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In vitro interference of cefotaxime at subinhibitory concentrations on biofilm formation by nontypeable Haemophilus influenzae

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Asian Pacific Journal of Tropical Biomedicine 2016年第9期6卷 745-750页

作者： Sudarat Baothong sutthirat sitthisak Duangkamol Kunthalert Department of Microbiology and Parasitology Faculty of Medical ScienceNaresuan University Centre of Excellence in Medical Biotechnology Faculty of Medical ScienceNaresuan University

Objective:To investigate the in vitro interference of cefotaxime at subinhibitory concentrations [sub-minimal inhibitory concentrations(MIC)] on biofilm formation by nontypeable Haemophilus influenzae(NTHi).Methods:The interference of subinhibitory concentrations of cefotaxime on biofilm formation of the clinical strong-biofilm forming isolates of NTHi was evaluated by a microtiter plate biofilm formation *** effect of sub-MIC cefotaxime on bacterial cell-surface hydrophobicity was determined using a standard microbial adhesion to n-hexadecane ***,the effects on bacterial adherence to human fibronectin and expression of bacterial adhesins were also ***:Subinhibitory concentrations of cefotaxime,both at 0.1× and 0.5× MIC levels,efficiently reduced the NTHi biofilm formation,and this effect was independent of decreasing bacterial ***-MIC cefotaxime also decreased bacterial cell-surface hydrophobicity and reduced adherence to human *** in the P2 and P6 gene expressions upon exposure to sub-MIC cefotaxime was also ***:Taken together,our results indicate that sub-MIC cefotaxime interferes with the formation of NTHi biofilm,and this effect is feasibly related to the interference with cell-surface hydrophobicity,fibronectin-binding activity as well as alteration of the P2 and P6 gene *** findings of the present study therefore provide a rationale for the use of subinhibitory concentrations of cefotaxime for treatment of NTHi-related diseases.

关键词： Biofilm formation Cefotaxime Nontypeable Haemophilus influenzae Subinhibitory concentration Sub-MIC

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Emergence of staphylococcal cassette chromosome mec type Ⅰ with high-level mupirocin resistance among methicillin-resistant Staphylococcus aureus

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Asian Pacific Journal of Tropical Biomedicine 2017年第3期7卷 193-197页

作者： Prabhu Raj Joshi Mahesh Acharya Rajan Aryal Kamal Thapa Trishna Kakshapati Rathanin Seng Anjana Singh sutthirat sitthisak Central Department of Microbiology Tribhuvan UniversityKirtipurKathmanduNepal Department of Microbiology Kantipur College of Medical ScienceTribhuvan UniversitySitapailaKathmanduNepal Department of Microbiology Kathmandu College of Science and TechnologyTribhuvan UniversityKalimatiKathmanduNepal Department of Pathology Annapurna Neurological Institute and Allied SciencesMaitigharKathmanduNepal Department of Microbiology and Parasitology Faculty of Medical SciencesNaresuan UniversityPhitsanulokThailand Centre of Excellence in Medical Biotechnology Faculty of Medical ScienceNaresuan UniversityPhitsanulokThailand

Objective: To investigate the molecular epidemiology and antimicrobial resistance patterns of methicillin-resistant Staphylococcus aureus(MRSA) among healthcare workers and ***: MRSA isolates were recovered from nasal swabs collected at a tertiary care hospital of Nepal and confirmed on the basis of Gram staining, conventional biochemical tests, and PCR amplification of mec A gene. PCRs were also used for detection of the different resistance genes and staphylococcal cassette chromosome(SCC) mec *** susceptibility patterns of isolates were assessed by disc diffusion method and minimum inhibitory concentrations were determined by ***: A total of 29 MRSA were isolated from 536 nasal swabs(5.4%) of health care workers and patients at a tertiary care hospital in Nepal. All isolates were susceptible to amikacin, gentamicin, vancomycin(minimal inhibitory concentrations 1 024 mg/m L).Fourteen isolates were found harboring the mup A gene and one isolate was found carrying the novel mup B gene. High prevalence(68%) of SCCmec I type was found, followed by SCCmec V(13%) and SCCmec III(3%) among all the MRSA ***: We found the emergence of SCCmec type I with high-level mupirocin resistance among MRSA in Nepal. Data also suggest that MRSA SCCmec type V strain has spread from the community to the hospital.

关键词： Methicillin-resistant Staphylococcus aureus Staphylococcal cassette chromosome mec types Mupirocin resistance Nasal carriage

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