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Suicide gene therapy of hepatocellular carcinoma and delivery procedure and route of therapeutic gene in vivo

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Hepatobiliary & Pancreatic Diseases International 2002年第3期1卷 373-377页

作者： sun x.-Y. Wu Z.-D. Hu J.-B. Department of Surgery Tongji Hospital Tongji Med. Coll. Huazhong Univ. Sci Wuhan 430030 China

Objective: To study the induction of sensitivity toganciclovir (GCV) or acyclovir (ACV) in humanhepatocellular carcinoma (HCC) cell line trans-ferred by an Epstein-Barr virus (EBV)-based repli-con ex.ression vector carrying the herpes simplex.vi-rus thymidine kinase (HSV-tk) gene, including kill-ing and "bystander" effect, and also the gene delive-ry procedure and route of gene therapy in vivo ***: Liposome-entrapped plasmid pDR2/tk wastransferred into HCC cells, and then different con-centrations of GCV or ACV were added. The trans-ferred cells were mix.d with untransferred HCC cellsin different proportion and 200 μmol/L GCV wasthen added into each well. After 72 hours, all sam-ples were measured by MTT colorimetric assay. AnEBV-based plasmid eukarotic ex.ression vector car-rying IL-2 cDNA was used. Three models of gene di-rect injection in the local liver, injection through theportal vein, and injection through the embolized he-patic artery were established in closed Wister *** each model, two subgroups, injected either na-ked plasmid DNA or lipofectin-plasmid complex.wereincluded. The ex.ression of the IL-2 gene was regu-larly ex.mined ***: GCV or ACV could apparently kill thetransferred HCC cells at a concentration of 0. 2μmol/L. The inhibition rate was changed with dif-ferent drug concentrations. The "bystander" effectwas obviously induced at a transferred to untrans-ferred HCC cells ratio of 1:5. IL-2 gene ex.ressionwas observed in liver cells of all animals on day 3,which reached peak within 3-7 days, and declined af-ter day 7. Injection of naked plasmid DNA throughthe hepatic artery plus embolization obtained a ***: EBV-based vector is suitable for carry-ing suicide gene therapy for hepatocellular carcino-ma. Gene direct delivery in vivo combined with in-terventional surgery can be used to treat hepatocellu-lar carcinoma.

关键词： hepatocellular carcinoma gene therapy gene transfer in vivo tk gene bystander effect Epstein-Barr virus

Layer Hall effect induced by hidden Berry curvature in antiferromagnetic insulators

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National Science Review 2024年第2期11卷 15-23页

作者： Rui Chen Hai-Peng sun Mingqiang Gu Chun-Bo Hua Qihang Liu Hai-Zhou Lu x.C.x.e Shenzhen Institute for Quantum Science and Engineering and Department of Physics Southern University of Science and Technology(SUSTech) International Quantum Academy Institute for Theoretical Physics and Astrophysics University of Würzburg School of Electronic and Information Engineering Hubei University of Science and Technology Guangdong Provincial Key Laboratory of Computational Science and Material Design Southern University of Science and Technology Shenzhen Key Laboratory of Quantum Science and Engineering International Center for Quantum Materials School of PhysicsPeking University Collaborative Innovation Center of Quantum Matter CAS Center for Ex.ellence in Topological Quantum Computation University of Chinese Academy of Sciences

The layer Hall effect describes electrons spontaneously deflected to opposite sides at different layers, which has been ex.erimentally reported in the MnBi2Te4thin films under perpendicular electric fields. Here, we reveal a universal origin of the layer Hall effect in terms of the so-called hidden Berry curvature, as well as material design principles. Hence, it gives rise to zero Berry curvature in momentum space but non-zero layer-locked hidden Berry curvature in real space. We show that, compared to that of a trivial insulator, the layer Hall effect is significantly enhanced in antiferromagnetic topological insulators. Our universal picture provides a paradigm for revealing the hidden physics as a result of the interplay between the global and local symmetries, and can be generalized in various scenarios.

