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Structured PDMS Chambers for Enhanced Human Neuronal Cell Activity on MEA Platforms

Journal of Bionic Engineering 2012年第1期9卷 1-10页

作者： Joose Kreutzer Laura Yla-Outinen Paula Karna Tiina Kaarela Jarno Mikkonen Heli Skottman susanna narkilahti Pasi KaUio Department of Automation Science and Engineering Tampere University of Technology Korkeakoulunkatu 3 33720 Tampere Finland BioMediTech Biokatu 10 33520 Tampere Finland NeuroGroup Institute of Biomedical Technology University of Tampere Biokatu 12 33520 Tampere Finland The Science Center of Pirkanmaa Hospital District Biokatu 12 33521 Tampere Finland Department of Psychology University of Jyvdskyld Ylistonmgientie 33 40500 Jyvdskyla Finland

Structured poly（dimethylsiloxane）（PDMS） chambers were designed and fabricated to enhance the signaling of human Embryonic Stem Cell （hESC） - derived neuronal networks on Microelectrode Array （MEA） platforms. The structured PDMS chambers enable cell seeding on restricted areas and thus, reduce the amount of needed coating materials and cells. In addition, the neuronal cells formed spontaneously active networks faster in the structured PDMS chambers than that in control chambers. In the PDMS chambers, the neuronal networks were more active and able to develop their signaling into organized signal trains faster than control cultures. The PDMS chamber design enables much more repeatable analysis and rapid growth of functional neuronal network in vitro. Moreover, due to its easy and cheap fabrication process, new configurations can be easily fabricated based on investigator requirements.

关键词： cell culturing electrical activity human embryonic stem cells microelectrode array poly(dimethylsiloxane)

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Carbon nanotube micropillars trigger guided growth of complex human neural stem cells networks

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Nano Research 2019年第11期12卷 2894-2899页

作者： Gabriela S.Lorite Laura Yla-Outinen Lauriane Janssen Olli Pitkanen Tiina Joki Janne T.Koivisto Minna Kellomaki Robert Vajtai susanna narkilahti Krisztian Kordas Microelectronics Research Unit University of OuluOuluPO BOX 4500FI 90014Finland NeuroGroup BioMediTech and Faculty of Medicine and Health technologyTampere UniversityArvo Ylpön katu 34TampereFI 33520Finland Biomaterials&Tissue Engineering Group BioMediTechFaculty of Medicine and Health TechnologyTampere UniversityPO Box 527TampereFI 33101Finland Department of Materials Science and Nanoengineering Rice UniversityP.O.Box 1892HoustonTX77251-1892USA

New strategies for spatially controlled growth of human neurons may provide viable solutions to treat and recover peripheral or spinal cord *** topography cues are known to promote attachment and direct proliferation of many cell types,guided outgrowth of human neurites has been found difficult to achieve so ***,three-dimensional(3D)micropatterned carbon nanotube(CNT)templates are used to effectively direct human neurite stem cell *** exploiting the mechanical flexibility,electrically conductivity and texture of the 3D CNT micropillars,a perfect environment is created to achieve specific guidance of human neurites,which may lead to enhanced therapeutic effects within the injured spinal cord or peripheral *** is found that the 3D CNT micropillars grant excellent anchoring for adjacent neurites to form seamless neuronal networks that can be grown to any arbitrary shape and *** from clear practical relevance in regenerative medicine,these results using the CNT based templates on Si chips also can pave the road for new types of microelectrode arrays to study cell network electrophysiology.

关键词： carbon nanotubes multiple cues guided neurite outgrowth human neural stem cells neuronal networks

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Human dental pulp stem cells differentiate into neural precursors but not into mature functional neurons

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Stem Cell Discovery 2012年第3期2卷 85-91页

作者： Riikka Aanismaa Jenna Hautala Annukka Vuorinen susanna Miettinen susanna narkilahti Adult Stem Cell Group Institute of Biomedical Technology University of Tampere Tampere Finland Neuro Group Institute of Biomedical Technology University of Tampere Tampere Finland

Large numbers of neuronal cells are needed for regenerative medicine to treat patients suffering from central nervous system diseases and deficits such as Parkinson’s disease and spinal cord injury. One suggestion has been the utilization of human dental pulp stem cells (hDPSCs) for production of neuronal cells which would offer a patient-specific cell source for these treatments. Neuronal differentiation of hDPSCs has been described previously. Here, we tested the differentiation of DPSCs into neuronal cells with previously reported protocol and characterized the cells according to their morphology, gene and protein expressions and most importantly according to their spontaneous electrical functionality with microelectrode array platform (MEA). Our results showed that even though hDPSC-derived neural progenitor stage cells could be produced, these cells did not mature further into functional neuronal cells. Thus, utilization of DPSCs as a cell source for producing grafts to treat neurological deficits requires more efforts before being optimal.

关键词： Dental Pulp Stem Cell Neural Differentiation Neural Networks

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