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Presence of cryptosporidium parvum and Giardia lamblia in water samples from Southeast Asia: towards an integrated water detection system

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Infectious Diseases of Poverty 2016年第1期5卷 15-26页

作者： Thulasi Kumar Mohamad Azlan Abd Majid subashini Onichandran Narong Jaturas Hemah Andiappan cristina c.Salibay Hazel A.L.Tabo Norbel Tabo Julieta Z.Dungca Jitbanjong Tangpong Sucheep Phiriyasamith Boonyaorn Yuttayong Raxsina Polseela Binh Nhu Do Nongyao Sawangjaroen Tian-chye Tan Yvonne A.L.Lim Veeranoot Nissapatorn Department of Parasitology(Southeast Asia Water Team) Faculty of MedicineUniversity of MalayaKuala LumpurMalaysia Biological Science Department College of Science and Computer StudiesDe La Salle University-DasmariñasDasmariñasPhilippines School of Science and Technology Centro Escolar UniversityManilaPhilippines School of Allied Health Sciences and Public Health Walailak UniversityNakhon Si ThammaratThailand. Graduate School Kasem Bundit UniversityBangkokThailand. Regional Medical Sciences center Department of Medical SciencesMinistry of Public HealthNakhon RatchasimaThailand. Department of Microbiology and Parasitology Faculty of Medical ScienceNaresuan UniversityPhitsanulokThailand. Department of Parasitology Faculty of MedicineVietnam Military Medical University160 Phung Hung RoadPhuc La WardHa Dong DistrictHanoiVietnam Department of Microbiology Faculty of SciencePrince of Songkla UniversityHat YaiThailand

Background:Access to clean and safe drinking water that is free from pathogenic protozoan parasites,especially cryptosporidium parvum and Giardia lamblia that cause gastrointestinal illness in humans,is still an issue in Southeast Asia(SEA).This study is the first attempt to detect the aforementioned protozoan parasites in water samples from countries in SEA,using real-time polymerase chain reaction(qPcR)***:A total of 221 water samples of 10 l each were collected between April and October 2013 from Malaysia(53),Thailand(120),the Philippines(33),and Vietnam(15).A physicochemical analysis was *** water samples were processed in accordance with the US Environmental Protection Agency’s methods 1622/1623.1,microscopically observed and subsequently screened using qPcR ***:cryptosporidium oocysts were detected in treated water samples from the Philippines(1/10),with a concentration of 0.06±0.19 oocyst/L,and untreated water samples from Thailand(25/93),Malaysia(17/44),and the Philippines(11/23),with concentrations ranging from 0.13±0.18 to 0.57±1.41 oocyst/*** cysts were found in treated water samples from the Philippines(1/10),with a concentration of 0.02±0.06 cyst/L,and in untreated water samples from Thailand(20/93),Vietnam(5/10),Malaysia(22/44),and the Philippines(16/23),with concentrations ranging from 0.12±0.3 to 8.90±19.65 cyst/*** pathogens *** and *** were detected using using qPcR assays by targeting the 138-bp fragment and the small subunit gene,*** was detected in untreated water samples from the Philippines(1/23)and Malaysia(2/44),whilst,*** detected was detected in treated water samples from the Philippines(1/10)and in untreated water samples from Thailand(21/93),Malaysia(12/44),and the Philippines(17/23).Nitrate concentration was found to have a high positive correlation with(oo)cyst(0.993).conclusion:The presence of(oo)cysts in the water samples means that there is potential risk for

关键词： cryptosporidium parvum Giardia lamblia Physicochemical Microscopy Real-time polymerase chain reaction Southeast Asia

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One stop shop Ⅱ:taxonomic update with molecular phylogeny for important phytopathogenic genera:26–50(2019)

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Fungal Diversity 2019年第1期 41-129页

