Nitrogen fixation process and it's ecological role in mine tailings
作者单位：Institute of Eco-environmental and Soil SciencesGuangdong Academy of Sciences
会议名称：《第十届重金属污染防治技术及风险评价研讨会》
会议日期：2020年
学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 09[农学] 0903[农学-农业资源与环境] 090302[农学-植物营养学]
关 键 词：Mine tailings Nitrogen fixation Metabolism
摘 要：Nutrient deficiency,especially bioavailable nitrogen deficiency,often impedes the bioremediation efforts of mining generated *** nitrogen-fixation is a critical process necessary for the initial nitrogen buildup in *** knowledge regarding the diazotrophs that inhabit tailings is still in its ***,in this study,a comprehensive investigation combining geochemical characterization,sequence analyses,molecular techniques,and activity measurements was conducted to characterize the diazotrophic community residing in tailing *** differences between tailings and their adjacent soils in prokaryotic and diazotrophic communities were ***,strong and significant correlations between the absolute abundance of the nitrogen fixation(nif H),carbon fixation(cbb L),sulfur oxidation(sox B),and arsenite oxidation(aio A) genes were observed in the tailings,but not in the *** reconstructed nif-containing metagenome-assembled genomes(MAGs) suggest that the carbon fixation and sulfur oxidation pathways were important for potential diazotrophs inhabiting the *** measurements further confirmed that diazotrophs inhabiting tailings preferentially to use the inorganic electron donors(e.g.,elemental sulfur) compared to organic electron donors(e.g.,sucrose),while diazotrophs inhabiting soils preferred organic carbon ***,these findings suggest that chemolithoautotrophic diazotrophs may play essential roles in acquiring nutrients and facilitating ecological succession in tailings.