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New design to remove leukocytes from platelet-rich plasma(PRP)based on cell dimension rather than density

Bioactive Materials 2021年第10期6卷 3528-3540页

作者： subhaini jakfar Tzu-Chieh Lin Shinn-Chih Wu Yao-Horng Wang Yu-Jun Sun Minal Thacker Li-Xin Liu Feng-Huei Lin Institute of Biomedical Engineering National Taiwan UniversityTaipei106China Dentistry Faculty Syiah Kuala UniversityDarussalam23111Banda AcehIndonesia Department of Animal Science and Technology National Taiwan UniversityTaipeiChina Department of Nursing Yuanpei University of Medical Technology306Yuanpei StreetHsinchu300China School of Materials Science and Engineering Center of Functional BiomaterialsKey Laboratory of Polymeric Composite Materials and Functional Materials of EducationGD Research Center for Functional Biomaterials Engineering and TechnologySun Yat-Sen UnversityGuangzhou510275China Institute of Biomedical Engineering and Nanomedicine National Health Research InstitutesMiaoli350China

Platelet-rich plasma(PRP)can stimulate the proliferation of stem cells and have a positive effect on tissue *** many commercialized PRP preparation kits are already on the market,first-line clinical workers are still not satisfied with most of the PRP *** work of commercial PRP kits is based on the density of blood ***,the blood elements are very close in density which makes the separation ***,the mentioned commercialized kits are generally contaminated by leucocytes and *** this study,a home-designed PRP device was developed to use a separation membrane with adequate cut-off pore size of 5μm,3μm and 2μm for the groups of H5M,H3M,and H2M,respectively,to be placed in the middle of the centrifuge *** home-designed H2M showed a very promising results regardless of the final volume(1.82±0.09 ml),platelet yield(8.39±0.44%),Red Blood Cells(0%),White Blood Cells(0%),and Relative Concentration of Platelet Increment value(225.09%).Further,it showed a good result in cell viability and cytotoxicity and confirmed a good multilineage *** concentration in PRP prepared by group H2M was relatively stable and far above *** the fibrin fibers were linked together as bridging strands or strings and turned into an inter-connected porous structure for nutrients transportation and regenerative cell *** believe that the home-designed group H2M should have a great potential to develop into the final product to meet the requirements of first-line clinical workers.

关键词： Platelet-rich plasma(PRP) Filtered-tube design Homemade design Cut-off pore size Platelets Growth factors

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The development of laminin-alginate microspheres encapsulated with Ginsenoside Rg1 and ADSCs for breast reconstruction after lumpectomy

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Bioactive Materials 2021年第6期6卷 1699-1710页

作者： I-Hsuan Yang Yo-Shen Chen Jia-Jing Li Ya-Jyun Liang Tzu-Chieh Lin subhaini jakfar Minal Thacker Shinn-Chih Wu Feng-Huei Lin Department of Biomedical Engineering College of Medicine and College of EngineeringNational Taiwan UniversityNo.49Fanglan RdTaipei10672China Division of Plastic Surgery Department of SurgeryFar Eastern Memorial HospitalNo.21Sec.2Nanya S.Rd.New Taipei City220China Department of Animal Science and Technology National Taiwan UniversityNo.1Section 4Roosevelt RoadTaipei City10617China Institute of Biomedical Engineering and Nanomedicine National Health Research InstitutesNo.35Keyan RoadZhunanMiaoli County35053China

Many technologies have been developed for breast reconstruction after *** the technologies achieved promising success in clinical,there are still many shortages hanging over and trouble the *** engineering technology was introduced to plastic surgery that gave a light to lumpectomy patients in breast *** unexpected absorption rate,resulting from limited vascularization and low cell survival rate,is a major factor that leads to unsatisfactory results for the previous studies in our *** the study,the laminin-modified alginate synthesized by a new method of low concertation of sodium periodate would be mixed with ADSCs and Rg1 in the medium;and then sprayed into a calcium chloride(CaCl2)solution to prepare into microsphere(abbreviated as ADSC-G-LAMS)by bio-electrospray with a power syringe for the mass production and smaller bead *** developed ADSC-G-LAMS microspheres had the diameter of 232±42μ***-release of the Rg1 retained its biological ***-1,live/dead staining,and chromosome aberration assay were evaluated to confirm the safety of the *** in vivo study,ADSC-G-LAMS microspheres combined with autologous adipocytes were transplanted into the dorsum of rats by subcutaneous *** efficacy was investigated by H&E and immunofluorescence *** results showed that the bioactive ADSC-G-LAMS microspheres could integrate well into the host adipose tissue with an adequate rate of angiogenesis by constantly releasing Rg1 to enhance the ADSC or adipocyte survival rate to join tissue growth and repair with adipogenesis for breast reconstruction after lumpectomy.

关键词： Laminin-alginate microspheres Adipose-derived stem cells Ginsenoside Rg1 Bio-electrospray Tissue engineering

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