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Root-associated(rhizosphere and endosphere) microbiomes of the Miscanthus sinensis and their response to the heavy metal contamination

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Journal of Environmental Sciences 2021年第6期33卷 387-398页

作者： xiaoxu sun Benru Song Rui Xu Miaomiao Zhang Pin Gao Hanzhi Lin Weimin sun National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and ManagementInstitute of Eco-environmental and Soil SciencesGuangdong Academy of SciencesGuangzhou 510650China

The plant root-associated microbiomes, including both the rhizosphere and the root endosphere microbial community, are considered as a critical extension of the plant genome. Comparing to the well-studied rhizosphere microbiome, the understanding of the root endophytic microbiome is still in its infancy. Miscanthus sinensis is a pioneering plant that could thrive on metal contaminated lands and holds the potential for phytoremediation applications. Characterizing its root-associated microbiome, especially the root endophytic microbiome, could provide pivotal knowledge for phytoremediation of mine tailings. In the current study, M. sinensis residing in two Pb/Zn tailings and one uncontaminated site were collected. The results demonstrated that the metal contaminant fractions exposed strong impacts on the microbial community structures. Their influences on the microbial community, however, gradually decreases from the bulk soil through the rhizosphere soil and finally to the endosphere, which resulting in distinct root endophytic microbial community structures compared to both the bulk and rhizosphere soil. Diverse members affiliated with the order Rhizobiales was identified as the core microbiome residing in the root of M. sinensis. In addition, enrichment of plant-growth promoting functions within the root endosphere were predicted, suggesting the root endophytes may provide critical services to the host plant. The current study provides new insights into taxonomy and potential functions of the root-associated microbiomes of the pioneer plant, M. sinensis, which may facilitate future phytoremediation practices.

关键词： Tailing Miscanthus sinensis Root endosphere Metal(loid)s contamination

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Stable isotopes applied as water tracers for infiltration experiment

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Nuclear Science and Techniques 2011年第4期22卷 251-256页

作者： LIU Xiaoyan CHEN Jiansheng sun xiaoxu SU Zhiguo State Key Laboratory of Hydrology Water Resources & Hydraulic Engineering Hohai University Nanjing 210098 China College of Earth Sciences and Engineering Hohai University Nanjing 210098 China

The δD and δ18O vertical profiles of soil water were measured prior to and after a rainfall *** of soil water movement were deciphered by comparing the soil water isotope profiles with the isotopic composition of *** results show that evaporation at the upper depth led to enrichment of the heavy *** to the loess profile,the shallow soil water of sand profile is relatively enriched in D and 18O due to macro-pore and low water-holding *** precipitation is infiltrated into soil in piston mode,accompanied with significant mixing of older soil *** preferential fluid flow in loess was observed at depths of 0-20 cm,caused by cracks in the *** hydrogen and oxygen isotopic compositions in outflow are close to the precipitation,which shows a mixing of the precipitation and old soil water,and indicates that the isotopic composition of outflow water is mainly controlled by that of the *** δD and δ18O in outflow decreased with time until stable δ values of outflow are close to those of the precipitation.

关键词：土壤水分测定稳定同位素渗透实验同位素组成应用示踪垂直剖面黄土剖面

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Chemical and Isotopic Approach to Groundwater Cycle in Western Qaidam Basin,China

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Chinese Geographical Science 2009年第4期19卷 357-364页

作者： TAN Hongbing RAO Wenbo CHEN Jiansheng SU Zhiguo sun xiaoxu LIU Xiaoyan State Key Laboratory of Hydrology- Water Resources and Hydraulic Engineering Hohai University Nanjing 210098 China Department of Earth Science and Engineering College of Civil Engineering Hohai University Nanjing 210098 China

Due to the extremely arid climate in the western Qaidam Basin,the groundwater almost becomes the single water source for local residents and industrial *** is necessary to know the reliable information on the groundwater cycle in this region for reasonable and sustainable exploitation of the groundwater resources with the further execution of recycling economy *** study focused on the recharge,the flow rate and the discharge of groundwater in the western Qaidam Basin through investigations on water chemistry and ***,chemical and isotopic characteristics show that the groundwater in the western Qaidam Basin was recharged by meltwater from new surface snow and old bottom glaciers on the northern slope of the Kunlun *** addition,the results also prove that the source water is enough and stable,and the rates of the circulation and renewal of the groundwater are relatively ***,it can be concluded that the groundwater resources would guarantee the regional requirement if the meltwater volume of the mountains has not a great changes in future,moreover,water exploitation should be limited to the renewable amount of the groundwater reservoir in the western Qaidam Basin.

