Emergence of staphylococcal cassette chromosome mec type Ⅰ with high-level mupirocin resistance among methicillin-resistant Staphylococcus aureus

作     者：Prabhu Raj Joshi Mahesh Acharya Rajan Aryal Kamal Thapa Trishna Kakshapati Rathanin Seng Anjana Singh Sutthirat Sitthisak 

作者机构：Central Department of MicrobiologyTribhuvan UniversityKirtipurKathmanduNepal Department of MicrobiologyKantipur College of Medical ScienceTribhuvan UniversitySitapailaKathmanduNepal Department of MicrobiologyKathmandu College of Science and TechnologyTribhuvan UniversityKalimatiKathmanduNepal Department of PathologyAnnapurna Neurological Institute and Allied SciencesMaitigharKathmanduNepal Department of Microbiology and ParasitologyFaculty of Medical SciencesNaresuan UniversityPhitsanulokThailand Centre of Excellence in Medical BiotechnologyFaculty of Medical ScienceNaresuan UniversityPhitsanulokThailand 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2017年第7卷第3期

页      面：193-197页

核心收录：

学科分类：1010[医学-医学技术（可授医学、理学学位）] 10[医学] 

基　　金：Supported by Central Department of Microbiology Tribhuvan University and Annapurna Neurological Institute and Allied Sciences and supported from National Research Council of Thailand 2016(R2560B064) 

主　　题：Methicillin-resistant Staphylococcus aureus Staphylococcal cassette chromosome mec types Mupirocin resistance Nasal carriage 

摘      要：Objective: To investigate the molecular epidemiology and antimicrobial resistance patterns of methicillin-resistant Staphylococcus aureus(MRSA) among healthcare workers and ***: MRSA isolates were recovered from nasal swabs collected at a tertiary care hospital of Nepal and confirmed on the basis of Gram staining, conventional biochemical tests, and PCR amplification of mec A gene. PCRs were also used for detection of the different resistance genes and staphylococcal cassette chromosome(SCC) mec *** susceptibility patterns of isolates were assessed by disc diffusion method and minimum inhibitory concentrations were determined by ***: A total of 29 MRSA were isolated from 536 nasal swabs(5.4%) of health care workers and patients at a tertiary care hospital in Nepal. All isolates were susceptible to amikacin, gentamicin, vancomycin(minimal inhibitory concentrations 1 024 mg/m L).Fourteen isolates were found harboring the mup A gene and one isolate was found carrying the novel mup B gene. High prevalence(68%) of SCCmec I type was found, followed by SCCmec V(13%) and SCCmec III(3%) among all the MRSA ***: We found the emergence of SCCmec type I with high-level mupirocin resistance among MRSA in Nepal. Data also suggest that MRSA SCCmec type V strain has spread from the community to the hospital.