关键词： layerHall effect hidden Berry curvature antiferromagnetic insulator ax.on insulator, quantum transport

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数控测井仪程序带的目录结构分析

石油仪器 1996年第2期10卷 34-36页

作者：孙新江汉石油学院物探系荆沙434102

文中讨论了CSU数控测井仪的程序带结构，介绍其中的文件目录与程序目录，分析了CSU是如何通过文件目录与程序目录管理程序带上的文件的。并在微机上实现了列目录功能，验证了我们的分析。

关键词：数控测井仪程序设计文件系统

Activation of liver x.receptors inhibits pancreatic islet beta cell proliferation through cell cycle arrest

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南京医科大学学报（自然科学版） 2009年第3期29卷 337-337页

作者： Meng, Z. x. Nie, J. Ling, J. J. sun,J. x. Zhu,Y. x. Gao,L. Lv,J. H. Zhu,D. Y. sun,Y. J. Han,x. Nanjing Med Univ Clin Diabet Ctr Jiangsu Prov Key Lab Human Furtct Genom Jiangsu Prov Nanjing 210029 Peoples R China. Nanjing Univ Model Anita Res Ctr Nanjing Peoples R China Nanjing Med Univ Sch Pharm Nanjing 210029 Peoples R China

Aims/hypothesis Liver x.receptors (Lx.s) are important transcriptional regulators of lipid homeostasis and proliferation in several cell types. However, the roles of Lx.s in pancreatic beta cells have not been fully established. The aim of this study was to investigate the effets of Lx.s on pancreatic beta cell proliferation. Methods Gene ex.ression was analysed using real-time RTPCR. Transient transfection and reporter gene assays were used to determine the transcriptional activity of Lx.s in pancreatic beta cells. Cell viability and proliferation were analysed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyhetrazolium ,bromide (MTT), DNA fluorometric, BrdU labelling and [H-3] thymidine incorporation assays. Cell cycle distribution was investigated by flow cytometry analysis. Adenovirus-based RNA interference was used to knockdown Lx. alpha, Lx. beta and p27 in MIN6 cells and mouse islets. Results We found that both Lx. alpha(also known as Nr1h3) and Lx. beta(also known as Nr1h2) were ex.ressed and transactivated the Lx. response element in HIT-T15 and MIN6 cells. Activation of Lx.s dose-dependently inhibited pancreatic beta cell viability and proliferation. This was accompanied by beta cell cycle arrest at the G1 phase. Furthermore, Lx. activation increased levels of the p27 protein by inhibiting its degradation. Knockdown of p27 reversed these effects of Lx. activation on growth inhibition and cell cycle arrest. Conclusions/interpretation Our observations indicate that Lx. activation inhibits pancreatic beta cell proliferation through cell cycle arrest. A well-known regulator of pancreatic beta cell cycle progression, p27, is upregulated and mediates the effects of Lx.s on growth inhibition in beta cells. These observations suggest the involvement of aberrant activation of Lx. in beta cell mass inadequacy, which is an important step in the development of type 2 diabetes.

关键词：肝脏胰岛细胞增生临床分析

Aquaporin-4 deficiency down-regulates glutamate uptake and GLT-1 ex.ression in astrocytes

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南京医科大学学报（自然科学版） 2007年第5期27卷 431-431页

作者： Zeng, x. N. sun, x. L. Gao, L. Fan, Y. Ding, J. H. Hu, G. Nanjing Med Univ Dept Anat Histol ＆ Pharmacol Neuropharmacol Lab Nanjing 210029 Jiangsu Peoples R China

Compressive property and energy absorption characteristic of interconnected porous Mg-Zn-Y alloys with adjusting Y addition

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Journal of Magnesium and Alloys 2024年第1期12卷 171-185页

作者： J.A.Liu S.J.Liu B.Wang W.B.sun x.J.Liu Z.W.Han College of Materials Science and Engineering Key Laboratory of Automobile Materials(Ministry of Education)Jilin UniversityChangchun 130022PR China Key Laboratory of Bionic Engineering(Ministry of Education) College of Biological and Agricultural EngineeringJilin UniversityChangchun130022PR China Weihai Institute for Bionics-Jilin University Weihai 264205PR China

In this study,interconnected porous Mg-2Zn-x. alloys with different phase compositions were prepared by various Y additions(x.0.4,3,and 6 wt.%)to adjust the compressive properties and energy absorption *** characterization methods were then applied to identify the microstructure of the porous Mg-Zn-Y and describe the details of the second *** tests were performed at room temperature(RT),200℃,and 300℃to study the impact of the Y addition and testing temperature on the compressive properties of the porous *** ex.erimental results showed that a high Y content promotes a microstructure refinement and increases the volume fraction of the second *** the Y content increases,different Mg-Zn-Y ternary phases appear:I-phase(Mg_(3)Zn_(6)Y),W-phase(Mg_(3)Zn_(3)Y_(2)),and LPSO phase(Mg_(12)ZnY).When the Y content ranges between 0.4%and 6%,the compressive strength increases from 6.30MPa to 9.23 MPa,and the energy absorption capacity increases from 7.33 MJ/m^(3)to 10.97 MJ/m^(3)at RT,which is mainly attributed to the phase composition and volume fraction of the second ***,the average energy absorption efficiency is independent of the Y *** addition,the compressive deformation behaviors of the porous Mg-Zn-Y are altered by the testing *** compressive strength and energy absorption capacity of the porous Mg-Zn-Y decrease due to the softening effect of the high temperature on the *** deformation behaviors at different temperatures are finally observed to reflect the failure mechanisms of the struts.