关键词： groundwater cycle water chemistry isotope Qaidam Basin

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Stable Isotopes and Chloride Applied as Soil Water Tracers for Phreatic Evaporation Experiment

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Journal of Harbin Institute of Technology(New Series) 2018年第3期25卷 88-96页

作者： xiaoxu sun Jin Xu Jiansheng Chen School of Environmental Engineering Nanjing Institute of Technology School of Earth Sciences and Engineering Hohai University

A phreatic water evaporation experiment,without rainfall influence,was designed to study the mechanisms of soil water movement through groundwater recharge to the unsaturated zone. Soil moisture content,chloride concentration,and δD and δ~18 O values of soil water were measured. Results showthat with decreasing soil moisture content,the chloride concentration of leachate( ρ_f(Cl)) in the capillary water layer decreases,whereas the ρ_f(Cl) value of the hanging and film water layers above the capillary water layer increases. With the combined δD and δ~18 O values,the soil water in the hanging and film water layers is influenced by evaporation,although a dry sand layer of 39 cm exists above the wet sand layer. The highest evaporation rate and the largest salt accumulation occur at a depth of about 39 cm in columns d,e,and f(Six polyvinyl chloride columns were assigned as column a,b,c,d,e,and f). We deduce that soil water migrates in the form of liquid water above the capillary water layer. In the experiment,a part of phreatic water consumed is used for the movement of soil water,whereas the other part is lost to evaporation. Soil water could continue migrating upward with prolonged experiment duration.

关键词： soil water chloride stable isotope film water phreatic water evaporation

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Study on the mechanism of isotope fractionation in soil water during the evaporation process under equilibrium condition

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Chinese Journal Of Geochemistry 2009年第4期28卷 351-357页

作者： sun xiaoxu CHEN Jiansheng TAN Hongbing RAO Wenbo WANG Yongsen LIU Xiaoyan SU Zhiguo State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering Hohai University Nanjing 210098 China College of Water Conservancy and Hydropower Engineering Hohai University Nanjing 210098 China Reserch Institute of Science and Technology Hohai University Nanjing 210098 China

In this study, with the method of vacuum extraction, two evaporative processes of soil water and free water under equilibrium condition were simulated. For each sample,water vapor was condensed by liquid nitrogen and was collected in four time intervals. From the analysis of hydrogen and oxygen isotopic compositions of the water collected at different times, it was discovered that the isotope fractionation of soil water also follows the mode, which is just the same as the evaporative process of free water. The relationship between the stable hydrogen and oxygen isotopes in residual water showed that the simulative evaporation line was close to the global meteoric water line (GMWL) under the equilibrium condition at about 20℃. Comparison of the two types of evaporative processes indicated that the isotope fractionation and evaporation velocity of soil water were only slightly modified by the Van der Waals force.

关键词：氧同位素分析土壤水分平衡条件工艺条件水蒸发分馏机理同位素分馏水汽凝结

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Novel adaptive Hatch filter to mitigate the effects of ionosphere and multipath on LAAS

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Journal of Systems Engineering and Electronics 2010年第6期21卷 1046-1053页

作者： Lin Zhao, Liang Li, Ming sun, and xiaoxu Wang College of Automation, Harbin Engineering University, Harbin 150001, P. R. China College of Automation Harbin Engineering University Harbin 150001 P. R. China

It has been proven that carrier smoothing and differential global positioning system （DGPS） are effective to improve the accuracy of pseudorange by reducing the noise in it and eliminating almost all the common mode errors between the ground station and user. However, another issue coming with local area augmentation system （LAAS） is how to find an adaptive smoothing window width to minimize the error on account of ionosphere delay and multipath. Based on the errors analysis in carrier smoothing process, a novel algorithm is formulated to design adaptive Hatch filter whose smoothing window width flexibly varies with the characteristic of ionosphere delay and multipath in the differential carrier smoothing process. By conducting the simulation in LAAS and after compared with traditional Hatch filers, it reveals that not only the accuracy of differential correction, but also the accuracy and the robustness of positioning results are significantly improved by using the designed adaptive Hatch filter.

关键词： Hatch filter adaptive ionosphere delay multipath differential correction local area augmentation system.

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1T′‐MoTe_(2) monolayer:A promising two‐dimensional catalyst for the electrochemical production of hydrogen peroxide

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Chinese Journal of Catalysis 2022年第6期43卷 1520-1526页

作者： xiaoxu sun Xiaorong Zhu Yu Wang Yafei Li Jiangsu Collaborative Innovation Centre of Biomedical Functional Materials Jiangsu Key Laboratory of New Power BatteriesSchool of Chemistry and Materials ScienceNanjing Normal UniversityNanjing 210023JiangsuChina

The direct synthesis of hydrogen peroxide(H_(2)O_(2))via a two‐electron oxygen reduction reaction(2e‐ORR)in acidic media has emerged as a green process for the production of this valuable ***,such an approach employs expensive noble‐metal‐based electrocatalysts,which severely undermines its feasibility when implemented on an industrial ***,based on density functional theory computations and microkinetic modeling,we demonstrate that a novel two‐dimensional(2D)material,namely a 1T′‐MoTe_(2)monolayer,can serve as an efficient non‐precious electrocatalyst to facilitate the 2e‐*** 1T′‐MoTe_(2)monolayer is a stable 2D crystal that can be easily produced through exfoliation *** surface‐exposed Te sites of the 1T′‐MoTe_(2)monolayer exhibit a favorable OOH*binding energy of 4.24 eV,resulting in a rather high basal plane activity toward the 2e‐***,kinetic computations indicate that the 1T'‐MoTe_(2)monolayer preferentially promotes the formation of H_(2)O_(2)over the competing four‐electron ORR *** desirable characteristics render 1T′‐MoTe_(2)a promising candidate for catalyzing the electrochemical reduction of O_(2)to H_(2)O_(2).