作者： Ruvishika S.Jayawardena Kevin D.Hyde Rajesh Jeewon Masoomeh Ghobad-Nejhad Dhanushka N.Wanasinghe NingGuo Liu Alan J.L.Phillips Jose Ribamar cOliveira-Filho Gladstone Ada Silva Tatiana B.Gibertoni P.Abeywikrama L.M.carris K.W.T.chethana A.J.Dissanayake S.Hongsanan S.c.jayasiri A.R.McTaggart R.H.Perera K.Phutthacharoen K.G.Savchenko R.G.Shivas Naritsada Thongklang Wei Dong DePing Wei Nalin NWijayawardena Ji-chuan Kang The Engineering Research center of Southwest Biopharmaceutical Resources Ministry of EducationGuizhou UniversityGuiyang 550025People’s Republic of China center of Excellence in Fungal Research Mae Fah Luang UniversityChiang RaiThailand Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming 650201People’s Republic of China Department of Health Sciences Faculty of ScienceUniversity of MauritiusReduitMauritius Department of Biotechnology Iranian Research Organization for Science and Technology(IROST)P.O.Box 15815-3538Tehran 15819Iran World Agroforestry centre East and Central AsiaKunming 650201YunnanPeople’s Republic of China Universidade de Lisboa Faculdade de CienciasBiosystems and Integrative Sciences Institute(BioISI)Campo Grande1749-016 LisbonPortugal Departamento de Micologia Universidade Federal de PernambucoAvenida da EngenhariaS/N-Cidade UniversitariaRecifePE 50740-600Brazil Beijing Key Laboratory of Environmet Friendly Management on Fruit Disease and Pests in North china Institute of Plant and Environment ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097People’s Republic of China Department of Plant Pathology Washington State UniversityPullmanWA 99164USA college of Life Science and Oceanography ShenZhen University1068Nanhai AvenueNanshanShenzhen 518055China Queensland Alliance for Agriculture and Food Innovation The University of QueenslandBrisbaneQLD 4001Australia Department of Biological Sciences Butler UniversityIndianapolisIN 46208USA centre for crop Health University of Southern QueenslandToowoombaQLD 4350Australia Department of Entomology and Plant Pathology Faculty of AgricultureChiang Mai UniversityChiang Mai 50200Thailand Faculty of Agriculture National Resources and EnvironmentNaresuan UniversityPhitsanulok 65000Thailand

This paper is the second in a series focused on providing a stable platform for the taxonomy of phytopathogenic *** focuses on 25 phytopathogenic genera:Alternaria,Bipolaris,Boeremia,Botryosphaeria,calonectria,coniella,corticiaceae,curvularia,Elsinoe,Entyloma,Erythricium,Fomitiporia,Fulviformes,Laetisaria,Limonomyces,Neofabraea,Neofusicoccum,Phaeoacremonium,Phellinotus,Phyllosticta,Plenodomus,Pseudopyricularia,Tilletia,Venturia and Waitea,using recent molecular data,up to date names and the latest taxonomic *** each genus a taxonomic background,diversity aspects,species identification and classification based on molecular phylogeny and recommended genetic markers are *** this study,varieties of the genus Boeremia have been elevated to species ***,Bipolaris,curvularia,Neofusicoccum and Phyllosticta that were included in the One Stop Shop 1 paper are provided with updated entries,as many new species have been introduced to these genera.

关键词： Boeremia DNA barcodes Endophytes Plant pathology Systematics Taxonomy

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Turbo-taxonomy to assemble a megadiverse lichen genus:seventy new species of cora(Basidiomycota:Agaricales:Hygrophoraceae),honouring David Leslie Hawksworth’s seventieth birthday

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Fungal Diversity 2017年第3期84卷 139-207页

