—2021年南京医科大学第一附属医院和南京医科大学附属脑科医院手术诊治经病理确诊的PLNTY 5例,对其临床及影像学特点、组织学形态、免疫表型及分子遗传学改变进行分析总结,并复习文献。结果患者男性2例,女性3例;年龄10~39岁,平均年龄25岁;肿瘤位于颞叶3例,侧脑室、左尾状核各1例;临床表现癫痫3例、右眼视力下降1例,1例因外伤后体检发现。镜下观察:肿瘤特征性表现为浸润性生长、瘤细胞多形性(均可见少突胶质细胞瘤样细胞、细胞核呈低级别、多形性)及不同程度钙化。免疫组织化学:肿瘤细胞胶质纤维酸性蛋白、少突胶质细胞转录因子阳性,CD34弥漫强阳性,周围脑组织可见散在CD34阳性细胞,Ki-67阳性指数均小于3%。分子遗传学:BRAF V600E突变2例,PAK5-Q337R基因错义突变1例,FGFR2-CTNNA3基因融合1例,1例伴有FGFR2-ina和FGFR2-PPRC1基因融合。术后均未行放化疗,随访3~29个月,未见复发转移。结论PLNTY罕见,确诊依赖于病理形态及分子遗传学,认识该肿瘤的重要性在于与高级别胶质瘤鉴别,避免过度治疗,本研究新报道PAK5-Q337R错义突变和FGFR2-PPRC1基因融合,拓宽了PLNTY的分子遗传学谱系。

关键词：肿瘤神经上皮青少年分子诊断技术

含EMC6基因重组真核表达载体的构建及其在人肝L-02细胞中的异位表达

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江苏大学学报（医学版） 2017年第6期27卷 466-469页

作者： syed Hameed a.i sha. 何大伟何敖林李莎莎王炳芳江苏大学医学院江苏镇江212013 江苏大学附属昆山医院消化内科江苏昆山215300 江苏大学附属昆山医院临床实验研究中心江苏昆山215300

目的:构建pcDNA3.1(+)/EMC6真核表达载体,并将其在人肝L-02细胞株中过表达。方法:查询Pub Med上EMC6的基因序列,针对该序列设计引物,提取L-02细胞的总RNA、聚合酶链式反应(PCR)扩增目的片段,采用基因重组技术将EMC6的cDNA定向插入真核... 详细信息

目的:构建pcDNA3.1(+)/EMC6真核表达载体,并将其在人肝L-02细胞株中过表达。方法:查询Pub Med上EMC6的基因序列,针对该序列设计引物,提取L-02细胞的总RNA、聚合酶链式反应(PCR)扩增目的片段,采用基因重组技术将EMC6的cDNA定向插入真核表达载体pcDNA3.1中,构建pcDNA3.1(+)/EMC6真核表达载体,经菌落PCR,测序验证构建的准确性。应用脂质体转染方法将重组载体转入L-02细胞中,RT-PCR、蛋白质印迹法检测EMC6基因的mRNA和蛋白表达水平。结果:PCR和基因测序结果均表明pcDNA3.1(+)/EMC6重组质粒构建成功,RT-PCR和蛋白质印迹检测结果证实EMC6在人肝L-02细胞中过表达。结论:成功构建人pcDNA3.1(+)/EMC6重组质粒,并在L-02细胞中过表达。

关键词：内质网膜蛋白复合体亚基-6 重组质粒载体构建转染 L-02细胞

高校微信公众号用户满意度影响因子研究——以华中师范大学为例

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大学图书情报学刊 2016年第6期34卷 109-116页

作者：冯莎莎华中师范大学信息管理学院武汉430079

研究高校微信公众号订阅用户数据,分析用户满意度影响因子,为改善用户体验,提高活跃度提供参考。构建一个综合模型,以高校学生为主要调查对象,采用结构方程模型对其影响因素进行量化分析。调查研究表明,高校微信公众号满意度影响因子有... 详细信息

研究高校微信公众号订阅用户数据,分析用户满意度影响因子,为改善用户体验,提高活跃度提供参考。构建一个综合模型,以高校学生为主要调查对象,采用结构方程模型对其影响因素进行量化分析。调查研究表明,高校微信公众号满意度影响因子有:感知有用性、感知易用性、感知娱乐性、信息感知质量性和高校微信公众号使用意愿。同时,高校微信公众号的使用意愿主要受到感知有用性、感知娱乐性与信息感知质量性三个因素的影响,而信息感知质量性发挥中介作用,联系和调节各种因素之间的关系。