关键词： 1T′‐MoTe_(2) Two‐dimensional catalyst Electrochemical H_(2)O_(2) production Density functional theory computations Microkinetic modeling

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Nitrogen fixation process and it's ecological role in mine tailings

Nitrogen fixation process and it's ecological role in mine t...

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第十届重金属污染防治技术及风险评价研讨会

作者： xiaoxu sun Tianle Kong Max Kolton Weimin sun Institute of Eco-environmental and Soil Sciences Guangdong Academy of Sciences

Nutrient deficiency,especially bioavailable nitrogen deficiency,often impedes the bioremediation efforts of mining generated *** nitrogen-fixation is a critical process necessary for the initial nitrogen buildup in *** knowledge regarding the diazotrophs that inhabit tailings is still in its ***,in this study,a comprehensive investigation combining geochemical characterization,sequence analyses,molecular techniques,and activity measurements was conducted to characterize the diazotrophic community residing in tailing *** differences between tailings and their adjacent soils in prokaryotic and diazotrophic communities were ***,strong and significant correlations between the absolute abundance of the nitrogen fixation(nif H),carbon fixation(cbb L),sulfur oxidation(sox B),and arsenite oxidation(aio A) genes were observed in the tailings,but not in the *** reconstructed nif-containing metagenome-assembled genomes(MAGs) suggest that the carbon fixation and sulfur oxidation pathways were important for potential diazotrophs inhabiting the *** measurements further confirmed that diazotrophs inhabiting tailings preferentially to use the inorganic electron donors(e.g.,elemental sulfur) compared to organic electron donors(e.g.,sucrose),while diazotrophs inhabiting soils preferred organic carbon ***,these findings suggest that chemolithoautotrophic diazotrophs may play essential roles in acquiring nutrients and facilitating ecological succession in tailings.

关键词： Mine tailings Nitrogen fixation Metabolism

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Analysis of Gravity Changes before and after the Akto MS6.7 Earthquake in 2016

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Earthquake Research in China 2018年第3期32卷 377-387页

作者： Ailixiati Yushan Liu Daiqin Li Jie Li Rui Abudutayier Yasen sun xiaoxu Zhu Zhiguo Li Guirong Chen Li Earthquake Agency of Xinjiang Uygur Autonomous Region Urumqi 830011China

This paper analyzes the characteristics of time sequence changes of gravity points near the epicenter,different changes of measuring lines and gravity changes of measuring areas in point-line-area manner respectively with the 5-period mobile gravity data through densified observation by the South Xinjiang Observation Network after the 2015-2016 Akto earthquake in Xinjiang. The gravity observation results before the earthquake indicate that the Wuqia-Bulungkol area near the epicenter presented the trend of gravity value increasing since 2015,but the gravity value decreased half a year before the earthquake,and witnessed a high gradient zone of gravity changes during some periods before the earthquake. The gravity observation results after the earthquake show that there is a trend of opposite changes in gravity difference on the northern and southern sides of Bulunkou,and good correspondence exists between the characteristics of gravity field changes near the epicenter before and after the earthquake and the geologic structure distribution in the area.

关键词： Akto earthquake Gravity changes Gravity anomalies

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GJM型棒式搅拌磨机工业试验研究

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有色金属（选矿部分） 2017年第3期 66-69页

作者：孙小旭北矿机电科技有限责任公司北京100160 北京矿冶研究总院北京100160

在介绍石墨资源储量分布和鳞片石墨选矿流程的基础上,描述了GJM型棒式搅拌磨机的主要结构和工作原理,详细叙述了磨机的空载试验、条件探索试验和连续带矿试验。试验过程中,考察了磨机给料、排料粒度,测定了精选3浮选精矿和精选4浮选精... 详细信息

在介绍石墨资源储量分布和鳞片石墨选矿流程的基础上,描述了GJM型棒式搅拌磨机的主要结构和工作原理,详细叙述了磨机的空载试验、条件探索试验和连续带矿试验。试验过程中,考察了磨机给料、排料粒度,测定了精选3浮选精矿和精选4浮选精矿品位。条件探索试验结果表明,磨机电机最佳运行频率为40 Hz、充填率为30%;连续带矿试验结果表明,相同工况下,经GJM型棒式搅拌磨机再磨后,浮选品位提升幅度比原有设备高40%以上,研磨介质消耗仅为原来的50%,设备节能25%以上。除此之外,设备整体运行平稳,可实现24 h以上带矿带介质满负荷启动,达到设计要求。总之,对于鳞片石墨再磨而言,GJM型棒式搅拌磨机是一款更为理想的设备。

关键词：棒式搅拌磨机鳞片石墨再磨工业试验

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