作者： Robert Lücking Manuela Dal Forno Bibiana Moncada Luis Fernando coca Leidy Yasmín Vargas-Mendoza André Aptroot Laura Juliana Arias Betty Besal Frank Bungartz Diego Mauricio cabrera-Amaya Marcela E.S.cáceres José Luis chaves Sionara Eliasaro Martha cecilia Gutiérrez Jesús E.Hernández Marin María de los Ángeles Herrera-campos María E.Holgado-Rojas Harald Jonitz Martin Kukwa Fabiane Lucheta Santiago Madriñán Marcelo Pinto Marcelli Suzana Maria de Azevedo Martins Joel A.Mercado-Díaz Jorge Alberto Molina Eduardo A.Morales Peter R.Nelson Freddy Nugra Francisco Ortega Telma Paredes Ayda Lucía Patiño Rouchi Nadine Peláez-Pulido Rosa Emilia Pérez Pérez Gary B.Perlmutter Eimy Rivas-Plata Javier Robayo camilo Rodríguez Diego Fernando Simijaca Edier Soto-Medina Adriano Afonso Spielmann Alejandra Suárez-corredor Jean-Marc Torres carlos Alberto Vargas Alba Yánez-Ayabaca Gothamie Weerakoon Karina Wilk Marcela celis Pacheco Mauricio Diazgranados Grischa Brokamp Thomas Borsch Patrick M.Gillevet Masoumeh Sikaroodi James D.Lawrey Botanical Garden and Botanical Museum Berlin KöniginLuise-Straße 6-814195 BerlinGermany Science Education The Field Museum1400 South Lake ShoreChicagoIL 60605USA Department of Botany Smithsonian InstitutionNational Museum of Natural History10th St Constitution Ave NWWashingtonDC 20560USA Department of Environmental Science and Policy George Mason UniversityFairfaxVA 22030-4444USA Licenciatura en Biologı´a Universidad Distrital Francisco Jose´de CaldasCra.4 No.26D-54Torre de LaboratoriosHerbarioBogota´D.C.Colombia Herbario Universidad de caldas(FAUc) Edificio BicentenarioCalle 65No.26-10ManizalesA.A.275CaldasColombia ABL Herbarium G.v.d.Veenstraat 1073762 XK SoestThe Netherlands Biologı´a Universidad del Quindı´oCarrera 15 Calle 12 NorteArmeniaQuindı´oColombia 120 chavis Avenue LexingtonVA 24450USA charles Darwin Foundation Puerto AyoraSanta Cruz IslandGala´pagosEcuador Schedestraße 8 53113 BonnGermany Jardı´n Bota´nico de Bogota´Jose´celestino Mutis Avenida Calle 63 No.68-95Bogota´D.C.Colombia Departamento de Biocieˆncias Universidade Federal de SergipeAvenida Vereador Olı´mpio Grandes/n.ItabaianaCEP:49.500-000 SergipeBrazil Laboratorio de Hongos Instituto Nacional de Biodiversidad(INBio)Apdo.22-3100Santo Domingo de HerediaCosta Rica Departamento de Botaˆnica Universidade Federal do Parana´Campus Polite´cnicoSetor de Cieˆncias Biolo´gicasAv.Cel.Francisco H.dos Santoss/nJardim das Ame´ricas81531-980 CuritibaPRBrazil Ingenierı´a Ambiental Universidad Distrital Francisco Jose´de CaldasCarrera 5 Este No.15-82Av.CircumvalarBogota´D.C.Colombia Instituto Experimental Jardı´n Bota´nico Dr.Tobı´as Lasser Centro de Investigacio´n y DesarrolloUniversidad Central de VenezuelaAve.Salvador AllendeJardı´n Bota´nico de CaracasApartado#2156Caracas 1010-AVenezuela Departamento de Bota´nica Instituto de Biologı´aUniversidad Nacional Auto´noma de Me´xicoApartado Postal 70-36704510 Ciudad De Me´xicoMexico Facultad de ciencias Escuela Profesional de Biologı´aUniv

Following a large-scale phylogenetic study of the lichenized genus cora(Basidiomycota:Agaricales:Hygrophoraceae),we formally describe 70 new species,honouring the seventieth birthday of David Leslie Hawksworth,one of the preeminent figures in mycology and lichenology in the past 50 *** on an updated phylogeny using the ITS fungal barcoding locus,we now recognize 189 taxa in a genus that until recently was considered to represent a single species;including this contribution,92 of these are formally recognized,including five taxa based on historical names or collections that have not been *** of cora can be recognized by a combination of morphological(size,colour,lobe configuration,surface hairs,hymenophore size and shape),anatomical(thallus thickness,cortex structure,photobiont type,hyphal papillae),and ecogeographical features(substrate,habitat,distribution),and a keytable allowing the identification of all accepted taxa is *** new species are:cora accipiter Moncada,Madrin˜a´n&Lücking ***.,*** Moncada,Soto-Medina&Lücking ***.,*** Moncada,Dal Forno&Lücking ***.,*** Dal Forno,chaves&Lücking ***.,*** Moncada,***ı´guez&Lücking ***.,*** Lücking,Moncada&*** ***.,*** Moncada,Ya´nez-Ayabaca&Lücking ***.,*** Moncada,Patin˜o&Lücking ***.,*** Lücking,***&Dal Forno ***.,*** Holgado,Rivas Plata&Perlmutter ***.,*** Dal Forno,Eliasaro&Spielmann ***.,*** Nugra,Dal Forno&Lücking ***.,*** Lücking,Martins&Lucheta ***.,*** Moncada,R.-***´rez&Lücking ***.,*** Moncada,***.&Lücking ***.,*** Moncada,cabreraAmaya&Lücking ***.,*** Moncada,R.-***´rez&***.,*** Lücking,***&Dal Forno ***.,*** Moncada,***´rez-corredor&Lücking ***.,*** Moncada,***&Lücking ***.,***

关键词： Bolivia citizen science chile Mexico Puerto Rico Sri Lanka

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