关键词：高校微信公众号 TAM模型结构方程模型用户满意度用户服务用户体验

妊娠期高血压患者外周血miR-210表达与UACR、MDA、SOD、T-AOC、TNF-α及iL-6的关系

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湖南师范大学学报（医学版） 2022年第4期19卷 94-97页

作者：孙莎高志敏武丽蕊袁克芳李红霞河北中石油中心医院廊坊065000

目的:探索外周血miR-210与妊娠期高血压患者表达水平及临床指标的相关性。方法:取我院 2020年7月~2022年3月138例妊娠期高血压疾病患者作为研究对象,作为研究组,据病情程度分为三组:妊娠期高血压亚组(n=34,A组)、轻度子痫前期亚组(n=79,... 详细信息

目的:探索外周血miR-210与妊娠期高血压患者表达水平及临床指标的相关性。方法:取我院 2020年7月~2022年3月138例妊娠期高血压疾病患者作为研究对象,作为研究组,据病情程度分为三组:妊娠期高血压亚组(n=34,A组)、轻度子痫前期亚组(n=79,B组)、重度子痫前期亚组(n=25,C组),取50例单胎健康孕妇为对照组。比较两组血清 miR-210 表达水平及血清炎性因子如白细胞介素6(iL-6)、肿瘤坏死因子-α(TNF-α),氧化应激指标如丙二醛(MDA)、超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)及尿微量白蛋白/肌酐比值(UACR),并分析血清miR-210 与UACR、MDA、SOD、T-AOC、TNF-α及iL-6的相关性。结果:研究组患者miR-210、iL-6、TNF-α均高于对照组;研究组患者SOD、T-AOC水平均低于对照组,UACR、MAD水平高于对照组;B组miR-210、iL-6、TNF-α水平高于A组,且C组miR-210、iL-6、TNF-α水平高于B组;SOD、T-AOC水平在A组、B组、C组中依次降低,UACR、MAD水平在A组、B组、C组中依次升高;A组清miR-210 与 TNF-α、iL-6、MAD、UACR呈正相关,与 SOD、T-AOC呈负相关。结论:miR-210 通过介导炎性反应、氧化应激、血管内皮细胞的损伤过程而参与 HDCP 疾病发生发展,可作为临床诊断HDCP 的生物标志物。

关键词：白细胞介素6 肿瘤坏死因子-α 丙二醛妊娠期高血压

islet-1促C3H10T1/2细胞向心肌样细胞分化过程中的电生理特性

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临床心血管病杂志 2015年第10期31卷 1100-1105页

作者：杨春梅许皓易勤燕莎崔建邦田杰朱静重庆医科大学儿科研究所儿童发育与疾病研究教育部重点实验室儿科学重庆市重点实验室重庆400014 重庆医科大学附属儿童医院心血管内科

目的:研究islet-1体外诱导C3H10T1/2细胞分化为心肌样细胞过程中,钠、钙离子通道电流的表达情况。方法:islet-1慢病毒载体感染C3H10T1/2细胞,观察细胞形态变化。差速贴壁法分离培养新生小鼠心肌细胞,以免疫荧光法进行鉴定。RT-PCR检测... 详细信息

目的:研究islet-1体外诱导C3H10T1/2细胞分化为心肌样细胞过程中,钠、钙离子通道电流的表达情况。方法:islet-1慢病毒载体感染C3H10T1/2细胞,观察细胞形态变化。差速贴壁法分离培养新生小鼠心肌细胞,以免疫荧光法进行鉴定。RT-PCR检测相应钠、钙离子通道基因mRNA表达水平。全细胞膜片钳技术检测细胞钠、钙离子通道电流表达情况。结果:实验组细胞形态呈心肌样改变;实验组钠、钙离子通道基因SCN5A(ina离子通道基因)、CACNA1C(iL-Ca离子通道基因)和CACNA1H(iT-Ca离子通道基因)表达量均明显高于空白组和阴性对照组(均Pina)和T型钙电流(iT-Ca),峰值电流呈现不均一性,总体水平低于小鼠心肌细胞;iL-Ca的离子通道基因虽表达增高,但未测得电流表达。结论:实验组钠、钙离子通道基因表达增高,可产生与心肌细胞相似的钠、钙离子通道电流,说明islet-1可诱导C3H10T1/2细胞分化为有电生理功能的心肌样细胞。

关键词： islet-1 间充质干细胞心肌细胞离子通道电流