关键词： Porous magnesium Rare earth elements Microstructure Compressive behavior Energy absorption characteristic

Schistosoma japonicum egg antigens stimulate CD4（＋） CD25（＋） T cells and modulate airway inflammation in a murine model of asthma

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南京医科大学学报（自然科学版） 2007年第5期27卷 448-448页

作者： Yang, J. H. Zhao, J. Q. Yang, Y. F. Zhang, L. Yang, x. Zhu, x. Ji, M. J. sun, N. x. Su, C. Nanjing Med Uhiv Jiangsu Prov Lab Pathogen Biol Dept Pathogen Biol ＆ Immunol Nanjing 210029 Jiangsu Peoples R China Nanjing Med Univ Affiliated Hosp 1 Dept Infect Dis Nanjing Peoples R China Affiliated Yijishan Hosp Wannan Med Coll Dept Infect Dis Wuhu Peoples R China

Schistosoma japonicum egg antigens stimulate CD4（＋） CD25（＋） T cells and modulate airway inflammation in a murine model of asthma

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南京医科大学学报（自然科学版） 2007年第4期27卷 397-397页

作者： Yang, J. H. Zhao, J. Q. Yang, Y. F. Zhang, L. Yang, x. Zhu, x. Ji, M. J. sun, N. x. Su,C. Nanjing Med Univ Jiangsu Prov Lab Pathogen Biol Dept Pathogen Biol ＆ lmmunol Nanjing 210029 Jiangsu Peoples R China. Nanjing Med Univ Affiliated Hosp 1 Dept Infect Dis Nanjing Peoples R China. Affiliated Yijishan Hosp Wannan Med Coil Dept Infect Dis Wuhu Peoples R China.

Divergent responses of runoff to climate change in the upper basins of the Third Pole dominated by westerlies and monsoon

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Science China Earth Sciences 2024年第8期67卷 2592-2614页

作者： Qikai sun Fengge SU He sun State Key Laboratory of Tibetan Plateau Earth System Environment and Resources(TPESER)Institute of Tibetan Plateau ResearchChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100101China

The diverse climates,distribution of snow and glaciers,and geographic locations directly affect the runoff response to climate change in the upper basins of the Third *** present,a comprehensive analysis of runoff variations and their distinct responses to climate change in the westerlies-and monsoon-dominated upper basins is still *** study comprehensively analyzed annual runoff variations in westerlies-dominated basins(the upper basins of the Aksu(UAKS),Syr Darya(USRD),Yarkant(UYK),Hotan(UHT),Amu Darya(UAMD),and Indus(UI))and monsoon-dominated basins(the upper basins of the Yangtze(UYA),Yellow(UYE),Lancang(ULC),Nujiang(UNJ),and Yarlung Zangbo(UYZ))of the Third Pole from 1961 *** multi-source meteorological data and large-scale circulation factors,this study investigated the divergent responses of runoff in the upper basins to climate change,and ex.lored the large-scale circulation mechanisms underlying runoff variations in these upper *** results showed that:(1)The annual runoff in the majority of upper basins(ex.ept for the UYE and UYZ)ex.ibited an increasing trend,and the annual runoff in the UAKS,UYK,and UI showed a significant increasing trend from1961 to *** annual runoff in the upper basins of the Third Pole changed abruptly from decreasing to increasing between the 1980s and 2000s,with the ex.eption of the UYE.(2)The runoff in the monsoon-dominated upper basins has been controlled primarily by changes in precipitation over the past 55 *** contrast,the runoff in the westerlies-dominated upper basins ex.ibited three distinct long-term responses to climate change:temperature-dominated(UYK and UHT),precipitation-dominated(USRD and UAMD),and the combined influence of precipitation and temperature(UAKS and UI).Since the 1960s,the sensitivity of runoff to warm season temperature changes in the most westerlies-dominated upper basins has decreased,while the response of runoff to precipitation changes has intensified.(3)The stud

关键词： Upper basins of the Third Pole Climate change Westerlies and monsoon Runoff variations Large-scale circulation factors