New design to remove leukocytes from platelet-rich plasma(PRP)based on cell dimension rather than density
作者机构：Institute of Biomedical EngineeringNational Taiwan UniversityTaipei106China Dentistry FacultySyiah Kuala UniversityDarussalam23111Banda AcehIndonesia Department of Animal Science and TechnologyNational Taiwan UniversityTaipeiChina Department of NursingYuanpei University of Medical Technology306Yuanpei StreetHsinchu300China School of Materials Science and EngineeringCenter of Functional BiomaterialsKey Laboratory of Polymeric Composite Materials and Functional Materials of EducationGD Research Center for Functional Biomaterials Engineering and TechnologySun Yat-Sen UnversityGuangzhou510275China Institute of Biomedical Engineering and NanomedicineNational Health Research InstitutesMiaoli350China
出 版 物：《Bioactive Materials》 (生物活性材料（英文）)
年 卷 期：2021年第6卷第10期
页 面：3528-3540页
核心收录：
学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程]
基 金：This work was supported by the Indonesia Endowment Fund for Education(LPDP)within the ministry of Finance Indonesia(grant number PRJ-317/LPDP.3/2016)
主 题：Platelet-rich plasma(PRP) Filtered-tube design Homemade design Cut-off pore size Platelets Growth factors
摘 要：Platelet-rich plasma(PRP)can stimulate the proliferation of stem cells and have a positive effect on tissue *** many commercialized PRP preparation kits are already on the market,first-line clinical workers are still not satisfied with most of the PRP *** work of commercial PRP kits is based on the density of blood ***,the blood elements are very close in density which makes the separation ***,the mentioned commercialized kits are generally contaminated by leucocytes and *** this study,a home-designed PRP device was developed to use a separation membrane with adequate cut-off pore size of 5μm,3μm and 2μm for the groups of H5M,H3M,and H2M,respectively,to be placed in the middle of the centrifuge *** home-designed H2M showed a very promising results regardless of the final volume(1.82±0.09 ml),platelet yield(8.39±0.44%),Red Blood Cells(0%),White Blood Cells(0%),and Relative Concentration of Platelet Increment value(225.09%).Further,it showed a good result in cell viability and cytotoxicity and confirmed a good multilineage *** concentration in PRP prepared by group H2M was relatively stable and far above *** the fibrin fibers were linked together as bridging strands or strings and turned into an inter-connected porous structure for nutrients transportation and regenerative cell *** believe that the home-designed group H2M should have a great potential to develop into the final product to meet the requirements of first-line clinical workers.
维普期刊数据库